LOCUS       NM_005006              11660 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens NADH:ubiquinone oxidoreductase core subunit S1
            (NDUFS1), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_005006
VERSION     NM_005006.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Bu,L., Zhang,L., Wang,X., Du,G., Wu,R. and Liu,W.
  TITLE     Association between NDUFS1 from urinary extracellular vesicles and
            decreased differential renal function in children with
            ureteropelvic junction obstruction
  JOURNAL   BMC Nephrol 25 (1), 158 (2024)
   PUBMED   38720274
  REMARK    GeneRIF: Association between NDUFS1 from urinary extracellular
            vesicles and decreased differential renal function in children with
            ureteropelvic junction obstruction.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11660)
  AUTHORS   Wan,S., Maitiabula,G., Wang,P., Zhang,Y., Gao,X., Zhang,L., Gao,T.
            and Wang,X.
  TITLE     Down regulation of NDUFS1 is involved in the progression of
            parenteral-nutrition-associated liver disease by increasing
            Oxidative stress
  JOURNAL   J Nutr Biochem 112, 109221 (2023)
   PUBMED   36402252
  REMARK    GeneRIF: Down regulation of NDUFS1 is involved in the progression
            of parenteral-nutrition-associated liver disease by increasing
            Oxidative stress.
REFERENCE   3  (bases 1 to 11660)
  AUTHORS   Ren,L., Meng,L., Gao,J., Lu,M., Guo,C., Li,Y., Rong,Z. and Ye,Y.
  TITLE     PHB2 promotes colorectal cancer cell proliferation and
            tumorigenesis through NDUFS1-mediated oxidative phosphorylation
  JOURNAL   Cell Death Dis 14 (1), 44 (2023)
   PUBMED   36658121
  REMARK    GeneRIF: PHB2 promotes colorectal cancer cell proliferation and
            tumorigenesis through NDUFS1-mediated oxidative phosphorylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11660)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 11660)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 11660)
  AUTHORS   Loeffen,J.L., Triepels,R.H., van den Heuvel,L.P., Schuelke,M.,
            Buskens,C.A., Smeets,R.J., Trijbels,J.M. and Smeitink,J.A.
  TITLE     cDNA of eight nuclear encoded subunits of NADH:ubiquinone
            oxidoreductase: human complex I cDNA characterization completed
  JOURNAL   Biochem Biophys Res Commun 253 (2), 415-422 (1998)
   PUBMED   9878551
REFERENCE   7  (bases 1 to 11660)
  AUTHORS   Rahman,S. and Thorburn,D.
  TITLE     Nuclear Gene-Encoded Leigh Syndrome Spectrum Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   26425749
REFERENCE   8  (bases 1 to 11660)
  AUTHORS   Duncan,A.M., Chow,W. and Robinson,B.H.
  TITLE     Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q
            reductase gene (NDUFS1) to 2q33----q34
  JOURNAL   Cytogenet Cell Genet 60 (3-4), 212-213 (1992)
   PUBMED   1505218
REFERENCE   9  (bases 1 to 11660)
  AUTHORS   Chow,W., Ragan,I. and Robinson,B.H.
  TITLE     Determination of the cDNA sequence for the human mitochondrial
            75-kDa Fe-S protein of NADH-coenzyme Q reductase
  JOURNAL   Eur J Biochem 201 (3), 547-550 (1991)
   PUBMED   1935949
REFERENCE   10 (bases 1 to 11660)
  AUTHORS   Sumegi,B. and Srere,P.A.
  TITLE     Complex I binds several mitochondrial NAD-coupled dehydrogenases
  JOURNAL   J Biol Chem 259 (24), 15040-15045 (1984)
   PUBMED   6439716
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC413278.1, BC018900.1,
            AW074155.1, CA419316.1, AA150435.1 and AC007383.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_005006.6.
            
            Summary: The protein encoded by this gene belongs to the complex I
            75 kDa subunit family. Mammalian complex I is composed of 45
            different subunits. It locates at the mitochondrial inner membrane.
