LOCUS       NM_001199397            6096 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1,
            mRNA.
ACCESSION   NM_001199397
VERSION     NM_001199397.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Zelina,P., de Ruiter,A.A., Kolsteeg,C., van Ginneken,I., Vos,H.R.,
            Supiot,L.F., Burgering,B.M.T., Meye,F.J., Veldink,J.H., van den
            Berg,L.H. and Pasterkamp,R.J.
  TITLE     ALS-associated C21ORF2 variant disrupts DNA damage repair,
            mitochondrial metabolism, neuronal excitability and NEK1 levels in
            human motor neurons
  JOURNAL   Acta Neuropathol Commun 12 (1), 144 (2024)
   PUBMED   39227882
  REMARK    GeneRIF: ALS-associated C21ORF2 variant disrupts DNA damage repair,
            mitochondrial metabolism, neuronal excitability and NEK1 levels in
            human motor neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   He,Q., Zhou,Y., Jin,J., Tian,Q., Li,H., Hou,B. and Xie,A.
  TITLE     Association between NEK1 gene polymorphisms and the potential risk
            of sporadic Parkinson's disease in the Chinese Northern Han
            population: A case-control study
  JOURNAL   Neurosci Lett 837, 137913 (2024)
   PUBMED   39032803
  REMARK    GeneRIF: Association between NEK1 gene polymorphisms and the
            potential risk of sporadic Parkinson's disease in the Chinese
            Northern Han population: A case-control study.
REFERENCE   3  (bases 1 to 6096)
  AUTHORS   Gregorczyk,M., Pastore,G., Munoz,I., Carroll,T., Streubel,J.,
            Munro,M., Lis,P., Lange,S., Lamoliatte,F., Macartney,T., Toth,R.,
            Brown,F., Hastie,J., Pereira,G., Durocher,D. and Rouse,J.
  TITLE     Functional characterization of C21ORF2 association with the NEK1
            kinase mutated in human in diseases
  JOURNAL   Life Sci Alliance 6 (7), e202201740 (2023)
   PUBMED   37188479
  REMARK    GeneRIF: Functional characterization of C21ORF2 association with
            the NEK1 kinase mutated in human in diseases.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6096)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   5  (bases 1 to 6096)
  AUTHORS   Jiang,Q., Lin,J., Wei,Q., Li,C., Hou,Y., Zhang,L., Ou,R., Liu,K.,
            Yang,T., Xiao,Y., Hadano,S. and Shang,H.
  TITLE     Genetic and clinical characteristics of ALS patients with NEK1 gene
            variants
  JOURNAL   Neurobiol Aging 123, 191-199 (2023)
   PUBMED   36443167
  REMARK    GeneRIF: Genetic and clinical characteristics of ALS patients with
            NEK1 gene variants.
REFERENCE   6  (bases 1 to 6096)
  AUTHORS   Surpili,M.J., Delben,T.M. and Kobarg,J.
  TITLE     Identification of proteins that interact with the central
            coiled-coil region of the human protein kinase NEK1
  JOURNAL   Biochemistry 42 (51), 15369-15376 (2003)
   PUBMED   14690447
  REMARK    GeneRIF: Mapping studies of NEK1 regulatory domain indicate
            interaction with most of the proteins known to take part in
            etiology of polycystic kidney disease, in double-strand DNA break
            repair at the G2/M cell-cycle transition phase, or in neural cell
            development.
REFERENCE   7  (bases 1 to 6096)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
REFERENCE   8  (bases 1 to 6096)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
REFERENCE   9  (bases 1 to 6096)
  AUTHORS   Siddique,N. and Siddique,T.
  TITLE     Amyotrophic Lateral Sclerosis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301623
REFERENCE   10 (bases 1 to 6096)
  AUTHORS   Letwin,K., Mizzen,L., Motro,B., Ben-David,Y., Bernstein,A. and
            Pawson,T.
  TITLE     A mammalian dual specificity protein kinase, Nek1, is related to
            the NIMA cell cycle regulator and highly expressed in meiotic germ
            cells
  JOURNAL   EMBO J 11 (10), 3521-3531 (1992)
   PUBMED   1382974
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC084724.6, AC116621.8 and
            AC116615.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 25, 2019 this sequence version replaced NM_001199397.2.
