LOCUS       NM_001203260            2079 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens NDUFC2-KCTD14 readthrough (NDUFC2-KCTD14), transcript
            variant 1, mRNA.
ACCESSION   NM_001203260
VERSION     NM_001203260.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Prakash,T., Sharma,V.K., Adati,N., Ozawa,R., Kumar,N., Nishida,Y.,
            Fujikake,T., Takeda,T. and Taylor,T.D.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP003032.2 and FY213449.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001203260.1.
            
            Summary: This locus represents naturally occurring read-through
            transcription between the neighboring NDUFC2 (NADH dehydrogenase
            (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa) and KCTD14
            (potassium channel tetramerisation domain containing 14) genes on
            chromosome 11. The read-through transcripts share sequence identity
            with the upstream gene product and one variant has a frameshifted
            C-terminal region derived from the downstream gene exons. [provided
            by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.34322.1, FY211644.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146411, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 4718, 65987
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-256               AP003032.2         87265-87520         c
            257-619             FY213449.1         168-530
            620-2079            AP003032.2         23373-24832         c
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q14.1"
     gene            1..2079
                     /gene="NDUFC2-KCTD14"
                     /note="NDUFC2-KCTD14 readthrough"
                     /db_xref="GeneID:100532726"
                     /db_xref="HGNC:HGNC:42956"
     exon            1..284
                     /gene="NDUFC2-KCTD14"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..523
                     /gene="NDUFC2-KCTD14"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NDUFC2-KCTD14 readthrough transcript protein; NADH
                     dehydrogenase [ubiquinone] 1 subunit C2"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 subunit C2,
                     isoform 2 isoform 1"
                     /protein_id="NP_001190189.1"
                     /db_xref="GeneID:100532726"
                     /db_xref="HGNC:HGNC:42956"
                     /translation="MIARRNPEPLRFLPDEARSLPPPKLTDPRLLYIGFLGYCSGLID
                     NLIRRRPIATAGLHRQLLYITAFFFAGYYLVKREDYLYAVRDREMFGYMKLHPEDFPE
                     EGGDIGLCCLSPGSLSDTPAVFAVGSSAATTH"
     exon            285..428
                     /gene="NDUFC2-KCTD14"
                     /inference="alignment:Splign:2.1.0"
     exon            429..523
                     /gene="NDUFC2-KCTD14"
                     /inference="alignment:Splign:2.1.0"
     exon            524..2079
                     /gene="NDUFC2-KCTD14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2060..2065
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFC2-KCTD14"
                     /note="hexamer: AATAAA"
     polyA_site      2079
                     /gene="NDUFC2-KCTD14"
                     /note="major polyA site"
ORIGIN      
        1 gagtccggcg cgcagaggag gaggagaaag ctgaccgctt aggccggggt agtggtcgtc
       61 gtggttttcc ttgtagttcg tggtctgaga ccaggcctca agtggaaacg gcgtcaccat
      121 gatcgcacgg cggaacccag aacccttacg gtttctgccg gatgaggccc ggagcctgcc
      181 cccgcccaag ctgaccgacc cgcggctcct ctacatcggc ttcttgggct actgctccgg
      241 cctgattgat aacctaatcc ggcggaggcc gatcgcgacg gctggtttgc atcgccagct
      301 tctatatatt acggcctttt tttttgctgg atattatctt gtaaaacgtg aagactacct
      361 gtatgctgtg agggaccgtg aaatgtttgg atatatgaaa ttacatccag aggattttcc
      421 tgaagaaggg ggtgacattg gcctctgttg cttgtcccca ggatccctct ctgacacccc
      481 tgctgtgttt gctgtgggga gttctgctgc caccacccat tagatgtcta ctgttgtgga
      541 gctgaacgtc gggggtgagt tccacaccac caccctgggt accctgagga agtttccggg
      601 ctcaaagctg gcagagatgt tctctagctt agccaaggcc tccacggacg cggagggccg
      661 cttcttcatc gaccgcccca gcacctattt cagacccatc ctggactacc tgcgcactgg
      721 gcaagtgccc acacagcaca tccctgaagt gtaccgtgag gctcagttct acgaaatcaa
      781 gcctttggtc aagctgctgg aggacatgcc acagatcttt ggtgagcagg tgtctcggaa
      841 gcagtttttg ctgcaagtgc cgggctacag cgagaacctg gagctcatgg tgcgcctggc
      901 acgtgcagaa gccataacag cacggaagtc cagcgtgctt gtgtgcctgg tggaaactga
      961 ggagcaggat gcatattatt cagaggtcct gtgttttctg caggataaga agatgttcaa
     1021 gtctgttgtc aagtttgggc cctggaaggc ggtcctagac aacagcgacc tcatgcactg
     1081 cctggagatg gacattaagg cccaggggta caaggtattc tccaagttct acctgacgta
     1141 ccccaccaaa agaaacgaat tccattttaa catttattca ttcaccttca cctggtggtg
     1201 atcctcagga gcagagactg ttatgaattc tggcgtggct tatgaaatta aaagttgcca
     1261 tcaaagccat tttcttttaa tttcacaaac atcaggcaat ttccagggtt ggtctagagt
     1321 cttgccacta aatattgatc actcgtttaa ggactttcca ctccattgca actgatgcca
     1381 ctatatttgc ctagcaactt gcagctactt ccttttcaaa gcctcatgta tctcccagac
     1441 ccttctcttg aagtccaata acaagaccaa gtaagaatgt ttcaacaatg cgttggcaag
     1501 agatgtgaga tgacaacagg aacatacaag atactgtgaa tctagatgtt ctgacctaaa
     1561 gatgtagtct acatagcccc agcttggggt ccaatccatc tgtccctggc atgtgccttc
     1621 atgtagtagg tgctttcctg atcccctttg cgagatgctg tgggtgctaa cacctcagag
     1681 ctgtcctctt ctctagagtg gaggttttca aagtgcatca tcagcattac ctgtgaactt
     1741 gctggaaata caaatcctca ggccccacct cagacctact gaatcagaat ctctgggggt
     1801 tggcacagca ttctgattta ccaaaccctc caagtgattt tgatgtattc taattttgag
     1861 accatctcta gaaaagaatt gctacctctt gtatggaggt acaaaagact gacctcttac
     1921 atcaaggaac ttcctttccc agagctcctc atggaatcaa gctgaagtca gtcttcttct
     1981 gagagcacat tcttactcag tttttttcct ctgtcctacg ctgcttccct cactcccctt
     2041 ctcctaagag cactccatca ataaaccact tgcacgaga
//
