LOCUS       NM_022351               5049 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens N-terminal EF-hand calcium binding protein 1 (NECAB1),
            mRNA.
ACCESSION   NM_022351
VERSION     NM_022351.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Zhang,M.D., Barde,S., Szodorai,E., Josephson,A., Mitsios,N.,
            Watanabe,M., Attems,J., Lubec,G., Kovacs,G.G., Uhlen,M., Mulder,J.,
            Harkany,T. and Hokfelt,T.
  TITLE     Comparative anatomical distribution of neuronal calcium-binding
            protein (NECAB) 1 and -2 in rodent and human spinal cord
  JOURNAL   Brain Struct Funct 221 (7), 3803-3823 (2016)
   PUBMED   26843217
  REMARK    GeneRIF: Study suggests that the phenotypic segregation of Neuronal
            calcium-binding protein 1 and -2 (NECAB1 and -2) to respective
            excitatory and inhibitory spinal systems can underpin functional
            modalities in determining the fidelity of synaptic
            neurotransmission and neuronal responsiveness, and might bear
            translational relevance to humans.
            Erratum:[Brain Struct Funct. 2016 Sep;221(7):3843. doi:
            10.1007/s00429-016-1236-7.. PMID: 27206425]
REFERENCE   3  (bases 1 to 5049)
  AUTHORS   Wu,H., Li,D., Shan,Y., Wan,B., Hexige,S., Guo,J., Wu,C. and Yu,L.
  TITLE     EFCBP1/NECAB1, a brain-specifically expressed gene with highest
            abundance in temporal lobe, encodes a protein containing EF-hand
            and antibiotic biosynthesis monooxygenase domains
  JOURNAL   DNA Seq 18 (1), 73-79 (2007)
   PUBMED   17364817
REFERENCE   4  (bases 1 to 5049)
  AUTHORS   Sugita,S., Ho,A. and Sudhof,T.C.
  TITLE     NECABs: a family of neuronal Ca(2+)-binding proteins with an
            unusual domain structure and a restricted expression pattern
  JOURNAL   Neuroscience 112 (1), 51-63 (2002)
   PUBMED   12044471
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF414126.2.
            
            On Nov 23, 2018 this sequence version replaced NM_022351.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.195227.1,
                                           SRR1660809.70887.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2142363
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000417640.7/ ENSP00000387380.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5049              AF414126.2         172-5220
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q21.3"
     gene            1..5049
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /note="N-terminal EF-hand calcium binding protein 1"
                     /db_xref="GeneID:64168"
                     /db_xref="HGNC:HGNC:20983"
     exon            1..211
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..1168
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /note="synaptotagmin interacting protein 1; neuronal
                     calcium binding protein; EF-hand calcium-binding protein
                     1; neuronal calcium-binding protein 1"
                     /codon_start=1
                     /product="N-terminal EF-hand calcium-binding protein 1"
                     /protein_id="NP_071746.1"
                     /db_xref="CCDS:CCDS47889.1"
                     /db_xref="GeneID:64168"
                     /db_xref="HGNC:HGNC:20983"
                     /translation="MEDSQETSPSSNNSSEELSSALHLSKGMSIFLDILRRADKNDDG
                     KLSFEEFKAYFADGVLSGEELHELFHTIDTHNTNNLDTEELCEYFSQHLGEYENVLAA
                     LEDLNLSILKAMGKTKKDYQEASNLEQFVTRFLLKETLNQLQSLQNSLECAMETTEEQ
                     TRQERQGPAKPEVLSIQWPGKRSSRRVQRHNSFSPNSPQFNVSGPGLLEEDNQWMTQI
                     NRLQKLIDRLEKKDLKLEPPEEEIIEGNTKSHIMLVQRQMSVIEEDLEEFQLALKHYV
                     ESASSQSGCLRISIQKLSNESRYMIYEFWENSSVWNSHLQTNYSKTFQRSNVDFLETP
