LOCUS       NM_133494               4114 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens NIMA related kinase 7 (NEK7), mRNA.
ACCESSION   NM_133494
VERSION     NM_133494.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Nie,L., Fei,C., Fan,Y., Dang,F., Zhao,Z., Zhu,T., Wu,X., Dai,T.,
            Balasubramanian,A., Pan,J., Hu,Y., Luo,H.R., Wei,W. and Chen,J.
  TITLE     Consecutive palmitoylation and phosphorylation orchestrates NLRP3
            membrane trafficking and inflammasome activation
  JOURNAL   Mol Cell 84 (17), 3336-3353 (2024)
   PUBMED   39173637
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Chen,L., Liu,Y., Yue,S., Wang,H., Chen,J., Ma,W., Xu,W., Xu,M.,
            Chen,Z., Chen,X., Zhang,L. and Liang,C.
  TITLE     P2X7R Modulates NEK7-NLRP3 Interaction to Exacerbate Experimental
            Autoimmune Prostatitis via GSDMD-mediated Prostate Epithelial Cell
            Pyroptosis
  JOURNAL   Int J Biol Sci 20 (9), 3393-3411 (2024)
   PUBMED   38993566
  REMARK    GeneRIF: P2X7R Modulates NEK7-NLRP3 Interaction to Exacerbate
            Experimental Autoimmune Prostatitis via GSDMD-mediated Prostate
            Epithelial Cell Pyroptosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4114)
  AUTHORS   Zheng,S., Que,X., Wang,S., Zhou,Q., Xing,X., Chen,L., Hou,C.,
            Ma,J., An,P., Peng,Y., Yao,Y., Song,Q., Li,J., Zhang,P. and Pei,H.
  TITLE     ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7
            interaction and inflammasome activation
  JOURNAL   Mol Cell 83 (24), 4570-4585 (2023)
   PUBMED   38092000
  REMARK    GeneRIF: ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7
            interaction and inflammasome activation.
REFERENCE   4  (bases 1 to 4114)
  AUTHORS   Xiao,L., Magupalli,V.G. and Wu,H.
  TITLE     Cryo-EM structures of the active NLRP3 inflammasome disc
  JOURNAL   Nature 613 (7944), 595-600 (2023)
   PUBMED   36442502
REFERENCE   5  (bases 1 to 4114)
  AUTHORS   Machtens,D.A., Bresch,I.P., Eberhage,J., Reubold,T.F. and
            Eschenburg,S.
  TITLE     The Inflammasome Activity of NLRP3 Is Independent of NEK7 in HEK293
            Cells Co-Expressing ASC
  JOURNAL   Int J Mol Sci 23 (18), 10269 (2022)
   PUBMED   36142182
  REMARK    GeneRIF: The Inflammasome Activity of NLRP3 Is Independent of NEK7
            in HEK293 Cells Co-Expressing ASC.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4114)
  AUTHORS   Kim,S., Lee,K. and Rhee,K.
  TITLE     NEK7 is a centrosomal kinase critical for microtubule nucleation
  JOURNAL   Biochem Biophys Res Commun 360 (1), 56-62 (2007)
   PUBMED   17586473
  REMARK    GeneRIF: In consistent to the proposal, we observed a decrease in
            the centrosomal gamma-tubulin levels and reduction of the
            microtubule re-growth activity in the NEK7-suppressed cells.
REFERENCE   7  (bases 1 to 4114)
  AUTHORS   Yissachar,N., Salem,H., Tennenbaum,T. and Motro,B.
  TITLE     Nek7 kinase is enriched at the centrosome, and is required for
            proper spindle assembly and mitotic progression
  JOURNAL   FEBS Lett 580 (27), 6489-6495 (2006)
   PUBMED   17101132
  REMARK    GeneRIF: a role for Nek7 in regulating proper spindle assembly and
            mitotic progression.
REFERENCE   8  (bases 1 to 4114)
  AUTHORS   Belham,C., Roig,J., Caldwell,J.A., Aoyama,Y., Kemp,B.E., Comb,M.
            and Avruch,J.
  TITLE     A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the
            Nek6 and Nek7 kinases
  JOURNAL   J Biol Chem 278 (37), 34897-34909 (2003)
   PUBMED   12840024
  REMARK    GeneRIF: Activated by Nercc1/NEK9; part of the mitotic casscade.
REFERENCE   9  (bases 1 to 4114)
  AUTHORS   Kimura,M. and Okano,Y.
  TITLE     Identification and assignment of the human NIMA-related protein
            kinase 7 gene (NEK7) to human chromosome 1q31.3
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 33-38 (2001)
   PUBMED   11701951
REFERENCE   10 (bases 1 to 4114)
  AUTHORS   Kandli,M., Feige,E., Chen,A., Kilfin,G. and Motro,B.
