LOCUS       NM_024120               5449 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens NADH:ubiquinone oxidoreductase complex assembly factor
            5 (NDUFAF5), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_024120
VERSION     NM_024120.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Brabbing-Goldstein,D., Kozlova,D., Bazak,L., Basel-Salmon,L.,
            Gilboa,Y., Marciano-Levi,I., Zahra,J., Kanengisser-Pines,B.,
            Botvinik,A., Kurolap,A., Birnbaum,R. and Yaron,Y.
  TITLE     Unique prenatal manifestations of biallelic NDUFAF5 variants:
            expansion of phenotype
  JOURNAL   Ultrasound Obstet Gynecol 63 (3), 392-398 (2024)
   PUBMED   37718619
  REMARK    GeneRIF: Unique prenatal manifestations of biallelic NDUFAF5
            variants: expansion of phenotype.
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Chen,P.S., Lee,N.C., Sung,C.J., Liu,Y.W., Weng,W.C., Fan,P.C.,
            Lee,W.T., Chien,Y.H., Wu,C.S., Sung,Y.F., Tsai,M.C., Lee,Y.C.,
            Hsueh,H.W., Fan,S.M., Wu,M.C., Li,H., Chen,H.Y., Lin,H.I.,
            Ou-Yang,C.H., Hwuh,W.L. and Lin,C.H.
  TITLE     Phenotypic Heterogeneity in Patients with Mutations in the
            Mitochondrial Complex I Assembly Gene NDUFAF5
  JOURNAL   Mov Disord 38 (12), 2217-2229 (2023)
   PUBMED   37752895
  REMARK    GeneRIF: Phenotypic Heterogeneity in Patients with Mutations in the
            Mitochondrial Complex I Assembly Gene NDUFAF5.
REFERENCE   3  (bases 1 to 5449)
  AUTHORS   Liu,H.H., Lee,C.H., Hsieh,Y.C., Zheng,J.H., Liu,Y.R., Chang,C.H.
            and Cho,E.C.
  TITLE     Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated Cancer
            Cell Migration and the Proliferation of Colon Cancer In Vitro and
            In Vivo
  JOURNAL   Genes (Basel) 13 (11), 2111 (2022)
   PUBMED   36421786
  REMARK    GeneRIF: Mitochondrial Factor C20orf7 Facilitates the EMT-Mediated
            Cancer Cell Migration and the Proliferation of Colon Cancer In
            Vitro and In Vivo.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5449)
  AUTHORS   Rensvold,J.W., Shishkova,E., Sverchkov,Y., Miller,I.J.,
            Cetinkaya,A., Pyle,A., Manicki,M., Brademan,D.R., Alanay,Y.,
            Raiman,J., Jochem,A., Hutchins,P.D., Peters,S.R., Linke,V.,
            Overmyer,K.A., Salome,A.Z., Hebert,A.S., Vincent,C.E.,
            Kwiecien,N.W., Rush,M.J.P., Westphall,M.S., Craven,M., Akarsu,N.A.,
            Taylor,R.W., Coon,J.J. and Pagliarini,D.J.
  TITLE     Defining mitochondrial protein functions through deep multiomic
            profiling
  JOURNAL   Nature 606 (7913), 382-388 (2022)
   PUBMED   35614220
REFERENCE   5  (bases 1 to 5449)
  AUTHORS   Rhein,V.F., Carroll,J., Ding,S., Fearnley,I.M. and Walker,J.E.
  TITLE     NDUFAF5 Hydroxylates NDUFS7 at an Early Stage in the Assembly of
            Human Complex I
  JOURNAL   J Biol Chem 291 (28), 14851-14860 (2016)
   PUBMED   27226634
  REMARK    GeneRIF: However, similar to another family member, RdmB, it
            catalyzes the introduction of a hydroxyl group, in the case of
            NDUFAF5, into Arg-73 in the NDUFS7 subunit of human complex I
REFERENCE   6  (bases 1 to 5449)
  AUTHORS   Saada,A., Edvardson,S., Shaag,A., Chung,W.K., Segel,R., Miller,C.,
            Jalas,C. and Elpeleg,O.
