LOCUS       NM_030916               3458 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens nectin cell adhesion molecule 4 (NECTIN4), mRNA.
ACCESSION   NM_030916
VERSION     NM_030916.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Dash,S., Biswas,J., Goswami,S., Mukherjee,S., Ganguli,N.,
            Duraivelan,K., Mondal,S., Mukhopadhyay,R. and Samanta,D.
  TITLE     Molecular Crosstalk Between Adherens Junction Proteins, E-cadherin
            and Nectin-4
  JOURNAL   J Mol Biol 436 (18), 168709 (2024)
   PUBMED   39009071
  REMARK    GeneRIF: Molecular Crosstalk Between Adherens Junction Proteins,
            E-cadherin and Nectin-4.
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Bahlinger,V., Branz,A., Strissel,P.L., Strick,R., Lange,F.,
            Geppert,C.I., Klumper,N., Holzel,M., Wach,S., Taubert,H., Sikic,D.,
            Wullich,B., Angeloni,M., Ferrazzi,F., Diehl,L., Kovalenko,M.,
            Elboudwarej,E., Jurgensmeier,J.M., Hartmann,A. and Eckstein,M.
  TITLE     Associations of TACSTD2/TROP2 and NECTIN-4/NECTIN-4 with molecular
            subtypes, PD-L1 expression, and FGFR3 mutational status in two
            advanced urothelial bladder cancer cohorts
  JOURNAL   Histopathology 84 (5), 863-876 (2024)
   PUBMED   38196202
  REMARK    GeneRIF: Associations of TACSTD2/TROP2 and NECTIN-4/NECTIN-4 with
            molecular subtypes, PD-L1 expression, and FGFR3 mutational status
            in two advanced urothelial bladder cancer cohorts.
REFERENCE   3  (bases 1 to 3458)
  AUTHORS   Nanamiya,T., Takane,K., Yamaguchi,K., Okawara,Y., Arakawa,M.,
            Saku,A., Ikenoue,T., Fujiyuki,T., Yoneda,M., Kai,C. and Furukawa,Y.
  TITLE     Expression of PVRL4, a molecular target for cancer treatment, is
            transcriptionally regulated by FOS
  JOURNAL   Oncol Rep 51 (1) (2024)
   PUBMED   38063270
  REMARK    GeneRIF: Expression of PVRL4, a molecular target for cancer
            treatment, is transcriptionally regulated by FOS.
REFERENCE   4  (bases 1 to 3458)
  AUTHORS   Katasho,R., Nagano,T., Iwasaki,T. and Kamada,S.
  TITLE     Nectin-4 regulates cellular senescence-associated enlargement of
            cell size
  JOURNAL   Sci Rep 13 (1), 21602 (2023)
   PUBMED   38062106
  REMARK    GeneRIF: Nectin-4 regulates cellular senescence-associated
            enlargement of cell size.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3458)
  AUTHORS   Ali,G., Sadia,S., Ain-Ul-Batool,S., Azeem,Z., Awan,N.B.,
            Kazmi,S.A.R., Ur-Rehman,Z., Anjum,Z., Ur-Rehman,F., Wali,A.,
            Khan,K., Zaman,N., Ayub,M., Sajid,M. and Hassan,N.
  TITLE     A Recurrent Nonsense Mutation in NECTIN4 Underlying Ectodermal
            Dysplasia-Syndactyly Syndrome with a Novel Phenotype in a
            Consanguineous Kashmiri Family
  JOURNAL   Genet Res (Camb) 2023, 9999660 (2023)
   PUBMED   37829154
  REMARK    GeneRIF: A Recurrent Nonsense Mutation in NECTIN4 Underlying
            Ectodermal Dysplasia-Syndactyly Syndrome with a Novel Phenotype in
            a Consanguineous Kashmiri Family.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3458)
  AUTHORS   Fabre-Lafay,S., Garrido-Urbani,S., Reymond,N., Goncalves,A.,
            Dubreuil,P. and Lopez,M.
