LOCUS       NM_014397               3468 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens NIMA related kinase 6 (NEK6), transcript variant 2,
            mRNA.
ACCESSION   NM_014397
VERSION     NM_014397.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Guo,W., Wang,H., Kumar Tharkeshwar,A., Couthouis,J., Braems,E.,
            Masrori,P., Van Schoor,E., Fan,Y., Ahuja,K., Moisse,M.,
            Jacquemyn,M., Furtado Madeiro da Costa,R., Gajjar,M., Balusu,S.,
            Tricot,T., Fumagalli,L., Hersmus,N., Janky,R., Impens,F., Vanden
            Berghe,P., Ho,R., Thal,D.R., Vandenberghe,R., Hegde,M.L.,
            Chandran,S., De Strooper,B., Daelemans,D., Van Damme,P., Van Den
            Bosch,L. and Verfaillie,C.
  TITLE     CRISPR/Cas9 screen in human iPSC-derived cortical neurons
            identifies NEK6 as a novel disease modifier of C9orf72 poly(PR)
            toxicity
  JOURNAL   Alzheimers Dement 19 (4), 1245-1259 (2023)
   PUBMED   35993441
  REMARK    GeneRIF: CRISPR/Cas9 screen in human iPSC-derived cortical neurons
            identifies NEK6 as a novel disease modifier of C9orf72 poly(PR)
            toxicity.
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Pavan,I.C.B., Basei,F.L., Severino,M.B., Rosa E Silva,I.,
            Issayama,L.K., Mancini,M.C.S., Gois,M.M., da Silva,L.G.S.,
            Bezerra,R.M.N., Simabuco,F.M. and Kobarg,J.
  TITLE     NEK6 Regulates Redox Balance and DNA Damage Response in DU-145
            Prostate Cancer Cells
  JOURNAL   Cells 12 (2), 256 (2023)
   PUBMED   36672191
  REMARK    GeneRIF: NEK6 Regulates Redox Balance and DNA Damage Response in
            DU-145 Prostate Cancer Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3468)
  AUTHORS   Yang,Z.M., Liao,B., Yang,S.S., Su,T., Zhang,J. and Wang,W.M.
  TITLE     Predictive Role of NEK6 in Prognosis and Immune Infiltration in
            Head and Neck Squamous Cell Carcinoma
  JOURNAL   Front Endocrinol (Lausanne) 13, 943686 (2022)
   PUBMED   35898455
  REMARK    GeneRIF: Predictive Role of NEK6 in Prognosis and Immune
            Infiltration in Head and Neck Squamous Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3468)
  AUTHORS   Yu,Y., Shen,T., Zhong,X., Wang,L.L., Tai,W., Zou,Y., Qin,J.,
            Zhang,Z. and Zhang,C.L.
  TITLE     NEK6 is an injury-responsive kinase cooperating with STAT3 in
            regulation of reactive astrogliosis
  JOURNAL   Glia 70 (2), 273-286 (2022)
   PUBMED   34643969
  REMARK    GeneRIF: NEK6 is an injury-responsive kinase cooperating with STAT3
            in regulation of reactive astrogliosis.
REFERENCE   5  (bases 1 to 3468)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3468)
  AUTHORS   Belham,C., Comb,M.J. and Avruch,J.
  TITLE     Identification of the NIMA family kinases NEK6/7 as regulators of
            the p70 ribosomal S6 kinase
  JOURNAL   Curr Biol 11 (15), 1155-1167 (2001)
   PUBMED   11516946
REFERENCE   7  (bases 1 to 3468)
  AUTHORS   Kimura,M. and Okano,Y.
  TITLE     Identification and assignment of the human NIMA-related protein
            kinase 7 gene (NEK7) to human chromosome 1q31.3
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 33-38 (2001)
   PUBMED   11701951
  REMARK    GeneRIF: Also includes phylogenetic analysis and tissue-specific
            RT-PCR of human NEK6 and NEK7.
REFERENCE   8  (bases 1 to 3468)
  AUTHORS   Kandli,M., Feige,E., Chen,A., Kilfin,G. and Motro,B.
  TITLE     Isolation and characterization of two evolutionarily conserved
            murine kinases (Nek6 and nek7) related to the fungal mitotic
            regulator, NIMA
  JOURNAL   Genomics 68 (2), 187-196 (2000)
   PUBMED   10964517
REFERENCE   9  (bases 1 to 3468)
  AUTHORS   Kobayashi,T. and Cohen,P.
  TITLE     Activation of serum- and glucocorticoid-regulated protein kinase by
            agonists that activate phosphatidylinositide 3-kinase is mediated
            by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2
  JOURNAL   Biochem J 339 (Pt 2) (Pt 2), 319-328 (1999)
   PUBMED   10191262
REFERENCE   10 (bases 1 to 3468)
  AUTHORS   Li,M.Z., Yu,L., Liu,Q., Chu,J.Y. and Zhao,S.Y.
