LOCUS       NM_001543               8011 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens N-deacetylase and N-sulfotransferase 1 (NDST1),
            transcript variant 1, mRNA.
ACCESSION   NM_001543
VERSION     NM_001543.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Missaghian,P., Dierker,T., Khosrowabadi,E., Axling,F., Eriksson,I.,
            Ghanem,A., Kusche-Gullberg,M., Kellokumpu,S. and Kjellen,L.
  TITLE     A dominant negative splice variant of the heparan sulfate
            biosynthesis enzyme NDST1 reduces heparan sulfate sulfation
  JOURNAL   Glycobiology 32 (6), 518-528 (2022)
   PUBMED   35137078
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Khan,A., Miao,Z., Umair,M., Ullah,A., Alshabeeb,M.A., Bilal,M.,
            Ahmad,F., Rappold,G.A., Ansar,M. and Carapito,R.
  TITLE     Two Cases of Recessive Intellectual Disability Caused by NDST1 and
            METTL23 Variants
  JOURNAL   Genes (Basel) 11 (9), 1021 (2020)
   PUBMED   32878022
  REMARK    GeneRIF: Two Cases of Recessive Intellectual Disability Caused by
            NDST1 and METTL23 Variants.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8011)
  AUTHORS   Xue,J., Yang,M., Hua,L.H. and Wang,Z.P.
  TITLE     MiRNA-191 functions as an oncogene in primary glioblastoma by
            directly targeting NDST1
  JOURNAL   Eur Rev Med Pharmacol Sci 23 (14), 6242-6249 (2019)
   PUBMED   31364126
  REMARK    GeneRIF: MiRNA-191 functions as an oncogene in primary glioblastoma
            by directly targeting NDST1.
REFERENCE   4  (bases 1 to 8011)
  AUTHORS   Tanaka,A., Tumkosit,U., Nakamura,S., Motooka,D., Kishishita,N.,
            Priengprom,T., Sa-Ngasang,A., Kinoshita,T., Takeda,N. and Maeda,Y.
  TITLE     Genome-Wide Screening Uncovers the Significance of N-Sulfation of
            Heparan Sulfate as a Host Cell Factor for Chikungunya Virus
            Infection
  JOURNAL   J Virol 91 (13), e00432-17 (2017)
   PUBMED   28404855
  REMARK    GeneRIF: Among the genes enriched in this screening, the authors
            found that TM9SF2 is critical for N-sulfation of heparan sulfate
            and therefore for chikungunya virus infection because it is
            involved in the proper localization and stability of NDST1.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8011)
  AUTHORS   Armstrong,L., Tarailo-Graovac,M., Sinclair,G., Seath,K.I.,
            Wasserman,W.W., Ross,C.J. and van Karnebeek,C.D.
  TITLE     A girl with developmental delay, ataxia, cranial nerve palsies,
            severe respiratory problems in infancy-Expanding NDST1 syndrome
  JOURNAL   Am J Med Genet A 173 (3), 712-715 (2017)
   PUBMED   28211985
  REMARK    GeneRIF: Compound heterozygous mutations in NDST1 were identified,
            in the heparan sulfate N deacetylatase domain of one allele and the
            sulfotransferase domain of the other allele. This report expands
            the phenotypic spectrum of Ndst1 deficiency in humans.
REFERENCE   6  (bases 1 to 8011)
  AUTHORS   Kakuta,Y., Sueyoshi,T., Negishi,M. and Pedersen,L.C.
  TITLE     Crystal structure of the sulfotransferase domain of human heparan
            sulfate N-deacetylase/ N-sulfotransferase 1
  JOURNAL   J Biol Chem 274 (16), 10673-10676 (1999)
   PUBMED   10196134
REFERENCE   7  (bases 1 to 8011)
  AUTHORS   Aikawa,J. and Esko,J.D.
  TITLE     Molecular cloning and expression of a third member of the heparan
            sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family
  JOURNAL   J Biol Chem 274 (5), 2690-2695 (1999)
   PUBMED   9915799
REFERENCE   8  (bases 1 to 8011)
  AUTHORS   Sueyoshi,T., Kakuta,Y., Pedersen,L.C., Wall,F.E., Pedersen,L.G. and
            Negishi,M.
