LOCUS       NM_021075               5725 bp    mRNA    linear   PRI 26-FEB-2025
DEFINITION  Homo sapiens NADH:ubiquinone oxidoreductase subunit V3 (NDUFV3),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_021075 XM_003119831 XM_003119832
VERSION     NM_021075.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5725)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   4  (bases 1 to 5725)
  AUTHORS   Signes,A. and Fernandez-Vizarra,E.
  TITLE     Assembly of mammalian oxidative phosphorylation complexes I-V and
            supercomplexes
  JOURNAL   Essays Biochem 62 (3), 255-270 (2018)
   PUBMED   30030361
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5725)
  AUTHORS   Guo,R., Zong,S., Wu,M., Gu,J. and Yang,M.
  TITLE     Architecture of Human Mitochondrial Respiratory Megacomplex
            I2III2IV2
  JOURNAL   Cell 170 (6), 1247-1257 (2017)
   PUBMED   28844695
REFERENCE   6  (bases 1 to 5725)
  AUTHORS   Zakharova,N.V. and Zharova,T.V.
  TITLE     Kinetic mechanism of mitochondrial NADH:ubiquinone oxidoreductase
            interaction with nucleotide substrates of the transhydrogenase
            reaction
  JOURNAL   Biochemistry (Mosc) 67 (12), 1395-1404 (2002)
   PUBMED   12600270
REFERENCE   7  (bases 1 to 5725)
  AUTHORS   Scanlan,M.J., Gordan,J.D., Williamson,B., Stockert,E., Bander,N.H.,
            Jongeneel,V., Gure,A.O., Jager,D., Jager,E., Knuth,A., Chen,Y.T.
            and Old,L.J.
  TITLE     Antigens recognized by autologous antibody in patients with
            renal-cell carcinoma
  JOURNAL   Int J Cancer 83 (4), 456-464 (1999)
   PUBMED   10508479
REFERENCE   8  (bases 1 to 5725)
  AUTHORS   Loeffen,J.L., Triepels,R.H., van den Heuvel,L.P., Schuelke,M.,
            Buskens,C.A., Smeets,R.J., Trijbels,J.M. and Smeitink,J.A.
  TITLE     cDNA of eight nuclear encoded subunits of NADH:ubiquinone
            oxidoreductase: human complex I cDNA characterization completed
  JOURNAL   Biochem Biophys Res Commun 253 (2), 415-422 (1998)
   PUBMED   9878551
REFERENCE   9  (bases 1 to 5725)
  AUTHORS   de Coo,R.F., Buddiger,P., Smeets,H.J. and van Oost,B.A.
  TITLE     Molecular cloning and characterization of the human mitochondrial
            NADH:oxidoreductase 10-kDa gene (NDUFV3)
  JOURNAL   Genomics 45 (2), 434-437 (1997)
   PUBMED   9344673
REFERENCE   10 (bases 1 to 5725)
  AUTHORS   Sled,V.D. and Vinogradov,A.D.
  TITLE     Kinetics of the mitochondrial NADH-ubiquinone oxidoreductase
            interaction with hexammineruthenium(III)
  JOURNAL   Biochim Biophys Acta 1141 (2-3), 262-268 (1993)
   PUBMED   8443212
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI829092.1, BF311986.1,
            BC021217.2, BU629860.1, BC033766.1, CF123536.1 and AP001629.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_021075.3.
            
            Summary: The protein encoded by this gene is one of at least
            forty-one subunits that make up the NADH-ubiquinone oxidoreductase
            complex. This complex is part of the mitochondrial respiratory
            chain and serves to catalyze the rotenone-sensitive oxidation of
            NADH and the reduction of ubiquinone. The encoded protein is one of
            three proteins found in the flavoprotein fraction of the complex.