            This protein has NADH dehydrogenase activity and oxidoreductase
            activity. It transfers electrons from NADH to the respiratory
            chain. The immediate electron acceptor for the enzyme is believed
            to be ubiquinone. This protein is the largest subunit of complex I
            and it is a component of the iron-sulfur (IP) fragment of the
            enzyme. It may form part of the active site crevice where NADH is
            oxidized. Mutations in this gene are associated with complex I
            deficiency. Several transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Jan 2011].
            
            Transcript Variant: This variant (1) represents the longest and
            predominant transcript. Variant 1 encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853566.16450.1,
                                           SRR1660809.44846.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000233190.11/
                                                  ENSP00000233190.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                DC413278.1         76-130
            56-2408             BC018900.1         7-2359
            2409-2748           AW074155.1         21-360              c
            2749-3373           CA419316.1         12-636              c
            3374-3398           AA150435.1         1-25                c
            3399-11660          AC007383.4         53807-62068         c
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.3"
     gene            1..11660
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="NADH:ubiquinone oxidoreductase core subunit S1"
                     /db_xref="GeneID:4719"
                     /db_xref="HGNC:HGNC:7707"
                     /db_xref="MIM:157655"
     exon            1..104
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            105..169
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..2292
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /EC_number="7.1.1.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; complex I 75kDa subunit; complex I,
                     mitochondrial respiratory chain, 75-kD subunit; NADH
                     dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa
                     (NADH-coenzyme Q reductase); mitochondrial NADH-ubiquinone
                     oxidoreductase 75 kDa subunit"
                     /codon_start=1
                     /product="NADH-ubiquinone oxidoreductase 75 kDa subunit,
                     mitochondrial isoform 1 precursor"
                     /protein_id="NP_004997.4"
                     /db_xref="CCDS:CCDS2366.1"
                     /db_xref="GeneID:4719"
                     /db_xref="HGNC:HGNC:7707"
                     /db_xref="MIM:157655"
                     /translation="MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVE
                     PGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNI
                     LTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAV
                     EDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSEL
                     SGNIIDICPVGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPR
                     MHEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQ
                     GKDVAAIAGGLVDAEALVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIA
