LOCUS       NM_006393               8925 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens nebulette (NEBL), transcript variant 1, mRNA.
ACCESSION   NM_006393
VERSION     NM_006393.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Zhao,R., Liang,Z., Chu,J., Zheng,Q., Zhao,J., Tang,S., Chen,Q.,
            Huang,Y., Zhou,X. and Pan,X.
  TITLE     Downregulation of NEBL promotes migration and invasion of clear
            cell renal cell carcinoma by inducing epithelial-mesenchymal
            transition
  JOURNAL   Pathol Res Pract 254, 155068 (2024)
   PUBMED   38215565
  REMARK    GeneRIF: Downregulation of NEBL promotes migration and invasion of
            clear cell renal cell carcinoma by inducing epithelial-mesenchymal
            transition.
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Chen,L., Wang,J., Fan,X., Zhang,Y., Zhoua,M., Li,X. and Wang,L.
  TITLE     LASP2 inhibits trophoblast cell migration and invasion in
            preeclampsia through inactivation of the Wnt/beta-catenin signaling
            pathway
  JOURNAL   J Recept Signal Transduct Res 41 (1), 67-73 (2021)
   PUBMED   32635793
  REMARK    GeneRIF: LASP2 inhibits trophoblast cell migration and invasion in
            preeclampsia through inactivation of the Wnt/beta-catenin signaling
            pathway.
REFERENCE   3  (bases 1 to 8925)
  AUTHORS   Yang,Y.T., Nie,L.M., Wei,Q.F. and Li,W.Z.
  TITLE     LASP2 functions as a potential prognostic factor and therapeutic
            target in nasopharyngeal carcinoma
  JOURNAL   Eur Rev Med Pharmacol Sci 24 (18), 9416-9422 (2020)
   PUBMED   33015783
  REMARK    GeneRIF: LASP2 functions as a potential prognostic factor and
            therapeutic target in nasopharyngeal carcinoma.
REFERENCE   4  (bases 1 to 8925)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 8925)
  AUTHORS   Li,J., Hu,S., Zhang,Z., Qian,L., Xue,Q. and Qu,X.
  TITLE     LASP2 is downregulated in human liver cancer and contributes to
            hepatoblastoma cell malignant phenotypes through MAPK/ERK pathway
  JOURNAL   Biomed Pharmacother 127, 110154 (2020)
   PUBMED   32325347
  REMARK    GeneRIF: LASP2 is downregulated in human liver cancer and
            contributes to hepatoblastoma cell malignant phenotypes through
            MAPK/ERK pathway.
REFERENCE   6  (bases 1 to 8925)
  AUTHORS   Bang,M.L., Mudry,R.E., McElhinny,A.S., Trombitas,K., Geach,A.J.,
            Yamasaki,R., Sorimachi,H., Granzier,H., Gregorio,C.C. and Labeit,S.
  TITLE     Myopalladin, a novel 145-kilodalton sarcomeric protein with
            multiple roles in Z-disc and I-band protein assemblies
  JOURNAL   J Cell Biol 153 (2), 413-427 (2001)
   PUBMED   11309420
REFERENCE   7  (bases 1 to 8925)
  AUTHORS   Arimura,T., Nakamura,T., Hiroi,S., Satoh,M., Takahashi,M.,
            Ohbuchi,N., Ueda,K., Nouchi,T., Yamaguchi,N., Akai,J.,
            Matsumori,A., Sasayama,S. and Kimura,A.
  TITLE     Characterization of the human nebulette gene: a polymorphism in an
            actin-binding motif is associated with nonfamilial idiopathic
            dilated cardiomyopathy
  JOURNAL   Hum Genet 107 (5), 440-451 (2000)
   PUBMED   11140941
REFERENCE   8  (bases 1 to 8925)
  AUTHORS   Moncman,C.L. and Wang,K.
