LOCUS       NM_145043               2699 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens nei like DNA glycosylase 2 (NEIL2), transcript variant
            1, mRNA.
ACCESSION   NM_145043
VERSION     NM_145043.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Hua,A.B. and Sweasy,J.B.
  TITLE     Functional roles and cancer variants of the bifunctional
            glycosylase NEIL2
  JOURNAL   Environ Mol Mutagen 65 Suppl 1, 40-56 (2024)
   PUBMED   37310399
  REMARK    GeneRIF: Functional roles and cancer variants of the bifunctional
            glycosylase NEIL2.
            Review article
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Eckenroth,B.E., Bumgarner,J.D., Matsumoto-Elliott,O., David,S.S.
            and Doublie,S.
  TITLE     Structural and biochemical insights into NEIL2's preference for
            abasic sites
  JOURNAL   Nucleic Acids Res 51 (22), 12508-12521 (2023)
   PUBMED   37971311
  REMARK    GeneRIF: Structural and biochemical insights into NEIL2's
            preference for abasic sites.
REFERENCE   3  (bases 1 to 2699)
  AUTHORS   Tapryal,N., Chakraborty,A., Saha,K., Islam,A., Pan,L., Hosoki,K.,
            Sayed,I.M., Duran,J.M., Alcantara,J., Castillo,V., Tindle,C.,
            Sarker,A.H., Wakamiya,M., Cardenas,V.J., Sharma,G., Crotty
            Alexander,L.E., Sur,S., Sahoo,D., Ghosh,G., Das,S., Ghosh,P.,
            Boldogh,I. and Hazra,T.K.
  TITLE     The DNA glycosylase NEIL2 is protective during SARS-CoV-2 infection
  JOURNAL   Nat Commun 14 (1), 8169 (2023)
   PUBMED   38071370
  REMARK    GeneRIF: The DNA glycosylase NEIL2 is protective during SARS-CoV-2
            infection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2699)
  AUTHORS   Kakhkharova,Z.I., Zharkov,D.O. and Grin,I.R.
  TITLE     A Low-Activity Polymorphic Variant of Human NEIL2 DNA Glycosylase
  JOURNAL   Int J Mol Sci 23 (4), 2212 (2022)
   PUBMED   35216329
  REMARK    GeneRIF: A Low-Activity Polymorphic Variant of Human NEIL2 DNA
            Glycosylase.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2699)
  AUTHORS   Dutta,A., Yang,C., Sengupta,S., Mitra,S. and Hegde,M.L.
  TITLE     New paradigms in the repair of oxidative damage in human genome:
            mechanisms ensuring repair of mutagenic base lesions during
            replication and involvement of accessory proteins
  JOURNAL   Cell Mol Life Sci 72 (9), 1679-1698 (2015)
   PUBMED   25575562
  REMARK    Review article
REFERENCE   6  (bases 1 to 2699)
  AUTHORS   Katafuchi,A., Nakano,T., Masaoka,A., Terato,H., Iwai,S., Hanaoka,F.
            and Ide,H.
  TITLE     Differential specificity of human and Escherichia coli endonuclease
            III and VIII homologues for oxidative base lesions
  JOURNAL   J Biol Chem 279 (14), 14464-14471 (2004)
   PUBMED   14734554
  REMARK    GeneRIF: hNEIL2 recognized an apurinic/apyrimidinic site
            exclusively, and the activity for 5-hydroxyuracil and
            guanine-derived formamidopyrimidine was marginal
REFERENCE   7  (bases 1 to 2699)
  AUTHORS   Dou,H., Mitra,S. and Hazra,T.K.
  TITLE     Repair of oxidized bases in DNA bubble structures by human DNA
            glycosylases NEIL1 and NEIL2
  JOURNAL   J Biol Chem 278 (50), 49679-49684 (2003)
   PUBMED   14522990
  REMARK    GeneRIF: NEIL1 and NEIL2 are preferentially involved in repair of
            lesions in DNA bubbles generated during transcription and/or
            replication
REFERENCE   8  (bases 1 to 2699)
  AUTHORS   Takao,M., Kanno,S., Kobayashi,K., Zhang,Q.M., Yonei,S., van der
            Horst,G.T. and Yasui,A.
  TITLE     A back-up glycosylase in Nth1 knock-out mice is a functional Nei
            (endonuclease VIII) homologue
  JOURNAL   J Biol Chem 277 (44), 42205-42213 (2002)
   PUBMED   12200441
REFERENCE   9  (bases 1 to 2699)
  AUTHORS   Hazra,T.K., Kow,Y.W., Hatahet,Z., Imhoff,B., Boldogh,I.,
            Mokkapati,S.K., Mitra,S. and Izumi,T.
