LOCUS       NM_004545                317 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens NADH:ubiquinone oxidoreductase subunit B1 (NDUFB1),
            mRNA.
ACCESSION   NM_004545
VERSION     NM_004545.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 317)
  AUTHORS   Signes,A. and Fernandez-Vizarra,E.
  TITLE     Assembly of mammalian oxidative phosphorylation complexes I-V and
            supercomplexes
  JOURNAL   Essays Biochem 62 (3), 255-270 (2018)
   PUBMED   30030361
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 317)
  AUTHORS   Guo,R., Zong,S., Wu,M., Gu,J. and Yang,M.
  TITLE     Architecture of Human Mitochondrial Respiratory Megacomplex
            I2III2IV2
  JOURNAL   Cell 170 (6), 1247-1257 (2017)
   PUBMED   28844695
REFERENCE   4  (bases 1 to 317)
  AUTHORS   Stroud,D.A., Surgenor,E.E., Formosa,L.E., Reljic,B., Frazier,A.E.,
            Dibley,M.G., Osellame,L.D., Stait,T., Beilharz,T.H., Thorburn,D.R.,
            Salim,A. and Ryan,M.T.
  TITLE     Accessory subunits are integral for assembly and function of human
            mitochondrial complex I
  JOURNAL   Nature 538 (7623), 123-126 (2016)
   PUBMED   27626371
REFERENCE   5  (bases 1 to 317)
  AUTHORS   Murray,J., Zhang,B., Taylor,S.W., Oglesbee,D., Fahy,E.,
            Marusich,M.F., Ghosh,S.S. and Capaldi,R.A.
  TITLE     The subunit composition of the human NADH dehydrogenase obtained by
            rapid one-step immunopurification
  JOURNAL   J Biol Chem 278 (16), 13619-13622 (2003)
   PUBMED   12611891
REFERENCE   6  (bases 1 to 317)
  AUTHORS   Loeffen,J.L., Triepels,R.H., van den Heuvel,L.P., Schuelke,M.,
            Buskens,C.A., Smeets,R.J., Trijbels,J.M. and Smeitink,J.A.
  TITLE     cDNA of eight nuclear encoded subunits of NADH:ubiquinone
            oxidoreductase: human complex I cDNA characterization completed
  JOURNAL   Biochem Biophys Res Commun 253 (2), 415-422 (1998)
   PUBMED   9878551
REFERENCE   7  (bases 1 to 317)
  AUTHORS   Mao,M., Fu,G., Wu,J.S., Zhang,Q.H., Zhou,J., Kan,L.X., Huang,Q.H.,
            He,K.L., Gu,B.W., Han,Z.G., Shen,Y., Gu,J., Yu,Y.P., Xu,S.H.,
            Wang,Y.X., Chen,S.J. and Chen,Z.
  TITLE     Identification of genes expressed in human CD34(+) hematopoietic
            stem/progenitor cells by expressed sequence tags and efficient
            full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 95 (14), 8175-8180 (1998)
   PUBMED   9653160
REFERENCE   8  (bases 1 to 317)
  AUTHORS   Emahazion,T., Beskow,A., Gyllensten,U. and Brookes,A.J.
  TITLE     Intron based radiation hybrid mapping of 15 complex I genes of the
            human electron transport chain
  JOURNAL   Cytogenet Cell Genet 82 (1-2), 115-119 (1998)
   PUBMED   9763677
REFERENCE   9  (bases 1 to 317)
  AUTHORS   Ton,C., Hwang,D.M., Dempsey,A.A. and Liew,C.C.
  TITLE     Identification and primary structure of five human NADH-ubiquinone
            oxidoreductase subunits
  JOURNAL   Biochem Biophys Res Commun 241 (2), 589-594 (1997)
   PUBMED   9425316
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM052981.1 and BX476462.1.
            
            On Oct 20, 2018 this sequence version replaced NM_004545.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU960614.1, CB106658.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2153427
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000605997.6/
                                                  ENSP00000475170.1
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-310               BM052981.1         104-413
            311-317             BX476462.1         313-319
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12"
     gene            1..317
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /note="NADH:ubiquinone oxidoreductase subunit B1"
                     /db_xref="GeneID:4707"
                     /db_xref="HGNC:HGNC:7695"
                     /db_xref="MIM:603837"
     exon            1..65
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /inference="alignment:Splign:2.1.0"
     exon            66..210
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..247
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /note="complex I MNLL subunit; complex I-MNLL;
                     NADH-ubiquinone oxidoreductase MNLL subunit; NADH
                     dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 beta
                     subcomplex subunit 1"
                     /protein_id="NP_004536.3"
                     /db_xref="CCDS:CCDS9901.2"
                     /db_xref="GeneID:4707"
                     /db_xref="HGNC:HGNC:7695"
                     /db_xref="MIM:603837"
                     /translation="MVNLLQIVRDHWVHVLVPMGFVIGCYLDRKSDERLTAFRNKSML
                     FKRELQPSEEVTWK"
     exon            211..317
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      294..299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /note="hexamer: AATAAA"
     polyA_site      317
                     /gene="NDUFB1"
                     /gene_synonym="CI-MNLL; CI-SGDH; MNLL"
                     /note="major polyA site"
ORIGIN      
        1 actggcgcgg gttgagttcc ctgttgccct tggtctcggg gtcgctgtag gcgctgaggc
       61 tgcagctatc atggtgaact tacttcagat tgtgcgggac cactgggttc atgttcttgt
      121 ccctatggga tttgtcattg gatgttattt agacagaaag agtgatgaac ggctaactgc
      181 cttccggaac aagagtatgt tatttaaaag ggaattgcaa cccagtgaag aagttacctg
      241 gaagtaaaga ctggctagat tatcgaatgt tcacatttta aagttctgag agaaataaaa
      301 acatgaagaa tctgaaa
//