  TITLE     Combined OXPHOS complex I and IV defect, due to mutated complex I
            assembly factor C20ORF7
  JOURNAL   J Inherit Metab Dis 35 (1), 125-131 (2012)
   PUBMED   21607760
  REMARK    GeneRIF: analysis of the combined OXPHOS complex I and IV defect,
            due to mutated complex I assembly factor C20ORF7
REFERENCE   7  (bases 1 to 5449)
  AUTHORS   Gerards,M., Sluiter,W., van den Bosch,B.J., de Wit,L.E.,
            Calis,C.M., Frentzen,M., Akbari,H., Schoonderwoerd,K.,
            Scholte,H.R., Jongbloed,R.J., Hendrickx,A.T., de Coo,I.F. and
            Smeets,H.J.
  TITLE     Defective complex I assembly due to C20orf7 mutations as a new
            cause of Leigh syndrome
  JOURNAL   J Med Genet 47 (8), 507-512 (2010)
   PUBMED   19542079
  REMARK    GeneRIF: A new cause of Leigh syndrome can be a defect in early
            complex I assembly due to C20orf7 mutations.
REFERENCE   8  (bases 1 to 5449)
  AUTHORS   Sugiana,C., Pagliarini,D.J., McKenzie,M., Kirby,D.M., Salemi,R.,
            Abu-Amero,K.K., Dahl,H.H., Hutchison,W.M., Vascotto,K.A.,
            Smith,S.M., Newbold,R.F., Christodoulou,J., Calvo,S., Mootha,V.K.,
            Ryan,M.T. and Thorburn,D.R.
  TITLE     Mutation of C20orf7 disrupts complex I assembly and causes lethal
            neonatal mitochondrial disease
  JOURNAL   Am J Hum Genet 83 (4), 468-478 (2008)
   PUBMED   18940309
  REMARK    GeneRIF: C20orf7 is crucial in the assembly of complex I and
            mutations in C20orf7 cause mitochondrial disease
REFERENCE   9  (bases 1 to 5449)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   10 (bases 1 to 5449)
  AUTHORS   Rahman,S. and Thorburn,D.
  TITLE     Nuclear Gene-Encoded Leigh Syndrome Spectrum Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   26425749
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA289766.1, BC005984.1,
            AL109657.8 and AK025977.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024120.4.
            
            Summary: The NADH-ubiquinone oxidoreductase complex (complex I) of
            the mitochondrial respiratory chain catalyzes the transfer of
            electrons from NADH to ubiquinone, and consists of at least 43
            subunits. The complex is located in the inner mitochondrial
            membrane. This gene encodes a mitochondrial protein that is
            associated with the matrix face of the mitochondrial inner membrane
            and is required for complex I assembly. A mutation in this gene
            results in mitochondrial complex I deficiency. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.280744.1,
                                           SRR1660807.134291.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 18940309
            MANE Ensembl match                 :: ENST00000378106.10/
                                                  ENSP00000367346.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               DA289766.1         3-190
            189-1093            BC005984.1         1-905
            1094-1999           AL109657.8         25876-26781
            2000-2290           AK025977.1         1406-1696
            2291-5449           AL109657.8         27073-30231
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.1"
     gene            1..5449
