LOCUS       NM_006403               4522 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens neural precursor cell expressed, developmentally
            down-regulated 9 (NEDD9), transcript variant 1, mRNA.
ACCESSION   NM_006403
VERSION     NM_006403.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Liu,W. and Luo,G.
  TITLE     NEDD9 is transcriptionally regulated by HDAC4 and promotes breast
            cancer metastasis and macrophage M2 polarization via the
            FAK/NF-kappaB signaling pathway
  JOURNAL   Neoplasia 57, 101059 (2024)
   PUBMED   39326322
  REMARK    GeneRIF: NEDD9 is transcriptionally regulated by HDAC4 and promotes
            breast cancer metastasis and macrophage M2 polarization via the
            FAK/NF-kappaB signaling pathway.
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Tang,L., Xu,S., Wei,R., Fan,G., Zhou,J., Wei,X. and Xu,X.
  TITLE     Transcription factor 7 like 2 promotes metastasis in hepatocellular
            carcinoma via NEDD9-mediated activation of AKT/mTOR signaling
            pathway
  JOURNAL   Mol Med 30 (1), 108 (2024)
   PUBMED   39060928
  REMARK    GeneRIF: Transcription factor 7 like 2 promotes metastasis in
            hepatocellular carcinoma via NEDD9-mediated activation of AKT/mTOR
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4522)
  AUTHORS   Im,S., Cho,Y.K., Kang,D., Shin,G.Y., Jung,E.S., Song,K.Y., Lee,S.H.
            and Park,J.M.
  TITLE     Combined high NEDD9 expression and E-cadherin loss correlate with
            poor clinical outcome in gastric cancer
  JOURNAL   J Gastroenterol Hepatol 37 (12), 2255-2263 (2022)
   PUBMED   36203318
  REMARK    GeneRIF: Combined high NEDD9 expression and E-cadherin loss
            correlate with poor clinical outcome in gastric cancer.
REFERENCE   4  (bases 1 to 4522)
  AUTHORS   Tan,S., Wang,P., Hu,J., Wang,X. and Li,H.
  TITLE     NEDD9 Mediates the FAK/Src Signaling Pathway to Promote the
            Adhesion of Human Trabecular Meshwork Cells after Dexamethasone
            Treatment
  JOURNAL   Curr Eye Res 47 (8), 1156-1164 (2022)
   PUBMED   35577404
  REMARK    GeneRIF: NEDD9 Mediates the FAK/Src Signaling Pathway to Promote
            the Adhesion of Human Trabecular Meshwork Cells after Dexamethasone
            Treatment.
REFERENCE   5  (bases 1 to 4522)
  AUTHORS   de Jong,R., van Wijk,A., Haataja,L., Heisterkamp,N. and Groffen,J.
  TITLE     BCR/ABL-induced leukemogenesis causes phosphorylation of Hef1 and
            its association with Crkl
  JOURNAL   J Biol Chem 272 (51), 32649-32655 (1997)
   PUBMED   9405482
REFERENCE   6  (bases 1 to 4522)
  AUTHORS   Hunter,A.J. and Shimizu,Y.
  TITLE     Alpha 4 beta 1 integrin-mediated tyrosine phosphorylation in human
            T cells: characterization of Crk- and Fyn-associated substrates
            (pp105, pp115, and human enhancer of filamentation-1) and
            integrin-dependent activation of p59fyn1
  JOURNAL   J Immunol 159 (10), 4806-4814 (1997)
   PUBMED   9366405
REFERENCE   7  (bases 1 to 4522)
  AUTHORS   Tachibana,K., Urano,T., Fujita,H., Ohashi,Y., Kamiguchi,K.,
            Iwata,S., Hirai,H. and Morimoto,C.
  TITLE     Tyrosine phosphorylation of Crk-associated substrates by focal
            adhesion kinase. A putative mechanism for the integrin-mediated
            tyrosine phosphorylation of Crk-associated substrates
  JOURNAL   J Biol Chem 272 (46), 29083-29090 (1997)
   PUBMED   9360983
REFERENCE   8  (bases 1 to 4522)
  AUTHORS   Manie,S.N., Beck,A.R., Astier,A., Law,S.F., Canty,T., Hirai,H.,
            Druker,B.J., Avraham,H., Haghayeghi,N., Sattler,M., Salgia,R.,
            Griffin,J.D., Golemis,E.A. and Freedman,A.S.
