LOCUS       NM_002498               2128 bp    mRNA    linear   PRI 17-JUL-2024
DEFINITION  Homo sapiens NIMA related kinase 3 (NEK3), transcript variant 1,
            mRNA.
ACCESSION   NM_002498
VERSION     NM_002498.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Melo-Hanchuk,T.D., Martins,M.B., Cunha,L.L., Soares,F.A.,
            Ward,L.S., Vassallo,J. and Kobarg,J.
  TITLE     Expression of the NEK family in normal and cancer tissue: an
            immunohistochemical study
  JOURNAL   BMC Cancer 20 (1), 23 (2020)
   PUBMED   31906878
  REMARK    GeneRIF: the NEK protein kinases emerge as important proteins in
            thyroid cancer development and may help to identify malignancy and
            aggressiveness features during diagnosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Rapaport,D., Fichtman,B., Weidberg,H., Sprecher,E. and Horowitz,M.
  TITLE     NEK3-mediated SNAP29 phosphorylation modulates its membrane
            association and SNARE fusion dependent processes
  JOURNAL   Biochem Biophys Res Commun 497 (2), 605-611 (2018)
   PUBMED   29454964
  REMARK    GeneRIF: NEK3 kinase phosphorylates SNAP29 on its serine 105.
            NEK3-mediated phosphorylation determines membrane localization of
            SNAP29. Membrane-associated SNAP29 regulates Golgi and focal
            adhesion structures.
REFERENCE   3  (bases 1 to 2128)
  AUTHORS   Cao,Y., Song,J., Chen,J., Xiao,J., Ni,J. and Wu,C.
  TITLE     Overexpression of NEK3 is associated with poor prognosis in
            patients with gastric cancer
  JOURNAL   Medicine (Baltimore) 97 (3), e9630 (2018)
   PUBMED   29504992
  REMARK    GeneRIF: The data demonstrate that NEK3 is overexpressed in gastric
            cancer, which promotes the malignancy of gastric cancer
REFERENCE   4  (bases 1 to 2128)
  AUTHORS   Harrington,K.M. and Clevenger,C.V.
  TITLE     Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory
            Phosphorylation Site That Modulates Focal Adhesion Remodeling
            Necessary for Breast Cancer Cell Migration
  JOURNAL   J Biol Chem 291 (41), 21388-21406 (2016)
   PUBMED   27489110
  REMARK    GeneRIF: these data support a modulatory role for phosphorylation
            at NEK3 Thr-165 in focal adhesion maturation and/or turnover to
            promote breast cancer cell migration.
REFERENCE   5  (bases 1 to 2128)
  AUTHORS   Benjamin,S., Weidberg,H., Rapaport,D., Pekar,O., Nudelman,M.,
            Segal,D., Hirschberg,K., Katzav,S., Ehrlich,M. and Horowitz,M.
  TITLE     EHD2 mediates trafficking from the plasma membrane by modulating
            Rac1 activity
  JOURNAL   Biochem J 439 (3), 433-442 (2011)
   PUBMED   21756249
  REMARK    GeneRIF: EHD2 interacts with Nek3 [NIMA (never in mitosis in
            Aspergillus nidulans)-related kinase 3], a serine/threonine kinase.
REFERENCE   6  (bases 1 to 2128)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   7  (bases 1 to 2128)
  AUTHORS   Kimura,M. and Okano,Y.
  TITLE     Molecular cloning and characterization of the human NIMA-related
            protein kinase 3 gene (NEK3)
  JOURNAL   Cytogenet Cell Genet 95 (3-4), 177-182 (2001)
   PUBMED   12063396
  REMARK    GeneRIF: Molecular cloning and characterization of the human
            NIMA-related protein kinase 3 gene (NEK3).
REFERENCE   8  (bases 1 to 2128)
  AUTHORS   Tanaka,K. and Nigg,E.A.
  TITLE     Cloning and characterization of the murine Nek3 protein kinase, a
            novel member of the NIMA family of putative cell cycle regulators
  JOURNAL   J Biol Chem 274 (19), 13491-13497 (1999)
   PUBMED   10224116
REFERENCE   9  (bases 1 to 2128)
  AUTHORS   Schultz,S.J., Fry,A.M., Sutterlin,C., Ried,T. and Nigg,E.A.
