LOCUS       NM_001394966            8332 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 8,
            mRNA.
ACCESSION   NM_001394966 XM_017005771
VERSION     NM_001394966.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Dutt,P., Haider,N., Mouaaz,S., Podmore,L. and Stambolic,V.
  TITLE     beta-catenin turnover is regulated by Nek10-mediated tyrosine
            phosphorylation in A549 lung adenocarcinoma cells
  JOURNAL   Proc Natl Acad Sci U S A 121 (19), e2300606121 (2024)
   PUBMED   38683979
  REMARK    GeneRIF: beta-catenin turnover is regulated by Nek10-mediated
            tyrosine phosphorylation in A549 lung adenocarcinoma cells.
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Haider,N., Dutt,P., van de Kooij,B., Ho,J., Palomero,L.,
            Pujana,M.A., Yaffe,M. and Stambolic,V.
  TITLE     NEK10 tyrosine phosphorylates p53 and controls its transcriptional
            activity
  JOURNAL   Oncogene 39 (30), 5252-5266 (2020)
   PUBMED   32561851
  REMARK    GeneRIF: NEK10 tyrosine phosphorylates p53 and controls its
            transcriptional activity.
REFERENCE   3  (bases 1 to 8332)
  AUTHORS   Al Mutairi,F., Alkhalaf,R., Alkhorayyef,A., Alroqi,F., Yusra,A.,
            Umair,M., Nouf,F., Khan,A., Meshael,A., Hamad,A., Monira,A.,
            Asiri,A., Alhamoudi,K.M. and Alfadhel,M.
  TITLE     Homozygous truncating NEK10 mutation, associated with primary
            ciliary dyskinesia: a case report
  JOURNAL   BMC Pulm Med 20 (1), 141 (2020)
   PUBMED   32414360
  REMARK    GeneRIF: Homozygous truncating NEK10 mutation, associated with
            primary ciliary dyskinesia: a case report.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8332)
  AUTHORS   Chivukula,R.R., Montoro,D.T., Leung,H.M., Yang,J., Shamseldin,H.E.,
            Taylor,M.S., Dougherty,G.W., Zariwala,M.A., Carson,J.,
            Daniels,M.L.A., Sears,P.R., Black,K.E., Hariri,L.P., Almogarri,I.,
            Frenkel,E.M., Vinarsky,V., Omran,H., Knowles,M.R., Tearney,G.J.,
            Alkuraya,F.S. and Sabatini,D.M.
  TITLE     A human ciliopathy reveals essential functions for NEK10 in airway
            mucociliary clearance
  JOURNAL   Nat Med 26 (2), 244-251 (2020)
   PUBMED   31959991
  REMARK    GeneRIF: analysis of a bronchiectasis syndrome caused by mutations
            that inactivate NIMA-related kinase 10 (NEK10), a protein kinase
            with previously unknown in vivo functions in mammals
            Erratum:[Nat Med. 2020 Feb;26(2):300. doi:
            10.1038/s41591-020-0773-z.. PMID: 31996837]
REFERENCE   5  (bases 1 to 8332)
  AUTHORS   Moniz,L.S. and Stambolic,V.
  TITLE     Nek10 mediates G2/M cell cycle arrest and MEK autoactivation in
            response to UV irradiation
  JOURNAL   Mol Cell Biol 31 (1), 30-42 (2011)
   PUBMED   20956560
  REMARK    GeneRIF: Nek10 physically associated with Raf-1 and MEK1 in a
            Raf-1-dependent manner, and the formation of this complex was
            necessary for Nek10-mediated MEK1 activation.
