LOCUS       NM_018090               2018 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NECAP endocytosis associated 2 (NECAP2), transcript
            variant 1, mRNA.
ACCESSION   NM_018090
VERSION     NM_018090.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2018)
  AUTHORS   Chamberland,J.P., Antonow,L.T., Dias Santos,M. and Ritter,B.
  TITLE     NECAP2 controls clathrin coat recruitment to early endosomes for
            fast endocytic recycling
  JOURNAL   J Cell Sci 129 (13), 2625-2637 (2016)
   PUBMED   27206861
  REMARK    GeneRIF: NECAP2 does not regulate the clathrin-mediated endocytosis
            of these cargos, the degradation of EGFR or the recycling of
            transferrin along the slow, Rab11-dependent recycling pathway. We
            show that protein knockdown of NECAP2 leads to enlarged early
            endosomes and causes the loss of the clathrin adapter AP-1 from the
            organelle.
REFERENCE   4  (bases 1 to 2018)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   5  (bases 1 to 2018)
  AUTHORS   Ritter,B., Philie,J., Girard,M., Tung,E.C., Blondeau,F. and
            McPherson,P.S.
  TITLE     Identification of a family of endocytic proteins that define a new
            alpha-adaptin ear-binding motif
  JOURNAL   EMBO Rep 4 (11), 1089-1095 (2003)
   PUBMED   14555962
  REMARK    GeneRIF: Describes identification and characterization of rat
            NECAP1 and NECAP2 and their roles in endocytosis.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM751287.1, BC018914.2 and
            BQ005870.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_018090.4.
            
            Summary: This gene likely encodes a member of the
            adaptin-ear-binding coat-associated protein family. Studies of a
            similar protein in rat suggest a role in clathrin-mediated
            endocytosis. Alternatively spliced transcript variants have been
            described. [provided by RefSeq, Feb 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC018914.2, SRR3476690.1119785.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337132.10/ ENSP00000338746.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BM751287.1         53-66
            15-1531             BC018914.2         1-1517
            1532-1532           BQ005870.1         507-507             c
            1533-2018           BC018914.2         1519-2004
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..2018
                     /gene="NECAP2"
                     /note="NECAP endocytosis associated 2"
                     /db_xref="GeneID:55707"
                     /db_xref="HGNC:HGNC:25528"
                     /db_xref="MIM:611624"
     exon            1..130
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..830
                     /gene="NECAP2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     adaptin-ear-binding coat-associated protein 2"
                     /codon_start=1
                     /product="adaptin ear-binding coat-associated protein 2
                     isoform 1"
                     /protein_id="NP_060560.1"
                     /db_xref="CCDS:CCDS173.1"
                     /db_xref="GeneID:55707"
                     /db_xref="HGNC:HGNC:25528"
                     /db_xref="MIM:611624"
                     /translation="MEESGYESVLCVKPDVHVYRIPPRATNRGYRAAEWQLDQPSWSG
                     RLRITAKGQMAYIKLEDRTSGELFAQAPVDQFPGTAVESVTDSSRYFVIRIEDGNGRR
                     AFIGIGFGDRGDAFDFNVALQDHFKWVKQQCEFAKQAQNPDQGPKLDLGFKEGQTIKL
                     NIANMKKKEGAAGNPRVRPASTGGLSLLPPPPGGKTSTLIPPPGEQLAVGGSLVQPAV
                     APSSGGAPVPWPQPNPATADIWGDFTKSTGSTSSQTQPGTGWVQF"
     exon            131..231
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            232..336
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            337..418
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            419..527
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            528..705
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            706..781
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..2018
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1996..2001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECAP2"
                     /note="hexamer: AATAAA"
     polyA_site      2018
                     /gene="NECAP2"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtcgcc ggaagttcgg tgggctccag gcgtcgcgat ggaggagagc gggtacgagt
       61 cggtgctctg tgtcaagcct gacgtccacg tctaccgcat ccctccgcgg gctaccaacc
      121 gtggctacag ggctgcggag tggcagctgg accagccatc atggagtggc cggctgagga
      181 tcactgcaaa gggacagatg gcctacatca agctggagga caggacgtca ggggagctct
      241 ttgctcaggc cccggtggat cagtttcctg gcacagctgt ggagagtgtg acggattcca
      301 gcaggtactt cgtgatccgc atcgaagatg gaaatgggcg acgggcgttt attggaattg
      361 gcttcgggga ccgaggtgat gcctttgact tcaatgttgc attgcaggac catttcaagt
      421 gggtgaaaca gcagtgtgaa tttgcaaaac aagcccagaa cccagaccaa ggccctaaac
      481 tggacctggg cttcaaggag ggccagacca tcaagctcaa catcgcaaac atgaagaaga
      541 aggaaggagc agctgggaat ccccgagtcc ggcctgccag cacaggaggg ctgagcctgc
      601 ttccccctcc cccagggggg aaaacctcca ccctgatccc tccccctggg gagcagttgg
      661 ctgtgggggg atccctcgtc cagccagcag ttgctcccag ttcaggaggt gctcctgtac
      721 cctggccaca gcccaatcct gccactgctg acatctgggg agactttacc aaatctacag
      781 gatcaacttc cagccagacc cagccaggca caggctgggt ccagttctga cctgagcacg
      841 gtttttcctc atgtgacttc tgggaaggcg ctccctcatc tgggccaaag gaaggaggac
      901 gaagccctcc tcagctggcc tgtgtttggg gcatgaatct ctcctctcct ccttgtctgg
      961 ctctgttgac aaaccgggca tgtttggcag taaattggca ccgtgtcaca ctgtttcctg
     1021 ggattcaagt atgcaaccag aacacaggag aagaaaagct ccaggatccc tgtccccatc
     1081 tgtcctcttg atgtgagaga gactctgaga cttcttccat cgcaatgacc tgtattaaac
     1141 acaagccccc caagcaaaag aagaggttga gtttgctgcc aggattcaga tcagcccttc
     1201 ccagggtctg caggtgtcac atgatcacag ttcagcggga ggctttccgt acccacactg
     1261 gctgtagcca cttcagtcca tctgccctcc agaggagggg tttcttcctg atttttagca
     1321 ggtttagagg ctgcagcttg agctacaatc aggagggaaa ttggaaggat tagcagcttt
     1381 taaaaatgtt taaatatttt gctttgctaa tgtgctgatc cgcactaact catctttgca
     1441 aaaggaactg ctccctcggc gtgccccagc tggggcctct gaagggattc ctcactgtgg
     1501 gcagctgccc tgagcttcag gcagcagtgt ttatctctgg ccagttgtct ggtttccatg
     1561 tattctaggc caggtaggca acacagagcc aaggcgggtg ctggaagcca gacggaacag
     1621 tgttggggca ggaaggtgga tgctgttgtc atggagctgt gggagttggc actctgtctg
     1681 ctggtggccc tctcggctca catgttcaca gtgcagctcc tggcagactt gggttttctc
     1741 tttggtggtt tctaaagtgc cttatctgca aacaacttct tttctccttc aggaactgtg
     1801 aatggctaga agaaggagct cagtaaacta gaagtccagg gttgcttggt ttactggttt
     1861 ataagaaatc tgaaagcacc tctgacattc cttttattaa ctcacctctc agttgaaaga
     1921 tttcttcttt gaaaggtcaa gaccgtgaac tgaaaaaagt gttggccttt ttgcgggacc
     1981 agatttttaa gataaaataa atatttttac ttctgtca
//
