LOCUS       NM_002497               2134 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1,
            mRNA.
ACCESSION   NM_002497
VERSION     NM_002497.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Zhang,Y., Yu,H., He,M., Liu,W., Xiao,S., Wang,X., Huang,P. and
            Huang,Q.
  TITLE     NEK2 promotes TP53 ubiquitination to enhance the proliferation and
            migration of TP53 wild-type glioblastoma cells
  JOURNAL   Neoplasma 71 (3), 255-265 (2024)
   PUBMED   38764296
  REMARK    GeneRIF: NEK2 promotes TP53 ubiquitination to enhance the
            proliferation and migration of TP53 wild-type glioblastoma cells.
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Wang,M., Sun,F., Zhang,S., Zhang,X., Sun,Y., Yu,T., Li,Y.,
            Jiang,A., Qiao,P., Ren,C. and Yang,T.
  TITLE     NEK2 promotes the development of ovarian endometriosis and impairs
            decidualization by phosphorylating FOXO1
  JOURNAL   Cell Mol Life Sci 81 (1), 237 (2024)
   PUBMED   38795132
  REMARK    GeneRIF: NEK2 promotes the development of ovarian endometriosis and
            impairs decidualization by phosphorylating FOXO1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2134)
  AUTHORS   Tang,P., Zheng,G., Xu,C., Yu,N., Du,J., Hu,L., Zhou,Z. and Zheng,Y.
  TITLE     Function of NEK2 in clear cell renal cell carcinoma and its effect
            on the tumor microenvironment
  JOURNAL   Medicine (Baltimore) 103 (20), e37939 (2024)
   PUBMED   38758909
  REMARK    GeneRIF: Function of NEK2 in clear cell renal cell carcinoma and
            its effect on the tumor microenvironment.
REFERENCE   4  (bases 1 to 2134)
  AUTHORS   Fu,H., Zhang,M., Liu,X., Yang,Y. and Xing,Y.
  TITLE     Abnormal methylation mediated upregulation of LINC00857 boosts
            malignant progression of lung adenocarcinoma by modulating the
            miR-486-5p/NEK2 axis
  JOURNAL   Clin Respir J 18 (5), e13765 (2024)
   PUBMED   38721812
  REMARK    GeneRIF: Abnormal methylation mediated upregulation of LINC00857
            boosts malignant progression of lung adenocarcinoma by modulating
            the miR-486-5p/NEK2 axis.
REFERENCE   5  (bases 1 to 2134)
  AUTHORS   Noguchi,K., Fukazawa,H., Murakami,Y. and Uehara,Y.
  TITLE     Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells
  JOURNAL   J Biol Chem 279 (31), 32716-32727 (2004)
   PUBMED   15161910
  REMARK    GeneRIF: Nucleolar Nek11 is a novel target of Nek2A in
            G1/S-arrested cells
REFERENCE   6  (bases 1 to 2134)
  AUTHORS   Hames,R.S. and Fry,A.M.
  TITLE     Alternative splice variants of the human centrosome kinase Nek2
            exhibit distinct patterns of expression in mitosis
  JOURNAL   Biochem J 361 (Pt 1), 77-85 (2002)
   PUBMED   11742531
REFERENCE   7  (bases 1 to 2134)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
REFERENCE   8  (bases 1 to 2134)
  AUTHORS   Schultz,S.J., Fry,A.M., Sutterlin,C., Ried,T. and Nigg,E.A.
  TITLE     Cell cycle-dependent expression of Nek2, a novel human protein
            kinase related to the NIMA mitotic regulator of Aspergillus
            nidulans
  JOURNAL   Cell Growth Differ 5 (6), 625-635 (1994)
   PUBMED   7522034
REFERENCE   9  (bases 1 to 2134)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
REFERENCE   10 (bases 1 to 2134)
  AUTHORS   Fahim,A.T., Daiger,S.P. and Weleber,R.G.
  TITLE     Nonsyndromic Retinitis Pigmentosa Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301590
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC407297.1, BT019729.1,
            AK223353.1 and BC043502.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_002497.3.
            
            Summary: This gene encodes a serine/threonine-protein kinase that
            is involved in mitotic regulation. This protein is localized to the
            centrosome, and undetectable during G1 phase, but accumulates
            progressively throughout the S phase, reaching maximal levels in
            late G2 phase. Alternatively spliced transcript variants encoding
            different isoforms with distinct C-termini have been noted for this
            gene. [provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longest isoform (1, also known as
            NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be
            responsible for its nucleolar localization (PMID:15161910).
