LOCUS       NM_001424269            2183 bp    mRNA    linear   PRI 17-JUL-2024
DEFINITION  Homo sapiens NIMA related kinase 3 (NEK3), transcript variant 15,
            mRNA.
ACCESSION   NM_001424269
VERSION     NM_001424269.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Melo-Hanchuk,T.D., Martins,M.B., Cunha,L.L., Soares,F.A.,
            Ward,L.S., Vassallo,J. and Kobarg,J.
  TITLE     Expression of the NEK family in normal and cancer tissue: an
            immunohistochemical study
  JOURNAL   BMC Cancer 20 (1), 23 (2020)
   PUBMED   31906878
  REMARK    GeneRIF: the NEK protein kinases emerge as important proteins in
            thyroid cancer development and may help to identify malignancy and
            aggressiveness features during diagnosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Rapaport,D., Fichtman,B., Weidberg,H., Sprecher,E. and Horowitz,M.
  TITLE     NEK3-mediated SNAP29 phosphorylation modulates its membrane
            association and SNARE fusion dependent processes
  JOURNAL   Biochem Biophys Res Commun 497 (2), 605-611 (2018)
   PUBMED   29454964
  REMARK    GeneRIF: NEK3 kinase phosphorylates SNAP29 on its serine 105.
            NEK3-mediated phosphorylation determines membrane localization of
            SNAP29. Membrane-associated SNAP29 regulates Golgi and focal
            adhesion structures.
REFERENCE   3  (bases 1 to 2183)
  AUTHORS   Cao,Y., Song,J., Chen,J., Xiao,J., Ni,J. and Wu,C.
  TITLE     Overexpression of NEK3 is associated with poor prognosis in
            patients with gastric cancer
  JOURNAL   Medicine (Baltimore) 97 (3), e9630 (2018)
   PUBMED   29504992
  REMARK    GeneRIF: The data demonstrate that NEK3 is overexpressed in gastric
            cancer, which promotes the malignancy of gastric cancer
REFERENCE   4  (bases 1 to 2183)
  AUTHORS   Harrington,K.M. and Clevenger,C.V.
  TITLE     Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory
            Phosphorylation Site That Modulates Focal Adhesion Remodeling
            Necessary for Breast Cancer Cell Migration
  JOURNAL   J Biol Chem 291 (41), 21388-21406 (2016)
   PUBMED   27489110
  REMARK    GeneRIF: these data support a modulatory role for phosphorylation
            at NEK3 Thr-165 in focal adhesion maturation and/or turnover to
            promote breast cancer cell migration.
REFERENCE   5  (bases 1 to 2183)
  AUTHORS   Benjamin,S., Weidberg,H., Rapaport,D., Pekar,O., Nudelman,M.,
            Segal,D., Hirschberg,K., Katzav,S., Ehrlich,M. and Horowitz,M.
  TITLE     EHD2 mediates trafficking from the plasma membrane by modulating
            Rac1 activity
  JOURNAL   Biochem J 439 (3), 433-442 (2011)
   PUBMED   21756249
  REMARK    GeneRIF: EHD2 interacts with Nek3 [NIMA (never in mitosis in
            Aspergillus nidulans)-related kinase 3], a serine/threonine kinase.
REFERENCE   6  (bases 1 to 2183)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   7  (bases 1 to 2183)
  AUTHORS   Kimura,M. and Okano,Y.
  TITLE     Molecular cloning and characterization of the human NIMA-related
            protein kinase 3 gene (NEK3)
  JOURNAL   Cytogenet Cell Genet 95 (3-4), 177-182 (2001)
   PUBMED   12063396
  REMARK    GeneRIF: Molecular cloning and characterization of the human
            NIMA-related protein kinase 3 gene (NEK3).
REFERENCE   8  (bases 1 to 2183)
  AUTHORS   Tanaka,K. and Nigg,E.A.