                     GVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLKVALIGSPVDLTYTYDHLGDSPK
                     ILQDIASGSHPFSQVLKEAKKPMVVLGSSALQRNDGAAILAAVSSIAQKIRMTSGVTG
                     DWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDLPKDCFI
                     IYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIR
                     ALSEIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLAD
                     PLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAQAVEEPSIC"
     exon            170..261
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            262..369
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            370..446
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            447..528
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            529..659
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            660..845
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            846..980
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1095
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1241
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1370
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1500
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1661
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1816
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1992
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2127
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2200
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..11660
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2548..2553
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="hexamer: CATAAA"
     polyA_site      2571
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="major polyA site"
     regulatory      4455..4460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="hexamer: TATAAA"
     polyA_site      4506
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="major polyA site"
     regulatory      11639..11644
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="hexamer: AATAAA"
     polyA_site      11660
                     /gene="NDUFS1"
                     /gene_synonym="CI-75k; CI-75Kd; MC1DN5; PRO1304"
                     /note="major polyA site"
ORIGIN      
        1 agagttagcc gaggccgcca tattgaataa gcgacccggc ctcctagggg gtcgtcgtgg
       61 tccagacagt ttagcagaac agcctccgcg gctccgggga gaagcaatat gttaaggata
      121 cctgtaagaa aggccttagt aggcctttct aagtctccta aaggatgtgt tcgaacaact
      181 gccacagcag caagcaactt gattgaagta tttgttgatg gtcagtctgt catggtggaa
      241 ccgggaacga ccgtcctcca agcttgtgag aaggttggca tgcagatccc tcgattctgt
      301 tatcatgaaa ggttgtctgt tgctggaaac tgcaggatgt gccttgttga aattgagaaa
      361 gcccctaagg ttgtagctgc ttgtgccatg ccagtaatga agggttggaa tatcctaaca
      421 aactcagaaa aatccaaaaa agccagggaa ggtgtgatgg agttcttatt agcaaatcac
      481 ccattggact gtcctatttg tgaccaggga ggtgaatgtg atctgcagga ccagtccatg
      541 atgtttggaa atgataggag ccgattttta gaggggaagc gtgctgtgga agacaagaac
      601 attgggccat tggtaaagac catcatgaca agatgtatac agtgtactcg ctgcatcagg
      661 tttgcaagtg agattgcagg agtagatgat ttgggaacaa caggcagagg aaatgatatg
      721 caagttggca catacattga aaagatgttc atgtctgaac tgtctgggaa tatcattgat
      781 atctgccctg taggtgccct aacctctaag ccctatgcct ttactgcccg gccttgggaa
      841 acaagaaaga cagaatccat tgatgtaatg gatgcggttg gaagtaatat tgtggttagc
