LOCUS       NM_019065               1996 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2),
            transcript variant 1, mRNA.
ACCESSION   NM_019065
VERSION     NM_019065.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Bueno,D., Narayan Dey,P., Schacht,T., Wolf,C., Wullner,V.,
            Morpurgo,E., Rojas-Charry,L., Sessinghaus,L., Leukel,P., Sommer,C.,
            Radyushkin,K., Florin,L., Baumgart,J., Stamm,P., Daiber,A.,
            Horta,G., Nardi,L., Vasic,V., Schmeisser,M.J., Hellwig,A.,
            Oskamp,A., Bauer,A., Anand,R., Reichert,A.S., Ritz,S., Nocera,G.,
            Jacob,C., Peper,J., Silies,M., Frauenknecht,K.B.M., Schafer,M.K.E.
            and Methner,A.
  TITLE     NECAB2 is an endosomal protein important for striatal function
  JOURNAL   Free Radic Biol Med 208, 643-656 (2023)
   PUBMED   37722569
  REMARK    GeneRIF: NECAB2 is an endosomal protein important for striatal
            function.
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1996)
  AUTHORS   Zhang,M.D., Barde,S., Szodorai,E., Josephson,A., Mitsios,N.,
            Watanabe,M., Attems,J., Lubec,G., Kovacs,G.G., Uhlen,M., Mulder,J.,
            Harkany,T. and Hokfelt,T.
  TITLE     Comparative anatomical distribution of neuronal calcium-binding
            protein (NECAB) 1 and -2 in rodent and human spinal cord
  JOURNAL   Brain Struct Funct 221 (7), 3803-3823 (2016)
   PUBMED   26843217
  REMARK    GeneRIF: Study suggests that the phenotypic segregation of Neuronal
            calcium-binding protein 1 and -2 (NECAB1 and -2) to respective
            excitatory and inhibitory spinal systems can underpin functional
            modalities in determining the fidelity of synaptic
            neurotransmission and neuronal responsiveness, and might bear
            translational relevance to humans.
            Erratum:[Brain Struct Funct. 2016 Sep;221(7):3843. doi:
            10.1007/s00429-016-1236-7.. PMID: 27206425]
REFERENCE   4  (bases 1 to 1996)
  AUTHORS   Yachie,N., Petsalaki,E., Mellor,J.C., Weile,J., Jacob,Y., Verby,M.,
            Ozturk,S.B., Li,S., Cote,A.G., Mosca,R., Knapp,J.J., Ko,M., Yu,A.,
            Gebbia,M., Sahni,N., Yi,S., Tyagi,T., Sheykhkarimli,D., Roth,J.F.,
            Wong,C., Musa,L., Snider,J., Liu,Y.C., Yu,H., Braun,P.,
            Stagljar,I., Hao,T., Calderwood,M.A., Pelletier,L., Aloy,P.,
            Hill,D.E., Vidal,M. and Roth,F.P.
  TITLE     Pooled-matrix protein interaction screens using Barcode Fusion
            Genetics
  JOURNAL   Mol Syst Biol 12 (4), 863 (2016)
   PUBMED   27107012
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1996)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   6  (bases 1 to 1996)
  AUTHORS   Canela,L., Fernandez-Duenas,V., Albergaria,C., Watanabe,M.,
            Lluis,C., Mallol,J., Canela,E.I., Franco,R., Lujan,R. and
            Ciruela,F.
  TITLE     The association of metabotropic glutamate receptor type 5 with the
            neuronal Ca2+-binding protein 2 modulates receptor function
  JOURNAL   J Neurochem 111 (2), 555-567 (2009)
   PUBMED   19694902
  REMARK    GeneRIF: NECAB2 by its physical interaction with mGlu(5b) receptor
            modulates receptor function
REFERENCE   7  (bases 1 to 1996)
  AUTHORS   Canela,L., Lujan,R., Lluis,C., Burgueno,J., Mallol,J., Canela,E.I.,
            Franco,R. and Ciruela,F.
  TITLE     The neuronal Ca(2+) -binding protein 2 (NECAB2) interacts with the
            adenosine A(2A) receptor and modulates the cell surface expression
            and function of the receptor
  JOURNAL   Mol Cell Neurosci 36 (1), 1-12 (2007)
   PUBMED   17689978
REFERENCE   8  (bases 1 to 1996)
  AUTHORS   Sugita,S., Ho,A. and Sudhof,T.C.
  TITLE     NECABs: a family of neuronal Ca(2+)-binding proteins with an
            unusual domain structure and a restricted expression pattern
  JOURNAL   Neuroscience 112 (1), 51-63 (2002)
   PUBMED   12044471
REFERENCE   9  (bases 1 to 1996)
  AUTHORS   Bernier,G., Vukovich,W., Neidhardt,L., Herrmann,B.G. and Gruss,P.
  TITLE     Isolation and characterization of a downstream target of Pax6 in
            the mammalian retinal primordium
  JOURNAL   Development 128 (20), 3987-3994 (2001)
   PUBMED   11641222
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC040169.7.
            
            On Jul 1, 2020 this sequence version replaced NM_019065.2.
