LOCUS       NM_178170               3573 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens NIMA related kinase 8 (NEK8), mRNA.
ACCESSION   NM_178170
VERSION     NM_178170.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Claus,L.R., Chen,C., Stallworth,J., Turner,J.L., Slaats,G.G.,
            Hawks,A.L., Mabillard,H., Senum,S.R., Srikanth,S.,
            Flanagan-Steet,H., Louie,R.J., Silver,J., Lerner-Ellis,J.,
            Morel,C., Mighton,C., Sleutels,F., van Slegtenhorst,M., van Ham,T.,
            Brooks,A.S., Dorresteijn,E.M., Barakat,T.S., Dahan,K., Demoulin,N.,
            Goffin,E.J., Olinger,E., Larsen,M., Hertz,J.M., Lilien,M.R.,
            Obeidova,L., Seeman,T., Stone,H.K., Kerecuk,L., Gurgu,M., Yousef
            Yengej,F.A., Ammerlaan,C.M.E., Rookmaaker,M.B., Hanna,C.,
            Rogers,R.C., Duran,K., Peters,E., Sayer,J.A., van Haaften,G.,
            Harris,P.C., Ling,K., Mason,J.M., van Eerde,A.M. and Steet,R.
  CONSRTM   Genomics England Research Consortium
  TITLE     Certain heterozygous variants in the kinase domain of the
            serine/threonine kinase NEK8 can cause an autosomal dominant form
            of polycystic kidney disease
  JOURNAL   Kidney Int 104 (5), 995-1007 (2023)
   PUBMED   37598857
  REMARK    GeneRIF: Certain heterozygous variants in the kinase domain of the
            serine/threonine kinase NEK8 can cause an autosomal dominant form
            of polycystic kidney disease.
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Cao,B., Zhang,K., Pan,C., Dong,Y. and Lu,F.
  TITLE     NEK8 regulates colorectal cancer progression via phosphorylating
            MYC
  JOURNAL   Cell Commun Signal 21 (1), 209 (2023)
   PUBMED   37596667
  REMARK    GeneRIF: NEK8 regulates colorectal cancer progression via
            phosphorylating MYC.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3573)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3573)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 3573)
  AUTHORS   Valkova,N., Yunis,R., Mak,S.K., Kang,K. and Kultz,D.
  TITLE     Nek8 mutation causes overexpression of galectin-1, sorcin, and
            vimentin and accumulation of the major urinary protein in renal
            cysts of jck mice
  JOURNAL   Mol Cell Proteomics 4 (7), 1009-1018 (2005)
   PUBMED   15872312
  REMARK    GeneRIF: characterization of the proteome in mice that have a
            double point mutation in the related gene.
REFERENCE   6  (bases 1 to 3573)
  AUTHORS   Barrios-Rodiles,M., Brown,K.R., Ozdamar,B., Bose,R., Liu,Z.,
            Donovan,R.S., Shinjo,F., Liu,Y., Dembowy,J., Taylor,I.W., Luga,V.,
            Przulj,N., Robinson,M., Suzuki,H., Hayashizaki,Y., Jurisica,I. and
            Wrana,J.L.
  TITLE     High-throughput mapping of a dynamic signaling network in mammalian
            cells
  JOURNAL   Science 307 (5715), 1621-1625 (2005)
   PUBMED   15761153
REFERENCE   7  (bases 1 to 3573)
  AUTHORS   Bowers,A.J. and Boylan,J.F.
  TITLE     Nek8, a NIMA family kinase member, is overexpressed in primary
            human breast tumors
  JOURNAL   Gene 328, 135-142 (2004)
   PUBMED   15019993
  REMARK    GeneRIF: Data demonstrate for the first time that Nek8 is a novel
            tumor associated gene, and shares considerable sequence homology
            with the Nek family of protein kinases and may be involved in
            G(2)/M progression.
REFERENCE   8  (bases 1 to 3573)
  AUTHORS   Holland,P.M., Milne,A., Garka,K., Johnson,R.S., Willis,C.,
            Sims,J.E., Rauch,C.T., Bird,T.A. and Virca,G.D.
  TITLE     Purification, cloning, and characterization of Nek8, a novel
            NIMA-related kinase, and its candidate substrate Bicd2
  JOURNAL   J Biol Chem 277 (18), 16229-16240 (2002)
   PUBMED   11864968
REFERENCE   9  (bases 1 to 3573)
  AUTHORS   Stokman,M., Lilien,M. and Knoers,N.
  TITLE     Nephronophthisis-Related Ciliopathies
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27336129
REFERENCE   10 (bases 1 to 3573)
  AUTHORS   Gunay-Aygun,M., Gahl,W.A. and Heller,T.
