LOCUS       NM_006154               5735 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript
            variant 1, mRNA.
ACCESSION   NM_006154
VERSION     NM_006154.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Ling,X., Xu,W., Tang,J., Cao,Q., Luo,G., Chen,X., Yang,S.,
            Reinach,P.S. and Yan,D.
  TITLE     The Role of Ubiquitination and the E3 Ligase Nedd4 in Regulating
            Corneal Epithelial Wound Healing
  JOURNAL   Invest Ophthalmol Vis Sci 65 (6), 29 (2024)
   PUBMED   38888282
  REMARK    GeneRIF: The Role of Ubiquitination and the E3 Ligase Nedd4 in
            Regulating Corneal Epithelial Wound Healing.
REFERENCE   3  (bases 1 to 5735)
  AUTHORS   Hu,H., Ma,J., Peng,Y., Feng,R., Luo,C., Zhang,M., Tao,Z., Chen,L.,
            Zhang,T., Chen,W., Yin,Q., Zhai,J., Chen,J., Yin,A., Wang,C.C. and
            Zhong,M.
  TITLE     Thrombospondin-1 Regulates Trophoblast Necroptosis via
            NEDD4-Mediated Ubiquitination of TAK1 in Preeclampsia
  JOURNAL   Adv Sci (Weinh) 11 (21), e2309002 (2024)
   PUBMED   38569496
  REMARK    GeneRIF: Thrombospondin-1 Regulates Trophoblast Necroptosis via
            NEDD4-Mediated Ubiquitination of TAK1 in Preeclampsia.
REFERENCE   4  (bases 1 to 5735)
  AUTHORS   He,J., Yi,J., Ji,L., Dai,L., Chen,Y. and Xue,W.
  TITLE     ECHDC2 inhibits the proliferation of gastric cancer cells by
            binding with NEDD4 to degrade MCCC2 and reduce aerobic glycolysis
  JOURNAL   Mol Med 30 (1), 69 (2024)
   PUBMED   38783226
  REMARK    GeneRIF: ECHDC2 inhibits the proliferation of gastric cancer cells
            by binding with NEDD4 to degrade MCCC2 and reduce aerobic
            glycolysis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5735)
  AUTHORS   Lv,B., Fu,P., Wang,M., Cui,L., Bao,L., Wang,X., Yu,L., Zhou,C.,
            Zhu,M., Wang,F., Pang,Y., Qi,S., Zhang,Z. and Cui,G.
  TITLE     DMT1 ubiquitination by Nedd4 protects against ferroptosis after
            intracerebral hemorrhage
  JOURNAL   CNS Neurosci Ther 30 (4), e14685 (2024)
   PUBMED   38634270
  REMARK    GeneRIF: DMT1 ubiquitination by Nedd4 protects against ferroptosis
            after intracerebral hemorrhage.
REFERENCE   6  (bases 1 to 5735)
  AUTHORS   Staub,O., Gautschi,I., Ishikawa,T., Breitschopf,K., Ciechanover,A.,
            Schild,L. and Rotin,D.
  TITLE     Regulation of stability and function of the epithelial Na+ channel
            (ENaC) by ubiquitination
  JOURNAL   EMBO J 16 (21), 6325-6336 (1997)
   PUBMED   9351815
REFERENCE   7  (bases 1 to 5735)
  AUTHORS   Gavva,N.R., Gavva,R., Ermekova,K., Sudol,M. and Shen,C.J.
  TITLE     Interaction of WW domains with hematopoietic transcription factor
            p45/NF-E2 and RNA polymerase II
  JOURNAL   J Biol Chem 272 (39), 24105-24108 (1997)
   PUBMED   9305852
REFERENCE   8  (bases 1 to 5735)
  AUTHORS   Hatakeyama,S., Jensen,J.P. and Weissman,A.M.
  TITLE     Subcellular localization and ubiquitin-conjugating enzyme (E2)
            interactions of mammalian HECT family ubiquitin protein ligases
  JOURNAL   J Biol Chem 272 (24), 15085-15092 (1997)
   PUBMED   9182527
REFERENCE   9  (bases 1 to 5735)
  AUTHORS   Kumar,S., Harvey,K.F., Kinoshita,M., Copeland,N.G., Noda,M. and
            Jenkins,N.A.
  TITLE     cDNA cloning, expression analysis, and mapping of the mouse Nedd4
            gene
  JOURNAL   Genomics 40 (3), 435-443 (1997)
   PUBMED   9073511
  REMARK    Erratum:[Genomics 1997 Aug 15;44(1):156]
REFERENCE   10 (bases 1 to 5735)
  AUTHORS   Imhof,M.O. and McDonnell,D.P.