            
            Summary: The protein encoded by this gene is a serine/threonine
            kinase involved in cell cycle regulation. The encoded protein is
            found in a centrosomal complex with FEZ1, a neuronal protein that
            plays a role in axonal development. Defects in this gene are a
            cause of polycystic kidney disease (PKD). Several transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Dec 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1 and 6 both encode the same isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR933642.1, SRR1803617.111804.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000507142.6/ ENSP00000424757.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-362               AC084724.6         125063-125424       c
            363-486             AC084724.6         124861-124984       c
            487-651             AC084724.6         115355-115519       c
            652-748             AC084724.6         114849-114945       c
            749-846             AC084724.6         111941-112038       c
            847-930             AC084724.6         103567-103650       c
            931-998             AC084724.6         102288-102355       c
            999-1085            AC084724.6         101490-101576       c
            1086-1140           AC084724.6         100400-100454       c
            1141-1341           AC084724.6         98190-98390         c
            1342-1402           AC084724.6         93683-93743         c
            1403-1554           AC084724.6         89769-89920         c
            1555-1614           AC084724.6         74978-75037         c
            1615-1674           AC084724.6         74673-74732         c
            1675-1725           AC084724.6         74528-74578         c
            1726-1800           AC084724.6         74321-74395         c
            1801-1964           AC084724.6         68773-68936         c
            1965-2096           AC084724.6         68561-68692         c
            2097-2199           AC084724.6         50650-50752         c
            2200-2283           AC084724.6         21610-21693         c
            2284-2367           AC084724.6         21089-21172         c
            2368-2445           AC084724.6         20556-20633         c
            2446-2541           AC084724.6         19878-19973         c
            2542-2673           AC116621.8         43379-43510         c
            2674-2739           AC116621.8         41408-41473         c
            2740-2968           AC116621.8         41100-41328         c
            2969-3121           AC116621.8         27219-27371         c
            3122-3298           AC116621.8         2059-2235           c
            3299-3419           AC116615.5         127844-127964       c
            3420-3508           AC116615.5         120445-120533       c
            3509-3756           AC116615.5         118852-119099       c
            3757-3908           AC116615.5         100895-101046       c
            3909-4117           AC116615.5         95951-96159         c
            4118-4248           AC116615.5         94820-94950         c
            4249-4381           AC116615.5         94524-94656         c
            4382-6096           AC116615.5         87108-88822         c
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q33"
     gene            1..6096
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /note="NIMA related kinase 1"
                     /db_xref="GeneID:4750"
                     /db_xref="HGNC:HGNC:7744"
                     /db_xref="MIM:604588"
     exon            1..362
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            363..486
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            487..651
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     CDS             535..4395
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase Nek1;
                     protein-serine/threonine kinase; nimA-related protein
                     kinase 1; renal carcinoma antigen NY-REN-55; never in
                     mitosis A-related kinase 1; NIMA (never in mitosis gene