  TITLE     Functional dissection of nebulette demonstrates actin binding of
            nebulin-like repeats and Z-line targeting of SH3 and linker domains
  JOURNAL   Cell Motil Cytoskeleton 44 (1), 1-22 (1999)
   PUBMED   10470015
REFERENCE   9  (bases 1 to 8925)
  AUTHORS   Millevoi,S., Trombitas,K., Kolmerer,B., Kostin,S., Schaper,J.,
            Pelin,K., Granzier,H. and Labeit,S.
  TITLE     Characterization of nebulette and nebulin and emerging concepts of
            their roles for vertebrate Z-discs
  JOURNAL   J Mol Biol 282 (1), 111-123 (1998)
   PUBMED   9733644
REFERENCE   10 (bases 1 to 8925)
  AUTHORS   Moncman,C.L. and Wang,K.
  TITLE     Nebulette: a 107 kD nebulin-like protein in cardiac muscle
  JOURNAL   Cell Motil Cytoskeleton 32 (3), 205-225 (1995)
   PUBMED   8581976
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL157398.6.
            
            On Feb 25, 2020 this sequence version replaced NM_006393.2.
            
            Summary: This gene encodes a nebulin like protein that is
            abundantly expressed in cardiac muscle. The encoded protein binds
            actin and interacts with thin filaments and Z-line associated
            proteins in striated muscle. This protein may be involved in
            cardiac myofibril assembly. A shorter isoform of this protein
            termed LIM nebulette is expressed in non-muscle cells and may
            function as a component of focal adhesion complexes. Alternate
            splicing results in multiple transcript variants. [provided by
            RefSeq, Mar 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). This isoform (1) is
            also known as the sarcomeric isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y16241.1, BC110453.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377122.9/ ENSP00000366326.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-187               AL157398.6         167745-167931       c
            188-259             AL157398.6         167578-167649       c
            260-364             AL157398.6         160465-160569       c
            365-475             AL157398.6         158717-158827       c
            476-586             AL157398.6         151414-151524       c
            587-688             AL157398.6         140360-140461       c
            689-790             AL157398.6         139284-139385       c
            791-904             AL157398.6         130333-130446       c
            905-1009            AL157398.6         128860-128964       c
            1010-1114           AL157398.6         123165-123269       c
            1115-1222           AL157398.6         121015-121122       c
            1223-1333           AL157398.6         115878-115988       c
            1334-1444           AL157398.6         111359-111469       c
            1445-1555           AL157398.6         106133-106243       c
            1556-1666           AL157398.6         102093-102203       c
            1667-1777           AL157398.6         101816-101926       c
            1778-1882           AL157398.6         99150-99254         c
            1883-1975           AL157398.6         97067-97159         c
            1976-2068           AL157398.6         93828-93920         c