  TITLE     Yeast RSP5 and its human homolog hRPF1 potentiate hormone-dependent
            activation of transcription by human progesterone and
            glucocorticoid receptors
  JOURNAL   Mol Cell Biol 16 (6), 2594-2605 (1996)
   PUBMED   8649367
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D42055.1, AL832063.1,
            AL833300.1 and AC009997.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_006154.3.
            
            Summary: This gene is the founding member of the NEDD4 family of
            HECT ubiquitin ligases that function in the ubiquitin proteasome
            system of protein degradation. The encoded protein contains an
            N-terminal calcium and phospholipid binding C2 domain followed by
            multiple tryptophan-rich WW domains and, a C-terminal HECT
            ubiquitin ligase catalytic domain. It plays critical role in the
            regulation of a number of membrane receptors, endocytic machinery
            components and the tumor suppressor PTEN. [provided by RefSeq, Jul
            2016].
            
            Transcript Variant: This variant (1) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and contains multiple alternate 5'
            coding exons, compared to variant 2. It represents use of an
            alternate promoter and initiates translation at an alternate start
            codon. The encoded isoform (1) is shorter and has a distinct
            N-terminus, compared to isoform 2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC152562.1, D42055.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000435532.8/ ENSP00000410613.3
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-835               D42055.1           26-860
            836-1083            AL832063.1         2121-2368
            1084-2584           D42055.1           1109-2609
            2585-2657           AL833300.1         3067-3139
            2658-5735           AC009997.7         122042-125119       c
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.3"
     gene            1..5735
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="NEDD4 E3 ubiquitin protein ligase"
                     /db_xref="GeneID:4734"
                     /db_xref="HGNC:HGNC:7727"
                     /db_xref="MIM:602278"
     exon            1..102
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..2760
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1;
                     receptor-potentiating factor 1; cell
                     proliferation-inducing gene 53 protein; neural precursor
                     cell expressed, developmentally down-regulated 4, E3
                     ubiquitin protein ligase; HECT-type E3 ubiquitin
                     transferase NEDD4"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase NEDD4 isoform 1"
                     /protein_id="NP_006145.2"
                     /db_xref="CCDS:CCDS45265.1"
                     /db_xref="GeneID:4734"
                     /db_xref="HGNC:HGNC:7727"
                     /db_xref="MIM:602278"
                     /translation="MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYV
                     RVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDF
                     LGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDD
                     NAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQ
                     WKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATM
                     YSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTF
                     EEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTS
                     SQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWE
                     DPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLEN
                     VAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRA
                     DFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNS
                     GLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYY
                     NSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWR
                     FVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGY
                     SANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWG
                     TPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD"
     exon            103..176
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            177..255
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            256..294
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            295..348
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            349..399
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            400..461
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            462..564
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..731