                     ELTSTMLVPASWWILNN"
     exon            212..236
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            237..345
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            346..371
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..469
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            470..606
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            607..728
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            729..805
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            806..859
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..972
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1050
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1142
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..5049
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5029..5034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /note="hexamer: AATAAA"
     polyA_site      5049
                     /gene="NECAB1"
                     /gene_synonym="EFCBP1; STIP-1"
                     /note="major polyA site"
ORIGIN      
        1 ctcccggatc cagagcccgg cggcggcgaa gcagcagctg cggccgcgcc cttgccagag
       61 ccggtgcgtc cgcctagccc cgctccgcct gaggccgtca gggctcccga ggatggaaga
      121 ttcccaggag acatcgccgt cctccaacaa ctcctcggag gagctcagct ctgctctgca
      181 cctgtccaag ggcatgtcga tcttcctcga catactgagg agagcagaca aaaatgatga
      241 tggaaaatta tcctttgaag aattcaaagc atattttgca gatggtgttc tcagtggaga
      301 agaattacac gagcttttcc ataccattga tacacataat actaataatc ttgacacaga
      361 agagctatgt gaatattttt ctcagcactt gggcgagtat gagaatgtac tagcagcact
      421 tgaagacctg aatctttcca tcctgaaggc aatgggcaaa acaaagaaag actaccaaga
      481 agcctccaat ttggaacaat tcgtaactag atttttattg aaggaaaccc tgaatcagct
      541 gcagtctctc cagaattccc tggaatgtgc catggaaact actgaggagc aaacccgtca
      601 agaaaggcaa gggccagcca agccagaagt cctgtcgatt caatggcctg gaaaacgatc
      661 aagccgccga gtccagagac acaacagctt ctccccaaac agccctcagt ttaatgtcag
      721 cggtccaggc ttattagaag aagacaacca gtggatgacc cagataaata gactccagaa
      781 attaattgat agactggaaa agaaggatct caaactcgaa ccaccagaag aagaaattat
      841 tgaagggaat actaaatctc acatcatgct tgtgcagcgg cagatgtctg tgatagaaga
      901 ggacctggaa gaattccagc tcgctctgaa acactacgtg gagagtgctt cctcccaaag
      961 tggatgcttg cgtatttcta tacagaagct ttcaaatgaa tctcgctaca tgatctatga
     1021 gttctgggag aatagtagtg tatggaatag ccaccttcag acaaattata gcaagacatt
     1081 ccaaagaagt aatgtggatt tcttggaaac tccagaactc acatctacaa tgctagttcc
     1141 tgcttcgtgg tggatcctga acaactagat gttcctagac attttcttta tggttccaag
     1201 tgcaaaacag gtgttcttat ctaaaacgtc aattagaaaa ttatctgcgg ttgttaatct
     1261 actgtatatt tttgtttggt atatttacta agtgcactct ttcaaaactt attctataac
     1321 tttatcaatt catgtgaatt ttagctcaat tttcaaagtt cactaatatt ctcaatattt
     1381 aatgctaaat gctttgctac attgtaactc acctaaaacc ttttagtgac aaaatcctaa
     1441 tatgtggaaa aaagcatatg cataaaggaa taatattgtg aaaatgaatc tgttatgata
     1501 aagaaaaaat aaagtggaaa cttttagagt attacttcat agggcagatt ttgtaaactg
     1561 tcgtatactg taaagggtta aatcagcgtt ttgtgatttt taagtaactg tgagtgaagt
     1621 ttattcttca acaatgtcta ctccatcccc aacccaactc acagccctat gactactatc
     1681 tttgcattag ttaaaaagtt agtatatagg catcaaacaa ccttggctgt aacctataga
     1741 atctctatcc acgtatcagg ttatagactg gtttttcaaa agtgaacaat cctgtgataa
     1801 gttggagtac catttagtaa tacagcaaca ttgtgtcatt tattagcatc ataattcttt
     1861 gttatgtaag ttaaatatat caagaaagaa gagactgttt ggaaaaatgt ggttcaagtt
     1921 ttatgctata tagttttggt atgcgataca gacagctaac ttttcttatg aaaaatacat
     1981 atttgcatgt aaacaatgat ttcaaaatac ttgaaaaata aaattttaac ccaaatgaat
     2041 aactaagaaa tataaaacaa gcacaaaatc ttagggaagt cataaaatag tagtgaaagt