      901 acaagaactg gagaagtgat gaggattttg ccacgtatgc atgaggacat caatgaagag
      961 tggatctctg ataaaaccag atttgcctat gatgggctaa aacgtcaaag acttaccgag
     1021 ccaatggtca gaaatgaaaa agggctttta acctatactt cttgggagga tgcgctctct
     1081 cgcgtagctg gaatgttgca gagttttcaa ggcaaagatg tggcagcaat tgcaggtggc
     1141 ttggtggatg ctgaagccct ggtagctctc aaagatttgc ttaatagagt ggactctgac
     1201 accttatgca ctgaagaggt cttccccact gcaggagctg gcacagattt gcgttccaat
     1261 tatcttctta atactacaat tgctggtgtg gaagaggcag atgttgttct tctggttggt
     1321 acaaacccac gttttgaggc accactgttt aatgctagaa ttcgaaagag ctggctgcat
     1381 aatgacttaa aagtggccct tataggcagt ccagtggacc tcacttacac atatgaccac
     1441 ctgggagact cccccaaaat tcttcaagac attgcttcgg gaagccatcc atttagccag
     1501 gtcctaaagg aagctaaaaa accaatggtg gttttaggca gttctgcact ccaaagaaat
     1561 gatggagcag caattcttgc agctgtttct agcattgcac aaaagattcg gatgactagt
     1621 ggtgttactg gtgattggaa agttatgaat atccttcata ggattgcaag tcaagtagct
     1681 gctttggacc ttggctataa gcctggggtg gaagcaattc ggaagaaccc tcccaaggtg
     1741 ctgtttctcc tgggagcaga tggaggttgt atcacacgac aggatttgcc aaaggattgt
     1801 ttcattattt atcaaggaca tcatggtgat gttggggctc ccatagctga tgttattctc
     1861 ccaggagctg cttacacaga gaagtctgct acatatgtca acactgaggg tagagctcag
     1921 cagactaagg tagcagtgac acctcctggc ttggcaagag aagactggaa aattataaga
     1981 gcactctctg agattgctgg aatgactctt ccatatgata ctctggatca agtaaggaac
     2041 agattggaag aagtctctcc taatcttgtt cgatatgatg atattgaagg ggctaattac
     2101 ttccagcaag caaatgagct ctcaaagcta gtgaaccagc agcttcttgc tgacccactt
     2161 gttccacctc agctaactat aaaagacttc tacatgacag attcaattag cagagcctca
     2221 cagacaatgg ccaaatgtgt caaagctgtc acagagggtg cccaggcagt agaggaacca
     2281 tccatatgct gaagcttcta ctaggatccc agttttgccg cagataatta atggacaact
     2341 gtagtgcagt gatcctttac aggtttattt ctttgtaaaa aaaaataata ataatttgaa
     2401 tcatgtaata tttaaggtta tactatgcct atttgaaaat gatattagtt atcaactttg
     2461 cagtttgaaa aacatgtatt gtgtgtaaag gttaaataac aaaactatgc agatgctctt
     2521 aaaagcattg ataacctttg tgacgaacat aaagagatcc ttaaattatg agttgttggc
     2581 ttatcttcat aaataatttg tctgtaaaat ggatgaaatg aaaagaggtt caattaaaac
     2641 ctactttttt ctagtgctaa agaaaagatt taagcacctt gtcaagctgg gtaaatagga
     2701 aaaatacata atcatgctca gatatgtatc taggataatt ataattaata ataatcatag
     2761 taacaatggc taatgataat ttagctttat tatatgtgct aagcactctg gttttacatg
     2821 cattatcttc ttttgttctt gctacaaccc tgtgagatag tagtattatc tcattttact
     2881 gatgaagact gaagcctagg tatattaaat agcttgccca aggccacaca gcaaaagtca
     2941 ccaactcaaa cctacttctt atttactcca aagcctgttg ttcttaactg caacatatta
     3001 tttcgtctca ttaatgttga ttctataggt tgttacttct aaaaattagt attgagttta
     3061 atggtgaaca catttttcta ttttctcttg aatctgcttc tataatgtca tggtgattta
     3121 tgtggctttt ttttttttat aagttataca tgtatgcatg tatacttatg agacctccct
     3181 tggaatgagg gaggtctcaa gagatataat ttagattctc attgatgttc tgtattcatt
     3241 atcctaacac catctgtagt gttaaatcaa ctaaattatt tcagcaatag gagacaaaac
     3301 aaccagcttt cataattttt aattgtcaaa accaaaagga atcagaataa ggatcactga
     3361 gaatttaaaa aaataaaaaa ggaagtaaaa attttacaat taggagataa ttaaaaatag
     3421 ataatttttt tttttaatct aaggttgggt gtggtggctt acacctgtaa tcccaacact
     3481 tggggggcca aggtgggtgg atcgcttgag cccaggcagt tggagaccag tctgggcaac
     3541 atggtgaaac cccatctcta caaaaaatac aaaaattagc tgggtgtggt ggtgtacacc