  TITLE     Congenital Hepatic Fibrosis Overview horizontal line RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301743
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010761.10, AY242354.1 and
            CV811826.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 8, 2018 this sequence version replaced NM_178170.2.
            
            Summary: This gene encodes a member of the serine/threionine
            protein kinase family related to NIMA (never in mitosis, gene A) of
            Aspergillus nidulans. The encoded protein may play a role in cell
            cycle progression from G2 to M phase. Mutations in the related
            mouse gene are associated with a disease phenotype that closely
            parallels the juvenile autosomal recessive form of polycystic
            kidney disease in humans. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY242354.1, SRR1803611.273324.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000268766.11/ ENSP00000268766.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC010761.10        80940-80965
            27-2884             AY242354.1         1-2858
            2885-3188           AC010761.10        94919-95222
            3189-3573           CV811826.1         21-405              c
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..3573
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /note="NIMA related kinase 8"
                     /db_xref="GeneID:284086"
                     /db_xref="HGNC:HGNC:13387"
                     /db_xref="MIM:609799"
     exon            1..73
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..2105
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /EC_number="2.7.11.1"
                     /note="NIMA-related kinase 12a; NIMA-family kinase NEK8;
                     serine/thrionine-protein kinase NEK8; nephrocystin 9;
                     nimA-related protein kinase 8; nima-related protein kinase
                     12a; NIMA (never in mitosis gene a)- related kinase 8;
                     never in mitosis A-related kinase 8"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek8"
                     /protein_id="NP_835464.1"
                     /db_xref="CCDS:CCDS32597.1"
                     /db_xref="GeneID:284086"
                     /db_xref="HGNC:HGNC:13387"
                     /db_xref="MIM:609799"
                     /translation="MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEE
                     RQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEE
                     ETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKA
                     YTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSG
                     TFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRR
                     AEKSVAPSNTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEV
                     VQVAAGRTQKAGVTRSGRLILWEAPPLGAGGGSLLPGAVEQPQPQFISRFLEGQSGVT
                     IKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVAC
                     GASHVLALSTERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQEAQRVVCGIDSSM
                     ILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSI
                     DLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRGSRAPCKVQGLEGIKMAMVACGDAF
                     TVAIGAESEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAV
                     RSVTDEPVPP"
     exon            74..279
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            280..512
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            513..644
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            645..853
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            854..915
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1097
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1248
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1325
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1443
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1594
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1758
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1917
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2076
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..3573
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3555..3560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /note="hexamer: AATAAA"
     polyA_site      3573
                     /gene="NEK8"
                     /gene_synonym="JCK; NEK12A; NPHP9; PKD8; RHPD2"
                     /note="major polyA site"