                     a)-related kinase 1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek1 isoform 1"
                     /protein_id="NP_001186326.1"
                     /db_xref="CCDS:CCDS56351.1"
                     /db_xref="GeneID:4750"
                     /db_xref="HGNC:HGNC:7744"
                     /db_xref="MIM:604588"
                     /translation="MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKE
                     REESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQE
                     DQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELA
                     RTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISG
                     SFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFC
                     LKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKP
                     LQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKR
                     QEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIF
                     DQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLK
                     RLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYG
                     GRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSE
                     EADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSP
                     PLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREI
                     LRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPL
                     DELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLE
                     NTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSL
                     KLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQEN
                     EVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQV
                     QSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLF
                     RTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLR
                     EQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASE
                     CECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNE
                     HQHLYAKILHLVMADGAYQEDNDE"
     exon            652..748
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            749..846
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            847..930
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            931..998
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1085
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1140
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1341
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1402
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1554
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1614
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1674
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1725
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1800
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1964
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2096
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2199
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2283
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2367
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2445
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2541
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2673
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2739
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2968
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..3121
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3122..3298
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3299..3419
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3508
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3509..3756
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3757..3908
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            3909..4117
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4118..4248
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4249..4381
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     exon            4382..6096
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5491..5496