  TITLE     Assignment of NEK6, a NIMA-related gene, to human chromosome 9q33.
            3-->q34.11 by radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 87 (3-4), 271-272 (1999)
   PUBMED   10702691
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CF994196.1, BC012761.2,
            AL137846.24 and AF087909.1.
            
            On Feb 27, 2020 this sequence version replaced NM_014397.5.
            
            Summary: The protein encoded by this gene is a kinase required for
            progression through the metaphase portion of mitosis. Inhibition of
            the encoded protein can lead to apoptosis. This protein also can
            enhance tumorigenesis by suppressing tumor cell senescence. Several
            transcript variants encoding a few different isoforms have been
            found for this gene. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) is shorter than isoform 1. Variants 2, 5 and 6 encode the same
            isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.27793.1,
                                           SRR11853567.30839.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000320246.10/ ENSP00000319734.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                CF994196.1         60-127
            69-862              BC012761.2         48-841
            863-863             AL137846.24        13825-13825
            864-1541            BC012761.2         843-1520
            1542-1542           AF087909.1         1510-1510
            1543-2603           BC012761.2         1522-2582
            2604-3468           AL137846.24        26623-27487
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..3468
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /note="NIMA related kinase 6"
                     /db_xref="GeneID:10783"
                     /db_xref="HGNC:HGNC:7749"
                     /db_xref="MIM:604884"
     exon            1..137
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            138..256
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1108
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /EC_number="2.7.11.34"
                     /note="isoform 2 is encoded by transcript variant 2;
                     putative serine-threonine protein kinase;
                     serine/threonine-protein kinase Nek6; protein kinase
                     SID6-1512; nimA-related protein kinase 6; never in mitosis
                     A-related kinase 6; NIMA (never in mitosis gene a)-related
                     kinase 6"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek6 isoform 2"
                     /protein_id="NP_055212.2"
                     /db_xref="CCDS:CCDS6854.1"
                     /db_xref="GeneID:10783"
                     /db_xref="HGNC:HGNC:7749"
                     /db_xref="MIM:604884"
                     /translation="MAGQPGHMPHGGSSNNLCHTLGPVHPPDPQRHPNTLSFRCSLAD
                     FQIEKKIGRGQFSEVYKATCLLDRKTVALKKVQIFEMMDAKARQDCVKEIGLLKQLNH
                     PNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQKRLIPERTVWKYFVQLCSAV
                     EHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSETTAAHSLVGTPYYMSPER
                     IHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSE
                     KLRELVSMCICPDPHQRPDIGYVHQVAKQMHIWMSST"
     exon            257..397
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            398..460
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            461..571
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            572..680
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            681..788
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            789..883
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            884..997
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     exon            998..3468
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1590..1595
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /note="hexamer: GATAAA"
     polyA_site      1619
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /note="major polyA site"
     regulatory      2554..2559
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /note="hexamer: AATAAA"
     polyA_site      2574
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
     polyA_site      2603
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
     polyA_site      2649
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
     regulatory      3148..3153
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