  TITLE     Isolation and characterization of two evolutionarily conserved
            murine kinases (Nek6 and nek7) related to the fungal mitotic
            regulator, NIMA
  JOURNAL   Genomics 68 (2), 187-196 (2000)
   PUBMED   10964517
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB498485.1, AK313411.1 and AC093419.2.
            
            On Nov 23, 2018 this sequence version replaced NM_133494.2.
            
            Summary: NIMA-related kinases share high amino acid sequence
            identity with the gene product of the Aspergillus nidulans 'never
            in mitosis A' gene, which controls initiation of mitosis.[supplied
            by OMIM, Jul 2002].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.241144.1,
                                           SRR1163658.503772.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367385.9/ ENSP00000356355.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-497               DB498485.1         21-517
            498-1105            AK313411.1         318-925
            1106-4114           AC093419.2         112539-115547
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.3"
     gene            1..4114
                     /gene="NEK7"
                     /note="NIMA related kinase 7"
                     /db_xref="GeneID:140609"
                     /db_xref="HGNC:HGNC:13386"
                     /db_xref="MIM:606848"
     exon            1..279
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            280..364
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..1216
                     /gene="NEK7"
                     /EC_number="2.7.11.34"
                     /note="nimA-related protein kinase 7; never in mitosis
                     A-related kinase 7; NIMA (never in mitosis gene a)-related
                     kinase 7"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek7"
                     /protein_id="NP_598001.1"
                     /db_xref="CCDS:CCDS1394.1"
                     /db_xref="GeneID:140609"
                     /db_xref="HGNC:HGNC:13386"
                     /db_xref="MIM:606848"
                     /translation="MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQ
                     FSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFI
                     EDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHR
                     DIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSD
                     IWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCIN
                     PDPEKRPDVTYVYDVAKRMHACTASS"
     exon            365..505
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            506..568
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            569..679
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            680..788
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            789..896
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            897..991
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1105
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..4114
                     /gene="NEK7"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4086
                     /gene="NEK7"
                     /note="major polyA site"
     regulatory      4093..4098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK7"
                     /note="hexamer: AATAAA"
     polyA_site      4114
                     /gene="NEK7"
                     /note="major polyA site"
ORIGIN      
        1 acactcctga cagcggcggc agcaggagga ggatcgggag tcgcgggagg atgggccgcc
       61 gctaggctcg cactccggac gcgcctcgca gtgcgcaggg tgggtgcccc gcgcctgcag
      121 cgtccgccgg ggcggcgcgg cgggaggtgg ccgacaggct ccgggcctcg cagcctcagc
      181 ccccggccca gcgcgctttc cgacggcggc gccgcgccga gccacccgcc cgcccaaggt
      241 ctctcgcggg cgggagaacg gaaaactccc aacttcctga gttctaaagt tcctgttgct
      301 tcagacaatg gatgagcaat cacaaggaat gcaagggcca cctgttcctc agttccaacc
      361 acagaaggcc ttacgaccgg atatgggcta taatacatta gccaactttc gaatagaaaa
      421 gaaaattggt cgcggacaat ttagtgaagt ttatagagca gcctgtctct tggatggagt
      481 accagtagct ttaaaaaaag tgcagatatt tgatttaatg gatgccaaag cacgtgctga
      541 ttgcatcaaa gaaatagatc ttcttaagca actcaaccat ccaaatgtaa taaaatatta
      601 tgcatcattc attgaagata atgaactaaa catagttttg gaactagcag atgctggcga