REFERENCE   6  (bases 1 to 8332)
  AUTHORS   Ahmed,S., Thomas,G., Ghoussaini,M., Healey,C.S., Humphreys,M.K.,
            Platte,R., Morrison,J., Maranian,M., Pooley,K.A., Luben,R.,
            Eccles,D., Evans,D.G., Fletcher,O., Johnson,N., dos Santos
            Silva,I., Peto,J., Stratton,M.R., Rahman,N., Jacobs,K.,
            Prentice,R., Anderson,G.L., Rajkovic,A., Curb,J.D., Ziegler,R.G.,
            Berg,C.D., Buys,S.S., McCarty,C.A., Feigelson,H.S., Calle,E.E.,
            Thun,M.J., Diver,W.R., Bojesen,S., Nordestgaard,B.G., Flyger,H.,
            Dork,T., Schurmann,P., Hillemanns,P., Karstens,J.H.,
            Bogdanova,N.V., Antonenkova,N.N., Zalutsky,I.V., Bermisheva,M.,
            Fedorova,S., Khusnutdinova,E., Kang,D., Yoo,K.Y., Noh,D.Y.,
            Ahn,S.H., Devilee,P., van Asperen,C.J., Tollenaar,R.A.,
            Seynaeve,C., Garcia-Closas,M., Lissowska,J., Brinton,L.,
            Peplonska,B., Nevanlinna,H., Heikkinen,T., Aittomaki,K.,
            Blomqvist,C., Hopper,J.L., Southey,M.C., Smith,L., Spurdle,A.B.,
            Schmidt,M.K., Broeks,A., van Hien,R.R., Cornelissen,S., Milne,R.L.,
            Ribas,G., Gonzalez-Neira,A., Benitez,J., Schmutzler,R.K.,
            Burwinkel,B., Bartram,C.R., Meindl,A., Brauch,H., Justenhoven,C.,
            Hamann,U., Chang-Claude,J., Hein,R., Wang-Gohrke,S., Lindblom,A.,
            Margolin,S., Mannermaa,A., Kosma,V.M., Kataja,V., Olson,J.E.,
            Wang,X., Fredericksen,Z., Giles,G.G., Severi,G., Baglietto,L.,
            English,D.R., Hankinson,S.E., Cox,D.G., Kraft,P., Vatten,L.J.,
            Hveem,K., Kumle,M., Sigurdson,A., Doody,M., Bhatti,P.,
            Alexander,B.H., Hooning,M.J., van den Ouweland,A.M.,
            Oldenburg,R.A., Schutte,M., Hall,P., Czene,K., Liu,J., Li,Y.,
            Cox,A., Elliott,G., Brock,I., Reed,M.W., Shen,C.Y., Yu,J.C.,
            Hsu,G.C., Chen,S.T., Anton-Culver,H., Ziogas,A., Andrulis,I.L.,
            Knight,J.A., Beesley,J., Goode,E.L., Couch,F., Chenevix-Trench,G.,
            Hoover,R.N., Ponder,B.A., Hunter,D.J., Pharoah,P.D., Dunning,A.M.,
            Chanock,S.J. and Easton,D.F.
  CONSRTM   SEARCH; GENICA Consortium; kConFab; Australian Ovarian Cancer Study
            Group
  TITLE     Newly discovered breast cancer susceptibility loci on 3p24 and
            17q23.2
  JOURNAL   Nat Genet 41 (5), 585-590 (2009)
   PUBMED   19330027
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099535.2, AC093555.2 and AC098931.3.