  TITLE     Nectin-4, a new serological breast cancer marker, is a substrate
            for tumor necrosis factor-alpha-converting enzyme (TACE)/ADAM-17
  JOURNAL   J Biol Chem 280 (20), 19543-19550 (2005)
   PUBMED   15784625
  REMARK    GeneRIF: the active form of TACE is overexpressed in breast tumors
            and may indicate that TACE is responsible for Nectin-4 shedding not
            only in vitro but also in vivo
REFERENCE   7  (bases 1 to 3458)
  AUTHORS   Fabre,S., Reymond,N., Cocchi,F., Menotti,L., Dubreuil,P.,
            Campadelli-Fiume,G. and Lopez,M.
  TITLE     Prominent role of the Ig-like V domain in trans-interactions of
            nectins. Nectin3 and nectin 4 bind to the predicted C-C'-C'-D
            beta-strands of the nectin1 V domain
  JOURNAL   J Biol Chem 277 (30), 27006-27013 (2002)
   PUBMED   12011057
REFERENCE   8  (bases 1 to 3458)
  AUTHORS   Reymond,N., Fabre,S., Lecocq,E., Adelaide,J., Dubreuil,P. and
            Lopez,M.
  TITLE     Nectin4/PRR4, a new afadin-associated member of the nectin family
            that trans-interacts with nectin1/PRR1 through V domain interaction
  JOURNAL   J Biol Chem 276 (46), 43205-43215 (2001)
   PUBMED   11544254
REFERENCE   9  (bases 1 to 3458)
  AUTHORS   Takahashi,K., Nakanishi,H., Miyahara,M., Mandai,K., Satoh,K.,
            Satoh,A., Nishioka,H., Aoki,J., Nomoto,A., Mizoguchi,A. and
            Takai,Y.
  TITLE     Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited
            to cadherin-based adherens junctions through interaction with
            Afadin, a PDZ domain-containing protein
  JOURNAL   J Cell Biol 145 (3), 539-549 (1999)
   PUBMED   10225955
REFERENCE   10 (bases 1 to 3458)
  AUTHORS   Lopez,M., Aoubala,M., Jordier,F., Isnardon,D., Gomez,S. and
            Dubreuil,P.
  TITLE     The human poliovirus receptor related 2 protein is a new
            hematopoietic/endothelial homophilic adhesion molecule
  JOURNAL   Blood 92 (12), 4602-4611 (1998)
   PUBMED   9845526
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB223835.1, BC010423.1,
            BC013892.2 and CF529301.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_030916.2.
            
            Summary: This gene encodes a member of the nectin family. The
            encoded protein contains two immunoglobulin-like (Ig-like) C2-type
            domains and one Ig-like V-type domain. It is involved in cell
            adhesion through trans-homophilic and -heterophilic interactions.