            The specific function of the encoded protein is unknown. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.198344.1,
                                           SRR1163657.234349.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000354250.7/
                                                  ENSP00000346196.2
            RefSeq Select criteria             :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BI829092.1         45-59
            16-511              BF311986.1         2-497
            512-1261            BC021217.2         540-1289
            1262-1520           BU629860.1         24-282              c
            1521-1572           BC033766.1         425-476
            1573-1590           BC033766.1         478-495
            1591-2070           CF123536.1         152-631
            2071-2089           BC033766.1         976-994
            2090-5725           AP001629.1         59661-63296
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..5725
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="NADH:ubiquinone oxidoreductase subunit V3"
                     /db_xref="GeneID:4731"
                     /db_xref="HGNC:HGNC:7719"
                     /db_xref="MIM:602184"
     exon            1..73
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..1447
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /EC_number="7.1.1.2"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; NADH-ubiquinone oxidoreductase flavoprotein 3,
                     10kD; complex I 10kDa subunit; NADH-ubiquinone
                     oxidoreductase 9 kD subunit; complex I, mitochondrial
                     respiratory chain, 10-kD subunit; NADH dehydrogenase
                     [ubiquinone] flavoprotein 3, mitochondrial; mitochondrial
                     NADH oxidoreductase-like protein; complex I-9kD; renal
                     carcinoma antigen NY-REN-4; NADH-ubiquinone oxidoreductase
                     9 kDa subunit; NADH dehydrogenase (ubiquinone)
                     flavoprotein 3, 10kDa"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] flavoprotein 3,
                     mitochondrial isoform a precursor"
                     /protein_id="NP_066553.3"
                     /db_xref="CCDS:CCDS33572.1"
                     /db_xref="GeneID:4731"
                     /db_xref="HGNC:HGNC:7719"
                     /db_xref="MIM:602184"
                     /translation="MAAPCLLRQGRAGALKTMLQEAQVFRGLASTVSLSAESGKSEKG
                     QPQNSKKQSPPKNVVEPKERGKLLATQTAAELSKNLSSPSSYPPAVNKGRKVASPSPS
                     GSVLFTDEGVPKFLSRKTLVEFPQKVLSPFRKQGSDSEARQVGRKVTSPSSSSSSSSS
                     DSESDDEADVSEVTPRVVSKGRGGLRKPEASHSFENRAPRVTVSAKEKTLLQKPHVDI
                     TDPEKPHQPKKKGSPAKPSEGRENARPKTTMPRSQVDEEFLKQSLKEKQLQKTFRLNE
                     IDKESQKPFEVKGPLPVHTKSGLSAPPKGSPAPAVLAEEARAEGQLQASPPGAAEGHL
                     EKPVPEPQRKAAPPLPRKETSGTQGIEGHLKGGQAIVEDQIPPSNLETVPVENNHGFH
                     EKTAALKLEAEGEAMEDAAAPGDDRGGTQEPAPVPAEPFDNTTYKNLQHHDYSTYTFL
                     DLNLELSKFRMPQPSSGRESPRH"
     exon            74..194
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /inference="alignment:Splign:2.1.0"
     exon            195..1289
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..5725
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1505..1510
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="hexamer: AATAAA"
     polyA_site      1526
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="major polyA site"
     regulatory      2069..2074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="hexamer: ATTAAA"
     polyA_site      2096
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
     regulatory      4307..4312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="hexamer: ATTAAA"
     polyA_site      4327
