LOCUS       NM_002855               5956 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens nectin cell adhesion molecule 1 (NECTIN1), transcript
            variant 1, mRNA.
ACCESSION   NM_002855
VERSION     NM_002855.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Kite,J., Hill,M., Preston,N., Rubina,A., Kollnberger,S.,
            Wang,E.C.Y. and Elliott,G.
  TITLE     Downregulation of endogenous nectin1 in human keratinocytes by
            herpes simplex virus 1 glycoprotein D excludes superinfection but
            does not affect NK cell function
  JOURNAL   J Gen Virol 105 (3) (2024)
   PUBMED   38471041
  REMARK    GeneRIF: Downregulation of endogenous nectin1 in human
            keratinocytes by herpes simplex virus 1 glycoprotein D excludes
            superinfection but does not affect NK cell function.
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Park,J.W., Kang,G., Baek,S.H. and Kim,Y.H.
  TITLE     Genetic associations and parent-of-origin effects of PVRL1 in
            non-syndromic cleft lip with or without cleft palate across
            multiple ethnic populations
  JOURNAL   Epidemiol Health 46, e2024069 (2024)
   PUBMED   39139080
  REMARK    GeneRIF: Genetic associations and parent-of-origin effects of PVRL1
            in non-syndromic cleft lip with or without cleft palate across
            multiple ethnic populations.
REFERENCE   3  (bases 1 to 5956)
  AUTHORS   De La Cruz,N.C., Mockel,M., Niehues,H., Rubsam,M., Malter,W.,
            Zinser,M., Krummenacher,C. and Knebel-Morsdorf,D.
  TITLE     Ex Vivo Infection of Human Skin Models with Herpes Simplex Virus 1:
            Accessibility of the Receptor Nectin-1 during Formation or
            Impairment of Epidermal Barriers Is Restricted by Tight Junctions
  JOURNAL   J Virol 97 (6), e0026223 (2023)
   PUBMED   37289055
  REMARK    GeneRIF: Ex Vivo Infection of Human Skin Models with Herpes Simplex
            Virus 1: Accessibility of the Receptor Nectin-1 during Formation or
            Impairment of Epidermal Barriers Is Restricted by Tight Junctions.
REFERENCE   4  (bases 1 to 5956)
  AUTHORS   Ablain,J., Al Mahi,A., Rothschild,H., Prasad,M., Aires,S., Yang,S.,
            Dokukin,M.E., Xu,S., Dang,M., Sokolov,I., Lian,C.G. and Zon,L.I.
  TITLE     Loss of NECTIN1 triggers melanoma dissemination upon local IGF1
            depletion
  JOURNAL   Nat Genet 54 (12), 1839-1852 (2022)
   PUBMED   36229674
  REMARK    GeneRIF: Loss of NECTIN1 triggers melanoma dissemination upon local
            IGF1 depletion.
REFERENCE   5  (bases 1 to 5956)
  AUTHORS   Kite,J., Russell,T., Jones,J. and Elliott,G.
  TITLE     Cell-to-cell transmission of HSV1 in human keratinocytes in the
            absence of the major entry receptor, nectin1
  JOURNAL   PLoS Pathog 17 (9), e1009631 (2021)
   PUBMED   34587223
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5956)
  AUTHORS   Krummenacher,C., Nicola,A.V., Whitbeck,J.C., Lou,H., Hou,W.,
            Lambris,J.D., Geraghty,R.J., Spear,P.G., Cohen,G.H. and
            Eisenberg,R.J.
  TITLE     Herpes simplex virus glycoprotein D can bind to poliovirus
            receptor-related protein 1 or herpesvirus entry mediator, two
            structurally unrelated mediators of virus entry
  JOURNAL   J Virol 72 (9), 7064-7074 (1998)
   PUBMED   9696799
REFERENCE   7  (bases 1 to 5956)
  AUTHORS   Geraghty,R.J., Krummenacher,C., Cohen,G.H., Eisenberg,R.J. and
            Spear,P.G.
