LOCUS       NM_003157               6053 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens NIMA related kinase 4 (NEK4), transcript variant 1,
            mRNA.
ACCESSION   NM_003157
VERSION     NM_003157.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Zhang,C., Yang,Z., Li,X., Zhao,L., Guo,W., Deng,W., Wang,Q., Hu,X.,
            Li,M., Sham,P.C., Xiao,X. and Li,T.
  TITLE     Unraveling NEK4 as a Potential Drug Target in Schizophrenia and
            Bipolar I Disorder: A Proteomic and Genomic Approach
  JOURNAL   Schizophr Bull 50 (5), 1185-1196 (2024)
   PUBMED   38869147
  REMARK    GeneRIF: Unraveling NEK4 as a Potential Drug Target in
            Schizophrenia and Bipolar I Disorder: A Proteomic and Genomic
            Approach.
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Ahmad,F., Tomar,D., Aryal A,C. S, Elmoselhi,A.B., Thomas,M.,
            Elrod,J.W., Tilley,D.G. and Force,T.
  TITLE     Nicotinamide riboside kinase-2 alleviates ischemia-induced heart
            failure through P38 signaling
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (3), 165609 (2020)
   PUBMED   31743747
  REMARK    GeneRIF: NRK-2 deficiency promotes post-MI scar expansion, rapid LV
            chamber dilatation, cardiac dysfunction and fibrosis possibly due
            to increased p38alpha activation.
REFERENCE   3  (bases 1 to 6053)
  AUTHORS   Ding,N.H., Zhang,L., Xiao,Z., Rong,Z.X., Li,Z., He,J., Chen,L.,
            Ou,D.M., Liao,W.H. and Sun,L.Q.
  TITLE     NEK4 kinase regulates EMT to promote lung cancer metastasis
  JOURNAL   J Cell Mol Med 22 (12), 5877-5887 (2018)
   PUBMED   30247800
  REMARK    GeneRIF: this study demonstrates NEK4 as a novel kinase involved in
            regulation of Epithelial-to-mesenchymal transition.
REFERENCE   4  (bases 1 to 6053)
  AUTHORS   Shaheen,R., Szymanska,K., Basu,B., Patel,N., Ewida,N., Faqeih,E.,
            Al Hashem,A., Derar,N., Alsharif,H., Aldahmesh,M.A., Alazami,A.M.,
            Hashem,M., Ibrahim,N., Abdulwahab,F.M., Sonbul,R., Alkuraya,H.,
            Alnemer,M., Al Tala,S., Al-Husain,M., Morsy,H., Seidahmed,M.Z.,
            Meriki,N., Al-Owain,M., AlShahwan,S., Tabarki,B., Salih,M.A.,
            Faquih,T., El-Kalioby,M., Ueffing,M., Boldt,K., Logan,C.V.,
            Parry,D.A., Al Tassan,N., Monies,D., Megarbane,A., Abouelhoda,M.,
            Halees,A., Johnson,C.A. and Alkuraya,F.S.
  CONSRTM   Ciliopathy WorkingGroup
  TITLE     Characterizing the morbid genome of ciliopathies
  JOURNAL   Genome Biol 17 (1), 242 (2016)
   PUBMED   27894351
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6053)
  AUTHORS   Basei,F.L., Meirelles,G.V., Righetto,G.L., Dos Santos
            Migueleti,D.L., Smetana,J.H. and Kobarg,J.
  TITLE     New interaction partners for Nek4.1 and Nek4.2 isoforms: from the
            DNA damage response to RNA splicing
  JOURNAL   Proteome Sci 13, 11 (2015)
   PUBMED   25798074
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6053)
  AUTHORS   Nguyen,C.L., Possemato,R., Bauerlein,E.L., Xie,A., Scully,R. and
            Hahn,W.C.
  TITLE     Nek4 regulates entry into replicative senescence and the response
            to DNA damage in human fibroblasts
  JOURNAL   Mol Cell Biol 32 (19), 3963-3977 (2012)
   PUBMED   22851694
  REMARK    GeneRIF: Nek4 as a novel regulator of replicative senescence and
            the response to double-stranded DNA damage.