  TITLE     Identification and characterization of a novel human DNA
            glycosylase for repair of cytosine-derived lesions
  JOURNAL   J Biol Chem 277 (34), 30417-30420 (2002)
   PUBMED   12097317
  REMARK    GeneRIF: NEIL2 is involved in global genome repair mainly for
            removing oxidative products of cytosine.
REFERENCE   10 (bases 1 to 2699)
  AUTHORS   Bandaru,V., Sunkara,S., Wallace,S.S. and Bond,J.P.
  TITLE     A novel human DNA glycosylase that removes oxidative DNA damage and
            is homologous to Escherichia coli endonuclease VIII
  JOURNAL   DNA Repair (Amst) 1 (7), 517-529 (2002)
   PUBMED   12509226
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB452586.1, AK056206.1,
            DA205315.1, AB079070.1 and AC069185.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_145043.3.
            
            Summary: This gene encodes a member of the Fpg/Nei family of DNA
            glycosylases. These glycosylases initiate the first step in base
            excision repair by cleaving oxidatively damaged bases and
            introducing a DNA strand break via their abasic site lyase
            activity. This enzyme is primarily associated with DNA repair
            during transcription and acts prefentially on cytosine-derived
            lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It
            contains an N-terminal catalytic domain, a hinge region, and a
            C-terminal DNA-binding domain with helix-two-turn-helix and zinc
            finger motifs. This enzyme interacts with the X-ray cross
            complementing factor 1 scaffold protein as part of a multi-protein
            DNA repair complex. A pseudogene of this gene has been identified.
            [provided by RefSeq, Mar 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Variants 1, 2, and
            8 encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK056206.1, SRR1660803.114464.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000284503.7/ ENSP00000284503.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DB452586.1         49-86
            39-402              AK056206.1         1-364
            403-685             DA205315.1         302-584
            686-1191            AK056206.1         648-1153
            1192-1627           AB079070.1         564-999
            1628-2699           AC069185.7         110191-111262
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p23.1"
     gene            1..2699
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /note="nei like DNA glycosylase 2"
                     /db_xref="GeneID:252969"
                     /db_xref="HGNC:HGNC:18956"
                     /db_xref="MIM:608933"
     exon            1..626
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /inference="alignment:Splign:2.1.0"
     exon            627..766
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /inference="alignment:Splign:2.1.0"
     CDS             629..1627
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /EC_number="4.2.99.18"
                     /note="isoform a is encoded by transcript variant 1;
                     endonuclease 8-like 2; nei homolog 2; nei endonuclease
                     VIII-like 2; nei-like protein 2; DNA glycosylase/AP lyase
                     Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2"
                     /codon_start=1
                     /product="endonuclease 8-like 2 isoform a"
                     /protein_id="NP_659480.1"
                     /db_xref="CCDS:CCDS5984.1"
                     /db_xref="GeneID:252969"
                     /db_xref="HGNC:HGNC:18956"
                     /db_xref="MIM:608933"
                     /translation="MPEGPLVRKFHHLVSPFVGQQVVKTGGSSKKLQPASLQSLWLQD
                     TQVHGKKLFLRFDLDEEMGPPGSSPTPEPPQKEVQKEGAADPKQVGEPSGQKTLDGSS
                     RSAELVPQGEDDSEYLERDAPAGDAGRWLRVSFGLFGSVWVNDFSRAKKANKRGDWRD
                     PSPRLVLHFGGGGFLAFYNCQLSWSSSPVVTPTCDILSEKFHRGQALEALGQAQPVCY