  TITLE     A role of Lys614 in the sulfotransferase activity of human heparan
            sulfate N-deacetylase/N-sulfotransferase
  JOURNAL   FEBS Lett 433 (3), 211-214 (1998)
   PUBMED   9744796
REFERENCE   9  (bases 1 to 8011)
  AUTHORS   Humphries,D.E., Sullivan,B.M., Aleixo,M.D. and Stow,J.L.
  TITLE     Localization of human heparan glucosaminyl
            N-deacetylase/N-sulphotransferase to the trans-Golgi network
  JOURNAL   Biochem J 325 (Pt 2) (Pt 2), 351-357 (1997)
   PUBMED   9230113
REFERENCE   10 (bases 1 to 8011)
  AUTHORS   Dixon,J., Loftus,S.K., Gladwin,A.J., Scambler,P.J., Wasmuth,J.J.
            and Dixon,M.J.
  TITLE     Cloning of the human heparan
            sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher
            Collins syndrome candidate region at 5q32-q33.1
  JOURNAL   Genomics 26 (2), 239-244 (1995)
   PUBMED   7601448
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292448.1, U36600.1, U18918.1
            and AC011383.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001543.4.
            
            Summary: This gene encodes a member of the heparan sulfate/heparin
            GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme
            is a type II transmembrane protein that resides in the Golgi
            apparatus. The encoded protein catalyzes the transfer of sulfate
            from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of
            glucosamine in heparan sulfate. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U36600.1, AK292448.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261797.7/ ENSP00000261797.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-933               AK292448.1         20-952
            934-2144            U36600.1           874-2084
            2145-4159           U18918.1           1878-3892
            4160-8011           AC011383.6         26200-30051
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.1"
     gene            1..8011
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /note="N-deacetylase and N-sulfotransferase 1"
                     /db_xref="GeneID:3340"
                     /db_xref="HGNC:HGNC:7680"
                     /db_xref="MIM:600853"
     exon            1..96
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            97..996
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     CDS             484..3132
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /EC_number="2.8.2.8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     heparan sulfate-N-deacetylase/N-sulfotransferase;
                     N-deacetylase/N-sulfotransferase 1; bifunctional heparan
                     sulfate N-deacetylase/N-sulfotransferase 1; NDST-1; HSNST
                     1; N-HSST 1; N-heparan sulfate sulfotransferase 1;
                     glucosaminyl N-deacetylase/N-sulfotransferase 1;
                     N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1;
                     [Heparan sulfate]-glucosamine N-sulfotransferase 1;
                     N-Deacetylase-N-sulfotransferase 1; heparan
                     sulfate/heparin GlcNAc N-deacetylase/GlcN
                     N-sulfotransferase 1"