     3601 tatagtccca gctactcgga aggctgaggt gggaggatca cctgacccag gaggttgagg
     3661 ctgcagtgag ccgtgattgc gccactgcat tccagcctgg gcaacagagc aagactttgt
     3721 ttcaaacaaa caaacaaaac tatgttcaca atttatttgt tttgtaatcc ttattctttt
     3781 ctgctttcac taaagactca gcccaagtat aggatgccct ttttcccttt gttttttttt
     3841 tttttttgag atggagtctt actctgtcac ccaaggctgg aatgcagtgg catgatcttg
     3901 gctcactgca atcaccacct ccctgattca agcgattctc ctgcctcagc ctccccggta
     3961 gctgggatta caagcgcacg ccaccacacc tggctaattt ttgtattttt agtagagaca
     4021 gggtttcacc aatttggcca ggctggtctt gaactcctaa cctcaggtga tctacctgcc
     4081 ttggcctccc aaagtgctgg gattacaggc gtgagccacc gcgcccagtc taggatgtcg
     4141 tttttctgat acaacaaagg ataaggtttt agaataatag tatgttacaa tatctttaaa
     4201 aacagcaggt gcagtggctc acacctgtaa gcccagcatt ttgggggttc aaggcaggaa
     4261 gatcagttga ggccaggagt tcaagaccag cctggactgc atagcaagat cctatttgta
     4321 caaaaaaatg taaaacttaa aattgcacaa aatttgtcac ctgtaccagc ttttagaact
     4381 gtttatctta tcctcctcag tgatacatca tgaagttgtg tgctttgcct aaaatgccca
     4441 gttacctgaa attgtataaa ttcttgccaa aagtgtttga acttaataca aacttcccat
     4501 ctcttacctc ttagcactgt gctcatcttg aggggacata gtcccaattt tgtattttat
     4561 ataatactgt taatgaatat gtgtagactt catatggttg tgggtaagag aatactgcat
     4621 tcagatagaa aagatgctat atagctaagt tgatccagga tccttgggct acctgctagg
     4681 cagcttgtgg tgaacaatca taatctctaa aaaatacctt gtctggaccg ggcgcggtgg
     4741 ctcacacctg taatcccagc actttggcag gctgaggcgg gcagatcatt gaggtcagga
     4801 gtttgagacc agcctggcca acgtggtgaa accctgtctc tactaaaaat acaaaaatta
     4861 gccaggcatg gtggcacatg gctgtaatcc cagctactgg ggaggctgaa gcaggaaaat
     4921 cgcttgaact gagagtcaag gcggaggtcg cggtaagccg agatcacacc attgcactgc
     4981 agcctgggtg aagaaacaag cctccctctc aaaaaataaa taaataaata aataatacct
     5041 cctctgggat aagtagtgct ccgtctgtct catgggcaca tattagtgtc gtaatgccaa
     5101 atactaaggt tattgtaagc ataaacattg aagataattg ctagagaaaa atagactgaa
     5161 aaagatgtaa gaaaaataca cgtagatgag agtaacctgt ctggtggaat catgacattc
     5221 taagcagtga aggaatcacc aatgagaaaa tctacaaagg ctcaagatca agtctcttta
     5281 ggtgaataag gcaatctaga aagaccttgg aataataata ccatttcctg aggaccttct
     5341 aaataagtgc cattaaagct ttaaatacat taagactatt cttcacaaaa tctctgctaa
     5401 tagtaggtat taattgagac tcaagaaata tcaaataact tatttatggc tacacagcta
     5461 gatactaatg gcaggagtgt ttaattagaa gtacttttac tatcccaggt tgcctcaaat
     5521 gaaaacattt tggacagcaa tgtaaggtga atagctgagc atgatggcac aagctatagc
     5581 cccagctact tgggaggctg aggcacaagg attgcttgag caaatgagtt gagtccagca
     5641 tggacaactg tattcctgca gcctgcttgt attcctctcc tcaaagtgct ttttccttct
     5701 ctgccacatg actagtctgt gaattttcca aacttctacg ctctgcttcc cttttaaata
     5761 tgttccaact ttaagttatt tctttgcttc catatctgat tgtaggctgc tagcatcagc
     5821 caggccacat cttgaatgct ttgctgctta gaaatttctt ctgccagata ccctgatacc
     5881 ctaagtcatc actcttaagt tcaaacttcc acagattcct agggcctgga cacaatgcac
     5941 ccaagctatt tgctaaagca tgacatagat gtacctttat tcctgttccc aataacttcc
     6001 tcatttccat ctgagacctc atcactctgg acttcaccat atgtctatca gcattttggt
     6061 cacaaccact taactagtct ctaagttcca aactttccct catctttctg tcttctgagc
     6121 cctccagatt cttccaacct ctgcctgtta cccagttcca aagctgtttc tacattttcc
     6181 aatatgtata gcagtgcccc actgtcagta ctaatctgtt ttaatctgtt tgcattactg
     6241 taaaggaata tctgagattg ggtaattctt gcaaaagaga gatttaattg gcttacagtt