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
     regulatory      5634..5639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="hexamer: TATAAA"
     polyA_site      5658
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
     regulatory      5704..5709
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
                     /note="hexamer: AATAAA"
     polyA_site      5725
                     /gene="NDUFV3"
                     /gene_synonym="CI-10k; CI-9KD"
ORIGIN      
        1 gcttggtgcg cccgctgtca ccgccatggc tgccccgtgt ttgctgcggc aaggacgagc
       61 cggggcgctg aagactatgc tccaggaagc ccaggtgttt cgaggacttg cttctacggt
      121 ttctttgtct gcggaatcag ggaagagtga aaagggtcag ccacagaatt ccaagaagca
      181 aagtccacca aaaaatgtag tggaaccaaa ggagaggggc aagctcctag ccacccagac
      241 agcagctgaa ttgtctaaaa acttatcttc acccagttct tacccgccag ctgtgaataa
      301 gggcaggaag gtagctagtc ccagtcccag tggcagcgtg ctattcacag atgaaggggt
      361 tccgaaattt ttgtcaagaa agactttggt agagtttcca cagaaagttc tgtctccatt
      421 cagaaaacag ggctctgatt cagaagctcg tcaggtgggt cggaaagtga cgtcgccttc
      481 gtcttcatcc tcttccagct cctctgattc tgaatctgat gatgaggctg acgtttcaga
      541 ggtcactcct cgagtggtga gcaaaggcag aggggggctt cgaaaaccag aggcctctca
      601 ttcctttgaa aacagagccc cccgagttac agtatcagca aaagagaaaa ccttgctgca
      661 gaagccgcat gtggacatta ctgatccaga gaagccccac cagccaaaga agaaagggtc
      721 ccctgctaag ccatcagaag gcagggaaaa tgcgagacca aaaaccacaa tgcccagatc
      781 tcaagtagat gaagagtttt tgaagcaaag tttaaaggaa aaacaattgc agaaaacatt
      841 tagattaaat gaaatagata aagaaagcca aaagccattt gaagttaaag gacccttacc
      901 tgtccacaca aaatcagggt tgtctgcgcc accgaagggc agcccagcgc ctgctgtgtt
      961 ggcagaagag gccagagcag aggggcagct gcaagccagt cctcctgggg cggcagaggg
     1021 gcatctggaa aaacccgtgc cagagcccca gcgcaaggcg gcccctcccc tgcccagaaa
     1081 ggaaacctca gggacgcagg gaatagaagg ccacctgaag ggtggacagg caatcgtgga
     1141 agatcagata ccaccaagca atttggagac agttcctgtt gagaataacc acggtttcca
     1201 tgaaaagaca gcagcgctga agcttgaggc cgagggcgag gccatggaag atgcagccgc
     1261 gccaggggac gaccgaggcg gcacacagga gccagcccca gtgcctgctg agccgtttga
     1321 caacactacc tacaagaacc tgcagcatca tgactacagc acgtacacct tcttagacct
     1381 caacctcgaa ctctcaaaat tcaggatgcc tcagccctcc tcaggccggg agtcacctcg
     1441 acactgaggg ccctcggtgt gaagatgaac cttccaccgt cttcactgca tcctggagtg
     1501 caaaaataaa atccactcaa gagtcacaag gcccgctgtg cataatcggt ttcactttta
     1561 cctttttttt tttttttttt tttttgagac agggtctcac tctgtcaccc aggctggagt
     1621 gcagtggcac attctcggct cactgcaact tccgcctcct gggttcaagt gattctccca
     1681 cctcagcctc ccaagtaggt gggattacag gtactcacca ccaggtccag ctaacttttg
     1741 tatttttagt agagacaggg tttcaccatg ttggccaggc tggtctcgaa ctcctgacct
     1801 cagatggtct gcccacctcc gcctcccaaa gtgctgggat tacaggcgtg agccactgcg
     1861 cccggccact ttcacacttt ttacagtgag tggtgaatta gcaacagtaa cactgattat
     1921 ccaacatata ttttggaata tctactatgt gcaaggaatt tttcttaaac tctaaggtta
     1981 tgaatcactg ggcaaatcca tataattaga gaattttaag tgctttagag cggtgtgatt
     2041 ctactgtgct cagcctagtc aatttcgcat taaactgatt atcagctgag tatcaataat
     2101 gtaatagtga cttcttttat tttttttgag atggagtctc tgtttcccag gctggagtcc
     2161 agcggcacaa tctaaactca ctgcaacctc cgcctcctgg gttcaagtga ttctcctgcc
     2221 ttacttagcc tcttgagtag ctggtactac aggctcacgc caccatgcct ggctaatttt
     2281 ttttgtattt ttagtagaga tggggtttca ccatcttggc caggctggtc ttaaactcct
     2341 gacctcatga tctacctgcc tcagcctccc aaaatgctgg cattataggc gtgagccact
     2401 gcacccagcc agtaatagtg acttctaatc ctacagccct ggtttttgat cattagaaac
     2461 aggagttttg gccaggtgca gtggataatt cctgtaatcc cagcgctttg ggagggcgag
     2521 gcgggtggag cacctgaggt caggagttga gaccggcctg gccaacatag tgaaacccca
     2581 tctctactaa aaatacaaaa atcagccagg catgctagtg ggtacctgta atcccagcta
     2641 ctcaggaagc tgaggcagga gaatcatttg aacctgggag gcggaggttg cagtgagcaa