            
            Summary: The protein encoded by this gene is a neuronal
            calcium-binding protein that binds to and modulates the function of
            at least two receptors, adenosine A(2A) receptor and metabotropic
            glutamate receptor type 5. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY299331.1, AK127376.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305202.9/ ENSP00000307449.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-606               AC040169.7         24770-25375
            607-631             AC040169.7         28677-28701
            632-740             AC040169.7         34970-35078
            741-766             AC040169.7         37365-37390
            767-864             AC040169.7         37556-37653
            865-1001            AC040169.7         47020-47156
            1002-1120           AC040169.7         50828-50946
            1121-1200           AC040169.7         51135-51214
            1201-1254           AC040169.7         53742-53795
            1255-1367           AC040169.7         54731-54843
            1368-1445           AC040169.7         57250-57327
            1446-1537           AC040169.7         58351-58442
            1538-1996           AC040169.7         58844-59302
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q23.3"
     gene            1..1996
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /note="N-terminal EF-hand calcium binding protein 2"
                     /db_xref="GeneID:54550"
                     /db_xref="HGNC:HGNC:23746"
                     /db_xref="MIM:618130"
     exon            1..606
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             406..1566
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neuronal calcium binding 2; EF-hand calcium-binding
                     protein 2; neuronal calcium-binding protein 2;
                     synaptotagmin-interacting protein 2"
                     /codon_start=1
                     /product="N-terminal EF-hand calcium-binding protein 2
                     isoform 1"
                     /protein_id="NP_061938.2"
                     /db_xref="CCDS:CCDS10940.1"
                     /db_xref="GeneID:54550"
                     /db_xref="HGNC:HGNC:23746"
                     /db_xref="MIM:618130"
                     /translation="MCERAARLCRAGAHRLLREPPQQGRALGGLLRWVGARMGEPRES
                     LAPAAPADPGPASPRGGTAVILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEDL
                     FHTIDSDNTNHVDTKELCDYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSN
                     VDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPT
                     PASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLS
                     DEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFV
                     IYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD"
     exon            607..631
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            632..740
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            741..766
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            767..864
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            865..1001
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1120
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1200
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1254
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1367
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1445
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1537
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1996
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1976..1981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /note="hexamer: AATAAA"
     polyA_site      1996
                     /gene="NECAB2"
                     /gene_synonym="EFCBP2; stip-2"
                     /note="major polyA site"
ORIGIN      
        1 ctctgctgcg cgccgcgagt gggagggggg actttagaag cggggagagc aggagacttt
       61 tggggagcag gccccagggg cgccgccccg cccccaggcc ccacataccc ctcccctccg
      121 ggtagccccc tctgtggctg cgcccgcgcc cctcgccccg gcgggcagtc ctcaggcccc
      181 gcgcccggcc ggcgggggtg ggggcggcgg ggagcgggca cgtggctcgg ggtccggccg
      241 gcccgccccc cggcgccggc cagtccccgc ggtgtccgcg gccgcggggg cagcgggaga
      301 gagggcgggg cggcgcgggc agcgcgggga gggggtcgcg cgggggcggg ccgcagctgg
      361 gcgggggtcg gcgggcttcc gggcggcggc ggcgggcgcg gcgcgatgtg cgagcgggcg
      421 gcgcgcctgt gcagggccgg cgcgcacagg ctgctccggg agccgccgca gcagggccgg
      481 gcgctgggcg ggctgctgcg ctgggtgggc gccaggatgg gcgagccccg ggagtcgctg
      541 gcccccgccg cccccgcgga ccccggccca gcctcgccgc gcgggggcac cgccgtcatc
      601 ctggacattt tccgccgtgc ggacaaaaat gatgatggga agctgtcctt ggaggaattc
      661 cagctcttct ttgcagatgg cgtccttaat gagaaagaac tggaggatct ctttcacacg
      721 attgactctg acaacaccaa ccatgtggac accaaggagc tgtgtgatta ctttgtggac
      781 cacatgggtg actatgagga tgtcctggcc tccctggaga ccttgaatca ctctgtcctg
      841 aaggccatgg gttataccaa gaaggtatat gagggtggga gcaacgtgga ccagtttgtg
      901 acccgcttcc tcctgaagga gacggccaat cagatccagt cgctgctgag ctcagtggag
      961 agtgcggtgg aggccatcga ggaacagacc agccagctcc gacagaacca catcaaaccc
     1021 agccacagcg cggcacagac ctggtgtgga agccccactc ccgcctctgc ccccaaccac
     1081 aagctcatgg ctatggaaca aggcaagacc cttccatctg ccacggagga tgcaaaggaa
     1141 gagggtctgg aagcccagat cagccgcttg gcagagctga ttgggaggct ggagagcaaa
     1201 gcactgtggt tcgacctgca gcagcgcctg tcagatgaag atggcaccaa catgcacctg
     1261 cagctggtcc ggcaggagat ggccgtgtgc cccgagcaac tgagcgagtt tctggactct
     1321 ctgcgccagt atctgcgggg gaccactggc gtgaggaact gcttccacat cactgccgtg
     1381 aggctctcag atggcttcac ctttgtcatc tatgagttct gggagacaga ggaggcgtgg
     1441 aagaggcacc tgcagagccc cctgtgtaag gcgttccggc acgtcaaggt ggacacactg
     1501 agccagcctg aggccctctc caggatcttg gtgccagctg cttggtgcac ggtgggacgg
     1561 gactgacagc ctcccagagg cccgtggagg agcccaccag ccccttcttc ttgtgaagga
     1621 aatcccgttt ttttctagac agacactttg gtgcagaagc ttcttttcaa tccatcctcc
     1681 acaagaaggt gtttccctgt tgttaagtga aggaggccgc ccctgccccc acctgagaag
     1741 gcagagcagt gtctgtgctg ccaggtcctg gtgaagccca aggttgaagg gggcggcttc
     1801 ctggagccag cacccctgcc tcctggtcct ggcctctccc ctacccctca catggccacg
     1861 catgacccac actgaccaca ccctgccctc ttcggtgaca ttcttctacc tagtaggagt
     1921 catgcccctg tagtgcccaa cctagccagg tagccaccac tgtgcccagg cgccaaataa
     1981 accctggttg ggaaga
//