  TITLE     Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking
            protein, in a cytoskeleton-dependent signaling pathway initiated by
            ligation of integrin or antigen receptor on human B cells
  JOURNAL   J Biol Chem 272 (7), 4230-4236 (1997)
   PUBMED   9020138
REFERENCE   9  (bases 1 to 4522)
  AUTHORS   Minegishi,M., Tachibana,K., Sato,T., Iwata,S., Nojima,Y. and
            Morimoto,C.
  TITLE     Structure and function of Cas-L, a 105-kD Crk-associated
            substrate-related protein that is involved in beta 1
            integrin-mediated signaling in lymphocytes
  JOURNAL   J Exp Med 184 (4), 1365-1375 (1996)
   PUBMED   8879209
REFERENCE   10 (bases 1 to 4522)
  AUTHORS   Law,S.F., Estojak,J., Wang,B., Mysliwiec,T., Kruh,G. and
            Golemis,E.A.
  TITLE     Human enhancer of filamentation 1, a novel p130cas-like docking
            protein, associates with focal adhesion kinase and induces
            pseudohyphal growth in Saccharomyces cerevisiae
  JOURNAL   Mol Cell Biol 16 (7), 3327-3337 (1996)
   PUBMED   8668148
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA778416.1, BC040207.1,
            AK292682.1, AJ420493.1 and AA814628.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006403.3.
            
            Summary: The protein encoded by this gene is a member of the
            CRK-associated substrates family. Members of this family are
            adhesion docking molecules that mediate protein-protein
            interactions for signal transduction pathways. This protein is a
            focal adhesion protein that acts as a scaffold to regulate
            signaling complexes important in cell attachment, migration and
            invasion as well as apoptosis and the cell cycle. This protein has
            also been reported to have a role in cancer metastasis. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040207.1, L43821.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379446.10/ ENSP00000368759.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-557               DA778416.1         15-571
            558-2864            BC040207.1         558-2864
            2865-3002           AK292682.1         3074-3211
            3003-3013           BC040207.1         3001-3011
            3014-3185           AJ420493.1         727-898
            3186-4520           BC040207.1         3184-4518
            4521-4522           AA814628.1         15-16               c
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p24.2"
     gene            1..4522
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /note="neural precursor cell expressed, developmentally
                     down-regulated 9"
                     /db_xref="GeneID:4739"
                     /db_xref="HGNC:HGNC:7733"
                     /db_xref="MIM:602265"
     exon            1..165
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             154..2658
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cas-like docking; Cas scaffolding protein family member 2;
                     Enhancer of filamentation 1 p55; p130Cas-related protein;
                     Crk-associated substrate related protein Cas-L; renal
                     carcinoma antigen NY-REN-12; neural precursor cell
                     expressed developmentally down-regulated protein 9"
                     /codon_start=1
                     /product="enhancer of filamentation 1 isoform 1"
                     /protein_id="NP_006394.1"
                     /db_xref="CCDS:CCDS4520.1"
                     /db_xref="GeneID:4739"
                     /db_xref="HGNC:HGNC:7733"
                     /db_xref="MIM:602265"
                     /translation="MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWW
                     LCSLHGRQGIVPGNRVKLLIGPMQETASSHEQPASGLMQQTFGQQKLYQVPNPQAAPR