            It is a single-pass type I membrane protein. The soluble form is
            produced by proteolytic cleavage at the cell surface by the
            metalloproteinase ADAM17/TACE. The secreted form is found in both
            breast tumor cell lines and breast tumor patients. Mutations in
            this gene are the cause of ectodermal dysplasia-syndactyly syndrome
            type 1, an autosomal recessive disorder. Alternatively spliced
            transcript variants have been found but the full-length nature of
            the variant has not been determined.[provided by RefSeq, Jan 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB105191.1, AK027753.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368012.4/ ENSP00000356991.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DB223835.1         38-52
            16-2619             BC010423.1         1-2604
            2620-3047           BC013892.2         1653-2080
            3048-3458           CF529301.1         26-436              c
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.3"
     gene            1..3458
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /note="nectin cell adhesion molecule 4"
                     /db_xref="GeneID:81607"
                     /db_xref="HGNC:HGNC:19688"
                     /db_xref="MIM:609607"
     exon            1..341
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     CDS             263..1795
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /note="nectin 4; poliovirus receptor-related 4; Ig
                     superfamily receptor LNIR; poliovirus receptor-related
                     protein 4"
                     /codon_start=1
                     /product="nectin-4 precursor"
                     /protein_id="NP_112178.2"
                     /db_xref="CCDS:CCDS1216.1"
                     /db_xref="GeneID:81607"
                     /db_xref="HGNC:HGNC:19688"
                     /db_xref="MIM:609607"
                     /translation="MPLSLGAEMWGPEAWLLLLLLLASFTGRCPAGELETSDVVTVVL
                     GQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPP
                     PPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALE
                     EGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNG
                     QPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQP
                     PPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLD
                     PQEDSGKQVDLVSASVVVVGVIAALLFCLLVVVVVLMSRYHRRKAQQMTQKYEEELTL
                     TRENSIRRLHSHHTDPRSQPEESVGLRAEGHPDSLKDNSSCSVMSEEPEGRSYSTLTT
                     VREIETQTELLSPGSGRAEEEEDQDEGIKQAMNHFVQENGTLRAKPTGNGIYINGRGH
                     LV"
     exon            342..701
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            702..992
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1113
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1262
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1419
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1495
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1570
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..3458
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3440..3445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /note="hexamer: AATAAA"
     polyA_site      3458
                     /gene="NECTIN4"
                     /gene_synonym="EDSS1; LNIR; nectin-4; PRR4; PVRL4"
                     /note="major polyA site"
ORIGIN      
        1 gccgtcgttg ttggccacag cgtgggaagc agctctgggg gagctcggag ctcccgatca
       61 cggcttcttg ggggtagcta cggctgggtg tgtagaacgg ggccggggct ggggctgggt
      121 cccctagtgg agacccaagt gcgagaggca agaactctgc agcttcctgc cttctgggtc
      181 agttccttat tcaagtctgc agccggctcc cagggagatc tcggtggaac ttcagaaacg
      241 ctgggcagtc tgcctttcaa ccatgcccct gtccctggga gccgagatgt gggggcctga
      301 ggcctggctg ctgctgctgc tactgctggc atcatttaca ggccggtgcc ccgcgggtga