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="NADH:ubiquinone oxidoreductase complex assembly
                     factor 5"
                     /db_xref="GeneID:79133"
                     /db_xref="HGNC:HGNC:15899"
                     /db_xref="MIM:612360"
     exon            1..263
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1079
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable methyltransferase C20orf7, mitochondrial; NADH
                     dehydrogenase [ubiquinone] 1 alpha subcomplex assembly
                     factor 5; NADH dehydrogenase (ubiquinone) complex I,
                     assembly factor 5; putative methyltransferase NDUFAF5;
                     arginine-hydroxylase NDUFAF5, mitochondrial"
                     /codon_start=1
                     /product="arginine-hydroxylase NDUFAF5, mitochondrial
                     isoform 1"
                     /protein_id="NP_077025.2"
                     /db_xref="CCDS:CCDS13118.1"
                     /db_xref="GeneID:79133"
                     /db_xref="HGNC:HGNC:15899"
                     /db_xref="MIM:612360"
                     /translation="MLRPAGLWRLCRRPWAARVPAENLGRREVTSGVSPRGSTSPRTL
                     NIFDRDLKRKQKNWAARQPEPTKFDYLKEEVGSRIADRVYDIPRNFPLALDLGCGRGY
                     IAQYLNKETIGKFFQADIAENALKNSSETEIPTVSVLADEEFLPFKENTFDLVVSSLS
                     LHWVNDLPRALEQIHYILKPDGVFIGAMFGGDTLYELRCSLQLAETEREGGFSPHISP
                     FTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMEDLQGMGESNCAWNRKALLHR
                     DTMLAAAAVYREMYRNEDGSVPATYQIYYMIGWKYHESQARPAERGSATVSFGELGKI
                     NNLMPPGKKSQ"
     exon            264..304
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            305..368
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            369..416
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            417..520
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            521..560
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            561..758
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            759..819
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            820..903
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            904..986
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     exon            987..5449
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1075..1080
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="hexamer: AATAAA"
     polyA_site      1097
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="major polyA site"
     regulatory      2271..2276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="hexamer: AGTAAA"
     polyA_site      2290
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
     regulatory      4620..4625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="hexamer: AATAAA"
     polyA_site      4639
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
     regulatory      5430..5435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
                     /note="hexamer: GATAAA"
     polyA_site      5449
                     /gene="NDUFAF5"
                     /gene_synonym="bA526K24.2; C20orf7; dJ842G6.1; MC1DN16"
ORIGIN      
        1 atgcgcacaa aaagcgccgg caattggggt cgcagctgga gatgctgcgg ccggcagggc
       61 tctggcgctt atgtcggcga ccttgggcgg cgagggtccc agcggagaat cttggccgta