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /note="hexamer: AATAAA"
     polyA_site      5515
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
     regulatory      5608..5613
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /note="hexamer: ATTAAA"
     polyA_site      5632
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /note="major polyA site"
     regulatory      6073..6078
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
                     /note="hexamer: AATAAA"
     polyA_site      6096
                     /gene="NEK1"
                     /gene_synonym="ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;
                     SRTD6"
ORIGIN      
        1 gcattcgctc cagggttttg ggaccctagg ttgcggagtc cttaccctac cctggcctct
       61 cgagcagttg tccccataac tcggaatcta gagccgctgt tgcgaggcag gagcacgtgg
      121 cagtcaagta gcttcccagt cccgaacgcc gcccgtcccc accccgccgt ggccactagc
      181 aacgacctct gtgaagttgg agaggcggta acggaggcac tccccctgct gcaccccgcc
      241 gtttctacgg ggctcagaaa ccagtttgtt tgtttcgtcg gggtagtgtc gacctgtctt
      301 acgggcgtcg cccgagacag gacggagtca aacccgtggt atcaactgaa gacgagtgtc
      361 aggatgtcat tttcaaaatg cgggatggta cctctgcttt attaagcccc gtaggaagac
      421 tgccacacct agactgatgc ttattagtca tcaccgttat tcctactaac gtcctgtgtc
      481 actgagtttt ttaaatgtct agcatatctg taaagatgcc ttagaaaaag aatcatggag
      541 aagtatgtta gactacagaa gattggagaa ggttcatttg gaaaagccat tcttgttaaa
      601 tctacagaag atggcagaca gtatgttatc aaggaaatta acatctcaag aatgtccagt
      661 aaagaaagag aagaatcaag gagagaagtt gcagtattgg caaacatgaa gcatccaaat
      721 attgtccagt atagagaatc atttgaagaa aatggctctc tctacatagt aatggattac
      781 tgtgagggag gggatctgtt taagcgaata aatgctcaga aaggcgtttt gtttcaagag
      841 gatcagattt tggactggtt tgtacagata tgtttggccc tgaaacatgt acatgataga
      901 aaaattcttc atcgagacat taaatctcag aacatatttt taactaaaga tggaacagta
      961 caacttggag attttggaat tgctagagtt cttaatagta ctgtagagct ggctcgaact
     1021 tgcataggga ccccatacta cttgtcacct gaaatctgtg aaaacaaacc ttacaataat
     1081 aaaagtgaca tttgggctct ggggtgtgtc ctttatgagc tgtgtacact taaacatgct
     1141 tttgaagctg gcagtatgaa aaacctggta ctgaagataa tatctggatc ttttccacct
     1201 gtgtctttgc attattccta tgatctccgc agtttggtgt ctcagttatt taaaagaaat
     1261 cctagggata gaccatcagt caactccata ttggagaaag gttttatagc caaacgcatt
     1321 gaaaagtttc tctctcctca gcttattgca gaagaatttt gtctaaaaac attttcgaag
     1381 tttggatcac agcctatacc agctaaaaga ccagcttcag gacaaaactc gatttctgtt
     1441 atgcctgctc agaaaattac aaagcctgcc gctaaatatg gaataccttt agcatataag
     1501 aaatatggag ataaaaaatt acacgaaaag aaaccactgc aaaaacataa acaggcccat
     1561 caaactccag agaagagagt gaatactgga gaagaaagga ggaaaatatc tgaggaagca
     1621 gcaagaaaga gaaggctgga atttattgaa aaagaaaaga aacaaaagga tcagattatt
     1681 agtttaatga aggctgaaca aatgaaaagg caagaaaagg aaaggttgga aagaataaat
     1741 agggccaggg aacaaggatg gagaaatgtg ctaagtgctg gtggaagtgg tgaagtaaag
     1801 gctccttttc tgggcagtgg agggactata gctccatcat ctttttcttc tcgaggacag
     1861 tatgaacatt accatgccat ttttgaccaa atgcagcaac aaagagcaga agataatgaa
     1921 gctaaatgga aaagagaaat atatggtcga ggtcttccag aaagaggaat tctgcctgga
     1981 gttcgtccag gatttcctta tggggctgca ggtcatcacc attttcctga tgctgatgat
     2041 attagaaaaa ctttgaaaag attgaaggcg gtgtctaaac aagccaatgc aaacaggcaa
     2101 aaagggcagc tagctgtaga aagagctaaa caagtagaag agttcctgca gcgaaaacgg
     2161 gaagctatgc agaataaagc tcgagccgaa ggacatatgg gaatcctgca aaacctggca
     2221 gctatgtatg gaggcaggcc cagctcttca agaggaggga agccaagaaa caaagaggaa
     2281 gaggtttatc tggcaagact gaggcaaata agactacaga atttcaatga gcgccaacag
     2341 attaaagcca aacttcgtgg tgaaaagaaa gaagctaatc attctgaagg acaagaagga
     2401 agtgaagagg ctgacatgag gcgcaaaaaa atcgaatcac tgaaggccca tgcaaatgca
     2461 cgtgctgctg tactaaaaga acaactagaa cgaaagagaa aggaggctta tgagagagaa
     2521 aaaaaagtgt gggaagagca tttggtggct aaaggagtta agagttctga tgtttctcca
     2581 cctttgggac agcatgaaac aggtggctct ccatcaaagc aacagatgag atctgttatt
     2641 tctgtaactt cagctttgaa agaagttggc gtggacagta gtttaactga tacccgggaa
     2701 acttcagaag agatgcaaaa gaccaacaat gctatttcaa gtaagcgaga aatacttcgt