            
            On May 12, 2021 this sequence version replaced XM_017005771.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.249664.1,
                                           SRR1660807.148930.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000691995.1/ ENSP00000509472.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               AC099535.2         36157-36315         c
            160-267             AC099535.2         19744-19851         c
            268-328             AC099535.2         19397-19457         c
            329-459             AC099535.2         13018-13148         c
            460-558             AC099535.2         11204-11302         c
            559-643             AC093555.2         159839-159923       c
            644-685             AC093555.2         151959-152000       c
            686-764             AC093555.2         149761-149839       c
            765-832             AC093555.2         148611-148678       c
            833-912             AC093555.2         146588-146667       c
            913-999             AC093555.2         145521-145607       c
            1000-1224           AC093555.2         142409-142633       c
            1225-1364           AC093555.2         139358-139497       c
            1365-1426           AC093555.2         134841-134902       c
            1427-1504           AC093555.2         133275-133352       c
            1505-1569           AC093555.2         131250-131314       c
            1570-1672           AC093555.2         129146-129248       c
            1673-1801           AC093555.2         128924-129052       c
            1802-1939           AC093555.2         128279-128416       c
            1940-1985           AC093555.2         125360-125405       c
            1986-2107           AC093555.2         122502-122623       c
            2108-2210           AC093555.2         122264-122366       c
            2211-2286           AC093555.2         93958-94033         c
            2287-2416           AC093555.2         40090-40219         c
            2417-2487           AC093555.2         39172-39242         c
            2488-2701           AC093555.2         29691-29904         c
            2702-2885           AC093555.2         12312-12495         c
            2886-2972           AC093555.2         12101-12187         c
            2973-3027           AC093555.2         9481-9535           c
            3028-3065           AC098931.3         176146-176183       c
            3066-3166           AC098931.3         154927-155027       c
            3167-3277           AC098931.3         145325-145435       c
            3278-3386           AC098931.3         133205-133313       c
            3387-3439           AC098931.3         129520-129572       c
            3440-3495           AC098931.3         129385-129440       c
            3496-8332           AC098931.3         119929-124765       c
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p24.1"
     gene            1..8332
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /note="NIMA related kinase 10"
                     /db_xref="GeneID:152110"
                     /db_xref="HGNC:HGNC:18592"
                     /db_xref="MIM:618726"
     exon            1..159
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            160..267
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..3544
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /EC_number="2.7.11.1"
                     /note="isoform 8 is encoded by transcript variant 8;
                     serine/threonine-protein kinase Nek10; nimA-related
                     protein kinase 10; never in mitosis A-related kinase 10;
                     NIMA (never in mitosis gene a)- related kinase 10"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek10 isoform 8"