                     /codon_start=1
                     /product="bifunctional heparan sulfate
                     N-deacetylase/N-sulfotransferase 1 isoform 1"
                     /protein_id="NP_001534.1"
                     /db_xref="CCDS:CCDS34277.1"
                     /db_xref="GeneID:3340"
                     /db_xref="HGNC:HGNC:7680"
                     /db_xref="MIM:600853"
                     /translation="MPALACLRRLCRHVSPQAVLFLLFIFCLFSVFISAYYLYGWKRG
                     LEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVA
                     ILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCV
                     AYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKG
                     VLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGI
                     QRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVED
                     VKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWS
                     HMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQV
                     WSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKI
                     INGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFKHLVRFLHSWTNLRLQTLPPVQL
                     AQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKTCDRFPKLLIIGPQKTGTTALYLFL
                     GMHPDLSSNYPSSETFEEIQFFNGHNYHKGIDWYMEFFPIPSNTTSDFYFEKSANYFD
                     SEVAPRRAAALLPKAKVLTILINPADRAYSWYQHQRAHDDPVALKYTFHEVITAGSDA
                     SSKLRALQNRCLVPGWYATHIERWLSAYHANQILVLDGKLLRTEPAKVMDMVQKFLGV
                     TNTIDYHKTLAFDPKKGFWCQLLEGGKTKCLGKSKGRKYPEMDLDSRAFLKDYYRDHN
                     IELSKLLYKMGQTLPTWLREDLQNTR"
     exon            997..1491
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1579
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1734
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1920
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2049
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2232
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2329
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2453
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2628
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..2799
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2800..2909
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3012
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..8011
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7989..7994
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /note="hexamer: AATAAA"
     polyA_site      8011
                     /gene="NDST1"
                     /gene_synonym="HSST; MRT46; NST1"
                     /note="major polyA site"
ORIGIN      
        1 ctggccctgg gattaggtgc agccgtgtta aggagaggac attcgagagg gcgtggcgaa
       61 gtgaagagga acctcacgcc actgcctgga gctccgcctt agccccgtgg gccccacgga
      121 gtgagcggca tgcagcaccc ccgggcctgt ccagtgtcct ctggcctgct gccctgcacc
      181 cccagaaggc cctgacgccc tggggcactt ctgctctgca caggaccacg cgggggtttg
      241 ccatggtgac ataaaggggc gcggaggaag gaaggagcgt gaccagcctg tggactgcgc
      301 ccctggctgg gaggaaggac tgggggccca gatcctccac tcccagtgcc ccacaagggc
      361 gtcgcttcct aagtctctgt gaatttgttg gtcagtggac gattctcgtg tctcctcctg