     2761 agattaaatg aaaatcttaa agctcaagaa gatgaaaaag gaaagcagaa tctctctgat
     2821 acttttgaga taaatgttca tgaagatgcc aaagagcatg aaaaagaaaa atcagtttca
     2881 tctgatcgca agaagtggga ggcaggaggt caacttgtga ttcctctgga tgagttaaca
     2941 ctagatacat ccttctctac aactgaaaga catacagtgg gagaagttat taaattaggt
     3001 cctaatggat ctccaagaag agcctggggg aaaagtccga cagattctgt tctaaagata
     3061 cttggagaag ctgaactaca acttcagaca gaactattag aaaatacaac tattagaagt
     3121 gagatttctc ccgaagggga aaagtacaaa cccttaatta ctggagaaaa aaaagtacaa
     3181 tgtatttcac atgaaataaa cccatcagct attgttgatt ctcctgttga gacaaaaagt
     3241 cccgagttca gtgaggcatc tccacagatg tcattgaaac tggaaggaaa tttagaagaa
     3301 cctgatgatt tggaaacaga aattctacaa gagccaagtg gaacaaacaa agatgagagc
     3361 ttgccatgca ctattactga tgtgtggatt agtgaggaaa aagaaacaaa ggaaactcag
     3421 tcggcagata ggatcaccat tcaggaaaat gaagtttctg aagatggagt ctcgagtact
     3481 gtggaccaac ttagtgacat tcatatagag cctggaacca atgattctca gcactctaaa
     3541 tgtgatgtag ataagtctgt gcaaccggaa ccatttttcc ataaggtggt tcattctgaa
     3601 cacttgaact tagtccctca agttcaatca gttcagtgtt caccagaaga atcctttgca
     3661 tttcgatctc actcgcattt accaccaaaa aataaaaaca agaattcctt gctgattgga
     3721 ctttcaactg gtctgtttga tgcaaacaac ccaaagatgt taaggacatg ttcacttcca
     3781 gatctctcaa agctgttcag aacccttatg gatgttccca ccgtaggaga tgttcgtcaa
     3841 gacaatcttg aaatagatga aattgaagat gaaaacatta aagaaggacc ttctgattct
     3901 gaagacattg tgtttgaaga aactgacaca gatttacaag agctgcaggc ctcgatggaa
     3961 cagttactta gggaacaacc tggtgaagaa tacagtgaag aagaagagtc agtcttgaag
     4021 aacagtgatg tggagccaac tgcaaatggg acagatgtgg cagatgaaga tgacaatccc
     4081 agcagtgaaa gtgccctgaa cgaagaatgg cactcagata acagtgatgg tgaaattgct
     4141 agtgaatgtg aatgcgatag tgtctttaac catttagagg aactgagact tcatctggag
     4201 caggaaatgg gctttgaaaa attctttgag gtttatgaga aaataaaggc tattcatgaa
     4261 gatgaagatg aaaatattga aatttgttca aaaatagttc aaaatatttt gggaaatgaa
     4321 catcagcatc tttatgccaa gattcttcat ttagtcatgg cagatggagc ctaccaagaa
     4381 gataatgatg aataatcctc aaaatgtttt ttaatcctca actatatgaa agcatttgaa
     4441 tttggcttat cagaataaca agcttcagtg ggaaatacag caattattta tttaaaaaat
     4501 cagatttaag atggactttc ttattgcatg aaaaagatgg agaaacatgc catttttcag
     4561 tgaagattct aatattttat ctattttgtt cattgaattc catggttaaa tctcataaaa
     4621 tatatacttt attaaatcat ccaaccaaag cataggaaac attgacccag aacctgactt
     4681 aatggttttg aagatttact atgcaatagg gtaactttga gtttcagcaa atgtctttag
     4741 gttgaaggaa ttacctatgt catgaaggac ctgtctgtgg tttttcaatg gagtctttaa
     4801 gcatgatctt ttttctgtct agtacttgtt ttcattctgg ccagcagttc tacattaaat
     4861 caccttgtca agggctctgt ttacatctat acattttgaa gatgaaattt ttagccttaa
     4921 agtttatatt ctcaagtcct tttacaatca gtgtgtctcc tgaactagca cacaggctgt
     4981 agaaacagtc ttagaaatca ttgaaagatt tgattatgaa agaatagcaa aattatattt
     5041 cttgacatat aaaaagttgg tttaatgcct ttatttctct ttaaggacca gaaccaggaa
     5101 tactatatcg aaaaattagt ctgtggattt aacactgact tagcatatag cttaaagttg
     5161 ctcttttggt ttttaacttc ctccatacat aagcttcaag gacaataaga tgttaaaaag
     5221 gaggaaataa ttatttttat tttgacactg tgacagtttt ggtaactagg atcctaggga
     5281 gggaaatgtt tgcctgttga acttctttct gttatgagag gatttagtta ggtcattaag
     5341 atgttgatca cacagcttca atcacaatat gccaagtata acctggtttc gttagaggtg
     5401 tctacagtcc agatgttctt cgtaataaaa gcaaagtttt tgaacctctg agtccaaagc
     5461 aggctggttg gcataatatg taatttgaaa aataaaatct tatcttgcag cactatcagt
     5521 atgttgaatt tattatgtat attatttcta atatccgaaa ctaaatactt gattttttaa
     5581 tatgtgtgtt tattttatga tattgctatt aaatttttat tatctacctg aagtatatca
     5641 attgtctttt atttattatt ttacttttct caatagaagg cttttacatc aagctgataa
     5701 tacttttgtt ggttttgtac ctgatgattt ctttcttcat aatggaagaa aatgaattca
     5761 atattttaat tctttacaat cctgaaagat gggcatgatt ttggggaggt tgtgtattaa
     5821 ttcttaaact attacaacta ttagaaaagg gtataatatg gtagaaaaaa attgttttag
     5881 ccatatttca aaagatgtta tatatgtgca ggaataagga tgatcaataa gatttgtgcc
     5941 atctccagtc gattagcagt gtgtgactct ggagtgttgt tatggaaaga ttatgccagt
     6001 gaggaaaagg gctatttttc agtaaggtct attataagtg ttggaattga catgtggaag
     6061 catctgtaga ttaataaatg ttagcattaa atgggc
//