  TITLE     Entry of alphaherpesviruses mediated by poliovirus receptor-related
            protein 1 and poliovirus receptor
  JOURNAL   Science 280 (5369), 1618-1620 (1998)
   PUBMED   9616127
REFERENCE   8  (bases 1 to 5956)
  AUTHORS   Lopez,M., Eberle,F., Mattei,M.G., Gabert,J., Birg,F., Bardin,F.,
            Maroc,C. and Dubreuil,P.
  TITLE     Complementary DNA characterization and chromosomal localization of
            a human gene related to the poliovirus receptor-encoding gene
  JOURNAL   Gene 155 (2), 261-265 (1995)
   PUBMED   7721102
REFERENCE   9  (bases 1 to 5956)
  AUTHORS   Bustos,T., Simosa,V., Pinto-Cisternas,J., Abramovits,W., Jolay,L.,
            Rodriguez,L., Fernandez,L. and Ramela,M.
  TITLE     Autosomal recessive ectodermal dysplasia: I. An undescribed
            dysplasia/malformation syndrome
  JOURNAL   Am J Med Genet 41 (4), 398-404 (1991)
   PUBMED   1776626
REFERENCE   10 (bases 1 to 5956)
  AUTHORS   Carritt,B. and Goldfarb,P.
  TITLE     A human chromosomal determinant for susceptibility to herpes
            simplex virus
  JOURNAL   Nature 264 (5586), 556-558 (1976)
   PUBMED   1034214
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP003390.1, AI819997.1,
            BC104948.1, AP003393.1 and BC021976.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002855.4.
            
            Summary: This gene encodes an adhesion protein that plays a role in
            the organization of adherens junctions and tight junctions in
            epithelial and endothelial cells. The protein is a
            calcium(2+)-independent cell-cell adhesion molecule that belongs to
            the immunoglobulin superfamily and has 3 extracellular
            immunoglobulin-like loops, a single transmembrane domain (in some
            isoforms), and a cytoplasmic region. This protein acts as a
            receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2
            (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral
            entry into epithelial and neuronal cells. Mutations in this gene
            cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1)
            as well as non-syndromic cleft lip with or without cleft palate
            (CL/P). Alternative splicing results in multiple transcript
            variants encoding proteins with distinct C-termini. [provided by
            RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest protein (isoform 1; also known
            as isoform Delta, alpha, or HveC).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF060231.1, BC104948.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267784 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264025.8/ ENSP00000264025.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-475               AP003390.1         31215-31689         c
            476-569             AI819997.1         1-94
            570-2242            BC104948.1         1-1673
            2243-5796           AP003393.1         86720-90273         c
            5797-5956           BC021976.2         927-1086
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..5956
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /note="nectin cell adhesion molecule 1"
                     /db_xref="GeneID:5818"
                     /db_xref="HGNC:HGNC:9706"
