LOCUS       NM_004548                675 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10),
            mRNA.
ACCESSION   NM_004548
VERSION     NM_004548.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Arroum,T., Borowski,M.T., Marx,N., Schmelter,F., Scholz,M.,
            Psathaki,O.E., Hippler,M., Enriquez,J.A. and Busch,K.B.
  TITLE     Loss of respiratory complex I subunit NDUFB10 affects complex I
            assembly and supercomplex formation
  JOURNAL   Biol Chem 404 (5), 399-415 (2023)
   PUBMED   36952351
  REMARK    GeneRIF: Loss of respiratory complex I subunit NDUFB10 affects
            complex I assembly and supercomplex formation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 675)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 675)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 675)
  AUTHORS   Signes,A. and Fernandez-Vizarra,E.
  TITLE     Assembly of mammalian oxidative phosphorylation complexes I-V and
            supercomplexes
  JOURNAL   Essays Biochem 62 (3), 255-270 (2018)
   PUBMED   30030361
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 675)
  AUTHORS   Yano,M., Terada,K. and Mori,M.
  TITLE     AIP is a mitochondrial import mediator that binds to both import
            receptor Tom20 and preproteins
  JOURNAL   J Cell Biol 163 (1), 45-56 (2003)
   PUBMED   14557246
REFERENCE   7  (bases 1 to 675)
  AUTHORS   Murray,J., Zhang,B., Taylor,S.W., Oglesbee,D., Fahy,E.,
            Marusich,M.F., Ghosh,S.S. and Capaldi,R.A.
  TITLE     The subunit composition of the human NADH dehydrogenase obtained by
            rapid one-step immunopurification
  JOURNAL   J Biol Chem 278 (16), 13619-13622 (2003)
   PUBMED   12611891
REFERENCE   8  (bases 1 to 675)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
REFERENCE   9  (bases 1 to 675)
  AUTHORS   Loeffen,J.L., Triepels,R.H., van den Heuvel,L.P., Schuelke,M.,
            Buskens,C.A., Smeets,R.J., Trijbels,J.M. and Smeitink,J.A.
  TITLE     cDNA of eight nuclear encoded subunits of NADH:ubiquinone
            oxidoreductase: human complex I cDNA characterization completed
  JOURNAL   Biochem Biophys Res Commun 253 (2), 415-422 (1998)
   PUBMED   9878551
REFERENCE   10 (bases 1 to 675)
  AUTHORS   Emahazion,T., Beskow,A., Gyllensten,U. and Brookes,A.J.
  TITLE     Intron based radiation hybrid mapping of 15 complex I genes of the
            human electron transport chain
  JOURNAL   Cytogenet Cell Genet 82 (1-2), 115-119 (1998)
   PUBMED   9763677
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP319067.1 and BC005829.2.
            
            On Nov 22, 2018 this sequence version replaced NM_004548.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF088991.1, SRR1163655.207088.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000268668.11/
                                                  ENSP00000268668.6
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                BP319067.1         31-50
            21-675              BC005829.2         1-655
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..675
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /note="NADH:ubiquinone oxidoreductase subunit B10"
                     /db_xref="GeneID:4716"
                     /db_xref="HGNC:HGNC:7696"
                     /db_xref="MIM:603843"
     exon            1..217
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..606
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /EC_number="7.1.1.2"
                     /note="complex I PDSW subunit; NADH ubiquinone
                     oxidoreductase PDSW subunit (RH 16p13.3); CI-PDSW; complex
                     I-PDSW; NADH-ubiquinone oxidoreductase PDSW subunit; NADH
                     dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 beta
                     subcomplex subunit 10"
                     /protein_id="NP_004539.1"
                     /db_xref="CCDS:CCDS10451.1"
                     /db_xref="GeneID:4716"
                     /db_xref="HGNC:HGNC:7696"
                     /db_xref="MIM:603843"
                     /translation="MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVRE
                     FIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQD
                     RLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQE
                     RKAAKEAAAATS"
     exon            218..356
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /inference="alignment:Splign:2.1.0"
     exon            357..496
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /inference="alignment:Splign:2.1.0"
     exon            497..675
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /inference="alignment:Splign:2.1.0"
     regulatory      657..662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /note="hexamer: TATAAA"
     polyA_site      675
                     /gene="NDUFB10"
                     /gene_synonym="MC1DN35; PDSW"
                     /note="major polyA site"
ORIGIN      
        1 gtagcgggcg acctaggccg cgggacccgg acggaggtag aggccagggc agcgcgtccg
       61 ggagcggagt ccgcgcccgc cgccgccatg ccggacagct gggacaagga tgtgtaccct
      121 gagcccccgc gccgcacgcc ggtgcagccc aatcccatcg tctacatgat gaaagcgttc
      181 gacctcatcg tggaccgacc cgtgaccctc gtgagagaat ttatagagcg gcagcacgca
      241 aagaacaggt attactacta ccaccggcag taccgccgcg tgccagacat cactgagtgc
      301 aaggaggagg acatcatgtg catgtatgaa gccgaaatgc agtggaagag ggactacaaa
      361 gtcgaccaag aaattatcaa cattatgcag gatcggctca aagcctgtca gcagagggaa
      421 ggacagaact accagcagaa ctgtatcaag gaagtggagc agttcaccca ggtggccaag
      481 gcctaccagg accgctatca ggacctgggg gcctacagtt ctgccaggaa gtgcctggcc
      541 aaacagaggc agaggatgct gcaagagaga aaagctgcaa aagaggccgc cgctgccacc
      601 tcctgaggca gctgtgggtg cccctgctgt gtggctctgt atgactgttg ctgaaatata
      661 aagccctgca acctg
//