                     /protein_id="NP_001381895.1"
                     /db_xref="CCDS:CCDS93229.1"
                     /db_xref="GeneID:152110"
                     /db_xref="HGNC:HGNC:18592"
                     /db_xref="MIM:618726"
                     /translation="MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQ
                     LPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQ
                     RKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIEN
                     LAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLL
                     GARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRL
                     LCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGI
                     KQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACC
                     AALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSME
                     RNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQ
                     NKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDS
                     SVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHH
                     HFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQE
                     NSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATK
                     IVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQ
                     LSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSE
                     LSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSS
                     SSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPASAGIAVSQRKVRQIS
                     DPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQ
                     GSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVL
                     EESGYYNFTSNRYHSYPWGTKNHPTKR"
     exon            268..328
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            329..459
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            460..558
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            559..643
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            644..685
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            686..764
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            765..832
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            833..912
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            913..999
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1224
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1364
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1426
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1504
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1569
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1672
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1801
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1939
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..1985
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2107
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2210
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2286
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2416
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2487
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2701
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2885
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..2972
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3027
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3065
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3066..3166
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3277
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3278..3386
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3387..3439
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3440..3495
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..8332
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4710..4715
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /note="hexamer: ATTATA"
     polyA_site      4731
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /note="major polyA site"
     regulatory      7459..7464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /note="hexamer: AGTAAA"
     polyA_site      7480
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /note="major polyA site"
     regulatory      8302..8307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK10"
                     /gene_synonym="CILD44"
                     /note="hexamer: ATTAAA"
     polyA_site      8332
                     /gene="NEK10"
                     /gene_synonym="CILD44"
ORIGIN      
        1 ggccgcggtg tccctgaggt tgaggggacc gggaataggc tggggggagg acgggacggg
       61 ctgagactgg acgggacccc cggtctgcag cagcaggtga cagcagcagg gacaatgata
      121 aggagattgg cctgaaggag ggaccgtccc tcccgcgcgg aatgcgatgt taattctggg
      181 gcattgatgt tttacaatgc ctgatcaaga taaaaaggtg aagaccacag aaaaatcaac
      241 tgataaacag caagaaatca ccatcaggga ctattcagat cttaaaagac ttcggtgcct
      301 tttgaacgtc caatcaagca aacaacagct tccagccatt aacttcgata gtgcccaaaa
      361 tagcatgacg aagtctgagc ccgccatcag ggcgggtgga cacagagctc ggggtcagtg
      421 gcatgaatcc acagaagctg ttgaacttga aaattttagt ataaactaca agaatgagag
      481 aaatttcagc aaacatcctc agcgtaaact atttcaggag atctttaccg ccttggtgaa
      541 aaatagactc ataagcagag agtgggttaa tcgagcccca tctattcatt ttctgagagt
      601 gttaatctgt ctgaggctac taatgaggga tccatgttat caggaaatac tccatagctt
      661 gggtgggatt gaaaacctag ctcagtatat ggagattgta gccaatgagt acctcggcta
      721 tggagaagag cagcacactg tggacaagct ggtcaacatg acatatattt ttcaaaaact
      781 tgctgcagtc aaagatcaaa gagaatgggt caccacaagt ggagcccaca agacattagt
      841 aaatttactt ggtgcccgag atactaatgt tctattgggt tcccttctgg ctctggctag
      901 tttagcagaa agtcaagaat gtagggagaa gataagtgaa ctcaacattg tagaaaatct
      961 gttgatgatt ttacatgaat atgacttgct ttctaaaaga ctaacagcgg agttgctgcg
     1021 cctactttgt gcagagcccc aggtgaaaga gcaggtgaag ctctatgagg ggataccggt
     1081 cctcctcagt ctgctccact ctgaccactt gaagctcctc tggagcattg tctggattct
     1141 ggtacaggtt tgtgaggacc ctgagaccag cgtggaaatt cgcatttggg gaggcatcaa
     1201 acagcttctt catattttac aaggagacag aaattttgtt tctgatcact cctccattgg
     1261 aagcctgtcc agtgcaaatg ctgcaggccg aatccagcag cttcatttat cagaagactt
     1321 gagccctagg gaaatacaag aaaatacttt ctcacttcaa gcagcctgct gtgctgccct
     1381 cactgagctg gtgctcaatg acaccaatgc ccaccaggtg gttcaggaaa atggtgtata
     1441 tacaatagca aaattaattt taccaaataa gcaaaagaat gcagcaaaaa gtaatctatt
     1501 acagtgttat gctttcagag ccttgagatt tctcttcagt atggaaagaa acagaccact
     1561 ctttaaaaga cttttcccca cagacttgtt tgagatcttc attgacatag ggcattatgt
     1621 acgtgatatc agtgcttatg aagaattggt atccaagctg aatttattag tggaggatga
     1681 actgaagcaa attgctgaaa atattgaaag cattaatcag aacaaagctc ctttgaaata
     1741 tataggcaac tatgcaattt tggatcatct tggaagtgga gcttttggct gtgtttacaa
     1801 ggttagaaag catagtggtc aaaatctttt agcaatgaaa gaggtcaatt tacataaccc
     1861 agcatttgga aaggataaga aagatcgaga cagcagcgta aggaatattg tttctgaatt
     1921 aacaataatt aaagagcagc tttatcatcc caacattgta cgttattaca aaacatttct
     1981 ggaaaatgat aggttgtaca tagttatgga gctgatagaa ggagccccgc ttggagagca
     2041 tttcagttct ttgaaggaaa aacatcacca ttttactgaa gaaagactat ggaaaatatt
     2101 tatacagctg tgcttagctc ttcgatactt acacaaggag aagaggattg tccatagaga
     2161 tctgacacca aacaacatta tgttggggga taaggacaaa gtaaccgtta ctgactttgg
     2221 cctggcaaag caaaaacaag aaaacagtaa actcacctct gtggttggaa caatcctgta