                     /db_xref="MIM:600644"
     exon            1..726
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /inference="alignment:Splign:2.1.0"
     CDS             648..2201
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; nectin 1; poliovirus receptor-related 1
                     (herpesvirus entry mediator C); ectodermal dysplasia 4
                     (Margarita Island type); poliovirus receptor-like 1;
                     poliovirus receptor-related protein 1; herpesvirus Ig-like
                     receptor; herpes virus entry mediator C; herpes simplex
                     virus type 1 sensitivity"
                     /codon_start=1
                     /product="nectin-1 isoform 1 precursor"
                     /protein_id="NP_002846.3"
                     /db_xref="CCDS:CCDS8426.1"
                     /db_xref="GeneID:5818"
                     /db_xref="HGNC:HGNC:9706"
                     /db_xref="MIM:600644"
                     /translation="MARMGLAGAAGRWWGLALGLTAFFLPGVHSQVVQVNDSMYGFIG
                     TDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRERVEFLRP
                     SFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAK
                     KGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSRE
                     AHQQSLACIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPP
                     ATEYHWTTLNGSLPKGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNIT
                     EFPYTPSPPEHGRRAGPVPTAIIGGVAGSILLVLIVVGGIVVALRRRRHTFKGDYSTK
                     KHVYGNGYSKAGIPQHHPPMAQNLQYPDDSDDEKKAGPLGGSSYEEEEEEEEGGGGGE
                     RKVGGPHPKYDEDAKRPYFTVDEAEARQDGYGDRTLGYQYDPEQLDLAENMVSQNDGS
                     FISKKEWYV"
     exon            727..1077
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1380
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1498
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1650
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..5956
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5937..5942
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /note="hexamer: AATAAA"
     polyA_site      5956
                     /gene="NECTIN1"
                     /gene_synonym="CD111; CLPED1; ED4; HIgR; HV1S; HVEC;
                     nectin-1; OFC7; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12"
                     /note="major polyA site"
ORIGIN      
        1 aaagagagcg agagcagcga gcgcggctcc acattgttgc ggatcgccgg cacccggcag
       61 agcggcggcg gctgggacgc gcggcgcctc cgacccgttc tcctcgcgcc cggccgcgca
      121 gccagagcca cccgggccgc cgaccgccga gccccgcgcc cgccgcctgg gccccgagcc
      181 ttctgcgccg cccgggtgcg tcccgccacc ctcggaggac ggccggccat ggacgcctgc
      241 aagttggagc cgagcgggag ggtgtgagcg ggccggggcc aggagcccgc gccgcgcaac
      301 cgggcagccg ggcgcgccgg gggtgggtcc ctctccccag ccccgctctg cgtggaagaa
      361 gagggcgggg accggcgccg ggaggagagc ggaggaggcg aaggggcatg actcgtgcaa
      421 cttgcggcgg gcatctgccg agcctctgag ccggcggcgg cccggggccc ggactgcggc
      481 cgcgcggatc ccacccagcc caccccgccc cggccgacgg ctgcagctga cctggatcct
      541 tcgagcgccc gccgaccgcc agcgatcttc cctcatcttc cgggctggtt tctgctgcgc
      601 gaggagcgct gccctcgccg cccctctcgc cggacccccg gcccccgatg gctcggatgg
      661 ggcttgcggg cgccgctgga cgctggtggg gactcgctct cggcttgacc gcattcttcc