            2069-2161           AL157398.6         90044-90136         c
            2162-2254           AL157398.6         88220-88312         c
            2255-2347           AL157398.6         86245-86337         c
            2348-2452           AL157398.6         84559-84663         c
            2453-2624           AL157398.6         83389-83560         c
            2625-2717           AL157398.6         80426-80518         c
            2718-2867           AL157398.6         79130-79279         c
            2868-2974           AL157398.6         57822-57928         c
            2975-8925           AL157398.6         50593-56543         c
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.31"
     gene            1..8925
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /note="nebulette"
                     /db_xref="GeneID:10529"
                     /db_xref="HGNC:HGNC:16932"
                     /db_xref="MIM:605491"
     exon            1..187
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..3151
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /note="isoform 1 is encoded by transcript variant 1;
                     actin-binding Z-disk protein; LIM-nebulette; LIM and SH3
                     protein 2"
                     /codon_start=1
                     /product="nebulette isoform 1"
                     /protein_id="NP_006384.1"
                     /db_xref="CCDS:CCDS7134.1"
                     /db_xref="GeneID:10529"
                     /db_xref="HGNC:HGNC:16932"
                     /db_xref="MIM:605491"
                     /translation="MRVPVFEDIKDETEEEKIGEEENEEDQVFYKPVIEDLSMELARK
                     CTELISDIRYKEEFKKSKDKCTFVTDSPMLNHVKNIGAFISEAKYKGTIKADLSNSLY
                     KRMPATIDSVFAGEVTQLQSEVAYKQKHDAAKGFSDYAHMKEPPEVKHAMEVNKHQSN
                     ISYRKDVQDTHTYSAELDRPDIKMATQISKIISNAEYKKGQGIMNKEPAVIGRPDFEH
                     AVEASKLSSQIKYKEKFDNEMKDKKHHYNPLESASFRQNQLAATLASNVKYKKDIQNM
                     HDPVSDLPNLLFLDHVLKASKMLSGREYKKLFEENKGMYHFDADAVEHLHHKGNAVLQ
                     SQVKYKEEYEKNKGKPMLEFVETPSYQASKEAQKMQSEKVYKEDFEKEIKGRSSLDLD
                     KTPEFLHVKYITNLLREKEYKKDLENEIKGKGMELNSEVLDIQRAKRASEMASEKEYK
                     KDLESIIKGKGMQAGTDTLEMQHAKKAAEIASEKDYKRDLETEIKGKGMQVSTDTLDV
                     QRAKKASEMASQKQYKKDLENEIKGKGMQVSMDIPDILRAKRTSEIYSQRKYKDEAEK
                     MLSNYSTIADTPEIQRIKTTQQNISAVFYKKEVGAGTAVKDSPEIERVKKNQQNISSV
                     KYKEEIKHATAISDPPELKRVKENQKNISNLQYKEQNYKATPVSMTPEIERVRRNQEQ
                     LSAVKYKGELQRGTAISDPPELKRAKENQKNISNVYYRGQLGRATTLSVTPEMERVKK
                     NQENISSVKYTQDHKQMKGRPSLILDTPAMRHVKEAQNHISMVKYHEDFEKTKGRGFT
                     PVVDDPVTERVRKNTQVVSDAAYKGVHPHIVEMDRRPGIIVDLKVWRTDPGSIFDLDP
                     LEDNIQSRSLHMLSEKASHYRRHWSRSHSSSTFGTGLGDDRSEISEIYPSFSCCSEVT
                     RPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSMRSMQHSPNLRTYRAMYDYSAQDEDE
                     VSFRDGDYIVNVQPIDDGWMYGTVQRTGRTGMLPANYIEFVN"
     exon            188..259
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            260..364
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            365..475
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            476..586
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            587..688