            Isoforms 1 and 2 (which differ at the C-terminus) have also been
            reported to exhibit distinct pattern of expression during mitosis
            (PMID:11742531).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043502.1, AK223353.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366999.9/ ENSP00000355966.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-568               DC407297.1         10-577
            569-1480            BT019729.1         427-1338
            1481-2063           AK223353.1         1451-2033
            2064-2134           BC043502.1         2062-2132
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.3"
     gene            1..2134
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /note="NIMA related kinase 2"
                     /db_xref="GeneID:4751"
                     /db_xref="HGNC:HGNC:7745"
                     /db_xref="MIM:604043"
     exon            1..238
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..1480
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase Nek2; nimA-like protein
                     kinase 1; nimA-related protein kinase 2; NIMA (never in
                     mitosis gene a)-related kinase 2; protein phosphatase 1,
                     regulatory subunit 111"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek2 isoform 1"
                     /protein_id="NP_002488.1"
                     /db_xref="CCDS:CCDS1500.1"
                     /db_xref="GeneID:4751"
                     /db_xref="HGNC:HGNC:7745"
                     /db_xref="MIM:604043"
                     /translation="MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSM
                     TEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGT
                     KERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDF
                     GLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTA
                     FSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVA
                     DEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQEL
                     CVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKE
                     NIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR"
     exon            239..456
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     exon            457..697
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     exon            698..780
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     exon            781..907
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1127
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1253
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..2134
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2107..2112
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /note="hexamer: AATAAA"
     polyA_site      2134
                     /gene="NEK2"
                     /gene_synonym="HsPK21; NEK2A; NLK1; PPP1R111; RP67"
                     /note="major polyA site"
ORIGIN      
        1 aaacggggcc caaggcaggg gtggcgggtc agtgctgctc gggggcttct ccatccaggt
       61 ccctggagtt cctggtccct ggagctccgc acttggcggc gcaacctgcg tgaggcagcg
      121 cgactctggc gactggccgg ccatgccttc ccgggctgag gactatgaag tgttgtacac
      181 cattggcaca ggctcctacg gccgctgcca gaagatccgg aggaagagtg atggcaagat
      241 attagtttgg aaagaacttg actatggctc catgacagaa gctgagaaac agatgcttgt
      301 ttctgaagtg aatttgcttc gtgaactgaa acatccaaac atcgttcgtt actatgatcg
      361 gattattgac cggaccaata caacactgta cattgtaatg gaatattgtg aaggagggga
      421 tctggctagt gtaattacaa agggaaccaa ggaaaggcaa tacttagatg aagagtttgt
      481 tcttcgagtg atgactcagt tgactctggc cctgaaggaa tgccacagac gaagtgatgg
      541 tggtcatacc gtattgcatc gggatctgaa accagccaat gttttcctgg atggcaagca
      601 aaacgtcaag cttggagact ttgggctagc tagaatatta aaccatgaca cgagttttgc
      661 aaaaacattt gttggcacac cttattacat gtctcctgaa caaatgaatc gcatgtccta
      721 caatgagaaa tcagatatct ggtcattggg ctgcttgctg tatgagttat gtgcattaat
      781 gcctccattt acagctttta gccagaaaga actcgctggg aaaatcagag aaggcaaatt
      841 caggcgaatt ccataccgtt actctgatga attgaatgaa attattacga ggatgttaaa
      901 cttaaaggat taccatcgac cttctgttga agaaattctt gagaaccctt taatagcaga
      961 tttggttgca gacgagcaaa gaagaaatct tgagagaaga gggcgacaat taggagagcc
     1021 agaaaaatcg caggattcca gccctgtatt gagtgagctg aaactgaagg aaattcagtt
     1081 acaggagcga gagcgagctc tcaaagcaag agaagaaaga ttggagcaga aagaacagga
     1141 gctttgtgtt cgtgagagac tagcagagga caaactggct agagcagaaa atctgttgaa
     1201 gaactacagc ttgctaaagg aacggaagtt cctgtctctg gcaagtaatc cagaacttct
     1261 taatcttcca tcctcagtaa ttaagaagaa agttcatttc agtggggaaa gtaaagagaa
     1321 catcatgagg agtgagaatt ctgagagtca gctcacatct aagtccaagt gcaaggacct
     1381 gaagaaaagg cttcacgctg cccagctgcg ggctcaagcc ctgtcagata ttgagaaaaa
     1441 ttaccaactg aaaagcagac agatcctggg catgcgctag ccaggtagag agacacagag
     1501 ctgtgtacag gatgtaatat taccaacctt taaagactga tattcaaatg ctgtagtgtt
     1561 gaatacttgg ttccatgagc catgcctttc tgtatagtac acatgatatt tcggaattgg
     1621 ttttactgtt cttcagcaac tattgtacaa aatgttcaca tttaattttt ctttcttctt
     1681 ttaagaacat attataaaaa gaatactttc ttggttgggc ttttaatcct gtgtgtgatt
     1741 actagtagga acatgagatg tgacattcta aatcttggga gaaaaaataa tgttaggaaa
     1801 aaaatattta tgcaggaaga gtagcactca ctgaatagtt ttaaatgact gagtggtatg
     1861 cttacaattg tcatgtctag atttaaattt taagtctgag attttaaatg tttttgagct
     1921 tagaaaaccc agttagatgc aatttggtca ttaataccat gacatcttgc ttataaatat
     1981 tccattgctc tgtagttcaa atctgttagc tttgtgaaaa ttcatcactg tgatgtttgt
     2041 attctttttt tttttctgtt taacagaata tgagctgtct gtcatttacc tacttctttc
     2101 ccactaaata aaagaattct tcagtttccc tgta
//
