LOCUS       NM_031232               2009 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens N-terminal EF-hand calcium binding protein 3 (NECAB3),
            transcript variant 2, mRNA.
ACCESSION   NM_031232
VERSION     NM_031232.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2009)
  AUTHORS   Li,Z., Feng,C., Guo,J., Hu,X. and Xie,D.
  TITLE     GNAS-AS1/miR-4319/NECAB3 axis promotes migration and invasion of
            non-small cell lung cancer cells by altering macrophage
            polarization
  JOURNAL   Funct Integr Genomics 20 (1), 17-28 (2020)
   PUBMED   31267263
  REMARK    GeneRIF: GNAS-AS1/miR-4319/NECAB3 axis promotes tumor progression
            of non-small cell lung cancer by altering macrophage polarization.
REFERENCE   4  (bases 1 to 2009)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 2009)
  AUTHORS   Nakaoka,H.J., Hara,T., Yoshino,S., Kanamori,A., Matsui,Y.,
            Shimamura,T., Sato,H., Murakami,Y., Seiki,M. and Sakamoto,T.
  TITLE     NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during
            Normoxia and Enhances Tumourigenicity of Cancer Cells
  JOURNAL   Sci Rep 6, 22784 (2016)
   PUBMED   26948053
  REMARK    GeneRIF: Results suggest that NECAB3, a novel Mint3-binding
            protein, activates HIF-1 to promote normoxic glycolysis and
            tumorigenicity by forming a ternary complex with Mint3 and FIH-1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2009)
  AUTHORS   Sumioka,A., Imoto,S., Martins,R.N., Kirino,Y. and Suzuki,T.
  TITLE     XB51 isoforms mediate Alzheimer's beta-amyloid peptide production
            by X11L (X11-like protein)-dependent and -independent mechanisms
  JOURNAL   Biochem J 374 (Pt 1), 261-268 (2003)
   PUBMED   12780348
  REMARK    GeneRIF: hXB51 isoforms regulate Abeta generation differently,
            either enhancing it by modifying the association of X11L with APP
            or suppressing it in an X11L-independent manner
REFERENCE   7  (bases 1 to 2009)
  AUTHORS   Sugita,S., Ho,A. and Sudhof,T.C.
  TITLE     NECABs: a family of neuronal Ca(2+)-binding proteins with an
            unusual domain structure and a restricted expression pattern
  JOURNAL   Neuroscience 112 (1), 51-63 (2002)
   PUBMED   12044471
REFERENCE   8  (bases 1 to 2009)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (bases 1 to 2009)
  AUTHORS   Lee,D.S., Tomita,S., Kirino,Y. and Suzuki,T.
  TITLE     Regulation of X11L-dependent amyloid precursor protein metabolism
            by XB51, a novel X11L-binding protein
  JOURNAL   J Biol Chem 275 (30), 23134-23138 (2000)
   PUBMED   10833507
REFERENCE   10 (bases 1 to 2009)
  AUTHORS   Sugita,S. and Sudhof,T.C.
  TITLE     Specificity of Ca2+-dependent protein interactions mediated by the
            C2A domains of synaptotagmins
  JOURNAL   Biochemistry 39 (11), 2940-2949 (2000)
   PUBMED   10715114
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121906.18.
            
            On Jun 2, 2019 this sequence version replaced NM_031232.3.
            
            Summary: The protein encoded by this gene interacts with the
            amino-terminal domain of the neuron-specific X11-like protein
            (X11L), inhibits the association of X11L with amyloid precursor
            protein through a non-competitive mechanism, and abolishes the
            suppression of beta-amyloid production by X11L. This protein,
            together with X11L, may play an important role in the regulatory
            system of amyloid precursor protein metabolism and beta-amyloid
            generation. The protein is phosphorylated by NIMA-related expressed
            kinase 2, and localizes to the Golgi apparatus. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2), also known as XB51alpha,
            represents the longer transcript and encodes the longer isoform
            (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.1393625.1,
                                           SRR14038194.3007249.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000246190.11/ ENSP00000246190.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-205               AL121906.18        50661-50865         c
            206-230             AL121906.18        48835-48859         c
            231-339             AL121906.18        47121-47229         c
            340-365             AL121906.18        46124-46149         c
            366-463             AL121906.18        45812-45909         c
            464-600             AL121906.18        36696-36832         c
            601-719             AL121906.18        36322-36440         c
            720-955             AL121906.18        35934-36169         c
            956-1068            AL121906.18        35159-35271         c
            1069-1146           AL121906.18        34914-34991         c
            1147-1238           AL121906.18        34379-34470         c
            1239-2009           AL121906.18        33524-34294         c
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.22"
     gene            1..2009
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /note="N-terminal EF-hand calcium binding protein 3"
                     /db_xref="GeneID:63941"
                     /db_xref="HGNC:HGNC:15851"
                     /db_xref="MIM:612478"