      421 tgtggggcct tggggtagcc agggcaggcc gggcctccgg tggccaaggt ctcggaggcc
      481 aggatgcctg ccctggcatg cctccggagg ctgtgtcggc acgtgtcccc gcaggctgtc
      541 cttttcctgc tgttcatctt ctgcctgttc agcgttttca tctcggccta ctacctatat
      601 ggctggaagc gaggcctgga gccctcggcg gatgcccccg agcctgactg cggggacccg
      661 ccgcctgtgg cccccagtcg cctgctgcca ctcaagcctg tgcaggcagc caccccttcc
      721 cgcacagacc cgttggtgct ggtctttgtg gagagcctct actcgcaact gggccaggag
      781 gtggtggcca tcctggagtc cagccgcttc aaataccgca cagagattgc gccgggcaag
      841 ggtgacatgc ccacgctcac tgacaagggc cgtggccgct tcgccctcat catctatgag
      901 aacatcctca agtatgtcaa cctggacgcc tggaaccggg agctgctgga caagtactgt
      961 gtggcctacg gcgtgggcat cattggcttc ttcaaggcca atgagaacag cctgctgagt
     1021 gcgcagctca agggcttccc cctgttcctg cactcaaacc tgggcctgaa ggactgcagc
     1081 atcaacccca agtccccgct gctctacgtg acgcgaccta gcgaggtgga gaaaggtgtg
     1141 ctccccggcg aggactggac ggttttccag tcaaatcact ccacctatga gccagtgctg
     1201 ctggccaaga cgcgctcgtc tgagtccatc ccacacctgg gcgcagacgc cggcctgcat
     1261 gctgcactgc acgccactgt ggtccaggac ctgggcctgc acgacggcat ccagcgcgtg
     1321 ctgtttggca acaacctgaa cttctggctg cacaagcttg tcttcgtgga tgccgtggcc
     1381 ttcctcacgg ggaagcgcct ctccctgcca ttggaccgct acatcctggt ggacattgat
     1441 gacatcttcg tgggcaagga gggcacacgc atgaaggtgg aggacgtgaa ggccctgttt
     1501 gacacacaga acgaactacg cgcacacatc ccaaacttca ccttcaacct gggctactca
     1561 gggaaattct tccacacagg taccaatgct gaggacgctg gggatgatct gctgctgtcg
     1621 tatgtgaagg agttctggtg gttcccccac atgtggagcc acatgcagcc ccaccttttc
     1681 cacaaccagt ccgtgttggc cgagcagatg gccttgaaca agaagttcgc tgtcgagcat
     1741 ggcattccca cagacatggg gtatgcagtg gcgccccacc actcgggcgt gtaccccgtg
     1801 cacgtgcagc tgtacgaggc ttggaagcag gtgtggagca tccgcgtgac cagcacggag
     1861 gagtaccccc acctgaagcc agcccgctac cgccgtggct tcatccacaa tggcatcatg
     1921 gttctcccac ggcagacctg cggcctcttc acacacacca tcttctacaa cgagtaccct
     1981 ggcggctcca gtgagctgga caagatcatc aacgggggcg agctcttcct caccgtgctc
     2041 ctcaatccta tcagcatctt catgacgcac ctgtccaact atgggaatga ccgcctgggc
     2101 ctgtacacct tcaagcacct ggtgcgcttc ctgcactcct ggacgaacct ccggctgcag
     2161 acactgcccc ctgtgcagtt ggcgcagaag tacttccaga tcttctccga ggagaaggac
     2221 ccgctctggc aggacccctg cgaggacaaa cgtcacaaag acatctggtc caaggagaag
     2281 acgtgtgacc gcttcccaaa gctcctcatc atcggccccc agaaaacagg caccactgcc
     2341 ctctacctgt tcctgggcat gcaccctgac ctaagcagca actaccccag ctctgagacc
     2401 tttgaggaga tccagttttt taatggccac aactatcaca aaggcatcga ctggtacatg
     2461 gagttcttcc ccatcccttc caacaccacc tccgacttct actttgagaa aagcgccaac
     2521 tactttgatt cagaagtggc gccccggcgg gcagcagccc tcttgcccaa agccaaggtc
     2581 ctgaccatcc tcatcaaccc cgcggaccgg gcctattcct ggtaccagca ccagcgagcc
     2641 catgacgacc cagtggccct aaagtacacc ttccatgagg tgattaccgc cggctctgac
     2701 gcatcctcga agctgcgtgc cctccagaac cgctgcctgg tccctggctg gtacgccacc
     2761 cacatcgagc gctggctcag tgcctatcac gccaaccaga ttctggtctt ggatggcaaa
     2821 ctgcttcgca cagaacctgc caaagtgatg gacatggtgc agaagttcct tggggtgacc
     2881 aacaccattg actaccacaa aaccttggcg tttgatccaa agaaaggatt ttggtgccaa