      121 gggaagtcac ctctggtgtc tctccccgcg gtagcacctc gcccagaacc ctgaatattt
      181 tcgaccggga tttgaaaagg aaacagaaga actgggcagc ccggcagccc gagccgacca
      241 aatttgacta cctgaaggag gaggttggaa gtcggatcgc agaccgtgta tatgacatac
      301 ccagaaattt cccccttgct ttggatcttg gttgtggaag aggttacatt gcacaatatt
      361 tgaataagga aactattgga aagtttttcc aagctgacat tgcagaaaat gctttgaaaa
      421 attcctcaga aacagaaata cctactgtca gcgttttagc tgatgaagaa ttccttccct
      481 tcaaagaaaa tacatttgac ctggtggtta gcagtttaag tttgcattgg gtgaatgacc
      541 ttcctagagc acttgagcag attcattata ttttaaaacc agatggagtg tttatcggtg
      601 caatgtttgg aggcgacaca ctctatgaac ttcggtgttc cttacagtta gcggaaacgg
      661 aaagggaagg aggattttct ccacacattt ctcctttcac tgctgtcaat gacctgggac
      721 atctgcttgg gagagctggc tttaatactc tgactgtgga cactgatgaa attcaagtta
      781 actatcctgg aatgtttgaa ttgatggaag atttacaagg tatgggtgag agtaactgtg
      841 cttggaatag aaaagccctg ctgcatcgag acacaatgct ggcagctgcg gcagtgtaca
      901 gagaaatgta cagaaatgaa gatggttcag tacctgctac ataccagatc tattacatga
      961 taggatggaa atatcatgag tcacaggcaa gaccagctga aagaggttcc gcaactgtgt
     1021 catttggaga gctaggaaaa ataaacaacc ttatgccacc ggggaaaaaa tcacaataaa
     1081 tatttattca gtgttaatgt cgtccagaat tttcatcaga aatggatagc tttaacatct
     1141 aaaattatta tattttgaag caagaagcac tctaagctat ttactaatag gcttttcata
     1201 taattaggaa aacctaatat cacatctata gtaaccattt cagtttcata ttgtttctgt
     1261 tctcataagg atactgctga gtgtctttgc agattcagcc taaaagcaaa gaaaatattt
     1321 ccctaaaata tttgcaaata atgttcacat atgtaaatgc cttggaaaat attactcatg
     1381 ctgacctttt acaccttttt catttgtcat actgttttct ttgccttgaa gaggaacaga
     1441 tgaatatgca ccttctccag agttatgtgg ttttagaatt tagaagagca tgtatcttta
     1501 ttaaatccct cctcaactct ttttttttaa agcatctgaa tttaacctta tttctttttt
     1561 atctccatgg tgtgggtggg accaccatgg ttttacaccc caccctacct tagggaagaa
     1621 gaaaacattt taaagaatac cagaagtaga atcacatgta acagtctctg cacagtcatc
     1681 agtttattgt taagctttcc tttgtaattt cagggcttaa gcactattat cctaatccaa
     1741 gttcacagtc ctgttgttgt cgaggagggt tcaaaatcat ttggagataa tcaagctcag
     1801 acagcttagg ataggtgaga agaacataga ggaagcaggg agaaggctga gaagcaagca
     1861 ggagtgagcg ctaagtgttt tgtttccagt tagctgttcg ctgtcttttc catttaacct
     1921 gggcactgcc ctagcctcat cttcagcctt cagtgatcta taatagcatt tccttctgtc
     1981 tcctctcagt ctctcttctg ccttccttcc ctcctttact gtattagcaa caagctgtcc
     2041 atgggtgggg gctgtgtgtt agcctgtacg tagcacatgg gactttccac atagtagata
     2101 ttcagttaca ttttgaacta attatataac aaacttgccc tttgaaagac taagttatag
     2161 ttaaaaacca agatttgtag gagaaaaaga aattattgtt cccttcagtt tgaaaaatca
     2221 tcgatatttg aaactgggat acgcttggta gcttttttgt tttaacacaa agtaaacctg
     2281 tgaagtagaa tttggcgttt tgttttttgg ggtttttttt ttttttagct taattttcag
     2341 aacttgaaga gagagaacaa caacaaaaaa caaactgcgc tagccaggtg caatggcgca
     2401 tgcctgtagt cccagctact tgggaggctg aggcaggcag gagttcaagg ctgcagtgag
     2461 ctgtctgtgc cacggcactc caacctgggc aaaagaggag acctcatcta aagaaaaatc
     2521 caccaaaaac cctgtgtttt aaagtgcaaa ctgatgatgg aggaatgggt aaattccagg
     2581 ggtctatatt tccaaaagga catgtgtttt tgtgcactaa tacttgtttt tgaagaacac
     2641 atctcagtgt taagtggctg ttgctgagga aggggactga ggaactgggt gtgtctgtgt
     2701 cattctgttt gaatgtctgt caggggtata gaaactagga gggagacttg tacttttcac
     2761 tttttaaact tcctgtaaaa tttgaatttt ccaagcaaga atattacttt tgcttattta