      361 gctggagacc tcagacgtgg taactgtggt gctgggccag gacgcaaaac tgccctgctt
      421 ctaccgaggg gactccggcg agcaagtggg gcaagtggca tgggctcggg tggacgcggg
      481 cgaaggcgcc caggaactag cgctactgca ctccaaatac gggcttcatg tgagcccggc
      541 ttacgagggc cgcgtggagc agccgccgcc cccacgcaac cccctggacg gctcagtgct
      601 cctgcgcaac gcagtgcagg cggatgaggg cgagtacgag tgccgggtca gcaccttccc
      661 cgccggcagc ttccaggcgc ggctgcggct ccgagtgctg gtgcctcccc tgccctcact
      721 gaatcctggt ccagcactag aagagggcca gggcctgacc ctggcagcct cctgcacagc
      781 tgagggcagc ccagccccca gcgtgacctg ggacacggag gtcaaaggca caacgtccag
      841 ccgttccttc aagcactccc gctctgctgc cgtcacctca gagttccact tggtgcctag
      901 ccgcagcatg aatgggcagc cactgacttg tgtggtgtcc catcctggcc tgctccagga
      961 ccaaaggatc acccacatcc tccacgtgtc cttccttgct gaggcctctg tgaggggcct
     1021 tgaagaccaa aatctgtggc acattggcag agaaggagct atgctcaagt gcctgagtga
     1081 agggcagccc cctccctcat acaactggac acggctggat gggcctctgc ccagtggggt
     1141 acgagtggat ggggacactt tgggctttcc cccactgacc actgagcaca gcggcatcta
     1201 cgtctgccat gtcagcaatg agttctcctc aagggattct caggtcactg tggatgttct
     1261 tgacccccag gaagactctg ggaagcaggt ggacctagtg tcagcctcgg tggtggtggt
     1321 gggtgtgatc gccgcactct tgttctgcct tctggtggtg gtggtggtgc tcatgtcccg
     1381 ataccatcgg cgcaaggccc agcagatgac ccagaaatat gaggaggagc tgaccctgac
     1441 cagggagaac tccatccgga ggctgcattc ccatcacacg gaccccagga gccagccgga
     1501 ggagagtgta gggctgagag ccgagggcca ccctgatagt ctcaaggaca acagtagctg
     1561 ctctgtgatg agtgaagagc ccgagggccg cagttactcc acgctgacca cggtgaggga
     1621 gatagaaaca cagactgaac tgctgtctcc aggctctggg cgggccgagg aggaggaaga
     1681 tcaggatgaa ggcatcaaac aggccatgaa ccattttgtt caggagaatg ggaccctacg
     1741 ggccaagccc acgggcaatg gcatctacat caatgggcgg ggacacctgg tctgacccag
     1801 gcctgcctcc cttccctagg cctggctcct tctgttgaca tgggagattt tagctcatct
     1861 tgggggcctc cttaaacacc cccatttctt gcggaagatg ctccccatcc cactgactgc
     1921 ttgaccttta cctccaaccc ttctgttcat cgggagggct ccaccaattg agtctctccc
     1981 accatgcatg caggtcactg tgtgtgtgca tgtgtgcctg tgtgagtgtt gactgactgt
     2041 gtgtgtgtgg aggggtgact gtccgtggag gggtgactgt gtccgtggtg tgtattatgc
     2101 tgtcatatca gagtcaagtg aactgtggtg tatgtgccac gggatttgag tggttgcgtg
     2161 ggcaacactg tcagggtttg gcgtgtgtgt catgtggctg tgtgtgacct ctgcctgaaa
     2221 aagcaggtat tttctcagac cccagagcag tattaatgat gcagaggttg gaggagagag
     2281 gtggagactg tggctcagac ccaggtgtgc gggcatagct ggagctggaa tctgcctccg
     2341 gtgtgaggga acctgtctcc taccacttcg gagccatggg ggcaagtgtg aagcagccag
     2401 tccctgggtc agccagaggc ttgaactgtt acagaagccc tctgccctct ggtggcctct
     2461 gggcctgctg catgtacata ttttctgtaa atatacatgc gccgggagct tcttgcagga
     2521 atactgctcc gaatcacttt taattttttt cttttttttt tcttgccctt tccattagtt
     2581 gtatttttta tttattttta tttttatttt tttttagaga tggagtctca ctatgttgct
     2641 caggctggcc ttgaactcct gggctcaagc aatcctcctg cctcagcctc cctagtagct
     2701 gggactttaa gtgtacacca ctgtgcctgc tttgaatcct ttacgaagag aaaaaaaaaa
     2761 ttaaagaaag cctttagatt tatccaatgt ttactactgg gattgcttaa agtgaggccc
     2821 ctccaacacc agggggttaa ttcctgtgat tgtgaaaggg gctacttcca aggcatcttc
     2881 atgcaggcag ccccttggga gggcacctga gagctggtag agtctgaaat tagggatgtg
     2941 agcctcgtgg ttactgagta aggtaaaatt gcatccacca ttgtttgtga taccttaggg
     3001 aattgcttgg acctggtgac aagggctcct gttcaatagt ggtgttgggg agagagagag
     3061 cagtgattat agaccgagag agtaggagtt gaggtgaggt gaaggaggtg ctgggggtga
     3121 gaatgtcgcc tttccccctg ggttttggat cactaattca aggctcttct ggatgtttct
     3181 ctgggttggg gctggagttc aatgaggttt atttttagct ggcccaccca gatacactca
     3241 gccagaatac ctagatttag tacccaaact cttcttagtc tgaaatctgc tggatttctg
     3301 gcctaaggga gaggctccca tccttcgttc cccagccagc ctaggacttc gaatgtggag
     3361 cctgaagatc taagatccta acatgtacat tttatgtaaa tatgtgcata tttgtacata
     3421 aaatgatatt ctgtttttaa ataaacagac aaaacttg
//