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            689..790
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            791..904
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1009
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1114
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1222
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1333
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1444
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1555
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1666
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1777
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1882
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1975
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2068
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2161
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2254
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2347
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2452
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2624
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2717
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2867
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..2974
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     exon            2975..8925
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7723..7728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /note="hexamer: AATATA"
     polyA_site      7740
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /note="major polyA site"
     regulatory      8907..8912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
                     /note="hexamer: AATAAA"
     polyA_site      8925
                     /gene="NEBL"
                     /gene_synonym="bA165O3.1; C10orf113; LASP2; LNEBL"
ORIGIN      
        1 atttctgcca gagcaccaga gacgtcagca tctcaagggc actgtggtat ggaaaaggac
       61 gccacatgag taaattttaa aaatataaat attttaaagg gtaaaaatga gggtccctgt
      121 atttgaggat ataaaagatg aaactgaaga agaaaagata ggggaagaag aaaatgaaga
      181 agaccaggtc ttctataagc ctgttattga agacttaagc atggaattgg ccagaaaatg
      241 cacggaactc attagcgata tccgttataa agaagagttt aaaaagtcca aggataagtg
      301 tacatttgtg actgacagtc ctatgctaaa ccatgtaaaa aatatcggtg cttttatttc
      361 tgaggcaaaa tacaaaggca ccattaaagc tgacctttct aattctcttt ataagcggat
      421 gccagccaca attgacagtg tttttgcagg agaagttaca cagctccaga gtgaggtggc
      481 ctacaagcag aaacatgatg ctgccaaagg attctcagat tatgcccaca tgaaggagcc
      541 ccctgaggtt aaacatgcca tggaggtcaa taaacaccag agtaatattt cttataggaa
      601 agacgtgcag gacacccaca cgtacagtgc agaacttgac cgaccagaca tcaagatggc
      661 aacccagatc tctaagatca taagcaatgc agaatacaag aaaggacaag gaataatgaa
      721 taaagagccc gctgtaattg gaagaccaga ttttgaacat gccgtggaag cttctaaact
      781 ttctagtcaa attaaataca aagaaaaatt tgataatgaa atgaaggata agaaacatca
      841 ttacaatcct cttgaaagtg cttcttttag gcagaatcag cttgctgcta cactggcgag
      901 caatgtgaag tacaagaaag acattcaaaa tatgcatgat ccagtttcag atctcccaaa
      961 tttgttgttt ttagaccatg ttttgaaagc cagcaaaatg ctcagcggcc gagaatataa
     1021 aaagctcttt gaggaaaaca aaggaatgta tcattttgat gcagatgctg tggaacatct
     1081 gcaccataaa ggcaatgccg tcctccaaag tcaggtgaaa tataaagaag aatatgagaa