     2101 attagacaga agacatctgt tttcgaattt caacactaga atgactaaaa ctatctacct
     2161 atagaactat ctgtagatag tatactatct acactctgct caacaagctc agaaattaaa
     2221 tatttttaat aataaaaatc tgttctggtt ataaaccttg ctaatgaaaa tacaatacat
     2281 ataaaaatgt atagccatgt tattttctag tataaattcc tttgaaacta taagtctttg
     2341 aggaaaatta taaggtaaaa ttttcctgtt tttccccctt tgaaaaactc aggaaaaaag
     2401 gaagattgaa ctaataaaat tttatttctt aaatataaat ttgacctaaa atattttctc
     2461 aaactaattc atgaaacagc aacttttacc aatacctttg tatactctca gttctcattc
     2521 agtataaata aaattttaaa atcctttcat agttctatta gaaataagta gtaaattttg
     2581 atatattgta catacacacg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2641 tatttgtgtg cctctggtca actctaagga tgacagacac tgtgtaacaa cacctgggtc
     2701 aactctttta atttatatac aaagcaaaga acaacattaa tggagatgca caatgattat
     2761 tcaaacaagc tatatatatg tacaaaggca aacagacaca taacagtctc tgcagactga
     2821 ttgtatatag taagaaaaga tcaaaagact ttaaaaccta aatgactttt gacatacaaa
     2881 ctcttcttga gaatgtttgt tgtaaatggt ttcaaaaata caaattatag ccaatcaaaa
     2941 cattgctttg gttggtgcat ttaagtatcc aactcaaaaa gcatatcaaa tattttgggt
     3001 actaggcagt ttccaaagta gcatggtagt attacttgtt aaaagggttc tgttttcatt
     3061 aacagtacta agtggaaggg atctgcagat tccaaactgg aataagctct atcatattct
     3121 gaaacaagaa ttagaatgac ttgagaacgg gcaaataaca aagcaaacca atataattat
     3181 atggtcattc tgaccccagc tcttatacaa attatacatg tatttttgtg tatgtttgtg
     3241 agagttgtat gtatgtgaat gtgtgtgagt gtgtattcac atacacatat atactggaac
     3301 ctatagtaga aaaggaaact agtagggcca aaaaaaaaaa gaaaaagaaa aagaaaaaag
     3361 aaaaaaaaag aaaaaactgg gacctaagta taaatatctc atcctaaagt aaacaataag
     3421 tttatagtta acgaagattt ttttctattt aaaaccccat tttcctaaag aacaaagtag
     3481 taaaataaaa aaaaatttaa aaaattaaaa aataaaaaaa agaggtcact aaaagaccaa
     3541 gatgggaaac gtaacatgga atacaagctt gaatctggga aacaagctca aaaaacagtg
     3601 aaaaaaaaag tactggttta ggagttcaaa attcagtgaa acaaactttt tgccaataga
     3661 cctagggatc taagaaatag aattagggag agtttacact tacttaccta tatatcagca
     3721 gatttttctg gaagaaaccc tttttttttt tttttttaaa cagtaacatc cagagtgaca
     3781 aattgcagga gatttaatgt ataaaatttc taggaattaa aagcttaaac ccaaaaattg
     3841 ttcatccata tataagaaat tatcgaatta aaacttaatg tatgtcaatt attttcaaat
     3901 gtctaaattc ctttggaaat aaaaatatca gttttacttt gaaattgctc aacttctgct
     3961 attcatatgg tctgattagt gacataagta gcagccgttt ttcaacttca gtttcattca
     4021 ctaccattgt ttccaaatta tcaatctctg ctagtaatta tgaatttaag accatattat
     4081 tatcaaaaac ctagagacca atcatatatc tgaaaagaaa tacccattaa aaattctgcc
     4141 tcctgtttat tgagaactat gcattgaaca ttctgaatca ttctaagcct ctggagagac
     4201 tgtaatgatc cattcatgag ctggtatgaa atcagtggga agcagaacaa cagaaggaac
     4261 tttaaaagca acaagcaatt atgtaccata tatacactgt agcaaatatt ttatatttga
     4321 gagtctttac agcttacatt tccattccat tattacaagt gatgaaaaac aaaaaattgc
     4381 aagtagtatg caaattataa atatacagtc ttcccacttc actaaccaaa ttcctacttt
     4441 ccagtgttac ttcccaattt atgcaggaaa cctcctgcaa agctgaaact gattagaaaa
     4501 ttctttatat tttaaaatag ctctttctca tttttagaga agtcaaatag ccaaccatca
     4561 aaattaagaa taaattgaat tgtcacagtc cattacagtt attgttgcta gatccacctc
     4621 atttgcagat gtccaaactt aaattcatct gttcttaaaa tgctacttaa aactttggtt
     4681 gttttcctgt aatataaaag aaaaagttaa tttatcaatt gattgaatac agtttttact
     4741 aattagttta tcaaaccaaa tactgtgaac gtaccaggtg tttacagatt taaatgcatg
     4801 ttaccataga aactattaaa gtaactagaa ctgtcaaata acaaaacggc tcatgttttt
     4861 aaaatatatg taactcattt taaaatatat taaattgtat tccaaacctg ttcttctgtt
     4921 tctgtggcac ctaggtttaa aatatgtatt aatgtgtaaa tcacaagtaa aatgaattct
     4981 aatgtacaag tttgttttaa aaagtgtatg tcaagctttt atttacacaa taaaatgtta
     5041 ttaaagatg
//