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            732..849
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            850..1017
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1083
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1203
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1404
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1485
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1540
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1599
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1665
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1895
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2017
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2088
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2184
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2185..2258
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2319
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2379
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2487
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2584
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2585..2657
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..5735
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3236..3241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: AGTAAA"
     polyA_site      3261
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="major polyA site"
     regulatory      4279..4284
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: ATTAAA"
     polyA_site      4303
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="major polyA site"
     regulatory      4352..4357
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: AATATA"
     polyA_site      4389
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="major polyA site"
     regulatory      5134..5139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: AATACA"
     polyA_site      5153
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
     regulatory      5444..5449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: ATTAAA"
     regulatory      5457..5462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: AACAAA"
     polyA_site      5463
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
     polyA_site      5475
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
     regulatory      5617..5622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
                     /note="hexamer: AGTAAA"
     polyA_site      5636
                     /gene="NEDD4"
                     /gene_synonym="NEDD4-1; RPF1"
ORIGIN      
        1 acagttgcct gccctgggcg ggggcgagcg cgtccggttt gctggaagcg ttcggaaatg
       61 gcaacttgcg cggtggaggt gttcgggctc ctggaggacg aggaaaattc acgaattgtg
      121 agagtaagag ttatagccgg aataggcctt gccaagaagg atatattggg agctagtgat
      181 ccttacgtga gagtgacgtt atatgaccca atgaatggag ttcttacaag tgtgcaaaca
      241 aaaaccatta aaaagagttt gaatccaaag tggaatgaag aaatattatt cagagttcat
      301 cctcagcagc accggcttct ttttgaagtg tttgacgaaa accgattgac aagagatgat
      361 ttcctaggtc aagtggatgt tccactttat ccattaccga cagaaaatcc aagattggag
      421 agaccatata catttaagga ttttgttctt catccaagaa gtcacaaatc aagagttaaa
      481 ggttatctga gactaaaaat gacttattta cctaaaacca gtggctcaga agatgataat
      541 gcagaacagg ctgaggaatt agagcctggc tgggttgttt tggaccaacc agatgctgct
      601 tgccatttgc agcaacaaca agaaccttct cctctacctc cagggtggga agagaggcag
      661 gatatccttg gaaggaccta ttatgtaaac catgaatcta gaagaacaca gtggaaaaga
      721 ccaacccctc aggacaacct aacagatgct gagaatggca acattcaact gcaagcacaa
      781 cgtgcattta ccaccaggcg gcagatatcc gaggaaacag aaagtgttga caaccgagag
      841 tcttccgaga actgggaaat tataagagaa gatgaagcca ccatgtatag caaccaggcc
      901 ttcccatcac ctccaccgtc aagtaacttg gatgttccaa ctcatcttgc agaagaattg
      961 aatgccagac tcaccatttt tggaaattca gccgtgagcc agccagcatc gagctcaaat
     1021 cattccagca gaagaggcag cttacaagcc tatacttttg aggaacaacc tacacttcct
     1081 gtgcttttgc ctacttcatc tggattacca ccaggttggg aagaaaaaca agatgaaaga
     1141 ggaagatcat attatgtaga tcacaattcc agaacgacta cttggacaaa gcccactgta
     1201 caggccacag tggagaccag tcagctgacc tcaagccaga gttctgcagg ccctcaatca
     1261 caagcctcca ccagtgattc aggccagcag gtgacccagc catctgaaat tgagcaagga
     1321 ttccttccta aaggctggga agtccggcat gcaccaaatg ggaggccttt ctttattgac
     1381 cacaacacta aaaccaccac ctgggaagat ccaagattga aaattccagc ccatctgaga
     1441 ggaaagacat cacttgatac ttccaatgat ctagggcctt tacctccagg atgggaagag
     1501 agaactcaca cagatggaag aatcttctac ataaatcaca atataaaaag aacacaatgg
     1561 gaagatcctc ggttggagaa tgtagcaata actggaccag cagtgcccta ctccagggat
     1621 tacaaaagaa agtatgagtt cttccgaaga aagttgaaga agcagaatga cattccaaac
     1681 aaatttgaaa tgaaacttcg ccgagcaact gttcttgaag actcttaccg gagaattatg
     1741 ggtgtcaaga gagcagactt cctgaaggct cgactgtgga ttgagtttga tggtgaaaag
     1801 ggattggatt atggaggagt tgccagagaa tggttcttcc tgatctcaaa ggaaatgttt