     1141 aaataaggga aagccaatgc ttgaatttgt tgagacacca tcatatcaag cttcaaagga
     1201 ggctcaaaag atgcaaagtg aaaaagttta caaagaggat tttgagaagg agattaaagg
     1261 aaggtcatca ctggatttag acaagactcc agaattttta catgtaaagt acatcaccaa
     1321 ccttctgagg gagaaagaat ataaaaaaga tttggaaaat gagataaaag ggaaaggaat
     1381 ggaacttaat tcagaagttc ttgatatcca aagagcaaag cgagcctctg aaatggcaag
     1441 tgagaaagaa tacaagaaag acctggagtc aataattaaa gggaaaggaa tgcaagctgg
     1501 cactgacacc cttgaaatgc agcatgccaa gaaggctgca gagatagcga gtgagaaaga
     1561 ctataaaaga gatctggaga ctgaaattaa agggaaaggg atgcaggtga gcacagacac
     1621 tcttgatgtc cagagagcta agaaagcatc cgagatggcc agccagaaac aatacaagaa
     1681 ggacttagaa aatgaaatta aagggaaagg aatgcaagtg agcatggata tcccagatat
     1741 ccttcgagcc aagaggacat ctgaaatcta tagccagaga aagtataaag atgaagcaga
     1801 gaagatgctt tctaactatt ctaccatagc agatactcct gaaattcaga gaattaagac
     1861 aactcaacaa aacattagtg cggtatttta taagaaagaa gtgggagctg gcactgcagt
     1921 gaaagatagc ccagagatcg aacgagtgaa gaaaaatcag cagaatatta gttcagtgaa
     1981 atacaaagaa gagattaaac atgcaacagc catttctgat cctccagaac taaagagagt
     2041 taaagaaaac cagaagaaca tcagcaatct ccagtataaa gagcaaaact acaaggccac
     2101 tccggtaagc atgaccccgg agatagagag agtgaggcga aaccaggagc agctgagtgc
     2161 ggtaaaatat aagggagaac ttcaacgggg aactgcaatt tctgatccac cagagctgaa
     2221 gagggcaaaa gaaaaccaga aaaacatcag caatgtttat tacagaggtc agctgggaag
     2281 agctaccact ttaagtgtaa ctcctgaaat ggaaagagtg aagaagaatc aagaaaatat
     2341 tagctcggta aaatataccc aggaccataa acagatgaaa ggtagaccaa gtctgatttt
     2401 agatacacct gctatgagac atgttaaaga agcacaaaat catatttcaa tggtaaaata
     2461 ccatgaagat tttgaaaaaa caaaggggag aggctttact cccgtcgtgg acgatcctgt
     2521 gacagagaga gtgaggaaga acacccaggt ggtcagcgat gctgcctata aaggggtcca
     2581 ccctcacatc gtggagatgg acaggagacc tggaatcatt gttgacctca aagtttggcg
     2641 cacagatcct ggctccatct tcgaccttga tcccctggaa gacaatattc agtctagaag
     2701 tctccatatg ctctctgaaa aggcgagtca ctataggcga cactggtctc gatcccattc
     2761 cagcagtact ttcggtacag gtctcggaga cgacaggtca gaaatctccg agatttaccc
     2821 tagcttttca tgctgcagtg aggtaacaag accgtctgat gaaggagcac ctgttcttcc
     2881 cggagcctat cagcaaagcc attcccaagg ctatggctac atgcaccaga ccagtgtgtc
     2941 atccatgaga tcaatgcagc attcaccaaa tctaaggacc taccgagcca tgtacgatta
     3001 cagtgcccag gatgaagacg aggtctcctt tagagacggc gactacatcg tcaacgtgca
     3061 gcctattgac gatggctgga tgtacggcac agtgcagaga acagggagaa caggaatgct
     3121 cccagcgaat tacattgagt ttgttaatta attatttctc cctgcccttt gagctttatt
     3181 ctaatgtatc ccaaacctaa tctttttaaa agatagaaga tacttttaag acaacttggc
     3241 cattatttta caatgatgta tccttccttt gacaattaga cacacaggta ccaggaagaa
     3301 ggaatgacct ctgggctgaa aacagcagca ttttcagtaa ttcctacaaa caaaaatctt
     3361 tgtgtctgga cacctggtgc tgctaattgt gttcatggtt tcctttgatt ggctattgaa
     3421 cccttctggg aaatgtattt ttgtagactt taatagagaa gttgattgtc ccttaaatgt
     3481 agtgtgtgtt tgaaacttct tagctgtcac tttggaatca ccccaagcca attctcttaa
     3541 ctctgtaatg cagccaataa tttcaaaccc gttttgcttt tgagtcatga ggcaatttcc
     3601 aatattagtg aaaattgccc aatataataa gtgtaaacag tggcagaagg acagtctggt
     3661 taaaattata ttgactggtg gccttaggga tctagaaact tctactaaac agagaaattt