                     /note="hexamer: ATTACA"
     polyA_site      3170
                     /gene="NEK6"
                     /gene_synonym="SID6-1512"
ORIGIN      
        1 gcggtggcgg cggcggcgga accgagctga cgggcgtgcg gccgctgcgc cgcaaactcg
       61 tgtgggacgc accgctccag ccgcccgcgg gccagcgcac cggtccccca gcggcagccg
      121 agcccgcccg cgcgccgttc gtgccctcgt gaggctggca tgcaggatgg caggacagcc
      181 cggccacatg ccccatggag ggagttccaa caacctctgc cacaccctgg ggcctgtgca
      241 tcctcctgac ccacagaggc atcccaacac gctgtctttt cgctgctcgc tggcggactt
      301 ccagatcgaa aagaagatag gccgaggaca gttcagcgag gtgtacaagg ccacctgcct
      361 gctggacagg aagacagtgg ctctgaagaa ggtgcagatc tttgagatga tggacgccaa
      421 ggcgaggcag gactgtgtca aggagatcgg cctcttgaag caactgaacc acccaaatat
      481 catcaagtat ttggactcgt ttatcgaaga caacgagctg aacattgtgc tggagttggc
      541 tgacgcaggg gacctctcgc agatgatcaa gtactttaag aagcagaagc ggctcatccc
      601 ggagaggaca gtatggaagt actttgtgca gctgtgcagc gccgtggagc acatgcattc
      661 acgccgggtg atgcaccgag acatcaagcc tgccaacgtg ttcatcacag ccacgggcgt
      721 cgtgaagctc ggtgaccttg gtctgggccg cttcttcagc tctgagacca ccgcagccca
      781 ctccctagtg gggacgccct actacatgtc accggagagg atccatgaga acggctacaa
      841 cttcaagtcc gacatctggt ccctgggctg tctgctgtac gagatggcag ccctccagag
      901 ccccttctat ggagataaga tgaatctctt ctccctgtgc cagaagatcg agcagtgtga
      961 ctacccccca ctccccgggg agcactactc cgagaagtta cgagaactgg tcagcatgtg
     1021 catctgccct gacccccacc agagacctga catcggatac gtgcaccagg tggccaagca
     1081 gatgcacatc tggatgtcca gcacctgagc gtggatgcac cgtgccttat caaagccagc
     1141 accactttgc cttacttgag tcgtcttctc ttcgagtggc cacctggtag cctagaacag
     1201 ctaagaccac agggttcagc aggttcccca aaaggctgcc cagccttaca gcagatgctg
     1261 aaggcagagc agctgaggga ggggcgctgg ccacatgtca ctgatggtca gattccaaag
     1321 tcctttcttt atactgttgt ggacaatctc agctgggtca ataagggcag gtggttcagc
     1381 gagccacggc agccccctgt atctggattg taatgtgaat ctttagggta attcctccag
     1441 tgacctgtca aggcttatgc taacaggaga cttgcaggag accgtgtgat ttgtgtagtg
     1501 agcctttgaa aatggttagt accgggttca gtttagttct tagtatcttt tcaatcaagc
     1561 tgtgtgctta atttactctg ttgtaaaggg ataaagtgga aatcattttt ttccgtggag
     1621 tggtgattct gctaacattt ttatctacgt tttataactt ggtgagtgac gatgagagcc
     1681 ctgcacctgg ccagagtgtc acaggcaaaa ggcatcggga agcaggagca tcttcttggc
     1741 agccaggctg ggccatcttc tcctggacac ctgctgtgta ccaggaactt cgtcacctcc
     1801 ttgaatgctg gcggttcatt tcatgatcag tgttaagcat tttcctccat gggaaggaag
     1861 catgggatat agaaaagcga agggctgtcc tttacaaatt ctggttctgc aacttcctag
     1921 cgtgactttg ggcttgggca agtttcttag ccgttctgag ccttcatttc ctcatctgta
     1981 caatgagatt aatagtacct atcatctacc ttcaggattg ctgacagaca gaatttgaaa
     2041 taaaatatgc aagttagcta atacaaaaag tagatgatcc aaaaatggta gccactcacc
     2101 cttcacaaac tgaagtccat ggaccacgga agtcgagaat taatgtacac ctgtatcatg
     2161 tgtaggaaac cagaaatgtg ttccttattt cttgttccca aacaggatta actgtgaaga
     2221 ctaatttata aatgtgaacc taagaaaact ccacctctga aggaaatcat ttgaattttg
     2281 tttttgtacg taaagttaac cttccaattg tctgagctgt cgtcactgac ttcatgacag
     2341 tctggccctc cagacaagag cagcgctggc atcgggcagg tgattcctga cacctgctgc
     2401 ctgcaggcat tcactgacca ggcctttcct ggaggaaaca cccagggccg ggcggctgct
     2461 gtttccacac gtggactcgg atctgctgtg acaccgtcag cccgacagtc tctccatatg
     2521 cagcctttcc tctgtacttt tctccatggt tgaaataaaa cagggtgact gggagttact
     2581 tagaattcat gaagatttta aaatggcttt ggagattttg cttttaaacc agtagattca
     2641 aaacttaaac agcgtctgca gcacaatttc ttgaggaacc ttgaaaaaca caacttccca
     2701 ggccccattc agtaatccca ggatttcttt aagctcccca aataattttg aaactcatca
     2761 tcagccgagt ttctgccctc atgaggtaat tccatcgttc tccccagcct gcccctggca
     2821 gctgtaacac aggagctggc ctgagagcag attcaccctg gaatgttctc tccacagaac
     2881 aatcaagtcc ctgtcgcctg cctagtgctt accactgaag atttttctga ttccagacca
     2941 actttttgcc aacattctgc ttccagctct ctgagcccct gccgtgtctc cccaacactg
     3001 ccagccccag cacgcaatca acctactttg tgcatgccac ccgctttcca cactgtgaga
     3061 acaatctgcc caactggacc ctctggaggc gcacgatctc agccactcac caggcctgag
     3121 tgtttgtgaa atgatcatgt cctacttatt acaaaaccgt aaccccaaaa cattcctttt
     3181 atttctgtga aaccggccaa agtgaggtcc acccaccttc acacagctct ggcggtgcac
     3241 ctgctcacct tctcttggtt ctcagaactg agctgggctt gagaacacag ctttggcttt
     3301 gccatttttt tcctacttgg ctgctgaggt ggagggtgtg ctgcacttat caccccattt
     3361 caaaaccaaa ccaaacctga ggccacccca aacaaattca gccagcaaaa agggtaggta
     3421 tcgatggtca cctgaagcct caagggagtc cactctgact tctgacag
//