  TITLE     Cell cycle-dependent expression of Nek2, a novel human protein
            kinase related to the NIMA mitotic regulator of Aspergillus
            nidulans
  JOURNAL   Cell Growth Differ 5 (6), 625-635 (1994)
   PUBMED   7522034
REFERENCE   10 (bases 1 to 2128)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139082.18 and BC019916.1.
            
            On Nov 22, 2018 this sequence version replaced NM_002498.2.
            
            Summary: This gene encodes a member of the NimA (never in mitosis
            A) family of serine/threonine protein kinases. The encoded protein
            differs from other NimA family members in that it is not cell cycle
            regulated and is found primarily in the cytoplasm. The kinase is
            activated by prolactin stimulation, leading to phosphorylation of
            VAV2 guanine nucleotide exchange factor, paxillin, and activation
            of the RAC1 GTPase. Two functional alleles for this gene have been
            identified in humans. The reference genome assembly (GRCh38)
            represents a functional allele that is associated with the
            inclusion of an additional coding exon in protein-coding
            transcripts, compared to an alternate functional allele that lacks
            the exon. [provided by RefSeq, Sep 2019].
            
            Transcript Variant: This variant (1) represents the splice pattern
            of variant 4 but with the extra 'A' found in the GRCh38 haplotype.
            
            Sequence Note: This sequence contains an extra 'A' at the end of
            exon 10 that is found in the GRCh38 reference assembly
            (NC_000013.11). It encodes a full-length protein through inclusion
            of exon 11.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019916.1, SRR1803617.162474.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000610828.5/ ENSP00000480328.1
            polymorphic pseudogene :: based on alignments, homology
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                AL139082.18        178005-178059       c
            56-229              AL139082.18        174537-174710       c
            230-323             AL139082.18        172542-172635       c
            324-421             AL139082.18        172357-172454       c
            422-505             AL139082.18        171071-171154       c
            506-573             AL139082.18        169787-169854       c
            574-660             AL139082.18        169608-169694       c
            661-715             AL139082.18        166877-166931       c
            716-916             AL139082.18        163153-163353       c
            917-988             AL139082.18        162378-162449       c
            989-1039            BC019916.1         980-1030
            1040-1142           AL139082.18        155263-155365       c
            1143-1286           AL139082.18        154579-154722       c
            1287-1421           AL139082.18        154192-154326       c
            1422-1548           AL139082.18        152152-152278       c
            1549-2128           AL139082.18        151110-151689       c
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.3"
     gene            1..2128
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /note="NIMA related kinase 3"
                     /db_xref="GeneID:4752"
                     /db_xref="HGNC:HGNC:7746"
                     /db_xref="MIM:604044"
     exon            1..55
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            56..229
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..1633
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1;
                     serine/threonine-protein kinase NEK3; phosphorylase B
                     kinase kinase; glycogen synthase A kinase;
                     hydroxyalkyl-protein kinase; HSPK 36; nimA-related protein
                     kinase 3; never in mitosis A-related kinase 3; NIMA (never
                     in mitosis gene a)-related kinase 3"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek3 isoform a"
                     /protein_id="NP_002489.1"
                     /db_xref="CCDS:CCDS73576.1"
                     /db_xref="GeneID:4752"
                     /db_xref="HGNC:HGNC:7746"
                     /db_xref="MIM:604044"
                     /translation="MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNT
                     QNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPED
                     MILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFAC
                     TYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGC
                     ISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEE
                     VLEEIKNSKHNTPRKKTNPSRIRIALGNEASTVQEEEQDRKGSHTDLESINENLVESA
                     LRRVNREEKGNKSVHLRKASSPNLHRRQWEKNVPNTALTALENASILTSSLTAEDDRG
                     GSVIKYSKNTTRKQWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEET
                     EASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR"
     exon            230..323
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            324..421
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            422..505
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            506..573
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            574..660
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            661..715
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            716..916
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            917..989
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1039
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1142
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1286
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1421
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1548
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..2128
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2108..2113
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /note="hexamer: ATTAAA"
     polyA_site      2128
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /note="major polyA site"
ORIGIN      
        1 gcggaagccg ccggttgcca ggccaaggag tggactaggg tcgccgggga agcggtttgg
       61 gagagcccat ggtgactgcg tgagtggagc ccagctgtgt ggatgcccca gcatggatga
      121 ctacatggtc ctgagaatga ttggggaggg ctccttcggc agagctcttt tggttcagca
      181 tgaaagcagt aatcagatgt ttgccatgaa agaaataagg cttcccaagt ctttctctaa
      241 tacacagaat tctaggaagg aggctgttct tttagccaaa atgaaacacc ctaatattgt
      301 tgccttcaaa gaatcatttg aagctgaagg acacttgtat attgtgatgg aatactgtga
      361 tggaggggat ctaatgcaaa agattaaaca gcagaaagga aagttatttc ctgaagacat
      421 gatacttaat tggtttaccc aaatgtgcct tggagtaaat cacattcaca agaaacgtgt
      481 gctacacaga gatatcaagt ccaagaatat cttcctcact cagaatggaa aagtgaaatt
      541 gggagacttt ggatctgccc gtcttctctc caatccgatg gcatttgctt gtacctatgt
      601 gggaactcct tattatgtgc ctccagaaat ttgggaaaac ctgccttata acaataaaag
      661 tgacatctgg tccttgggtt gcatcctgta tgaactctgt acccttaagc atccatttca
      721 ggcaaatagt tggaaaaatc ttatcctcaa agtatgtcaa gggtgcatca gtccactgcc
      781 gtctcattac tcctatgaac ttcagttcct agtcaagcag atgtttaaaa ggaatccctc
      841 acatcgcccc tcggctacaa cgcttctctc tcgaggcatc gtagctcggc ttgtccagaa
      901 gtgcttaccc cccgagatca tcatggaata tggtgaggaa gtattagaag aaataaaaaa
      961 ttcgaagcat aacacaccaa gaaaaaaaac aaaccccagc agaatcagga tagctttggg
     1021 aaatgaagca agcacagtgc aagaggaaga acaagataga aagggtagcc atactgattt
     1081 ggaaagcatt aatgaaaatt tagttgaaag tgcattgaga agagtaaaca gagaagaaaa
     1141 aggtaataag tcagtccatc tgaggaaagc cagttcacca aatcttcata gacgacagtg
     1201 ggagaaaaat gtacccaata cagctcttac agctttggaa aatgcatcca tactcacctc
     1261 cagtttaaca gcagaggacg atagaggtgg ttctgtaata aagtacagca aaaatactac
     1321 tcgtaagcag tggctcaaag agacccctga cactttgttg aacatcctta agaatgctga
     1381 tctcagcttg gcttttcaaa catacacaat atatagacca ggttcagaag ggttcttgaa
     1441 aggccccctg tctgaagaaa cagaagcatc ggacagtgtt gatggaggtc acgattctgt
     1501 cattttggat ccagagcgac ttgagcctgg gctagatgag gaggacacgg actttgagga
     1561 ggaagatgac aaccccgact gggtgtcaga gctgaagaag cgagctggat ggcaaggcct
     1621 gtgcgacaga taatgcctga ggaaatgttc ctgagtcacg ctgaggagag gcttcactca
     1681 ggagttcatg ctgagatgat catgagttca tgcgacgtat attttccttt ggaaacagaa
     1741 tgaagcagag gaaactctta atacttaaaa tcgttcttga ttagtatcgt gagtttgaaa
     1801 agtctagaac tcctgtaagt ttttgaactc aagggagaag gtatagtgga atgagtgtga
     1861 gcatcgggct ttgcagtccc atagaacaga aatgggatgc tagcgtgcca ctacctactt
     1921 gtgtgattgt gggaaattac ttaacctctt caagccccaa tttcctcaac cataaaatga
     1981 agataataat gcctacctca gagggatgct gaccacagac ctttatagca gcccgtatga
     2041 tattattcac attatgatat gtgtttatta ttatgtgact ctttttacat ttcctaaagg
     2101 tttgagaatt aaatatattt aattatga
//