     2281 ttcttgcccc gaggtactga agagtgagcc gtatggggag aaggctgatg tctgggcagt
     2341 aggctgcatc ctttatcaga tggcgacttt gagtcccccc ttctacagca ctaacatgct
     2401 gtccttggct acaaaaatag tggaggcggt atatgaacca gtcccagaag gtatctactc
     2461 tgaaaaagta acagacacca tcagcaggtg cctcactcct gatgcggaag ctcgtccaga
     2521 tattgtagaa gtcagttcga tgatatcaga tgtcatgatg aaatatttag acaacttatc
     2581 tacatcccag ttgtccttgg aaaagaagct agaacgggaa cgaagacgca cacaaaggta
     2641 ttttatggaa gccaaccgga acaccgtcac atgtcaccat gagctggctg ttctatctca
     2701 cgagaccttt gagaaggcaa gtttgagtag cagcagcagt ggagcagcca gcctgaaaag
     2761 tgaactttca gaaagcgcag acctgccccc tgaaggcttc caggcctcct atggtaaaga
     2821 cgaagacagg gcctgtgacg aaatcctgtc agatgataac ttcaacctgg aaaatgctga
     2881 gaaagataca tattcagagg tagatgatga attggacatt tcggataact ccagcagctc
     2941 cagttcaagc cctctgaaag aatctacatt caacatttta aagagaagtt ttagtgcttc
     3001 aggaggagaa agacaatccc aaacaaggga cttcactgga ggaacaggat caagaccaag
     3061 accagcatca gcaggaattg ctgtgtccca gaggaaagtg cgtcagatca gtgatcctat
     3121 tcagcagata ttaattcagc tgcacaaaat aatctatatc acacagcttc ctccagcttt
     3181 gcaccacaat ttgaaaagaa gggttataga gagattcaag aaatccctct tcagccagca
     3241 gagtaaccct tgtaatttga aatctgaaat taaaaagtta tctcagggat ctccagaacc
     3301 gattgagccc aactttttca cagcagatta ccatttatta catcgttcat ccggtggaaa
     3361 cagcctgtcc ccaaatgacc ctacaggttt accaaccagc attgaattgg aggaaggaat
     3421 aacatatgaa cagatgcaga ctgtgattga agaagtcctt gaggaaagtg gctattacaa
     3481 ttttacatct aacaggtatc attcctatcc atgggggacc aagaatcacc caaccaaaag
     3541 atgaaaatgc tgcattttga gtggacttga ttttctcagt gaagttcaag ttctggactt
     3601 cagccgctat tgcaagatgc ccaaggattg ggtgctgcta gagggtgtgg aaaagaccaa
     3661 gatgccatgg ggcctgcagg acttctttct gggggtcctg tgctggagta tatgacagct
     3721 gcggtacttg agggcttcat tgccagaaca cattatatac aggatgtcag agctaccagt
     3781 gtgctgctgg gagaaaatgc tgcaaaattc atcttttgga gggtgggggg aaaacccaaa
     3841 aacaacaaca aaaaaactct cttacagaat tttccttaac attaaaaaaa acttgtcata
     3901 tttttcaaag gcacatttga tactcagaat tgctaaaagt atatttaaag acatctagcc
     3961 ttccatatgt aaaaaatatt ttagataaag acagttacag gactcagaat aatatattgg
     4021 gtgattcact gtatcctcca tttttacatt taaagaaatt caaaatacgt gattaaattt
     4081 tttttttttt aatggatggg ttggttctcg cttaaccact tgggtgctag tggtcttgaa
     4141 ctgtgataca gtgtagacag tatttgtaac cttgcacagc ctctgggggt aactgtcaaa
     4201 ctgtcagatt taacttagac taatagaata tattgctgta cagtgtgtgt atatgtttat
     4261 atatgagggc ggaaggagag aggttttgat tagactaatg ctttgctgct aatcaaactt
     4321 atagtatttt tagagcactt tgaagagttt atagaatcta tgaaaaagat gcaatattcc
     4381 tcatcctaaa tccctcctta ttacaattac ctaaattgtt tatgaattgt ttaggatttt
     4441 gaaatgaaaa taagttaaat ccctttttta ctcattgagg cagctcagtg gaagtgaaat
     4501 cagaatgcca aactgtgtat catcctttat gccgggagaa ttatgatgaa aatctgctag
     4561 attctcactg ggcttaaact ccttctcttc aatcagcttc ttaaaactct ggtgtaatgt
     4621 caagaaatac ccttattgcg aaatctgtct acacttctag ctacctaaag agcaatttat
     4681 tctacataat tagtaatctg aaagttgaaa ttatactttt tatttacttt tcatggagtt
     4741 ctgtcatctt tatttacact gaattttttt agtatgattg aatcgatttt agttcaatgt
     4801 atttacagtg gttgaaagac cataaccttc tttctgtata gtatgaaaat ctacactatt
     4861 tttaaatgag catatttaat acttaaatta tatccaaatt ccctttaatc atgcactaat
     4921 gcttttgaag attgtcaatg gatctttctt taagccaaat taacaattac agatacactg
     4981 taatgtaaga taatagatgc aaatacagta atgaaaattt cagtttcagc tttcaaacta
     5041 attctatcca tatgtgaaat tcataaaatc aaaatgctat gatattgatt tacccaggca
     5101 aaattttatt tctgttccaa gttaatttga acctgcactt ataattcttt ttatctttca
     5161 gtgtatgaaa ggggcacatt tcttcttcat gcaggaagca cagacctgga ggggcacaaa
     5221 ttctaattgt ggggtgagag tttgaaatca agttatttcc caaatggtag tatatagtaa
     5281 acagaattta ggaaagaata gtattcaaaa ggtgtaccgt ggtaagggaa gataaatggt