REFERENCE   7  (bases 1 to 6053)
  AUTHORS   Coene,K.L., Mans,D.A., Boldt,K., Gloeckner,C.J., van Reeuwijk,J.,
            Bolat,E., Roosing,S., Letteboer,S.J., Peters,T.A., Cremers,F.P.,
            Ueffing,M. and Roepman,R.
  TITLE     The ciliopathy-associated protein homologs RPGRIP1 and RPGRIP1L are
            linked to cilium integrity through interaction with Nek4
            serine/threonine kinase
  JOURNAL   Hum Mol Genet 20 (18), 3592-3605 (2011)
   PUBMED   21685204
  REMARK    GeneRIF: Nek4 interaction with both RPGRIP1 and the RPGRIP1L is
            involved in cilium assembly.
REFERENCE   8  (bases 1 to 6053)
  AUTHORS   Doles,J. and Hemann,M.T.
  TITLE     Nek4 status differentially alters sensitivity to distinct
            microtubule poisons
  JOURNAL   Cancer Res 70 (3), 1033-1041 (2010)
   PUBMED   20103636
  REMARK    GeneRIF: Data show that after Taxol treatment, Nek4 promoted
            microtubule outgrowth, whereas Nek4 deficiency impaired G(2)-M
            arrest and decreased formation of mitotic-like asters.
REFERENCE   9  (bases 1 to 6053)
  AUTHORS   Lu,K.P. and Hunter,T.
  TITLE     The NIMA kinase: a mitotic regulator in Aspergillus nidulans and
            vertebrate cells
  JOURNAL   Prog Cell Cycle Res 1, 187-205 (1995)
   PUBMED   9552363
  REMARK    Review article
REFERENCE   10 (bases 1 to 6053)
  AUTHORS   Levedakou,E.N., He,M., Baptist,E.W., Craven,R.J., Cance,W.G.,
            Welcsh,P.L., Simmons,A., Naylor,S.L., Leach,R.J., Lewis,T.B. et al.
  TITLE     Two novel human serine/threonine kinases with homologies to the
            cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and
            characterization of their expression pattern
  JOURNAL   Oncogene 9 (7), 1977-1988 (1994)
   PUBMED   8208544
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB073127.1, BC015515.1,
            L20321.1 and AC104446.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003157.5.
            
            Summary: The protein encoded by this gene is a serine/threonine
            protein kinase required for normal entry into replicative
            senescence. The encoded protein also is involved in cell cycle
            arrest in response to double-stranded DNA damage. Finally, this
            protein plays a role in maintaining cilium integrity, and defects
            in this gene have been associated with ciliopathies. [provided by
            RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L20321.1, SRR1660805.187757.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000233027.10/ ENSP00000233027.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-518               DB073127.1         10-527
            519-1535            BC015515.1         492-1508
            1536-3706           L20321.1           1520-3690
            3707-6053           AC104446.2         161209-163555       c
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.1"
     gene            1..6053
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /note="NIMA related kinase 4"
                     /db_xref="GeneID:6787"
                     /db_xref="HGNC:HGNC:11399"
                     /db_xref="MIM:601959"
     exon            1..287
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..2720
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine kinase 2; serine/threonine protein
                     kinase-2; nimA-related protein kinase 4; never in mitosis
                     A-related kinase 4; serine/threonine-protein kinase NRK2;
                     NIMA (never in mitosis gene a)-related kinase 4"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek4 isoform 1"