     6301 tctgcaggct gtacacaaag catggcacca gcagctgctt ctgatgaggg cctcaggaag
     6361 cttacaatca tggtagaagg caaaatggga gcgagcatat cacatggtga gagagggagc
     6421 aagagagagg aggaggtccc agactctaaa caaccaaatt tcacatgaac taagatctca
     6481 ctcatcacca aggtgatggt gccaagccat tcatgaagga tttgccccat gattctatac
     6541 ctcccacgag gccctacctc cagcattggg ggttacattt caacatgaga tttggaagtg
     6601 acagccatcc aaaccatatc agcaacataa caagaccccc aactcttaaa aaaaaaagtt
     6661 gaatatatat ccaaaaatat cttgaattcc atatggaaag cctcccccct caaatttgta
     6721 gtaaattgta ttataatgaa tacattaatt aaaaatatac ttggcctggc acagtagctc
     6781 aagcctgtaa tcccagcact ttgggaggcc gaggcaggcg gatcacctga ggtcaggagt
     6841 tttcgagacc agcctgtccg acatggtgaa actctgtctc tactaaaaat acaaaattag
     6901 ccagccatgg tggcatatgc ctgtaatccc agctactcac tcaggaggct gaggcaggag
     6961 aatcacttaa acccaggaga cggaggttgc agtgagtcaa gattgcaccg ttgcactcca
     7021 gcctgggtga cagagtgaga ctctgtctga aaaaaagtaa aaattaaaaa atatatttga
     7081 caatacccag taccaattta ttgtttgttg ttgacattaa cattttcttt aagagtattt
     7141 cttcatctgt aaaatgagga taaaaattgt aaaattgtac ctacttccag cctcatagaa
     7201 ttgttttgat gattacatca gaaagcttgt aaaaacctta ggcctgtttc ctgacatata
     7261 ctaaatgccc catgtgctaa ctgaggatga tcattgtagg aaaatttcat agactatgag
     7321 ggaaagccat gcctaaaata atctccagct tattaagaag tgcattcctt tttgtttctt
     7381 tgttttatct tagattgagt ctcactgtat tgccagtctg gagtgcagtg gcgcaatctc
     7441 agctcactgc aacctccacc tcctgggttc aagcgatcct cctgcctcag cctcctgagt
     7501 agctgggact acaggagcac accaccacgc ccagctaatt tttgtgtttt tagtagaggc
     7561 ggggtttcac catgttgggc aggatggtct caaactcctg acctcaggtg atccacccgc
     7621 ctcaacctcc cagagtgctg ggattacagg cgtgagctat ccgtgcccag tcaatttttg
     7681 tatttttagt agagatgagg tttcaccata ttggtcaggc tggtcttgaa cccctgacct
     7741 caggtgattc acccgcctca gcctcccaaa gtgctgggat tccagatgtg agccagcgca
     7801 cccagcctgg ccatctttta taaaaagatg aagatgtcag aattccctaa taagattctg
     7861 gctctgttac ccagactgga gtgcaatgca tggcatgatc tcagcacact gcaacctcca
     7921 tctcctgggc tcaagccatc ctcccacttc agtctcccaa gtggctggga ctacaggcac
     7981 acgccaccac acctggctaa tttttgtatt ttttgtagag acgaggtttc gccatgttac
     8041 ccaggctggt cttaaactcc tgagttcaag tgatccacct gcctcagcct cccaaagtgc
     8101 tgggattaca ggcatgagcc actgtacccg gcccctaata agttttaaat aaccaatatt
     8161 ttaaaatatt ttaatagtta catagcactt tagtttgctg atttaattta tcccaaggga
     8221 caaggatgtt aatgagaaaa ctgactagat ttcagatcac agattttaag agaacaagga
     8281 tctcaaaacc aaataccctc tgcttaaagt gttttttgtg tttttcacta ctgaaaatgt
     8341 ttagagattg acttacctat tgctgatact caaaacatct gatatcttaa tatttttaaa
     8401 taactcacta aaagcatatg ttgaaaatgg caatgtatag tctgagactg gtgattagac
     8461 tggactaaaa gcatatgttg gaaatggcaa atatatgatc tgagactggt gattaattta
     8521 cagtattggc acctaaagag atactgagat gaataactta ttaatttcct tcttaaaatc
     8581 ataattttgg atctgtaaag gactatacaa aaaaaaattt tagtattata cctgcatttt
     8641 ataattgtgg aaatggaggc cagaaagaat aagaggcaag ttaggggcag atcttgggtt
     8701 tggatcagct cttctgatgc ctggttctgt gacattacaa agcctgaaat tcagctccaa
     8761 catctattaa acatttgcag agtatcagct gggtgcggtg gctctcataa ggccaggagt
     8821 tcaagaccag cctggccaag atagtgaaac cccatctcta ctaaaaatac aaaaattagc
     8881 cgggcatggt ggcaagacac ctgtaatccc agctactctg gaggctgagt cagagaattg
     8941 cttaaacctg ggaggcggag gttgcagtga tcccagatcg cgccactgcg ctctagcatg