     2701 gatcgtgcca ttgtactcca gcctgggcaa caggagtgaa actcaatcaa aaaaaaggaa
     2761 caggaatgtt actaatttta actactgcat tgcaaacaaa ttgtattgca gtattttacc
     2821 atcagaaaag gaagggaatt gttttgtttg cctctgttag atattttata taaacttgag
     2881 gacattttca ttggtgagat tcggaagagg tgaagtttct tgcagtacag ggacgaagta
     2941 ggaagaggtg aagtttcgtg cagtacaggg acggagtagg aagaggtgaa gtttcgtgca
     3001 gtacagggac ggagtaggaa gaggtgaagt ttcgtgcggt gcagggacgg agtaggaaga
     3061 ggtgaagttt cgtgcggtgc agggacggag taggaagagg tgaagtttcg tgcggtgcag
     3121 ggacggagta ggaagaggtg aagttttgtg cggtgcaggg acgaagttgc tccccgctgg
     3181 ccacaggcac tcaatgagct attttaccta tttagggttt ctgggttggc caagtaacct
     3241 cccatccctt gtgatagata actttaagtc acacctttgg gggcaatgtg gggggctcct
     3301 gtgcatcaca tggagcacgc tcccccagca ctctgcagag acagaccccc agtctgactt
     3361 agagaaagta accaatgtag gtgagggcca gggcacggtg gcttacgcct gtaatcccag
     3421 cactttggga agccaaggcg gatggatcac ttgaggtcag gagtttgaga ccagcctggc
     3481 caacatggtg aaaccctgtg tctactaaaa atacaaaaat tagccaactg tggtggtgcg
     3541 tgcctgtaat cccagctatt caagaggctg aggcaggaga atcgcttgaa cccgggaggc
     3601 ggggtttgca gtaagccgag atcatgccat tgcattcagt ctgggtgaca gcgtgagact
     3661 gcatctcaaa aaaaaatttt tttttttaaa tatcaaaatt gaaaactagt attgtggtca
     3721 tctgcctcaa attgtgttac cacacgggag acttagcaac tcagccagat tcttctaaac
     3781 acatttttta taataaaagt ggtaaaacta cattaccaaa aagaaaatag taaacttacc
     3841 taaaatccac tatgctaacc cacccttttt tttttttttt tttttttttt ttttttgaga
     3901 cagcctcact cttgcccgga ggagtggtgc gatctcagct cactgcagcc tcgtcctccc
     3961 aggcccaagc aatcctccca tctcagccca ctttgtagct gggactgtag gcactggcca
     4021 ccatgtccgc ctaatttttc tattttttgt agagacaggg ttcaagagat ctgcctgcct
     4081 cggccccaga aagttctggg attgcaggcg tgagccccca ttcccagcca ttaacatctt
     4141 acaggattcc ccagtggtgg tggtaggtgt aaccatttta atgtacctac aacttttcta
     4201 tcctgctatt tgcatttttt ggtttttcat ttaatagttc tcaagctggg atccgtggaa
     4261 ctactgtgat gggcggtgat gctttggaaa tggaaattcc cttaatatta aaatgaacat
     4321 gaatatattt tgatttgcat atatttttaa gataaagcat gagacttgta taaagttctt
     4381 tcctggctgg gcgcggtggc tcatgcctgt aatccctgca ctttggaagg ctgaggcaga
     4441 tggatcacct gaggttggga gtttgagacc agcctgacta acatggtgaa accccctctc
     4501 tactaaaatt acaaaattag ccgggcgtgt tggtgcatgc ctgtaatccc agctactcaa
     4561 gaggctgagg caggaaaatc acttgaaccc aggaggcaga ggctgcagtg agtggagatt
     4621 gcagcatcgc actacagcct gggcaacaac agcaaaactc cgtctcaaaa aaataaagtt
     4681 cttcccttag aactagtcct gttgggctag tctttctggg attttggttt actctcctcc
     4741 acccattgga agggcttaaa accgaatagc cagcaccaag cacagctctg cattttgtag
     4801 ctatcactgc agatgatttc ttgatagtcc atctggtggc aatacagtgg tcacccaagg
     4861 ggccattgtt tgggtctatg tgatttccca cgttctgttg gaggccgggc gcagtgcctc
     4921 acgcctgtaa tcccagcact ttgggaggct gaggtgggca gatcacgagg tcaggagatc
     4981 gagaccgtcc tggctaacac ggtgaaaccc tgtctctact aaaaatacaa aaaattggcc
     5041 aggtgcggtg gctcacgcct gtaatcccag cactttggga ggctgaggtg ggcagatctc
     5101 gaggtcagga gatcgagacc atcctggata acacggtgaa accccgtctc tattaaaaat
     5161 acaaaaaatt agccgggcat ggtagcgggc gcctgtagtc ccagctactt gggaggctga
     5221 ggcaggagaa tggcgtgaac ccaggaggcg gagcttgcag tgaaccgaga tagcctcact
     5281 gcactccagc ctgggcgaaa gagcaagact ccgtctcaaa aaaaaaaaac aaaaaaaaaa
     5341 ttagccgggc gtggtggtgg gcgcctgtag tcccagctac tcgggaggct gagacaggag
     5401 aatgatgtga acccgggagg cagagcttgc agtgagccga gattgtgcca ctgcactcca
     5461 gcctgggcga cagagcaaga ctccgtctca aaaaataaat taattaatta aattaaataa
     5521 gtgggtgttt taagtgctca gcttccttgg ttctaccacc ttctctccct gaccctcctt
     5581 ccctttcccc ctgaccttgg cctttattct agaacggatc tgaagcaata ggatataaaa
     5641 aattcacaac tgctaaaaca gtgttccttc tagtgtaatt ttcaccctta gagacctgtc
     5701 tgtaataaac attatttgaa atgcc
//