     exon            1..205
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1267
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /note="isoform 2 is encoded by transcript variant 2;
                     synaptotagmin interacting protein STIP3; synaptotagmin
                     interacting protein 2; neuronal calcium-binding protein
                     NECAB3; EF-hand calcium binding protein 3; amyloid beta
                     (A4) precursor protein-binding, family A, member 2 binding
                     protein; Nek2-interacting protein 1; X11L-binding protein
                     51; neuronal calcium-binding protein 3; amyloid beta A4
                     protein-binding family A member 2-binding protein"
                     /codon_start=1
                     /product="N-terminal EF-hand calcium-binding protein 3
                     isoform 2"
                     /protein_id="NP_112509.3"
                     /db_xref="CCDS:CCDS42866.1"
                     /db_xref="GeneID:63941"
                     /db_xref="HGNC:HGNC:15851"
                     /db_xref="MIM:612478"
                     /translation="MACAGLLTVCLLRPPAPQPQPQTPRHPQLAPDPGPAGHTLFQDV
                     FRRADKNDDGKLSFEEFQNYFADGVLSLGELQELFSGIDGHLTDNLETEKLCDYFSEH
                     LGVYRPVLAALESLNRAVLAAMDATKLEYERASKVDQFVTRFLLRETVSQLQALQSSL
                     EGASDTLEAQAHGWRSDAESVEAQSRLCGSRRAGRRALRSVSRSSTWSPGSSDTGRSS
                     EAEMQWRLQVNRLQELIDQLECKVRAVGPGPHKGGPSWYPPEPGPCWRPGPHSVPSQA
                     PRLEPLREEDLAKGPDLHILMAQRQVQVAEEGLQDFHRALRCYVDFTGAQSHCLHVSA
                     QKMLDGASFTLYEFWQDEASWRRHQQSPGSKAFQRILIDHLRAPDTLTTVFFPASWWI
                     MNNN"
     exon            206..230
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            231..339
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            340..365
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            366..463
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            464..600
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            601..719
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            720..955
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1068
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1146
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1238
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..2009
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1987..1992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /note="hexamer: AATAAA"
     polyA_site      2009
                     /gene="NECAB3"
                     /gene_synonym="APBA2BP; dJ63M2.4; dJ63M2.5; EFCBP3; NIP1;
                     STIP3; SYTIP2; XB51"
                     /note="major polyA site"
ORIGIN      
        1 ctctagccac accgagtccg ccgcggcgtc cagggtcggc agcaaccgca gccgagcccg
       61 agcgggtggc ggcgccatgg cgtgcgcggg gctgctcacc gtgtgcctgc tccggccgcc
      121 cgcgccccag ccccagcccc agaccccgcg gcacccccag ctcgcgcccg acccggggcc
      181 cgccggacac acgctcttcc aggacgtttt ccgcagagca gacaagaatg atgatgggaa
      241 gctctcattt gaggaattcc agaattactt tgccgatggg gttctcagcc tgggggagct
      301 gcaggaactg ttcagcggca ttgatgggca tctcaccgac aatttagaaa cagaaaaact
      361 gtgtgactac ttctcagagc acctgggtgt ctaccggccg gtgctggctg cattggaatc
      421 gctgaaccgt gcagtgctcg ctgccatgga tgccaccaag ctggagtacg agagggcctc
      481 caaagtggac cagtttgtga cgcgcttcct gctgcgggag acggtgagcc agctgcaagc
      541 ccttcagagc tcgctggagg gggcgtcaga taccctggag gcccaggccc atggctggcg
      601 gtcagatgca gagagcgtgg aggcgcagag caggctctgc ggcagccggc gggcaggacg
      661 ccgagccctg aggagtgtca gccggtcatc cacctggtcc cccggctctt ctgacacagg
      721 gcgcagctca gaggccgaga tgcagtggcg gctccaggtg aaccgcctcc aggagctcat
      781 cgaccagctc gagtgcaagg tgagggccgt ggggccaggg ccccacaagg gaggaccctc
      841 ctggtatcca ccagagccag gcccatgctg gaggcccggc ccacactctg tgccctcaca
      901 ggccccccgg ctggaacccc tgcgtgaaga ggacctggcc aaggggcctg acttgcacat
      961 cctcatggcc cagaggcagg tccaggtggc agaggaaggc ctgcaggact tccaccgagc
     1021 cctgcgctgc tatgtggact tcacaggggc ccagagccat tgtctgcatg tgtccgccca
     1081 gaagatgctg gacggtgcct ccttcaccct gtatgagttc tggcaggatg aggcctcctg
     1141 gagaaggcac cagcagtcgc ctggcagcaa ggccttccag cgcatcctca tcgaccacct
     1201 gcgggccccg gacaccctca ccactgtgtt cttcccagcc tcctggtgga taatgaataa
     1261 caactgagcc agacctgcac acgccgaggg ccccgggacc ctgcctgcct ccctctggag
     1321 ccttctggac tggccagccc agcgcaaaga ccaggacttg tctcctcctg gacttgggcc
     1381 tggtggaagg gctctcagcc cagggatcag ggactgggct gcttgctttc tatttattat
     1441 tgatttattt atccctgtat tttattgttt gtcacttcag cctccaagct actttggctt
     1501 ctgcctggct caggagacct ggattctggt ccctgctgtc cctgcctggc tgagcgaccc
     1561 agggcaggtc ccgtccctgt ctgggtgggc ctcaggccat ctggccgcct agcgagggtc
     1621 ctgctttctt cctgctgtct tgggaccagg cttctgctcc tcctcagcca gcggcagaag
     1681 cagggctgat gctcagggac cccccaaccc tcaaaccttg cctccgaggc tgggcctgag
     1741 ctgggtgccc cgtcgccctc ccaggccact aggctctgac tcttccagca cccatggatg
     1801 tgccagaggc ctctgggttg gtttgcatgt catttgcata tcgtttgcat gccctcatcc
     1861 cacccattct cagggcagta tgggaagccc caaggtgacc tggccaagta gcaataattt
     1921 gggtccaggg tcacctattg cccccagagg cctctgaacc ccaggctggg accccagcac
     1981 agaggcaata aaggcagtgg tcccttcca
//