                     /protein_id="NP_003148.2"
                     /db_xref="CCDS:CCDS2863.1"
                     /db_xref="GeneID:6787"
                     /db_xref="HGNC:HGNC:11399"
                     /db_xref="MIM:601959"
                     /translation="MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASS
                     RERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQL
                     LPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHC
                     DMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRI
                     IEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKT
                     SKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRA
                     SGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLD
                     ASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKE
                     QKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGE
                     CIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPR
                     FLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQS
                     QEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELS
                     SSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHR
                     KYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQV
                     YDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF"
     exon            288..554
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            555..752
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            753..860
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1015
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1157
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1562
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1700
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1871
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2021
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2088
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2198
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2287
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2493
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2627
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2628..6053
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3694..3699
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /note="hexamer: AATAAA"
     polyA_site      3709
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /note="major polyA site"
     polyA_site      3713
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /note="major polyA site"
     regulatory      5799..5804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /note="hexamer: AATAAA"
     polyA_site      5825
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
     regulatory      6029..6034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
                     /note="hexamer: AATAAA"
     polyA_site      6053
                     /gene="NEK4"
                     /gene_synonym="NRK2; pp12301; STK2"
ORIGIN      
        1 agaactgctc ccggcccgga tcgctatggc agcggcgtcg tcgcgggccg ggccccagca
       61 atcccgcccg ggcccggctg cctcaacagc cgcccccact gccccctctc gggcatgaac
      121 cgagcttctt gttgccgccc gctgccctac ccgccgctgc cgccgcatcc cgactctggg
      181 ccagcgctgg gaacatgccc ctggccgcct actgctacct gcgggtcgtg ggcaagggga
      241 gctatggaga ggtgacgctt gtgaagcacc ggcgggacgg caagcagtat gtcatcaaaa
      301 aactgaacct ccgaaatgcc tctagccgag agcggcgagc tgctgaacag gaagcccagc