                     DTIYQVPPSYQNQGIYQVPTGHGTQEQEVYQVPPSVQRSIGGTSGPHVGKKVITPVRT
                     GHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPVFSVPVGEIKPQGVYDIPP
                     TKGVYAIPPSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLH
                     VKYNCDIPGAAEPVARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSE
                     KANPQERDGVYDVPLHNPPDAKGSRDLVDGINRLSFSSTGSTRSNMSTSSTSSKESSL
                     SASPAQDKRLFLDPDTAIERLQRLQQALEMGVSSLMALVTTDWRCYGYMERHINEIRT
                     AVDKVELFLKEYLHFVKGAVANAACLPELILHNKMKRELQRVEDSHQILSQTSHDLNE
                     CSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTINTNAEALFRPGPGSLHL
                     KNGPESIMNSTEYPHGGSQGQLLHPGDHKAQAHNKALPPGLSKEQAPDCSSSDGSERS
                     WMDDYDYVHLQGKEEFERQQKELLEKENIMKQNKMQLEHHQLSQFQLLEQEITKPVEN
                     DISKWKPSQSLPTTNSGVSAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPR
                     IFVAHSKFVILSAHKLVFIGDTLTRQVTAQDIRNKVMNSSNQLCEQLKTIVMATKMAA
                     LHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF"
     exon            166..612
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            613..714
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            715..816
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            817..2058
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..2148
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2149..4522
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2969..2974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /note="hexamer: ATTAAA"
     polyA_site      2995
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /note="major polyA site"
     regulatory      4495..4500
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /note="hexamer: ATTAAA"
     polyA_site      4522
                     /gene="NEDD9"
                     /gene_synonym="CAS-L; CAS2; CASL; CASS2; HEF1"
                     /note="major polyA site"
ORIGIN      
        1 agagacttga gggaggcgct gcgactgaca agcggctctg cccgggacct tctcgctttc
       61 atctagcgct gcactcaatg gaggggcggg caccgcagtg cttaatgctg tcttaactag
      121 tgtaggaaaa cggctcaacc caccgctgcc gaaatgaagt ataagaatct tatggcaagg
      181 gccttatatg acaatgtccc agagtgtgcc gaggaactgg cctttcgcaa gggagacatc
      241 ctgaccgtca tagagcagaa cacaggggga ctggaaggat ggtggctgtg ctcattacac
      301 ggtcggcaag gcattgtccc aggcaaccgg gtgaagcttc tgattggtcc catgcaggag
      361 actgcctcca gtcacgagca gcctgcctct ggactgatgc agcagacctt tggccaacag
      421 aagctctatc aagtgccaaa cccacaggct gctccccgag acaccatcta ccaagtgcca
      481 ccttcctacc aaaatcaggg aatttaccaa gtccccactg gccacggcac ccaagaacaa
      541 gaggtatatc aggtgccacc atcagtgcag agaagcattg ggggaaccag tgggccccac
      601 gtgggtaaaa aggtgataac ccccgtgagg acaggccatg gctacgtata cgagtaccca
      661 tccagatacc aaaaggacgt ctatgatatc cctccttctc ataccactca aggggtatac
      721 gacatccctc cctcatcagc aaaaggccct gtgttttcag ttccagtggg agagataaaa
      781 cctcaagggg tgtatgacat cccgcctaca aaaggggtat atgccattcc gccctctgct
      841 tgccgggatg aagcagggct tagggaaaaa gactatgact tcccccctcc catgagacaa
      901 gctggaaggc cggacctcag accggagggg gtttatgaca ttcctccaac ctgcaccaag
      961 ccagcaggga aggaccttca tgtaaaatac aactgtgaca ttccaggagc tgcagaaccg
     1021 gtggctcgaa ggcaccagag cctgtccccg aatcacccac ccccgcaact cggacagtca
     1081 gtgggctctc agaacgacgc atatgatgtc ccccgaggcg ttcagtttct tgagccacca
     1141 gcagaaacca gtgagaaagc aaacccccag gaaagggatg gtgtttatga tgtccctctg
     1201 cataacccgc cagatgctaa aggctctcgg gacttggtgg atgggatcaa ccgattgtct
     1261 ttctccagta caggcagcac ccggagtaac atgtccacgt cttccacctc ctccaaggag
     1321 tcctcactgt cagcctcccc agctcaggac aaaaggctct tcctggatcc agacacagct
     1381 attgagagac ttcagcggct ccagcaggcc cttgagatgg gtgtctccag cctaatggca
