LOCUS       NM_003635               3758 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens N-deacetylase and N-sulfotransferase 2 (NDST2),
            transcript variant 1, mRNA.
ACCESSION   NM_003635
VERSION     NM_003635.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Duncan,M.B., Liu,M., Fox,C. and Liu,J.
  TITLE     Characterization of the N-deacetylase domain from the heparan
            sulfate N-deacetylase/N-sulfotransferase 2
  JOURNAL   Biochem Biophys Res Commun 339 (4), 1232-1237 (2006)
   PUBMED   16343444
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Carter,N.M., Ali,S. and Kirby,J.A.
  TITLE     Endothelial inflammation: the role of differential expression of
            N-deacetylase/N-sulphotransferase enzymes in alteration of the
            immunological properties of heparan sulphate
  JOURNAL   J Cell Sci 116 (Pt 17), 3591-3600 (2003)
   PUBMED   12876215
REFERENCE   3  (bases 1 to 3758)
  AUTHORS   Yard,B., Feng,Y., Keller,H., Mall,C. and van Der Woude,F.
  TITLE     Influence of high glucose concentrations on the expression of
            glycosaminoglycans and N-deacetylase/N-sulphotransferase mRNA in
            cultured skin fibroblasts from diabetic patients with or without
            nephropathy
  JOURNAL   Nephrol Dial Transplant 17 (3), 386-391 (2002)
   PUBMED   11865082
REFERENCE   4  (bases 1 to 3758)
  AUTHORS   Esko,J.D. and Selleck,S.B.
  TITLE     Order out of chaos: assembly of ligand binding sites in heparan
            sulfate
  JOURNAL   Annu Rev Biochem 71, 435-471 (2002)
   PUBMED   12045103
  REMARK    Review article
REFERENCE   5  (bases 1 to 3758)
  AUTHORS   Humphries,D.E., Lanciotti,J. and Karlinsky,J.B.
  TITLE     cDNA cloning, genomic organization and chromosomal localization of
            human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2
  JOURNAL   Biochem J 332 (Pt 2) (Pt 2), 303-307 (1998)
   PUBMED   9601056
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK303648.1 and BC035711.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_003635.3.
            
            Summary: This gene encodes a member of the
            N-deacetylase/N-sulfotransferase subfamily of the sulfotransferase
            1 proteins. The encoded enzyme has dual functions in processing
            glucosamine and heparin polymers, including N-deacetylation and
            N-sulfation. The encoded protein may be localized to the Golgi.
            [provided by RefSeq, Feb 2009].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035711.1, SRR1803615.259437.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309979.11/ ENSP00000310657.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AK303648.1         234-247
            15-3758             BC035711.1         1-3744
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.2"
     gene            1..3758
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /note="N-deacetylase and N-sulfotransferase 2"
                     /db_xref="GeneID:8509"
                     /db_xref="HGNC:HGNC:7681"
                     /db_xref="MIM:603268"
     exon            1..125
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            126..230
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            231..1576
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             572..3223
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /EC_number="2.8.2.8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     N-heparan sulfate sulfotransferase 2; bifunctional heparan
                     sulfate N-deacetylase/N-sulfotransferase 2;
                     N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2;
                     glucosaminyl N-deacetylase/N-sulfotransferase 2"