      361 tcttgtctca gttgaagcat cccaacattg tcacctacaa ggagtcatgg gaaggaggag
      421 atggtctgct ctacattgtc atgggcttct gtgaaggagg tgatttgtac cgaaagctca
      481 aggagcagaa agggcagctt ctgcctgaga atcaggtggt agagtggttt gtacagatcg
      541 ccatggcttt gcagtattta catgaaaaac acatccttca tcgagatctg aaaactcaaa
      601 atgtcttcct aacaagaaca aacatcatca aagtagggga cctaggaatt gcccgagtgt
      661 tagagaacca ctgtgacatg gctagcaccc tcattggcac accctactac atgagccctg
      721 aattgttctc aaacaaaccc tacaactata agtctgatgt ttgggctcta ggatgctgtg
      781 tctatgaaat ggccaccttg aagcatgctt tcaatgcaaa agatatgaat tctttagttt
      841 atcggattat tgaaggaaag ctgccaccaa tgccaagaga ttacagccca gagctggcag
      901 aactgataag aacaatgctg agcaaaaggc ctgaagaaag gccgtctgtg aggagcatcc
      961 tgaggcagcc ttatataaag cggcaaatct ccttcttttt ggaggccaca aagataaaaa
     1021 cctccaaaaa taacattaaa aatggtgact ctcaatccaa gccttttgct acagtggttt
     1081 ctggagaggc agaatcaaat catgaagtaa tccaccccca accactctct tctgagggct
     1141 cccagacata tataatgggt gaaggcaaat gtttgtccca ggagaaaccc agggcctctg
     1201 gtctcttgaa gtcacctgcc agtctgaaag cccatacctg caaacaggac ttgagcaata
     1261 ccacagaact agccacaatc agtagcgtaa atattgacat cttacctgca aaagggaggg
     1321 attcagtgag tgatggcttt gttcaggaga atcagccaag atatttggat gcctctaatg
     1381 agttaggagg tatatgcagt atttctcaag tggaagagga gatgctgcag gacaacacta
     1441 aatccagtgc ccagcctgaa aacctgattc ccatgtggtc ctctgacatt gtcactgggg
     1501 aaaagaatga accagtgaag cctctgcagc ccctaatcaa agaacaaaag ccaaaggacc
     1561 agagtcttgc cctgtcgccc aagctggagt gcagtggcac aatcttggct cacagcaacc
     1621 tccgcctcct gggttcaagt gattctccag cctcagcctc ccgagtagct gggattacag
     1681 gcgtgtgcca ccacgcccag gatcaagttg ctggtgaatg tattatagaa aaacagggca
     1741 gaatccaccc agatttacag ccacacaact ctgggtctga accttccctg tctcgacagc
     1801 gacggcaaaa gaggagagaa cagactgagc acagagggga aaagagacag gtccgcagag
     1861 atctctttgc tttccaagag tcgcctcctc gatttttgcc ttctcatccc attgttggga
     1921 aagtggatgt cacatcaaca caaaaagagg ctgaaaacca acgtagagtg gtcactgggt
     1981 ctgtgagcag ttcaaggagc agtgagatgt catcatcaaa ggatcgacca ttatcagcca
     2041 gagagaggag gcgactaaag cagtcacagg aagaaatgtc ctcttcaggc ccttcagtga
     2101 ggaaagcgtc tctgagtgta gcagggccag gaaaacccca ggaagaagac cagcccttgc
     2161 ctgcccgacg gctctcctct gactgcagcg tcactcagga aaggaaacag attcattgtc
     2221 tgtctgagga tgagttaagt tcttctacaa gttcaactga taagtcagat ggggattacg
     2281 gggaagggaa aggtcagaca aatgaaatta atgccttggt acaattgatg actcagaccc
     2341 tgaaactgga ttctaaagag agctgtgaag atgtcccggt agcaaaccca gtgtcagaat
     2401 tcaaacttca tcggaaatat cgggacacac tgatacttca tgggaaggtt gcagaagagg
     2461 cagaggaaat ccattttaaa gagctacctt cagctattat gccaggttct gaaaagatca
     2521 ggagactagt tgaagtcttg agaactgatg taattcgtgg cctgggagtt cagcttttag
     2581 agcaggtgta tgatcttttg gaggaggagg atgaatttga tagagaggta cgtttgcggg
     2641 agcacatggg tgaaaagtat acaacttaca gtgtgaaagc tcgccagttg aaattttttg
     2701 aagaaaacat gaatttttga gcatttgtcc taatctgctg ccagaattaa agacctattt
     2761 ttagaggatt ttggcttaaa aagcaagggc aaacagtcat ttggaagcca ctcaccactg
     2821 ttttatatct cttttttata tctctttggc gtttccctac agaaaagaaa ttggacagaa
     2881 cagaataata tgaagcagga tcacaaaaga aaaaaaactt tggctttcat attctctttg
     2941 tgaggacaaa tctgttgttt gtttgattac tgtttactga gccttaatcc accaagttta
     3001 tatttagaat tttatttttt taaggtacta attaacttaa acacagagct ataaaatgct
     3061 ggattgaaaa ttttatattg taatgtagag ataaaagcag taggagaaac aaatgacata
     3121 atatgtcgtc ataattcctg ctattgttaa taaccttaag gagtagttga taaattataa