     3721 ccttgttccc taggctgact ggtatctatt tatttctcat ttgtaccaag gcatctccta
     3781 ctctccattt atattctatg gacccaagtc tatgctcagt tccacagaat gtcaggacca
     3841 aataacttca cagctactct gcaaagggca aattataatg tcattgatat aatttcccta
     3901 gtagcattta ccctgttgca tgtcatgtag attcaagctt ctgtaacata ggcagctgca
     3961 ctgcgcgttc ctattattga agcaaaaagg gtgactgata cctaaaagcc ctttcttcct
     4021 ctagtcgcca gctcatcaga aaaacatact ttgaaaagat gcttgagatt ttcctgctgc
     4081 atcgcactct agttttgaag gatttacatc ttaggaaata acatgtatac tctagtaaat
     4141 aagcgattta ggtgttccat tgaacagctt tgattaactt aatgccacca ttgatttcaa
     4201 agtgaagaaa atgtaacaga agccagtgaa gcaatggaag ctggagtgtg actggaaaaa
     4261 tactcagcaa acaaagttac caattccata cagagatgat ctggtatctt cttttggaaa
     4321 atggtattca aattctggaa tggaaatcta gccaccaaaa cgggttaatc aaaagacgtc
     4381 cttttccatt tttttttgct tttattttct aaatcatttt taagggaatg aaacaggaat
     4441 gtcatcagag attttttagt acaggcccaa gagcctgttc tctaagaaag aaattgttgc
     4501 catgttttga ttttcgaata agtgactttg caggctttat gctagccctt gctggtgggt
     4561 cttgaaattt catccagagt ctgcagtcca ggtcaccaag ccagcggcac ccgtcggcaa
     4621 ccctgtgttt ttctgattgt gccgtttact gtgacctgca acggggtggc attcacttag
     4681 ggtctgactt cacagctatg acaaaaccga aaaagcaaaa ctgcaaaaaa gtactaagat
     4741 gtacgggtct tggggatatc tgccttatat gttatattca aggaaattaa caaaacatcc
     4801 tgtaaaacat cgtttaagga aacgtttact agtccaaagg ccaaagctaa tttatttcca
     4861 ctttagaaaa gttagcacat gcttttgaaa atctgtgatt tcattttatt aggctaaaag
     4921 ggtaaatagg ctttattaca ctgaagctgc atctatatgt cactgacata aagttgaaaa
     4981 aataaatgca ggcaaataac tagagacttc ttttaagggg gtttggctgg ttttctctca
     5041 ctgaaatggc cagtcgtgat taaagtgata aaaccccata tctgttttgg tatattgtac
     5101 acaaacctac aaaaataaac tgaacttgca atatttttgc aaaaaaatct gtcgttaaaa
     5161 ctgaggataa aatacctgct caattttatt ttactaagta tatatttaca tttcacccag
     5221 gcaggccatt ttcttttgtg attataagaa agagtagttg ttgattaaat tttcagacta
     5281 aatataggac aggtacaatt ttggataaat agcacattta taagaaccgc aatgaaaact
     5341 gacttgaaat aatgcttgta atcaggaaag taatttcatc caccgatttc aaaaccagat
     5401 tcactgagca taaaagtcaa tacatatttg aggaataagt ctcctaaaat tttaagcttc
     5461 acgtaataat gtttgcatag caaaatattt ctgcttcaag cctttaggaa ttaagatctg
     5521 atcagaattt aactaaaggg tagttgtttt acaatgaaga ctaaaactga acaagatgtt
     5581 gcatgctctt gaggccataa tttggtagtg ttggcagttg ttaataaagc ttgtcaggat
     5641 gttaagcatc tcaggagaaa tattggaaaa ttatatgtat aaaaccaaag tgctattttt
     5701 aaaagcatca tttaaaaaaa aatgacatgc ctgaacaact tttccacttt ccacgtgctt
     5761 ccctcccacc tttggtttgg caacaggtat ctcgtgcatg aagctgacag ctaaagaaga
     5821 ttttaaaaat tgagttaaag atgactgtgt aaatgtccaa gcacagagag catgcacctg
     5881 actttctaaa gtttgatgtg ttctcaagcc tgacagaagc acaaggaaca gtttgataca
     5941 cttttaaaag gttctgaaaa caaagctgta tagggatcct ctctctcttg agcaaagtat
     6001 agcaacagaa tatattgctt ttgttgtaag cttttgtagt acatgttttt actaataatt
     6061 cttgttctct agaaagcttt ctatttctaa cctatggcaa aatgaatcct tcatgtcttc
     6121 ttgttattgt ttacacactt gcagtgtagc ccagtttgaa atatttattt ggttatcaac
     6181 tgcccatgga ggaggctctt gatgatccca ggtctcctcg acctccatac accacacagg
     6241 catttgtaag cacagtttcc acaagcacct tgtaggaata tggataagat tagaccagcc