                     /codon_start=1
                     /product="bifunctional heparan sulfate
                     N-deacetylase/N-sulfotransferase 2 isoform 1"
                     /protein_id="NP_003626.1"
                     /db_xref="CCDS:CCDS7335.1"
                     /db_xref="GeneID:8509"
                     /db_xref="HGNC:HGNC:7681"
                     /db_xref="MIM:603268"
                     /translation="MLQLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKA
                     KEPLPLPLGDCSSGGAAGPGPARPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEI
                     VAILESSRFRYSTELAPGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRY
                     CVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLE
                     PGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQ
                     RVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADV
                     EALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSH
                     MQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVW
                     GIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSI
                     RGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLA
                     QKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLS
                     LHPAVTSSFPSPSTFEEIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDS
                     EVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQTP
                     LALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLGIT
                     PFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNL
                     ELSKLLSRLGQPVPSWLREELQHSSLG"
     exon            1577..1664
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1819
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..2005
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2134
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2317
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2414
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2538
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2539..2713
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2714..2884
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..2994
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3097
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     exon            3098..3758
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3739..3744
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /note="hexamer: AATAAA"
     polyA_site      3758
                     /gene="NDST2"
                     /gene_synonym="HSST2; N-HSST 2; NST2"
                     /note="major polyA site"
ORIGIN      
        1 agagcgttcg ctcggagatg gcggagcagc cgcgacgcgg cccgtggccc cgaaagcagg
       61 gaagccgggc agccccggga cgcggcggag cccggggaca gcggggacgc cgcccagtcc
      121 tccgggtatg aagctaaaat gcagcggcta agaacgcggc tctggagctt gacaggcctg
      181 acgtcgaatc ccagccctcc caccaactga gctgtgtgat cctgggcaag gtagagggga
      241 agagattgaa ctttgctgac ctttgatgtg aggcgctcag ccagggccaa ggggagagcc
      301 tggcaagatt tgcagcctga agccatgggc cagggggcca tggtgacctg agacaagtgg
      361 actctgtata gttgccccct gcttcccctt ctacctcccc taccctatgc taaggggact
      421 cgtctccacc tcgtaaagga aactccccaa gggaatccct gtcccctatt ttcctatcct
      481 tctacccttc caagacagtc ctagcctata gaactcctac ctcccatccc ctgaggtggt
      541 ccccattcct ccctcccttc ctccccccgc catgctccag ttgtggaagg tggtacgccc
      601 agctcggcag ctggaactgc accgcctcat actgctgctg atcgctttca gcctgggctc
      661 catgggcttc ctggcttatt atgtgtccac cagccctaag gccaaggaac ccttgcccct
      721 gcccttggga gactgcagca gcggtggggc agctggtcct ggccctgcac ggcctccagt