  TITLE     Cloning and characterization of the murine Nek3 protein kinase, a
            novel member of the NIMA family of putative cell cycle regulators
  JOURNAL   J Biol Chem 274 (19), 13491-13497 (1999)
   PUBMED   10224116
REFERENCE   9  (bases 1 to 2183)
  AUTHORS   Schultz,S.J., Fry,A.M., Sutterlin,C., Ried,T. and Nigg,E.A.
  TITLE     Cell cycle-dependent expression of Nek2, a novel human protein
            kinase related to the NIMA mitotic regulator of Aspergillus
            nidulans
  JOURNAL   Cell Growth Differ 5 (6), 625-635 (1994)
   PUBMED   7522034
REFERENCE   10 (bases 1 to 2183)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CP068265.2.
            
            Summary: This gene encodes a member of the NimA (never in mitosis
            A) family of serine/threonine protein kinases. The encoded protein
            differs from other NimA family members in that it is not cell cycle
            regulated and is found primarily in the cytoplasm. The kinase is
            activated by prolactin stimulation, leading to phosphorylation of
            VAV2 guanine nucleotide exchange factor, paxillin, and activation
            of the RAC1 GTPase. Two functional alleles for this gene have been
            identified in humans. The reference genome assembly (GRCh38)
            represents a functional allele that is associated with the
            inclusion of an additional coding exon in protein-coding
            transcripts, compared to an alternate functional allele that lacks
            the exon. [provided by RefSeq, Sep 2019].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB072828.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            polymorphic pseudogene :: based on alignments, homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-161               CP068265.2         51374126-51374286   c
            162-335             CP068265.2         51370906-51371079   c
            336-429             CP068265.2         51368911-51369004   c
            430-527             CP068265.2         51368726-51368823   c
            528-611             CP068265.2         51367440-51367523   c
            612-679             CP068265.2         51366156-51366223   c
            680-766             CP068265.2         51365977-51366063   c
            767-821             CP068265.2         51363246-51363300   c
            822-1022            CP068265.2         51359522-51359722   c
            1023-1094           CP068265.2         51358747-51358818   c
            1095-1197           CP068265.2         51351633-51351735   c
            1198-1341           CP068265.2         51350949-51351092   c
            1342-1476           CP068265.2         51350562-51350696   c
            1477-1603           CP068265.2         51348522-51348648   c
            1604-2183           CP068265.2         51347480-51348059   c
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.3"
     gene            1..2183
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /note="NIMA related kinase 3"
                     /db_xref="GeneID:4752"
                     /db_xref="HGNC:HGNC:7746"
                     /db_xref="MIM:604044"
     exon            1..161
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            162..335
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..1688
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /EC_number="2.7.11.1"
                     /note="isoform b is encoded by transcript variant 15;
                     serine/threonine-protein kinase NEK3; phosphorylase B
                     kinase kinase; glycogen synthase A kinase;
                     hydroxyalkyl-protein kinase; HSPK 36; nimA-related protein
                     kinase 3; never in mitosis A-related kinase 3; NIMA (never
                     in mitosis gene a)-related kinase 3"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek3 isoform b"
                     /protein_id="NP_001411198.1"
                     /db_xref="GeneID:4752"
                     /db_xref="HGNC:HGNC:7746"
                     /db_xref="MIM:604044"
                     /translation="MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNT
                     QNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPED
                     MILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFAC
                     TYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGC
                     ISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEE
                     VLEEIKNSKHNTPRKKQEEEQDRKGSHTDLESINENLVESALRRVNREEKGNKSVHLR
                     KASSPNLHRRQWEKNVPNTALTALENASILTSSLTAEDDRGGSVIKYSKNTTRKQWLK
                     ETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDP
                     ERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR"
     exon            336..429
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            430..527
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            528..611
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            612..679
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            680..766
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            767..821
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1022
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1094
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1197
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1341
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1476
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1603
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..2183
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2163..2168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /note="hexamer: ATTAAA"
     polyA_site      2183
                     /gene="NEK3"
                     /gene_synonym="HSPK36"
                     /note="major polyA site"
ORIGIN      
        1 agacgcgccc cggacgcgcg gccgcgcccc tcacttccgc cccgcggtct ggaccactcg
       61 ccgcaaaccc gcaagtccct cgccgccttg gggtctgggc gcgcggtgcc gtgggggtca
      121 gcagggcgga gcggcttttc caggagaaag ggccctcacg gtttgggaga gcccatggtg
      181 actgcgtgag tggagcccag ctgtgtggat gccccagcat ggatgactac atggtcctga
      241 gaatgattgg ggagggctcc ttcggcagag ctcttttggt tcagcatgaa agcagtaatc
      301 agatgtttgc catgaaagaa ataaggcttc ccaagtcttt ctctaataca cagaattcta
      361 ggaaggaggc tgttctttta gccaaaatga aacaccctaa tattgttgcc ttcaaagaat
      421 catttgaagc tgaaggacac ttgtatattg tgatggaata ctgtgatgga ggggatctaa
      481 tgcaaaagat taaacagcag aaaggaaagt tatttcctga agacatgata cttaattggt
      541 ttacccaaat gtgccttgga gtaaatcaca ttcacaagaa acgtgtgcta cacagagata
      601 tcaagtccaa gaatatcttc ctcactcaga atggaaaagt gaaattggga gactttggat
      661 ctgcccgtct tctctccaat ccgatggcat ttgcttgtac ctatgtggga actccttatt
      721 atgtgcctcc agaaatttgg gaaaacctgc cttataacaa taaaagtgac atctggtcct
      781 tgggttgcat cctgtatgaa ctctgtaccc ttaagcatcc atttcaggca aatagttgga
      841 aaaatcttat cctcaaagta tgtcaagggt gcatcagtcc actgccgtct cattactcct
      901 atgaacttca gttcctagtc aagcagatgt ttaaaaggaa tccctcacat cgcccctcgg
      961 ctacaacgct tctctctcga ggcatcgtag ctcggcttgt ccagaagtgc ttaccccccg
     1021 agatcatcat ggaatatggt gaggaagtat tagaagaaat aaaaaattcg aagcataaca
     1081 caccaagaaa aaaacaagag gaagaacaag atagaaaggg tagccatact gatttggaaa
     1141 gcattaatga aaatttagtt gaaagtgcat tgagaagagt aaacagagaa gaaaaaggta
     1201 ataagtcagt ccatctgagg aaagccagtt caccaaatct tcatagacga cagtgggaga
     1261 aaaatgtacc caatacagct cttacagctt tggaaaatgc atccatactc acctccagtt
     1321 taacagcaga ggacgataga ggtggttctg taataaagta cagcaaaaat actactcgta
     1381 agcagtggct caaagagacc cctgacactt tgttgaacat ccttaagaat gctgatctca
     1441 gcttggcttt tcaaacatac acaatatata gaccaggttc agaagggttc ttgaaaggcc
     1501 ccctgtctga agaaacagaa gcatcggaca gtgttgatgg aggtcacgat tctgtcattt
     1561 tggatccaga gcgacttgag cctgggctag atgaggagga cacggacttt gaggaggaag
     1621 atgacaaccc cgactgggtg tcagagctga agaagcgagc tggatggcaa ggcctgtgcg
     1681 acagataatg cctgaggaaa tgttcctgag tcacgctgag gagaggcttc actcaggagt
     1741 tcatgctgag atgatcatga gttcatgcga cgtatatttt cctttggaaa cagaatgaag
     1801 cagaggaaac tcttaatact taaaatcgtt cttgattagt atcgtgagtt tgaaaagtct
     1861 agaactcctg taagtttttg aactcaaggg agaaggtata gtggaatgag tgtgagcatc
     1921 gggctttgca gtcccataga acagaaatgg gatgctagcg tgccactacc tacttgtgtg
     1981 attgtgggaa attacttaac ctcttcaagc cccaatttcc tcaaccataa aatgaagata
     2041 ataatgccta cctcagaggg atgctgacca cagaccttta tagcagcccg tatgatatta
     2101 ttcacattat gatatgtgtt tattattatg tgactctttt tacatttcct aaaggtttga
     2161 gaattaaata tatttaatta tga
//