     2941 ctgcttgaag gaggaaaaac caagtgtctg ggcaaaagca agggccggaa atatcccgag
     3001 atggacttgg attcccgagc cttcctgaag gactattacc gggaccacaa catcgagctc
     3061 tccaagctgc tgtataagat gggccagaca cttcccactt ggctacgaga ggacctccag
     3121 aacaccaggt agccgtggcc accacagcca gactgaacgt ttgtgaaagc tgggacatcc
     3181 caccacacgc tgagccagac ctgcagagtg ggaagctgga ccagggcagc tgcgcactta
     3241 tgagcaatac tctgtggagg tctggtgggg ctgggggagc acccaggcgg atctgcaagc
     3301 acctcggagc acccaccgct gggtctgcgg cctaagggac ctccctcgcc agcagaggtc
     3361 cattccgttc ccagctgctc ctggggaggc cgcttcctgg taggagggag tccacgagac
     3421 tcttttctgt ccctcactgt gttccgccga ctgtcccctc tcgtcaccca tcactccctg
     3481 cttccgcagg gcgcccctca gtattcgctg ccatatgtcc ctgtcctcca ggctgtaggg
     3541 gaggagagcc tggccggggg agacagactg gacatttccc tgtttcgagc caggctcttc
     3601 caaggggcca gctgggtccc cggagtcagt cctaggctgg atgggagggt ggccccctca
     3661 agaggactcc cagcctccac atctggttcc taccttcaca tctcaccctc ccgttctggg
     3721 gaagaatttc tggttcctac agtatccact ccatcctcaa ggcttcccgc agggccttgg
     3781 ggcactgcct tgccatcggg cccagttctc cgggccccac ctgcacccct ttcttccccc
     3841 tgggatatga tgtgtggtgt ttcctgtggt aaaagactga ggcaggccag gggtctgcca
     3901 gtaacatgtt cccatgtaca gacacggtcc ccacaccctc ccagcctcag gcccaggcag
     3961 acatgggcga gctggtgaga ctgccagcca cggctttgct tagccacctg tggccgaggg
     4021 ctctcagaga cccccttaac ctcccaaata ctaagaagct aaaatatttt aatattttgt
     4081 tttttttttt cttggtgcca gagtttatac cctgggtgct ggggtcgcac tgtgttatat
     4141 atatatatat atatatataa tgtgtatata tatatattct attttttttg gttgggttcg
     4201 tttttaattc agtcctacaa aatggtggta agccccagtc tcaggggtca ccctgtctta
     4261 gagctagaga tgaggtggca gggagggagg ctgtgtcctc agtggcctgg ggctgagcag
     4321 accttaggaa ggggccctcc ccagtgccgt ggctgctctg gggctacccc aggtgaacag
     4381 aggtggtgag atgcgcaggg aagaccagct ctctccagga catcgggctg cgtgaagtcc
     4441 agtgaccctg aggggatttt ggccaaagga aacagccagg gctgggctag gagagctggg
     4501 gtcaaaagct gccccaccca ctctccctca catggtgcta ggctgggagc tgccctgaga
     4561 gctgggatgc ccaccaaggc ccacagaact gtctccaggc ccttgtagac agtgggatct
     4621 ctgggctgat ttctggttgg ggtctgctcc taccccaccc catttaacaa tgtgaagtga
     4681 ctgaggctgg ggtgcctctc cctggccccc tccaactgcc aaattctgct tccctctgaa
     4741 ccccaggaaa tactttctgg agaggcttgt cttgttcaga agactctagc atcttggggc
     4801 tggaaggtcc tttgacgggt atgaaacagg tccagaaagg ggaggtggcc tgcttggccg
     4861 acgtcatgga gccagctggg agcagggctg agcgttgaac ccgaggctcc cggttccaag
     4921 tcccatcgtg ctcctcttcc aatgccacac tgcctctgag cccatccttg ggaccctact
     4981 ctgtgccagg ttcctgtccc ttcctccgtc acagcatggt cattccagga tatggggaca
     5041 aaccactcct tcccagccag ggtgcctttg agccttctca ttccgaggtc tgcgtttgca
     5101 tccctgtcgt gttctccctc gaggggtcct tccccagcct gtgggggcct gggtctgttc
     5161 tgagctgatt cccttttctc cagttctctg ctgggatgaa gaaagttgga cactcagtgg
     5221 gaccagctgc aggtggtttc actgtgtaca cagcagtgtc cccatcatgc ctctcaatgg
     5281 ggcctgcaag cctgggaact tgggcatcgt tgggccagca ggatgagcag caaagggtct
     5341 gaggacaggg tggcatgtct gcttgtgtgc tcatgaagac atgcccacgc catggagcca
     5401 ggggcttata gagaggggca gtgacttcca gagtcccatc cgtcagtgag gggactgggc