     2821 atgtcaacgg aattatatgt ggatggtggg gtggagctaa ggattactct actctggcta
     2881 tcccaaaaaa agctccattt gtttaaaaaa attaagtaaa tcaaacacag ctaattgagg
     2941 acccttattg tttaatgtga gtgattgtag tgaaaacact tattttagtg tgtataacac
     3001 tagtgaaatt atttttgtag ttaaatctgc tggaagtgtg ctattaagct tttattgttt
     3061 ggagccctca ggaaactaat ttttctgaat aaccacattt tccaaatgat taagagctca
     3121 tccttttaag caatgaaact tttaaaaggt ctttatcaaa aatttttcat aaatgttaca
     3181 ttcttaaatg gtatatgata aataatcatc ttttttgttg gtttattttt ttatttttta
     3241 ttttatttta ttttttgaga cggagtctcg ctctgtctcc aggctggagt acagtggcgc
     3301 aatctcggct cactgctgcc tccgcttcct gggttcaagt gattcttcct gcctcagctt
     3361 cccaagtagc tgggattaca ggcgcgtgcc accacgctgg ctaatttttg tatttttaat
     3421 agagatgggg tttcaccatg ttggccagga tggtctcaat ctcttgacct tgtgatctgc
     3481 ccaccttggc ttcccaaagt gctgagatta caggtgtgag ccaccaggcc tggccaataa
     3541 ccatttcttg ataaccccac agataatatc ataaactcaa gttgtctgta ttgcttcttc
     3601 ccagactggt cccaagaatg ctgtggttgg ttttgtgtct tttccagcac ttttcttact
     3661 tgctgtcagt tgtcaatctc aacaccttga ccaaaatgaa ctttcttgag tcatttcttc
     3721 tctgtttagc tataagatta gaattcatac tgaaagattt ctaggacttt tgtattaagg
     3781 taggaaagag gaactaggtt ctgggagaaa gactcctttg gaataaaatt catgagcttt
     3841 attggttcct ttaacagatc ttcattgagt ttcatgtgcc aggcatccaa ctagtctctg
     3901 cttttatagt tgtcagtctg acagggctct agtatgtgca caccgtgaca cctgttggct
     3961 gccaggagac tgtttggctt atttcctggt tcttagcaat attccaggtt ggcctggaga
     4021 attccaaagg taaatatgaa aatgtatacc tacaaaagaa tttttgcttt ataggaagaa
     4081 tttgtaggat ttattgagat gtaagctacc tatatacatc aagtatgtga tatgattgta
     4141 aaaagttaca gaagtgtgtg agtcatgaag tacctgtgag ggaagtttat atattttttc
     4201 aaactctttt agtaccattt tcatccacta ggtggaagca attacagtat gacctgcatt
     4261 aaagacatgt acagtttttt ggccggctgc agtggctcac acctgtgatc tcaacacttt
     4321 gggaagctga gacgggagga ttgcttgagc ccaggagttt gagaccagct tgggcaacgt
     4381 gatgaaaccc tgtctctaca aataaaaaaa aaattagcca ggcatggtgg cacaagcctg
     4441 tggtcccagc tactcagaag gctgaggtgg gagtattgat cgagcccagg aggtcaaggc
     4501 tgcagcaagc tgtcgcacca ctgcactcta gcctgggcaa cagcaagacc ctcgtctcaa
     4561 aaaagaaaaa aaaaaagtac aatttttgta ttgattatgt attccttaag taggagtgga
     4621 ataaaagtat tttaccgtgg attggcttct gtgaaggaaa gaagtgaata ttagtagata
     4681 tttggaaaaa agaaagatag ttgatgcagt gattattatt ctgttccttt gtaagttacc
     4741 attagcctct cttgcttaag aaatattttg agcttttttg gggaggggtt gtggttaatg
     4801 atgtgaaatt aatcttcagg aaatttatca taagcagact tgccttttca gatattggta
     4861 tattttaatt ctctacctga atacatacat gacagttatt ttaccatgct ggtttaaaaa
     4921 acatcaattt gaaaatacta gaaggtgggg tgaacactga taagttactg ttaacaaata
     4981 agaaaataat gctttgccat ctgtggtggt tttaaaatct atccacaaat ttttgatatg
     5041 cttctcttta agacgggaca cttcattccc ctctaagtac aggctgagtt tagtgattcc
     5101 cttccaacaa atagatggtg tgtcacttct gagactaggt aataaaaggc attgcatcct
     5161 ccttactctc tcttggatcc ttcactctag ggaaagccag cagccatatt gtaaggccac
     5221 tcaactccat ggagaagacc tagaagtgag gagcagaggc ctccagccaa aagccatgtg
     5281 agggagccat cttggaagca attctccagg cccaaacttc agatgactgt ggtcccagcc
     5341 aatgcattga ccacaacctc atgagagacc ctgagctaga accacctagc taagccactc
     5401 tcaaatatct tatctacaga aactgagatg ataaatgttt gtggtttaa
//