                     TLLDQRYFSGLGNIIKNEALYRAGIHPLSLGSVLSASRREVLVDHVVEFSTAWLQGKF
                     QGRPQHTQVYQKEQCPAGHQVMKEAFGPEDGLQRLTWWCPQCQPQLSEEPEQCQFS"
     exon            767..1119
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1316
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..2699
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2678..2683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /note="hexamer: AATAAA"
     polyA_site      2699
                     /gene="NEIL2"
                     /gene_synonym="NEH2; NEI2"
                     /note="major polyA site"
ORIGIN      
        1 aaatctccgc ccccagctgg agcggctgtg cgggctgcgt agcggtgctg ggtcgggccg
       61 acgtgccacc cacccggagc cggtgagtgc agccgcccgc cctccggtag atctgcggcc
      121 tggcggagaa gtcgggaggg gacaggaagg gagggcgggc cccgggccct cctccgtctc
      181 agccgcctgc ggaggtgctg cccacgcctg gaggccccca ctgaccctca gacccgcgtc
      241 tgcgcccctc tccccgcacc ccgaggcaga gttgggaaag cagtggtctt agacccccca
      301 cctcgggcac tcggaagaga acggcggaga caacccctcc tcttccctgg ctggcgcagc
      361 gccagcctcg agctcctcgg tagcccccgg gcagggaggg ccggagggtg ggcgcggcat
      421 cttcagcgac tcttcgaagt cccttccgcg tctcatcttt caaggctgtt gcagaggcgg
      481 cttgcttccc acctgtccat ctccataaaa atccctaaac gaaacatgcc cacgtgtccg
      541 gagattttca ggacttggtg catttcagat gaaggctttt ccagaagctt ccccgtagaa
      601 gaggatcagg catccaactg gttaagggat gccagaaggg ccgttggtga ggaaatttca
      661 ccatttggtc tccccctttg tgggtcagca ggtggtcaag acagggggca gcagtaagaa
      721 gctacagccc gccagcctgc agtctctgtg gctccaggac acccaggtcc atggaaagaa
      781 attattcctt agatttgatc tagatgaaga aatggggccc cctggcagca gcccaacacc
      841 agagcctcca caaaaagaag tgcagaagga aggggctgcg gacccaaagc aggtcgggga
      901 gcccagcggg cagaagaccc ttgatggatc ctcacggtct gcagagctcg tcccccaggg
      961 cgaggatgat tctgagtatt tggagagaga cgcccctgca ggagatgctg ggaggtggct
     1021 gcgtgtcagc tttggtttgt ttggcagcgt ttgggtgaac gatttctcca gagccaagaa
     1081 agccaacaag aggggggact ggagggaccc ttccccgagg ttggtcctgc actttggtgg
     1141 tggtggcttc ctggcatttt ataattgtca gttgtcttgg agctcttccc cagtggtcac
     1201 acccacctgt gacatcctgt ctgagaagtt ccatcgagga caagccttag aagctctagg
     1261 ccaggctcag cctgtctgct atacactgct ggaccagaga tacttctcag ggctagggaa
     1321 catcattaag aatgaagcct tgtacagagc tgggatccat cccctttctc tcggttcagt
     1381 cctgagtgcc tcgcgtcggg aggtcctggt ggatcacgtg gtggagttca gtacagcctg
     1441 gctgcagggc aagttccaag gcagaccgca gcacacacag gtctaccaga aagaacagtg
     1501 ccctgctggc caccaggtca tgaaggaggc gtttgggccc gaagatgggt tacagaggct
     1561 cacctggtgg tgcccgcagt gccagcccca gttgtcagag gagccagagc agtgccagtt
     1621 ctcctaagga gctggtggtg ctcctcacgg aaccttgccg cttggggaac ctgacgtcta
     1681 agtgtccaga aaggaggatg tgggcaggga cggggtacag aggatagtgt gggtcagagg
     1741 tgccagtagt ataatattcg tctccctgga gttatgttga aggcagagtt ttcatagggt
     1801 tagatttttt ttatcctttt ctagttcagt taattcatcc tgttgaattg caccatcgtg
     1861 aaagatggga aaaatcgtga tgatgggtaa ggggaaaact tcccggaagg caatggggca
     1921 aggaaaaaga aagcctatgg gaaatggctg tgctcccaac atagctttgc agatgatgtg
     1981 ggtttttttt ttttttttgg ttgtttgttt tgagagagag tcttgctctg tctccctggc
     2041 tagggtgtgg tggtgtgatc ttggctcacg gcagccttgc cctccctggc tcaagcagtc
     2101 ttcatctcag cctccagagt agctgggact acaggcatgt gatatgatgc tcggctgatt
     2161 tttgtttact ttttagagag atggggtctt gccatatttg ccaggctggt ttgaactcct
     2221 acaactcaag cattcctccc accttggtct cccaaaatgt tgggaccacg ggtgtgagcc
     2281 accgcgccca gctagctcct gtgttttgtt tttgttttgt aactttggtt gatgttaagg
     2341 ccctccattt tggaaagcag gaaaacagga tttttttttt tttatcttgt tccctggagg
     2401 atccagggat gaggatagag tggcctgaga gcagtgcttg gattcagcct cctgctgggt
     2461 ccttctgctg gatacaggca ccaagaggcg gtcgtggagc agggagctgc gccttcctgg
     2521 ggtgcccggt ggtgtgtaga gaaaagctgc ttgtttactc cttaagtcaa tgtattggtg
     2581 actgttgatt tgttgaacaa ttcaggaatc aagggctgtg gagaaactcc ctcatgttgt
     2641 tggcaacagg tgaatgaacc tagagcggtg acatgaaaat aaagctcact gttactcgc
//