      661 cctatccaga atgatcaagc attttaagaa gcaaaagagg ctaattcctg aaagaactgt
      721 ttggaagtat tttgttcagc tttgcagtgc attggaacac atgcattctc gaagagtcat
      781 gcatagagat ataaaaccag ctaatgtgtt cattacagcc actggggtgg taaaacttgg
      841 agatcttggg cttggccggt ttttcagctc aaaaaccaca gctgcacatt ctttagttgg
      901 tacgccttat tacatgtctc cagagagaat acatgaaaat ggatacaact tcaaatctga
      961 catctggtct cttggctgtc tactatatga gatggctgca ttacaaagtc ctttctatgg
     1021 tgacaaaatg aatttatact cactgtgtaa gaagatagaa cagtgtgact acccacctct
     1081 tccttcagat cactattcag aagaactccg acagttagtt aatatgtgca tcaacccaga
     1141 tccagagaag cgaccagacg tcacctatgt ttatgacgta gcaaagagga tgcatgcatg
     1201 cactgcaagc agctaaacat gcaagatcat gaagagtgta accaaagtaa ttgaaagtat
     1261 tttgtgcaag tcatacctcc ccatttatgt ctggtgttaa gattaatatt tcagagctag
     1321 tgtgctttga atccttaacc agttttcata taagcttcat tttgtaccag tcacctaaat
     1381 cacctccttg caacccccaa atgactttgg aataactgaa ttgcatgtta ggagagaaaa
     1441 tgaaacatga tggttttgaa tggctaaagg tttatagaat ttcttacagt tttctgctga
     1501 taaattgtgt ttagatagac tgtcagtgcc aaatattgaa ggtgcagctt ggcacacatc
     1561 agaatagact catacctgag aaaaagtatc tgaacatgtg acttgtttct tttttagtaa
     1621 tttatggaca ttgagatgaa cacaattgtg aacttttgtg aagattttat ttttaaacgt
     1681 ttgaagtact agttttagtt cttagcagag tagttttcaa atatgattct tatgataaat
     1741 gtagacacaa actatttgag aaacatttag aactcttagc ttatacattc aaaatgtaac
     1801 tattaaatgt gaagatttgg ggacaaaatg tgagtcagac actgaagagt tttttgtttt
     1861 gttttaatat ttttgatatt ctctttgcat tgaaatggta taaatgaatc catttaaaaa
     1921 gtggttaagg atttgtttag ctggtgtgat aataattttt aaagttgcac attgcccaag
     1981 gctttttttg tgtgttttta ttgttgtttg tacatttgaa aaatattctt tgaataacct
     2041 tgcagtacta tatttcaatt tctttataaa tttaagtgca ttttaactca taattgtaca
     2101 ctataatata agcctaagtt tttattcata agttttattg aagttctgat cggtcccctt
     2161 cagaaatttt tttatattat tcttcaagtt actttcttat ttatattgta tgtgcatttt
     2221 atccattaat gtttcatact ttctgagagt ataataccct tttaaaagat atttggtata
     2281 ccaatacttt tcctggattg aaaacttttt ttaaactttt taaaatttgg gccactctgt
     2341 atgcatatgt ttggtcttgt taaagaggaa gaaaggatgt gtgttatact gtacctgtga
     2401 atgttgatac agttacaatt tatttgacaa ggttgtaatt ctagaatatg cttaataaaa
     2461 tgaaaactgg ccatgactac agccagaact gttatgagat taacatttct attgagaagc
     2521 ttttgagtaa agtactgtat ttgttcatga agatgactga gatggtaaca cttcgtgtag
     2581 cttaaggaaa tgggcagaat ttcgtaaatg ctgttgtgca gatgtgtttt ccctgaatgc
     2641 tttcgtatta gtggcgacca gtttctcaca gaattgtgaa gcctgaaggc caagaggaag
     2701 tcactgttaa aggactctgt gccatcttac aaccttggat gaattatcct gccaacgtga
     2761 aaacctcatg ttcaaagaac acttcccttt agccgatgta actgctggtt ttgtttttca
     2821 tatgtgtttt tcttacactc atttgaatgc tttcaagcat ttgtaaactt aaaaaatgta
     2881 taaagggcaa aaagtctgaa cccttgtttt ctgaaatcta atcagttatg tatggtttct
     2941 gaagggtaat tttattttgg aataggtaaa ggaaacctgt tttgtttgtt tttcctgagg
     3001 gctagatgca ttttttttct cacactctta atgactttta acatttatac tgagcatcca
     3061 tagatatatt cctagaagta tgagaagaat tattcttatt gaccattaat gtcatgttca
     3121 ttttaatgta atataattga gatgaaatgt tctctggttg gaacagatac tctctttttt
     3181 ttcttgcaat ctttaagaat acatagatct aaaattcatt agcttgaccc ctcaaagtaa
     3241 cttttaagta aagattaaag cttttcttct cagtgaatat atctgctaga aggaaatagc
     3301 tgggaagaat ttaatgatca gggaaattca ttatttctat atgtggaaac tttttgcttc
     3361 gaatattgta tctttttaaa tctaaatgtt catatttttc ctgaagaaac cactgtgtaa
     3421 aaatcaaatt ttaattttga atggaataat ttcaaagaac tatgaagatg atttgaagct
     3481 ctaatttata tagtcaccta taaaatgttc tttatatgtg ttcataagta aattttatat
     3541 tgattaagtt aaacttttga attgatttga ggagcagtaa aatgaaagct atatctattc
     3601 taaaccttat ttagacattg gtaccagtta cccaggtgaa aatatggagt aactttgttt
     3661 tgtatggtaa ggtttaggaa tggtggatga agggtatctc tatataaata aagtgctcaa
     3721 caatgtgcaa tgattgtaaa tttagtaaga tattacagcc atttcatgaa tgctttacca
     3781 ttcaacatag tatctattac aaaacacctt tcttgtatcc atatacttca ggtgttgctg
     3841 ttaacattta ctatgatatt tattttaacc aaaatgttac tcacattaaa tgtttattct
     3901 ttaaaatgaa tgtattatgt ttttaaccca caaatgcata cttaccctgt gcctcatatt
     3961 tcaatagtac tgtaatatgg acatcttttg tgaaatactt ttattttgtt atgctttaaa
     4021 tatacataca aaaagatttc tgttattagc tttgaaaatt gtataatatc ctaatataaa
     4081 caaaaatata aaaataaaaa tgaatacagt aaaa
//
