LOCUS       NM_001145278            1940 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens NECAP endocytosis associated 2 (NECAP2), transcript
            variant 3, mRNA.
ACCESSION   NM_001145278
VERSION     NM_001145278.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1940)
  AUTHORS   Chamberland,J.P., Antonow,L.T., Dias Santos,M. and Ritter,B.
  TITLE     NECAP2 controls clathrin coat recruitment to early endosomes for
            fast endocytic recycling
  JOURNAL   J Cell Sci 129 (13), 2625-2637 (2016)
   PUBMED   27206861
  REMARK    GeneRIF: NECAP2 does not regulate the clathrin-mediated endocytosis
            of these cargos, the degradation of EGFR or the recycling of
            transferrin along the slow, Rab11-dependent recycling pathway. We
            show that protein knockdown of NECAP2 leads to enlarged early
            endosomes and causes the loss of the clathrin adapter AP-1 from the
            organelle.
REFERENCE   4  (bases 1 to 1940)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   5  (bases 1 to 1940)
  AUTHORS   Ritter,B., Philie,J., Girard,M., Tung,E.C., Blondeau,F. and
            McPherson,P.S.
  TITLE     Identification of a family of endocytic proteins that define a new
            alpha-adaptin ear-binding motif
  JOURNAL   EMBO Rep 4 (11), 1089-1095 (2003)
   PUBMED   14555962
  REMARK    GeneRIF: Describes identification and characterization of rat
            NECAP1 and NECAP2 and their roles in endocytosis.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM751287.1, AK302227.1,
            BC018914.2 and BQ005870.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001145278.1.
            
            Summary: This gene likely encodes a member of the
            adaptin-ear-binding coat-associated protein family. Studies of a
            similar protein in rat suggest a role in clathrin-mediated
            endocytosis. Alternatively spliced transcript variants have been
            described. [provided by RefSeq, Feb 2009].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. This
            results in a shorter protein (isoform 3) compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.3017102.1,
                                           SRR14038195.1213425.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BM751287.1         53-54
            3-533               AK302227.1         1-531
            534-1453            BC018914.2         598-1517
            1454-1454           BQ005870.1         507-507             c
            1455-1940           BC018914.2         1519-2004
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..1940
                     /gene="NECAP2"
                     /note="NECAP endocytosis associated 2"
                     /db_xref="GeneID:55707"
                     /db_xref="HGNC:HGNC:25528"
                     /db_xref="MIM:611624"
     exon            1..52
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..752
                     /gene="NECAP2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     adaptin-ear-binding coat-associated protein 2"
                     /codon_start=1
                     /product="adaptin ear-binding coat-associated protein 2
                     isoform 3"
                     /protein_id="NP_001138750.1"
                     /db_xref="CCDS:CCDS44067.1"
                     /db_xref="GeneID:55707"
                     /db_xref="HGNC:HGNC:25528"
                     /db_xref="MIM:611624"
                     /translation="MEESGAAEWQLDQPSWSGRLRITAKGQMAYIKLEDRTSGELFAQ
                     APVDQFPGTAVESVTDSSRYFVIRIEDGNGRRAFIGIGFGDRGDAFDFNVALQDHFKW
                     VKQQCEFAKQAQNPDQGPKLDLGFKEGQTIKLNIANMKKKEGAAGNPRVRPASTGGLS
                     LLPPPPGGKTSTLIPPPGEQLAVGGSLVQPAVAPSSGGAPVPWPQPNPATADIWGDFT
                     KSTGSTSSQTQPGTGWVQF"
     exon            53..153
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            154..258
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            259..340
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            341..449
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            450..627
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            628..703
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..1940
                     /gene="NECAP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1918..1923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECAP2"
                     /note="hexamer: AATAAA"
     polyA_site      1940
                     /gene="NECAP2"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtcgcc ggaagttcgg tgggctccag gcgtcgcgat ggaggagagc ggggctgcgg
       61 agtggcagct ggaccagcca tcatggagtg gccggctgag gatcactgca aagggacaga
      121 tggcctacat caagctggag gacaggacgt caggggagct ctttgctcag gccccggtgg
      181 atcagtttcc tggcacagct gtggagagtg tgacggattc cagcaggtac ttcgtgatcc
      241 gcatcgaaga tggaaatggg cgacgggcgt ttattggaat tggcttcggg gaccgaggtg
      301 atgcctttga cttcaatgtt gcattgcagg accatttcaa gtgggtgaaa cagcagtgtg
      361 aatttgcaaa acaagcccag aacccagacc aaggccctaa actggacctg ggcttcaagg
      421 agggccagac catcaagctc aacatcgcaa acatgaagaa gaaggaagga gcagctggga
      481 atccccgagt ccggcctgcc agcacaggag ggctgagcct gcttccccct cccccagggg
      541 ggaaaacctc caccctgatc cctccccctg gggagcagtt ggctgtgggg ggatccctcg
      601 tccagccagc agttgctccc agttcaggag gtgctcctgt accctggcca cagcccaatc
      661 ctgccactgc tgacatctgg ggagacttta ccaaatctac aggatcaact tccagccaga
      721 cccagccagg cacaggctgg gtccagttct gacctgagca cggtttttcc tcatgtgact
      781 tctgggaagg cgctccctca tctgggccaa aggaaggagg acgaagccct cctcagctgg
      841 cctgtgtttg gggcatgaat ctctcctctc ctccttgtct ggctctgttg acaaaccggg
      901 catgtttggc agtaaattgg caccgtgtca cactgtttcc tgggattcaa gtatgcaacc
      961 agaacacagg agaagaaaag ctccaggatc cctgtcccca tctgtcctct tgatgtgaga
     1021 gagactctga gacttcttcc atcgcaatga cctgtattaa acacaagccc cccaagcaaa
     1081 agaagaggtt gagtttgctg ccaggattca gatcagccct tcccagggtc tgcaggtgtc
     1141 acatgatcac agttcagcgg gaggctttcc gtacccacac tggctgtagc cacttcagtc
     1201 catctgccct ccagaggagg ggtttcttcc tgatttttag caggtttaga ggctgcagct
     1261 tgagctacaa tcaggaggga aattggaagg attagcagct tttaaaaatg tttaaatatt
     1321 ttgctttgct aatgtgctga tccgcactaa ctcatctttg caaaaggaac tgctccctcg
     1381 gcgtgcccca gctggggcct ctgaagggat tcctcactgt gggcagctgc cctgagcttc
     1441 aggcagcagt gtttatctct ggccagttgt ctggtttcca tgtattctag gccaggtagg
     1501 caacacagag ccaaggcggg tgctggaagc cagacggaac agtgttgggg caggaaggtg
     1561 gatgctgttg tcatggagct gtgggagttg gcactctgtc tgctggtggc cctctcggct
     1621 cacatgttca cagtgcagct cctggcagac ttgggttttc tctttggtgg tttctaaagt
     1681 gccttatctg caaacaactt cttttctcct tcaggaactg tgaatggcta gaagaaggag
     1741 ctcagtaaac tagaagtcca gggttgcttg gtttactggt ttataagaaa tctgaaagca
     1801 cctctgacat tccttttatt aactcacctc tcagttgaaa gatttcttct ttgaaaggtc
     1861 aagaccgtga actgaaaaaa gtgttggcct ttttgcggga ccagattttt aagataaaat
     1921 aaatattttt acttctgtca
//