ORIGIN      
        1 acggaagtga aactctaaga aatgagatgg agaagtacga gcggatccga gtggtgggga
       61 gaggtgcctt cgggattgtg cacctgtgcc tgcgaaaggc tgaccagaag ctggtgatca
      121 tcaagcagat tccagtggaa cagatgacca aggaagagcg gcaggcagcc cagaatgagt
      181 gccaggtcct caagctgctc aaccacccca atgtcattga gtactacgag aacttcctgg
      241 aagacaaagc ccttatgatc gccatggaat atgcaccagg cggcactctg gctgagttca
      301 tccaaaagcg ctgtaattcc ctgctggagg aggagaccat cctgcacttc ttcgtgcaga
      361 tcctgcttgc actgcatcat gtgcacaccc acctcatcct gcaccgagac ctcaagaccc
      421 agaacatcct gcttgacaaa caccgcatgg tcgtcaagat cggtgatttc ggcatctcca
      481 agatccttag cagcaagagc aaggcctaca cggtggtggg taccccatgc tatatctccc
      541 ctgagctgtg tgagggcaag ccctacaacc agaagagtga catctgggcc ctgggctgtg
      601 tcctctacga gctggccagc ctcaagaggg ctttcgaggc tgcgaacttg ccagcactgg
      661 tgctgaagat catgagtggc acctttgcac ctatctctga ccggtacagc cctgagcttc
      721 gccagctggt cctgagtcta ctcagcctgg agcctgccca gcggccacca ctcagccaca
      781 tcatggcaca gcccctctgc atccgtgccc tcctcaacct ccacaccgac gtgggcagtg
      841 tccgcatgcg gagggcagag aagtccgtgg cccccagcaa cacagggagc aggaccacca
      901 gtgtccgctg cagaggtatc ccccggggac ctgtgaggcc agccatccca ccaccactgt
      961 cgtcagtgta tgcctggggt ggtgggctgg gcacccccct gcggctgcca atgctcaaca
     1021 cagaggtggt ccaggtggca gctgggcgca cgcagaaagc cggcgtcacg cgctctgggc
     1081 gtctcatcct gtgggaggcc ccacccctag gtgcaggcgg aggcagtctc cttcctgggg
     1141 cagtggagca gccacagccc cagttcatct cgcgtttcct ggagggccag tcgggtgtga
     1201 ccatcaagca cgtggcctgt ggggacttct tcactgcctg cctgactgac agaggcatca
     1261 tcatgacatt cggcagcggc agcaatgggt gcctaggcca tggcagcctc actgacatca
     1321 gccagcccac cattgtggag gctttgctgg gctatgaaat ggtgcaggtg gcctgtgggg
     1381 cctctcacgt gctggccctg tccactgagc gagaactatt tgcctggggc cgtggagaca
     1441 gcggcagact ggggctaggc accagggagt cccacagctg cccccagcag gtgcccatgc
     1501 ccccaggaca ggaagctcag cgagttgtat gtggtatcga ttcctccatg atcctcactg
     1561 tgcctggcca agccctagcc tgtgggagca acaggttcaa caagctgggc ctggaccacc
     1621 tctccctggg ggaggagcct gtcccccacc agcaagtgga ggaggccctg agcttcacac
     1681 tactaggctc tgcacccctg gaccaggagc ctctgctgag tatagacctg ggcactgctc
     1741 actcagctgc tgtgactgcc tcgggtgatt gctacacttt tggcagcaat cagcacggac
     1801 agttgggcac caatactcgc cgaggcagtc gggcaccctg taaggtccaa ggccttgagg
     1861 gcatcaagat ggcaatggta gcctgtgggg atgccttcac tgtagctatt ggggcagaga
     1921 gcgaagtgta ctcttggggc aaaggggcgc gaggtcgatt gggaaggagg gatgaggatg
     1981 ccggactccc tcggccagtg cagttggatg agacacaccc ttacacggtg acttccgtgt
     2041 cctgttgcca tggaaacacc ctcctggctg ttcgatcggt cacagatgag ccggtccccc
     2101 cctgaggcac ccggattcac ctctggacca ccctgatatt gcttctcctc tgaatgctct
     2161 gaaaagtgca ggtgcccaag gcatgacttg cagctgtctc ctggattgtg tcatcatggg
     2221 gtatcagggc tggcaaaacc cctgctctgc ttttggcctt ggatatggaa acctcaacca
     2281 ctttgttctc ctaccacctc tttgcccttc ctaccccttc ctcccttcag tcagtgtccc
     2341 cagggtccag cctggctaga gttagaaggc agacctagcc tttggaagca gggtggcctc
     2401 cagagccttg ccataaaaag gctgaaggca gccgggcgca gtggctcacg cctgtaatcc
     2461 cagcactttg ggaggctgag gcgggcggat cacgaggtca ggagatcgag accatcctgg
     2521 ctaacgtggt gaaaccccat ctctactaaa aatacaaaaa aattagctgg gcgtggtggc
     2581 acgcgcctgt agtcccagct gctcaggagg ctgaggcagg agaatggcgt aaacccctcc
     2641 agcctgggcg acagaaccag actccgtctc aaaaaaaaaa aaaaaacaaa aaaggctgaa
     2701 ggcagctgta gctccacaca gaccctaccc tttcccctag cccagttaga aaataagtct
     2761 gggccggcgg cggtggctca cacctgcata ggcagtgctt tggccaggag gatcccttga
     2821 tcccttgtgg ccaggagttg ggagaccagc ctggggacca tagcgagacc gctgtctcca
     2881 ccaaaaaaaa aaaaaaagaa gaaaataagg ctgggtgcgg tggctcacgc ctgtaattcc
     2941 agcactttgg gaggccaagg cgggcggatc acttgaggtc agtagactga ggccagcctg
     3001 gccaacatgg tgaaaccccg tctctactaa aaatacaaaa attaaccagg catggtgacg
     3061 ggcgcctgta atcccagcta ctctggaggc taaggcaaga gaatcccttg aacccgggag
     3121 gtgaagtttg cagtgagccg agatcgcacc actgcactcc agcctggatg acagagcaag
     3181 tctccctctc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gtatttggtg cttaccggga
     3241 ctctatctcc atactttgga gcagggcaag ccagggcctg cctggactgg ccattaggcc
     3301 tgcctagctt ctgcatagcc cggggctggg cggtccccgg gagtgctgcc acctccccta
     3361 cactttcatc ctgaggcagg atttgctaga cgtcggcttg cctctcttcc ccagcaggag
     3421 ggcaggagtc actaagtgct gcagaaaaga tccgcgctgg agattgcccg tcccccccat
     3481 gcctttcccc agaaacagtt gagagaatgg gtagctggtg ggaccagacc ggtttcccag
     3541 aggacaggaa ggccaataaa aatatcagct gcc
//