      721 tcccaggcgt ccactcccag gtggtccagg tgaacgactc catgtatggc ttcatcggca
      781 cagacgtggt tctgcactgc agctttgcca acccgcttcc cagcgtgaag atcacccagg
      841 tcacatggca gaagtccacc aatggctcca agcagaacgt ggccatctac aacccatcca
      901 tgggcgtgtc cgtgctggct ccctaccgcg agcgtgtgga attcctgcgg ccctccttca
      961 ccgatggcac tatccgcctc tcccgcctgg agctggagga tgagggtgtc tacatctgcg
     1021 agtttgctac cttccctacg ggcaatcgag aaagccagct caatctcacg gtgatggcca
     1081 aacccaccaa ttggatagag ggtacccagg cagtgcttcg agccaagaag gggcaggatg
     1141 acaaggtcct ggtggccacc tgcacctcag ccaatgggaa gcctcccagt gtggtatcct
     1201 gggaaactcg gttaaaaggt gaggcagagt accaggagat ccggaacccc aatggcacag
     1261 tgacggtcat cagccgctac cgcctggtgc ccagcaggga agcccaccag cagtccttgg
     1321 cctgcatcgt caactaccac atggaccgct tcaaggaaag cctcactctc aacgtgcagt
     1381 atgagcctga ggtaaccatt gaggggtttg atggcaactg gtacctgcag cggatggacg
     1441 tgaagctcac ctgcaaagct gatgctaacc ccccagccac tgagtaccac tggaccacgc
     1501 taaatggctc tctccccaag ggtgtggagg cccagaacag aaccctcttc ttcaagggac
     1561 ccatcaacta cagcctggca gggacctaca tctgtgaggc caccaacccc atcggtacac
     1621 gctcaggcca ggtggaggtc aatatcacag aattccccta caccccgtct cctcccgaac
     1681 atgggcggcg cgccgggccg gtgcccacgg ccatcattgg gggcgtggcg gggagcatcc
     1741 tgctggtgtt gattgtggtc ggcgggatcg tggtcgccct gcgtcggcgc cggcacacct
     1801 tcaagggtga ctacagcacc aagaagcacg tgtatggcaa cggctacagc aaggcaggca
     1861 tcccccagca ccacccacca atggcacaga acctgcagta ccccgacgac tcagacgacg
     1921 agaagaaggc cggcccactg ggtggaagca gctatgagga ggaggaggag gaggaggagg
     1981 gcggtggagg gggcgagcgc aaggtgggcg gcccccaccc caaatatgac gaggacgcca
     2041 agcggcccta cttcaccgtg gatgaggccg aggcccgtca ggacggctac ggggaccgga
     2101 ctctgggcta ccagtacgac cctgagcagc tggacttggc tgagaacatg gtttctcaga
     2161 acgacgggtc tttcatttcc aagaaggagt ggtacgtgta gccccccttc cagagcctct
     2221 gtctgtgacc gctcctcccc agcccctccc cgcacgcccc ctgcccaccc cccacctccc
     2281 actccaggag ctgaacagag acttgcccag ctgcccaaag ccagccccga actcctgggg
     2341 ggccagggga gcccagggca gccacgactt ggctttgtgt tttatttcct ccctctcctc
     2401 cgcccttccc catggtgttg tgttcgactg tggctttagt gttactgtat cttcccttta
     2461 cgggccccac ctgcctgccc tctgtgtagg gagccctgtt ctcgtcttgt gtacctgggc
     2521 atccctccag ggtttgggga gccagcctcc cccgcccccc tacactggaa tttgtttgtg
     2581 tttcttcact gggggtggag ggctcaaggg gctctaagag ggaaaagctc cccacacccc
     2641 cagatgctgc tcctcctccc ggctagaggg aacccaacac ccaccagtga aatctgatgc
     2701 agctgggagt ttggaccttg ggtctagaca gctggtgcgc ccaagaggta gggggagggg
     2761 tgcggcttct ccccagttcc cagggagtct tgtttgttaa gcggggcctt gggcagtgtt
     2821 ttcctgctga gggtggtttt acattttttt tcctcattgg cccacagaga gtggagttgg
     2881 cctagctctg acgcgtcccc tctttatccc caatgtgccc cctccattag gggctccagg
     2941 tgggctccag cccacacttg ctctctgcct ctgctgacac gtgcacacac acacacatgc
     3001 acacactgct cctctctgcc tgctttcccc tggagtccca tgccctgccc catccagaca
     3061 ctgcccaagg cccacccgtc tatttccacc tcattccccc tttaagctga gaatggggtc
     3121 tgcctataga agcttccaga gacagctgag actgggtccc cggtgcctcc accctaggat
     3181 ctcaggcctg ccccaaactg cttcttacag cgtggaggtt tctctgattt ctcccttatc
     3241 tagcaacact tggttctttt ggctagtcag ggctgggatc ccagggggat ccacctgcag
     3301 gagtcagagg taaagaagca tccacacgga gggtagtggg gaaggaagcc cacatggcag