     6301 cctctctgtc cactgggttt atttcttgaa gaagatgcag atctggtttt tccaatgtgc
     6361 cacagtcttt ccttatcctc tccatgctga gcttgacaac actctgggaa tgaggaacaa
     6421 gactttttct aaaaagatag tggaagttca agggatgtac ctcgttttca ggttcatcca
     6481 tctccagtgg aatgttttca ataaaagatg aagaaaatgt gtgtgatctt taataacaca
     6541 tccctataga aagtggataa aagatatacc aaaactgtaa tacagatata tacaaatata
     6601 ggtgcctttt tgattactct tgtttgtcta gtatgctctt ggaaagaaaa ccaagcaagc
     6661 aagttgctgc ctattctata gtaatatttt attacacatg attgatattt ttgtggtagg
     6721 gaagtgggat gctcctcaga tattaaaggt gttagctgat tgtattttat ctctaaagat
     6781 ttagaacttt agaaaatgcc gacttcttcc atctatttct gaaaggttct ttgtggattt
     6841 atatagagtt gagctatata aacattaact ttagatttgg gatttaaaat gcctattgta
     6901 agatagaata attgtgaggc tggattcact acacaagatg aacttcactt cataaattaa
     6961 ttatacctta gcgatttgct tctgataatc taaaagtggc tagattgtgg ttgttttggt
     7021 taaggtgata tggaggtggg agagctttta gttaagtaag aagctatgta aactgacaag
     7081 gatgctaaaa taaaagtctc tgaagtattc catgcctttt ggaccctttc ctcgcaacta
     7141 actgtcaact gttgatcaaa aaagtcaagg cattgtatgt tgcttctgtg gttattattc
     7201 tgtgatgctt agactacttg aacccataaa cttggaagaa tctttgagca aattttctca
     7261 gttgtctgta tgacttcagt atattcctgg gaatgccata ggattttttg tgcttgatac
     7321 atggtatcca gtttgcatag tatcacttct ttgtaatcca gttgctgtta agaatgatgt
     7381 actttaaagg aaaagagaaa actgcatcac agtcccattc tccagtgtcc atgcaatgaa
     7441 ttgctgagca tttaggaagc agcaccaagt ctattacagg catggtgtga aacttgatgt
     7501 ttgacctgtg atcaaaattg aaccattgta cagtttggct tctgtttgct tcaaaatatg
     7561 tagaattgtg gttgatgatt aatttgcgag actaactttg agagtgtaac agttttgaag
     7621 aaaacattga atgttttgca aatgaagggg cttcacggaa tgttacaatg ttactaatat
     7681 aatttggctt ttgttatgca aattgttaac accagctatt aaaatatatt ttagtagaaa
     7741 tgctttaatt catatttttt tcctctacac tgtgaatctt taagccttgg tggactagag
     7801 caacatcgtg ctgcccaaag gactaaccta tgcaaactag ttcacatttt agtggatgtc
     7861 gcagttaatg tgtaataaga cattatttcc cctgcataat gtacaacagc attgaaatga
     7921 cacattaagc ctagcatcac attgtatagt acagtcactc acaaaccctt caaggctacc
     7981 ctaatcatta acattaatat ttgtttaaaa gcaaatcacc gatttatcta ttgaaactac
     8041 ttaaatgacg gcaaaccagg aatgacagat ggctgtgtca gcaatggctt taatgtgttc
     8101 cctgcaagtg gtctcctatg atagaactgc gttctcaaat gcactctctt cagggtctta
     8161 atattctgtg ttttctctct gtatttgtaa aacattataa cacattaatt tcctatctct
     8221 acacatttgg tttgcttaaa taaatgcagg atataaaaaa aatggttcac ttcttggctc
     8281 tcaccgtggt ttcttggagc atgggttgtt agatgcaagc aatgcaccct aataataccc
     8341 cgggtctgag atttaacatg acaactcaca tcaaatcgca tcagaggtgt gtgctgcctt
     8401 cagtgcattt acattggtga atcagtcaag atattttcct cccccaaata aacttagttg
     8461 taagtgataa caatattatg cttctccaag ctcagtatct ttctgatttt atatcaaagt
     8521 accgcaacaa tgcatcattg tagttaattt atttcaagaa taaattcctc atatgtcctc
     8581 aatagtacaa ttctaatttt cttctattca taagatgaaa gaaatggttt ggagcataga
     8641 atagaaagtg cacaaattga gtacataaaa tgggaagcaa ctgatttctc agctaagaaa
     8701 ggctcattta tcacagaaca caattgcttt tctcccccca ctacgcttcc cataattgaa
     8761 aaagtgagtc cctatttttc acactcatat aaatctatgc gatttggatg ctagtcttat
     8821 tgtattattt tgtaaaactt tctctttggc tcataatcct tcctaattgt aaattgataa
     8881 actttgcgga tgacatctgc tcgtagaata aacacttctt ccaaa
//