     5461 tgcgggatgg gtgaggatct tacagctccc aggttcggaa atctaccctc taggcctgga
     5521 ggcctctctc gcagagcatg gaaggcagga gaggtgggct ggcctagggc aggagctcca
     5581 cttctctctg cctctggcta tgggaaggag caggcggttg gaaatcctgt gacagcagag
     5641 atcttctgga tttttgcctg gacccttctg caagcctgga gactggacct gccacatgtt
     5701 ccaagtctgt ctgcctaggt cactgctgcc acttctcctc ccactaacag acctttcttg
     5761 aagacccact aggtccagac actgccggga cttgcaggta tggggggtag aaggagaggc
     5821 agataagctg cctggggggc tgttagctaa tgggtacaag gacaccccct cccgagaaga
     5881 gaagccacag atgagtgggc cagcgaggcc agtcttcaat gtccactaat tcactctaag
     5941 catttggcca aaaaaaggag agagaaataa ctgaaattgt tcaggtggct ccatcgtcta
     6001 ttctgtggtc ccctccatga gcctttgccc cagggtgggt gcaggtgcga gtgtcacctg
     6061 cagctgcctc agtcattcag agttccagag ccttctgtgg tggagcatcc tgctgcttgg
     6121 aggatgagtc tgaagtttaa aaatgggcct tttctgagcc cccctgatga gcagtcagtg
     6181 cttctgggcc cagctaagga agccatctgt tcccagcaga ggagagctgc tgcgctgacc
     6241 tgcagaggtt ggtggtccca gcacagagct gtgcccatgg gcttgttagt gggaaaagca
     6301 aatacaacaa tgttttacca aacatttaat tatgaatgcc cactccccag catctagatc
     6361 taaaattatt aacattttgc cacattcttt ttctctctct gtgtatgtat atgttttctc
     6421 tgtcctaatt taaaagtaca ttgcaaacat catgaaactt gatccctgaa agcttcagcc
     6481 tgcatctcct aagagttagg gcattgtcct acacattggc ccagtatcac agccaagacg
     6541 agaagtcatt tcccagcgat gtctgctatc taatctgtat tcaaatcccc ccaattgtct
     6601 cccaggtaac ttatgtgtct ggttttggaa tcagcatcca gtcacggtgc acacattgca
     6661 ttttctaact atgtttttaa aacctctttg aatgctgaaa ggtcttccct cagctcactt
     6721 tgagttttta aatgacattg tttttgaggc cctaggcctg ctgtcttttt gaagccccac
     6781 attctgaatt tgtcagattg tttgctcatg gtgtcgcata accacttctt ctatcccctg
     6841 tactttcaag gataatttag gctacatgag attttgcctt ttgtgctgac ttgacagctt
     6901 cacttctgcc taagatgcgc ctccactgtc acctgtacca agtgccaaaa cagcagaggg
     6961 gccagttaga gcaagagctc tgggggggcc ggaaagtctc cctggagaag gccgtaatat
     7021 ctgggccttg gaaggaagga cacagggctc tggaaggagg aggctgcatg cctgaaggct
     7081 gggccttcga ccctggggat gggccacagg gcaccaaaca catcatatgt ctctctcttt
     7141 ctattttggg gtcccagggt tttcatttcc tggagacccg tggtctgtgt gcactggagc
     7201 agagccaggc tctcagttgc agcagggaaa agcctacatg gggaactgcc tcccgcaagc
     7261 tgtggtgtga gaagagccct ggctggcagc aggagctgtg aagtttcatc ccagccgggt
     7321 gcatttagta gctgcctgac catcttcctc ccaggccctc agtttcttca tctgtagaat
     7381 gagcagatag gggccacagc agggattttc acacatcaaa ggaggcagcc tctgctctag
     7441 ttgatgtgag gatgaggggg cggcctagac cccctgctgc ccaggccgtt gaggcaacac
     7501 tgggttcctg gcttgacagc tgtgggctgg ctgcccttgc cagctcagac ggctggcgag
     7561 ttttcagctt ccctaaggga ctggcatagg gttttgtggg gtcaatgcca ggaacctggg
     7621 ggaaactgga tgcgttttat cctaagaagt tcatccctcc tgctcttact gtttccggcc
     7681 ctggaggaga atgagggagg agagggtttt ctatgactgt gttagctttt attattagca
     7741 agagggctcc ttttgtaatt tgtgcatgaa attcatgtca tttcctggga gaggagaggg
     7801 ccgactggag agggtggggg gcacactggg gaagcaggca gaggtttcct tcccttgtag
     7861 gggggtgggg agagtgggga gagggggtgc tatccagttc gcctgatgag ggctctcgaa
     7921 ccagccgttt tgggttgtgt taaaggtgag accttcctcc attaatgtac aatctcgaac
     7981 taactgctaa taaagtgggg ttctgtttgt a
//