     9001 ggcgacaaag cgagacccca tctcaaaaag aaaaacaaaa tttttttgca gagtatctac
     9061 tgtgtgtcaa gtacattacc caagactaca aagagaaata caatttagct aatgtcctca
     9121 agaagctaac agtatactaa aagagatatg taggcatcaa ttatagtaat gacataacat
     9181 attctaatag aggtaagtac ataacacagt gggcatgtct agttgtcttt gtttctgctg
     9241 ttaaaacaga atatcacaga ctgggtgatg ggttgattga ttgactgatt gaggcaactc
     9301 tgtcacccag gctggggttc agtggcatga tcacagttca ctgcagcctc aacctccctg
     9361 gatcaagcaa tcctcctgcc tcagcctcct gaacagctgg aactacaggt gtgagccacc
     9421 atgccccact aatttttatt tatttatttt ttttttctga gacggagtct cagtttgtcg
     9481 cccaggctga agtgcagtgg cacaatctca gctcactgca acctccacct cccgggttca
     9541 gttttcctgc ctcaatctac taggatctag ctaggattac aggtgtgcgc caccatgccc
     9601 agctaatttt tgtattttta gtagagacgg ggtttcacca tgttggtcag gctggtctca
     9661 aactcctgac ctcatgatct gcccacctcg gcctcccaaa gtgctgggat tacaggcgta
     9721 agccaccgcg cccagctaat ttttaaaaat attttataga gacaggtctc actatgttgc
     9781 ccaggctcgt cttgaacccc tgggcccagg cgctcctccc accttgacct cccaaagttc
     9841 tggaattaca catgtgagcc aacacaccag gcccagactg ggtaatttat aaagaaattt
     9901 acttctgccc acaccgcccc gcctccccgc gcgagtggct gaggcgtagc gctgccatcc
     9961 ccgcacccct gggaacatgg cgctgcgagt ggtgcggagc gtgcgggccg tgctctgcag
    10021 cctgcacgtg gtcctggcac ctgccgcgcc ctgcctatcg aggccctggc agcttgggat
    10081 gggcgccgtc tggttactgt aatctgcctg aagttgggac aaaattgaac aaacaagatg
    10141 agtttggtgc tttggagagt gtgaaagctg ctagtgaact ctattctcct ttatcaggag
    10201 aagtaaccga aattaatgaa gctcttgcag aaaatccagg acttgtaaac aaatctcgtt
    10261 atgaagatgg ttggctgatc aagatgacac tgagtaaccc ttcataacta gatgaactta
    10321 tgagtgaaga agcatatgag aaatacataa aatctattga ggagtgaaaa tggaaccact
    10381 aaataagcta gtatgaaata acgcaaccca gcagagttgt cctaaattag tggtggatag
    10441 aagacttaga atagcaactt ttagcattac ccatgggaaa aaaaaactac tgttaacact
    10501 gctaatgaaa gaagatgcct tttaactttc taatgattgt agataaacat aatatgtgtc
    10561 tttttcacaa catcctatga tttttagact aggctctagt attcatgaaa ttatccatga
    10621 aattatccat ggtaaaaatt agttacaaaa attacataat tcaaagataa tgttgttatt
    10681 cttaagcctt atataatatt gtaacttgtg tatgtccata cctggacttg ggatgaaata
    10741 cttaatgatc tttccattgg aaataactgg gagtgaagaa gtttttgttg cttgtacagt
    10801 gtcagatgaa gaacaacact atcttaattt tgcaatacac tgcatttgct ggtgctgttt
    10861 ttatatagtg aagcaacagc tgtacagcaa aataataaaa tactcacttc ttcgttaaaa
    10921 aaaaaaaaaa tttacttctt acaattctgg aggccaggaa gaccatgatc aggtgccagc
    10981 atctgggaag ggccttcttg ctgtcctccc atggcagaag atggaagggc aagggagagc
    11041 taacatgctc ccgcaaaccc tttttataat ggcatcaatc aaatatgagg ccagagtcct
    11101 tgtgacctaa tcatctccca gaaggctccg cctcccaacc ctgttgcatt gggattaagt
    11161 ttccaacaca tgaattgtgg agacaacaca ttcaaaacat agcattccac accttgggct
    11221 ccccagattc atgtcctcac atgcaaaata aattcattcc atcccaatag cccctaaaaa
    11281 gtcttaactt gttccagcat caactttaaa gtcaaagtcc aaagtctcat ctaaatcaga
    11341 tatgagtgag actcaaggca tgattcatca tgagacaaag gatgtacatt tgcaatgttt
    11401 gtcatgtcag acaaaacaaa aatatgtaaa tatccatcaa tagggaactg ctgaaaaatt
    11461 tttttgtata atcataaaat gaaacatgca gatgtttaaa ccaatgagct agatctcaac
    11521 gtgctgatat ggaaagtgct tcagaatgta ttaaggacat aaattaagtg tacaataatg
    11581 tgtgtgtgtg tatatatgta tatgcttacg tgtgtatgga aagtatctca gcagatacaa
    11641 taaaaactta attgtgatta
//