     3181 aattttaaaa agtcaattca gttctagaaa tagatttaaa gaatatgaag ttctatctag
     3241 tacttgagca gctgtatttc ttttctacac attgatggac ttttaatatt ttattctcat
     3301 ttaatataaa cctcatctag ggtatataca aattaaaact gagacacatt ggctttgtaa
     3361 atcagtatgt ttttacataa tggttttgtt agatttattt ttccatcagt gaaaacattt
     3421 cttaaacaca aatttcattt ccatttaagc aatttgtaag caaagtccag gtccatttag
     3481 tttttggata tatttaatgt ttgtctcctg aagtttgtct tcatgtactg taagatatta
     3541 gttgtctttc catgttttaa atgtatgatt atatagcaca tattttatta gttgtttaat
     3601 aagaggtaat acccatctag gaaagaaatt ttatgaagtt aaatacaagt cttgaatagt
     3661 acattttcac ttctgtattc gagggactct aaaaataaat attgctccag aaatgttttc
     3721 aggtgttttt tttttgagac agagtttcac tctgtcagtc aggctggagt gcagtggcat
     3781 gatctcaact cactgcaacc tccacttctg gggctcaagt ggtattcctg cctgagcctc
     3841 cctagtagct gagaccacag gcgtgtgcca ccacacctgg ctagtttttg tattttttgt
     3901 agagacaggg ttacaccaca ttgcccaggc tgatctcaga actcctgggc tcaagcaatc
     3961 cactcacctc agcctcccaa agtgccagga ttacaggaat gagccactgt gcccagccct
     4021 gttttcagtt tctataacta aaaatatatc tatctttgaa aattacacac acacacaaat
     4081 actctaatga aatgttccct ttacagccac ttaacaattt tttttttttt ttaagacaca
     4141 gtcttgctct gtcacccagg ctgaagtgca gtggcccaat ctcagatcac tgcaagctcc
     4201 gcctcccagg ttcactccat tctcctacct catcctctcg agtagctggg actacagttg
     4261 cccgccacca tgcccggcta atttttttgt atttttttag tagagacagg gtttcaccgt
     4321 gttagccagg atggtctcaa tctcctgacc tcgtgatctg cccgcctcgg cctcccaaag
     4381 tgctgggatt acaggcgtga gccaccgcgc ccggcacact taacagattt ttatttagag
     4441 tttactgtgt gccaggttct ctgccaggag ctggggatat agtggtgaat aaaacaatac
     4501 ttcctgccct tttacagtgc tcacagcctg gtttctgata actcttattt tcactttgaa
     4561 gggtttgcta gaactcaact tgcaaaacca tcaagtccca atgcccagtt gggaacaaat
     4621 ctttggcaaa cttggtatcc ttgagctatt cacctttccg tgcatgtttt ggaaatccac
     4681 atcgttctgg agagtcatca gttttactta atttttctaa tgtattggta taaagtaggt
     4741 attttattag aattaaagga atctcaccca tatctttttt tttttttttt ttttttgaga
     4801 cagggtcttg ctctgtcacc cagcctggag tgcagtgagg tgatcatagc tcactgcagc
     4861 cttgacctcc tgggcttaag tgatcctccc acctcagccc ctcaagtagc taggaccaca
     4921 ggcatgcacc accacacctg gctaattttt aaaatttttt gtggagagga agtctgtgtt
     4981 gcccaggctg gtctcaaact gcattcaagc agtcctcctg catcggcctc tgaaagtgct
     5041 gggattatag gcatgaacca ccacacctga cttcccccat actttcttat taattaccat
     5101 actgtatatt tttattttgt ttaaacatct tttcttccca cttttatcgt ttttacttga
     5161 gtgcttaatt catcttccta aattaattta aaaactcatc tttaagactg tgaattttct
     5221 tccgaatata cctttggcca cagttcagag actttgatgt gttactcttt tggcattggt
     5281 tctaaaatgt caatcattct tttttttttt tttttgagat ggagtcttct tgttgcccag
     5341 gctggagtac agtggtgcga tcttggctca ctgcagccta cgcctcctgg gttcaagttc
     5401 ccctccctca gcctcccgag tagctgggat tacaggcaca caccaccatg cctggctgat
     5461 tttcgttatt tttagtagag atggagtttc accatgttgg ccaggctggt cttgaactcc
     5521 tgacctcagg tgatctgcct gccttggcct ccctaaatgc tgggattaca ggcttgaggc
     5581 accatgcctg gcctaaagtg tcagtaattc tgatttcggt tttctctttt agaagttttg
     5641 atttccacag acttacaaag ctaaggaagt tttatgtcct tgctctggct acactgcagt
     5701 gttactgaat atcacctgca caacctttac tgcttgcagt tttttttttc ctgttgcttt
     5761 gtacatgatc cgttattcaa atggccttat aggcattcaa taaaggtgaa atgttaaata
     5821 caaaatcact tgtctattaa attttattca aggttctata taagatctga taaatggaac
     5881 tttgtgatgg aaatgtttta tatggtagcc actagtcaca tatagctatt gaacctttga
     5941 atcatggcta gttttagagt tttatatttt aattttaatt aaatagacgt gtctagtggc
     6001 accatattgt acaatgcagt tctcattcaa taaatcttaa gtacttaaca atg
//