     3361 aagctctgca aacacagtcc gagggactga ggagtccata caatgcctca cacacaaagg
     3421 ccattgtcaa agcctcgggc ctggcagaaa gtgagggggg tggggccgct aggccacctc
     3481 ctgctcagcc acccctggtc ctcattctgg gatgagggga gggcctagga gaagccctcc
     3541 ctttcagtgc agattccctt cttgaatggg acattgagtt cctatcagcc accccagact
     3601 gggtcttccc atgtatataa tggaggaaga cccctcccag ccctctcagt cccccagctc
     3661 aaagtgcctc agttcctcca tctgctccca gatcccctgg cactctagac aggcatgccc
     3721 atgggaagga ccctgggatg gagaggcagg cttcgcggcc cttcatgcct gcccactggg
     3781 attccagtta actcacccca gggtgtcagg ggctctgcag gatggcaagt gttggggagg
     3841 agggaggcag accactctgt gccccacggg cactgaccca ccctgccata ggcttcctaa
     3901 gggagccccc caacccctcc cctcccctcc ctggtgctgc tattgaaccc cctgccagct
     3961 ccatttctgc cctggcaacc ctgggcccag caggtgggtc gtcgttgtct ggccctcact
     4021 gccctggccc cagcccaggc tgcccagtag ccacgtagca gaagtctgaa gccatgccag
     4081 ccctggtgcg gctctcccct ctacacattt ggggtactgg atagggtgaa gttaggggag
     4141 aaaattgtgg agtctttcca gccacagggc agagggaggt ggaggagctg ggctgctttt
     4201 cccagcagta ttagtgtccc ccaggacagg ggaccttttc cacattacat cactgcccca
     4261 tcccacctta caacactctg gcccctctgc ttggtcccct tttcccccag gcaggaggca
     4321 atcccagggg cctgcctgat agaggcattt cctgtccctg tctcctcctg catctccttt
     4381 atcctgcact gccaccctct attccccatt ctgtgttgga ctttgaaggc cccaagccca
     4441 gccaaagcac tgagttcccc cttaagacac ctccacaccc tccccacaag caaagcacaa
     4501 attttggggt ccatgtagca tgggccacgt aggaggctcc tgacttgcca ggggcccagc
     4561 ctcagcatac ccaccgagca gctgccagcc tgggctgagg gtgggcatga ggcaggagtc
     4621 agcacttgga cctagggatg tgaggttttc tgtgccccaa gtttgtggga aggtgggcac
     4681 tactgctggg cccacagaca cagccagctg gcaaaaggga ggtctagccc agcagagaga
     4741 tgaggacatt ttgcttctcc ttcatgccca cagcatgagc tgagcttctg ctttgctgga
     4801 aatgaaataa acttggtatg aattgtgcca aggcctcccc agttgtcatc ctgcctcttg
     4861 ttgccctccc tgtccttgcc ccccacccca cacccatgcc cctgtttcct tacagatttt
     4921 gatattgttc taatgtgtaa tagaaccagc cgagtcccct tttatcagaa gggtctgaaa
     4981 agcagcagca cagagtaggt gaacacaggc ctgcaagtgc gaccacctca gacccagtac
     5041 gtgtgcccac agtggacaca ctcacacctc caacacaccc acgcgcaggc atgtgtacac
     5101 gcatgtacac acgcatgcat gcacagccag atggccactc agcacagatg tggcagaggg
     5161 aatggtctga tcctgctgaa agccattaag gagaaacgaa tttcccagtg cccgggctgc
     5221 aagagagcct tataggggcc ctgtttcctg ggcatgcgct tcctctgcca gccaaccccc
     5281 acttgcccaa gtcactggtg caataacttt tctgccttcc tcagagcaga gaaattggga
     5341 attgtgttag gtgggtgtgg gcagctctgc tgagccaagc agacacggat gtcccctctt
     5401 ctgggaggag ggtagtgctc ccaggcctca ggagtccaga cagagacccc caaagcctga
     5461 ctgccaacag aaaccctctc ctagtgaggg gcaggtgggt gtgccccagg tccccacacc
     5521 cacagggagg cttcacacac tgcccagtac cggggatgcc aggaggcagg cccctctgct
     5581 gctgccactg ctgccaacac tgcccagctt gtgaggccag gaggagcccc tgtcccactc
     5641 ggtgctgctg ctcttctgac ccctgctgtg aggaatggga ttcttggtcg aaaaaattgg
     5701 ttttcctttt ttgtataaat gaaaagaatc caggagaagc tgccaccctc ccctcccagc
     5761 gtgatgcgct accttgcttc ggcgtcttgt cgccctttcc gcctttggtc cagggacagc
     5821 ccagcagatc ctcctggttc tgacctgggg ggtgtttgca tcaccccctt ttacttgtat
     5881 taaaaaaaaa tgatgggttg aaaatgtact gaggattaaa aatgtacttt tttataaata
     5941 aagtgtttaa aacaaa
//