     1441 ctggtcacta ccgactggcg gtgttacgga tatatggaaa gacacatcaa tgaaatacgc
     1501 acagcagtgg acaaggtgga gctgttcctg aaggagtacc tccactttgt caagggagct
     1561 gttgcaaatg ctgcctgcct cccggaactc atcctccaca acaagatgaa gcgggagctg
     1621 caacgagttg aagactccca ccagatcctg agtcaaacca gccatgactt aaatgagtgc
     1681 agctggtccc tgaatatctt ggccatcaac aagccccaga acaagtgtga cgatctggac
     1741 cggtttgtga tggtggcaaa gacggtgccc gatgacgcca agcagctcac cacaaccatc
     1801 aacaccaacg cagaggccct cttcagaccc ggccctggca gcttgcatct gaagaatggg
     1861 ccggagagca tcatgaactc aacggagtac ccacacggtg gctcccaggg acagctgctg
     1921 catcctggtg accacaaggc ccaggcccac aacaaggcac tgcccccagg cctgagcaag
     1981 gagcaggccc ctgactgtag cagcagtgat ggttctgaga ggagctggat ggatgactac
     2041 gattacgtcc acctacaggg taaggaggag tttgagaggc aacagaaaga gctattggaa
     2101 aaagagaata tcatgaaaca gaacaagatg cagctggaac atcatcagct gagccagttc
     2161 cagctgttgg aacaagagat tacaaagccc gtggagaatg acatctcgaa gtggaagccc
     2221 tctcagagcc tacccaccac aaacagtggc gtgagtgctc aggatcggca gttgctgtgc
     2281 ttctactatg accaatgtga gacccatttc atttcccttc tcaacgccat tgacgcactc
     2341 ttcagttgtg tcagctcagc ccagcccccg cgaatcttcg tggcacacag caagtttgtc
     2401 atcctcagtg cacacaaact ggtgttcatt ggagacacgc tgacacggca ggtgactgcc
     2461 caggacattc gcaacaaagt catgaactcc agcaaccagc tctgcgagca gctcaagacc
     2521 atagtcatgg caaccaagat ggccgccctc cattacccca gcaccacggc cctgcaggaa
     2581 atggtgcacc aagtgacaga cctttctaga aatgcccagc tgttcaagcg ctctttgctg
     2641 gagatggcaa cgttctgaga agaaaaaaaa gaggaagggg actgcgttaa cggttactaa
     2701 ggaaaactgg aaatactgtc tggtttttgt aaatgttatc tatttttgta gatattttat
     2761 ataaaaatga aatattttaa cattttatgg gtcagtcaac tttcagaaat tcagggagct
     2821 ggagagggaa atcttttttt ttccccctga gtggttctta tgtatacata gaagtatctg
     2881 agacataaac tgtacagaaa acttgtccac gtgcttttgt atgcccatgt attcatgttt
     2941 gtttgtagat gtttgtctga tgcatttcat taaaaaaaaa accatgaatt acgaagcacc
     3001 ttagtaagca ccttctaatg ctgcattttt tttgttgttg ttaaaaacat accagctggt
     3061 tataatattg ttctccacgt ccttgtgatg attctgagcc tggcactccc aaatctggga
     3121 agcatagttt atttgcaagt gttcaccttc caaatcatga ggcatagcat gacttattct
     3181 tgtttggaaa actcttttca aaactgacca tcttaaacac atgatggcca agtgcccaaa
     3241 agccctcttg cggagcaaat ttcagaatat atatgtggat ccaagctctg atagttcagg
     3301 tgctggaggg aagagagacc tgtgtgttta gaggccagga ccacagttag gattgggttg
     3361 tttcaatact gagagacagc tacaataaaa ggagagcaat tgcctccctg gggctgttca
     3421 atcttctgca tttgtgagtg gttcagtcat gaggttttcc aaaagatgtt tttagagttg
     3481 taaaaaccat atttgcagca aagatttaca aaggcgtatc agactatgat tgttcaccaa
     3541 aataggggaa tggtttgatc cgccagttgc aagtagaggc ctttctgact cttaatattc
     3601 actttggtgc tactaccccc attacctgag ggaaactggc caggtccttg atcatggaac
     3661 tatagagcta ccaggacata tcctgctctc taagggaatt tattgctatc ttgcaccttc
     3721 tttaaaactc acatatgcag acctgacact caagagtggc tagctacaca gagtccatct
     3781 aatttttgca acttcctgtg gccagtgtgt ataacccctt ccactatctc acagatagtc
     3841 acagcgtcca ttccatagtc tgtctcctca catctgttag tattgacaca gcacagacac
     3901 cacaagccat caggttcttc atggggcagg tgaaatactt ctaccccatg ggtaaatgta
     3961 ttcacatatt accaagagaa gaagcacatt atctatgatc ttttggccca gttcttattt
     4021 agcattttta ttccagccta cttggaaaca tgtttttatt tgcaatatat gcctgactga
     4081 attaagcttg cttgttttaa acaaccaaat cattggaaca gaaaaggatt taaaaaacaa
     4141 gaatgcatga tctcagagtg attaaaaaaa aatcagtgga aataaatgat catagaaggt
     4201 gcttttcaaa acaactgcta ttataattct caaagtccta ctctgccaaa agaagattaa
     4261 aagtcataca ttacattaca aggaaatgtt catgtgggaa gagggttgct gaaaatcaac
     4321 aacgcttgaa gttaaaaagt gtgtctttgt agatttcatt gtataatgtg tatttcttag
     4381 gagatggctg acttgattga tctacgctaa gtggagacat ttcacatttt taaaaccaaa
     4441 tgttcaatct gtattactct ttgccgtctt gtatgtagag gctattttta aatcattaaa
     4501 tttttagatc tctgttttca ta
//