     1861 aacccttatt atgggttgtt tgaatattct gctacggaca attataccct acagataaat
     1921 ccaaactctg gattgtgtaa cgaagatcac ctctcttact tcaagtttat tggtcgggta
     1981 gctggaatgg cagtttatca tggcaaactg ttggatggtt ttttcatccg cccattttac
     2041 aagatgatgc ttcacaaacc aataaccctt catgatatgg aatctgtgga tagtgaatat
     2101 tacaattccc taagatggat tcttgaaaat gacccaacag aattggacct caggtttatc
     2161 atagatgaag aactttttgg acagacacat caacatgagc tgaaaaatgg tggatcagaa
     2221 atagttgtca ccaataagaa caaaaaggaa tatatttatc ttgtaataca atggcgattt
     2281 gtaaaccgaa tccagaagca aatggctgct tttaaagagg gattctttga actaatacca
     2341 caggatctca tcaaaatttt tgatgaaaat gaactagagc ttcttatgtg tggactggga
     2401 gatgttgatg tgaatgactg gagggaacat acaaagtata aaaatggcta cagtgcaaat
     2461 catcaggtta tacagtggtt ttggaaggct gttttaatga tggattcaga aaaaagaata
     2521 agattacttc agtttgtcac tggcacatct cgggtgccta tgaatggatt tgctgaacta
     2581 tacggttcaa atggaccaca gtcatttaca gttgaacagt ggggtactcc tgaaaagctg
     2641 ccaagagctc atacctgttt taatcgcctg gacttgccac cttatgaatc atttgaagaa
     2701 ttatgggata aacttcagat ggcaattgaa aacacccagg gctttgatgg agttgattag
     2761 attacaaata acaatctgta gtgtttttac tgccatagtt ttataaccaa aatcttgact
     2821 taaaattttc cggggaacta ctaaaatgtg gccactgagt cttcccagat cttgaagaaa
     2881 atcatataaa aagcatttga agaaatagta cgacaactta tttttaatca cttttaaata
     2941 atgtgttgca tttacacagt tgtttcatgc tgtctttaga gttaggtgcc tgcctaaagc
     3001 caggcaccac cacacctggc tttagagttc acacaatagg atataagtcc tgtatgactt
     3061 aaatagtgaa ttttgtcctt aacatttacc tcttgtatag tatctgccag gcagtttttt
     3121 cttaaactac tgagatgata actgtgaaat atttgtgata cgtgtcatgt gtgaaaagtt
     3181 tgatgcattt tgagatggaa aactgaaatt tggaaaaaga aatactttac tattgagtaa
     3241 actacaatat atttagtgct actcgcagct atttattatt ttgtagacct gccttatgca
     3301 ccttactgcc tagatttttg ggaaaaaact ttggaaagtg tgttacctat atttctagcc
     3361 aactaactca cagaaaaact gtttacttct tcactttcga agtatttggc ttttgttaat
     3421 atgcagtttt actaaacaga tggttcataa gacatgtgaa gcaaattcat atttgcaatg
     3481 ggtaaaaagt attaaagcct ttctcttgcc tgcatatcct attgaccatt ggtatagtca
     3541 tcactttttc atattttagt gtagttagaa gaattccttc ttcaaacatt aaagatccac
     3601 aaagcagtat ttctaaatat gccttgaaga actaaatgaa gtgtatagca cttgccttta
     3661 ctagatattc tttacacttg tacaattatg tagtaaatgt atgtttacag ggtttatcat
     3721 gtttacagat taagctaatt tctgtagtcg catttttata tttttagtat cactctagta
     3781 aaaaaaccaa ataatttgtt taaaataacc aaagagttgt tataatgcat aatttgtatt
     3841 aaatttatta ctatttctta tgccttttaa aatactgttt actatgaaga caatgttttt
     3901 aattacaaat ccagaattct gtaggcaaaa tgctacagtt catatcttcc tttaaccaaa
     3961 ctgaagtaca taaagaccat gtacatgtat tcatcaaacg tttattgaat gctgcgtgcc
     4021 tagcgctgtg ctatgctctg gggtaagagt tgtcagcttc agagaagctg agtcctgatc
     4081 ctcaaggaac ttgcaaatgt gtctatgaat ttgtaaaaca atcaaaagta ggcgtaagca
     4141 gaataaggca aaagggaaag tgttctaggt tccagcacac ctgcaaagat aaagtgtgcc
     4201 aagactgtat ttatatttca tacttatatt gtttcatcct tatattggaa tgattatata
     4261 gaaaatgctc ttaaaaagat taaacctatt tctcagtatg gtatcttggt gatttaggaa
     4321 taattgtaaa tatatgttac gaatcttctt aaatatatat atatacacac cctgtgagaa
     4381 ctgtaaaaag tacctctggt tcttggttta agtttgttgg ggtataacat gatgagtact
     4441 cattagcacc tgatagaaat ctgaaatgtg acagtagcaa aaccactttc tactttccaa
     4501 acaccacagc atcagcatgg tttaggggaa gcaattcaca gattaatgta ccctgcgttt
     4561 tgtcttcacc attgtcaacc agcagtcaag gatgagcacc aggtatagtc ctggtttgat
     4621 atctcaccag ccatgtgaac tgaagcagct tacttaacct ctctggattc atatttcttc
     4681 atctgtcaaa tgagagtaat catgcccacc ctcattgtca tgccagattg tcagaagttc
     4741 cagacagatg agaaagcaaa aagtactttg taaagtgtta tacaatcaga aggtgctttg
     4801 tgctgaccgt cagattgtgt caagtcaggt gtgcaaattg accacactcc caaagaatca
     4861 tttaaaagta aagtgacttc taagagacaa ggaagtagga acatttctga tattaaatat
     4921 ataaataata tggatacaca tgtgtatatc tgtatatgga tcttaccatc ataattcatt
     4981 ttcttcatat cagcaaagta atagtaatgt gggccaatgc attttggaaa tgtcctcatt
     5041 atgtagaatg gaatgtgaaa attatttttg ttaaaacagg attttgccac aattatttaa
     5101 atattatgtt tgtgaactat ctaagcatga gaaaatacaa agctttcttt gtatctggct
     5161 gatactcatt tggatgattc tgtgactcat agaacatgat gttaaatgag accagactca
     5221 actgccacca gtccccagct gcagaccttc atccccttca tctcccacta ggggctcgtg
     5281 gtctggaaga aacatctatc aagcaacaca gccccttatc ccagatagaa aagtgtctca
     5341 aatgcattct actgcgggac agtcagtagg atcattttca taaagcaagg acatcatatg
     5401 tttctagaaa ttacaagcat ataactttag cctacaatct cttattaaaa atttttaaca
     5461 aaattgtatt acaaatgcat ttcatcagaa ctcaaattta aatggtgttt gttttgggtt
     5521 tttatttata atgctgaagt tattccatat aagtatcaag ttaaacacaa ttcattttga
     5581 ttataaacta tttgactttt taaaatcttc tgacacagta aatatatata tcaagattga
     5641 tgtatcaaaa tttattgcac actttaaagt gtaaaatcat tttttaaaat cttgaatcca
     5701 caaataaagt tctattctga ttttaaaaaa caaaa
//