      781 tccacctcgg ccccccaggc ctccagagac agctcgaact gaacccgtgg tccttgtgtt
      841 tgtggagagt gcatactcac agctggggca ggaaattgtg gccatcctgg agtctagtcg
      901 ttttcgttat agcactgagt tggcacctgg ccgaggggac atgcccacat tgactgataa
      961 tacccatggc cgctatgtct tggtcattta tgagaacctg ctcaagtatg tcaacctgga
     1021 tgcctggagt cgggaactgc tagaccggta ctgcgtggag tatggtgtgg gcatcattgg
     1081 ctttttccga gcccacgagc acagcctact gagcgcccag ctcaagggct ttcccctttt
     1141 tttacactca aacttggggc tccgggacta ccaagtgaat ccttctgccc cgctactgca
     1201 tctcacacgc cccagccgcc tagaaccagg gccactgcct ggtgatgact ggaccatctt
     1261 ccaatccaat catagtacat atgaaccagt gcttcttgcc agccttcggc cagctgagcc
     1321 cgcagtgcca ggaccagttc ttcgtcgggc ccggcttccc actgtggtac aggacctggg
     1381 gcttcatgat ggcatccagc gggtgctctt tggacatggc ctttccttct ggctccacaa
     1441 acttatcttc gttgatgctg ttgcatacct cactggcaag cgcctctgcc tggaccttga
     1501 ccgctacatc ttggtagaca tcgatgacat ctttgtgggc aaggaaggga cccgcatgaa
     1561 ggtggctgat gttgaggctc tgttgaccac ccagaacaaa ctcaggacct tagttcccaa
     1621 cttcaccttc aacttgggct tctcgggcaa gttctatcat actgggacag aggaggagga
     1681 tgcaggggac gacatgctgc tgaagcaccg caaagagttc tggtggttcc cccacatgtg
     1741 gagccacatg cagccacacc tgttccacaa tcgctccgtg ctggctgacc agatgaggct
     1801 caacaaacag tttgctctgg agcatgggat tcccacggac ctggggtatg ctgtggcccc
     1861 ccaccactcg ggtgtgtacc ccatccacac gcagctctat gaggcctgga aatccgtgtg
     1921 gggcatccag gtgaccagca ctgaggagta tccccatctc cgccctgccc gctaccgccg
     1981 tggcttcatt cacaatggca ttatggtgct gccccggcag acatgtggcc tcttcactca
     2041 cacaatcttc tataatgagt atcctggagg ctctcgtgaa ctagaccgga gcatccgagg
     2101 tggagagctc tttctgacag tgctgcttaa tccgatcagc atctttatga cccatctgtc
     2161 caattatgga aatgaccggc tgggcctata cacctttgag agcttggtgc gcttcctcca
     2221 gtgttggaca cggctgcgcc tacagaccct tcctcctgtc ccacttgcac agaagtactt
     2281 tgaacttttc cctcaggagc gaagccccct ttggcagaat ccctgtgatg acaagaggca
     2341 caaagatatc tggtccaagg agaaaacctg tgatcgtctc ccgaagttcc tcattgtggg
     2401 accccagaaa acagggacta cagctattca cttcttcctg agcctgcacc cagctgtaac
     2461 tagcagcttc cctagcccca gcacatttga ggagattcag ttcttcaaca gccctaatta
     2521 ccacaagggt attgactggt acatggattt cttccctgtt ccttccaatg ccagcactga
     2581 tttcctattt gaaaaaagtg ccacctactt tgactctgaa gttgtaccac ggcggggggc
     2641 tgccctcctg ccacgagcca agatcatcac agtgctcacc aaccctgctg acagggccta
     2701 ctcctggtac cagcatcagc gagcccatgg agacccagtt gctctgaact ataccttcta
     2761 tcaggtgatt tcagcctcct cccagacccc tctggcacta cgctccctgc agaaccgctg
     2821 tcttgtccct ggctactatt ctacccatct acaacgctgg ctgacttact acccctctgg
     2881 acagttgctg attgtggatg ggcaagagct gcgtaccaac ccagcagcct caatggagag
     2941 catccagaag ttcctgggta tcacaccctt tctgaactac acacggaccc tcaggtttga
     3001 tgatgataag ggattttggt gccagggact tgaaggtggt aagactcgct gtctaggccg
     3061 gagcaaaggc cggaggtatc cagatatgga cactgagtcc cgtcttttcc ttacggattt
     3121 tttccggaac cataatttgg agttgtcgaa gctgctgagc cggcttggac agccagtgcc
     3181 ctcgtggctt cgggaagaac tgcagcattc cagtctgggc tgatgtccca gcctcccata
     3241 ccagcaaaat gccccctgct tccctaaggg gcaggtccag agcagggccc acaaggggga
     3301 ttagagtggc ctggcccctc cccctctacc tcagtagccc ccaggcctga gatggctgag
     3361 aagggaaggg tatccttttc ccacagttct gggacaaata aaggggcttc ctttggtacc
     3421 ccacataata gtgctaggta cctttgaccc atcatcttgg gaggtgggga ggaatgagag
     3481 ggtccaggca gggtgtaggg gaatgtatta gtccaatgag atttccctct tcatccgcag
     3541 cagtgtatct attctatacc tggctatggg agagacccct tgcatgggag ggaccccttg
     3601 ctatggcccc tttagccagg cagtgggatc tacctgtggc ccggcctccc taatgtcatt
     3661 cacattgaat ggggatgagg tcggacagtg gctcatagag ccgagtatga gccctagctg
     3721 tgggctagaa atgtccttaa taaacatcct tatttttc
//