     5341 ttacttgaag atgttttaaa gtgtatatag ttggccttat tgaccagaaa gtgagcaaaa
     5401 tgtttccatc taactctttt tttttttttt tttttttaag acagagtctc actctgttgc
     5461 ccaggcttga gtgcaatggc gagatcttaa cttactgcaa cctccgccac ccaggttcaa
     5521 gcaattctcc tgcctcagcc tcccgagtaa ctgggattac aggcatgtgc tgccatgccc
     5581 agctaatttt tgtgttttta gtagagatgg ggttacacca tgttggccag gctggtctca
     5641 aactcctgac ctcaggtgat ctgcctgcct cggcctccca aagtgctggg attagaggcg
     5701 tgagccactg tgcccagcca ttgcatgtaa ctcttttagg tgataggaat gggcatttat
     5761 tatgctagaa gtcagtgaaa gttaagtaag cagaagctgt cctttttaac aacagcttca
     5821 ggcctatcaa ctctatgtgg atagatgtat tgttttgcta aaagtatgaa tatgctctat
     5881 ttgaattctc cctaaactac cacaatcact gagtctatat tttcccatgt ctctaagaga
     5941 aagtcaggct ctggcaaaag caccattttg tacatagagt cacatcccaa cagagaagtt
     6001 aacttttcag aagcctttct aacacaaata tggagcacct cccctcatga ccaagaatga
     6061 tcattttgtt ttgattctcc aaactgtcta ttagtgcaaa ggagttgtta catgaacttc
     6121 atagacctct taatcttttc ttgattgcat ttatatattt tttccctttc tcttgtataa
     6181 tttttaatga tttgttgtgc aatgaaattc ctatgaatgc acctaatttc cgtaactgaa
     6241 tatttgcatg gaatagaata tgaatcagtc ttttcagatt acttttgaaa cacaaatgct
     6301 cccgttaatt atgtttcgac ccaccagctc tttctgaatt atgtgtactt gaaaccttaa
     6361 tttgcttttt aaaagcaaaa tgtttcattc tcagggaagt gactgcagca ggaatatttg
     6421 cttgcttgtt aactttatct tcctccaggc atgtagtctt caaatggagt cttcaaggga
     6481 atagtgacgt ggacacttgg acccactgct tgtggatgaa tttcagaaac ctagagaaat
     6541 gctgcttgtt tattgtggac acttgtatgg cattaggcag tgtttacaga atgtttaggc
     6601 attctaaagc agaacacaat taagaaacac tgttaggaaa tttactcaaa tgatataatt
     6661 gattaagagt taggtcttcc tataagtatc atctatgact cattaaatac tatgaatttt
     6721 gatgtccaaa aacaaataca ggtctgatta tgtacaattc cagaaatatc attaattaat
     6781 caccactcat ttttaagatg tgtgaagact gtaatattgg ctagtgaatt ttatcagtat
     6841 taatatgcat agaacccaca ttcctctttt tgatttgatg tattatagca tgtatgtatt
     6901 gctatttttc tctttttttg aagtggtgag gaatcatgca cagtcaatat gctgggttcc
     6961 tttagaaatg actttagctc ctgtctgaag gcaggaaaaa cttcttttta aggaactttc
     7021 atcattgcct tttacttttt ctatgatggt tttcatgagc actgaaatca cttggagagg
     7081 caatgcaaag aaatctatct gaaacagctt cttggcaccc tggagttaca gctatgaagg
     7141 gctccaacgt aagggaagct taatgcttcc gaatattgac attgactcct tgggtgaaat
     7201 tttgtccaaa tataaaattc ttcatgttca acaactaaat gtaataaatg aatttcatat
     7261 atacttacat gatatctttg agattaaatt aattatcctt ttgtaggaac tgacagcttt
     7321 gggtagatta ttttttcagt tgaaatgtgt tgctaacaat atgcttacac ttgaacgctg
     7381 tttttcatat tgataggaag acacaaattt ctcagggaaa cagctttgtg ataaaggaat
     7441 tcttatgtgt gtcattacag taaattgcat attgtaaata tgactgttgt ggttgattat
     7501 agtcctgctg tgatgttgtt ttgagatttg caagagggac aggaaagatt ttatgagttt
     7561 catggggtgt ctgatggata ttgccaattt acatattttc tgagttcatg ggcaatagtt
     7621 gtttttaaaa atgaagatca cgtaccctaa aatcacagct gcatacttgc tatgtgaggt
     7681 atactagatg ctttattgca tgtatgtaac aattgtctaa taatataaaa aatttaagat
     7741 acagttcatt gctttatcct aaaagtaaat gaactgtagt cttaattcaa aactgaatgt
     7801 tattcctcaa aacatgagaa actgcctttt tctctccttt ccttttgtac tgtgaagatg
     7861 tgcactggcc ccagctactg tatttagcga taacagaagt acgtgactgt ttaaatgctg
     7921 tcatttggaa atagcttacc tttctttctc ctaaacaaag atagaatttt gtctttatgt
     7981 atggaggatt atggctgact gtcattttga caatcaacaa atccagatgg atacttggaa
     8041 ttgatatggt gagttcacaa atgaaaaatt agccgaagtt tttgcaaaca tgtgaaaaac
     8101 tggtcccata aaacatgcag agagaagttt gtgccagaaa taacaaacca agaggaacaa
     8161 ctgctttaag gccttggcac ctttaaggca gactgtgaag gtcacttaca aaatgatatt
     8221 cttaacccat gatttagctc tgcatttcaa gtgccatttg agtcaagatt ctgaaataga
     8281 atataaagct tgtaaatggc tattaaattt tagctaaaac tgttgccctc ca
//
