LOCUS       NM_001242835            3208 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens NDRG family member 4 (NDRG4), transcript variant 6,
            mRNA.
ACCESSION   NM_001242835
VERSION     NM_001242835.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Zuo,H., Liu,S., Li,X. and Hou,G.
  TITLE     miR-23a-3p promotes the development of colon cancer by inhibiting
            the expression of NDRG4
  JOURNAL   Clin Transl Oncol 25 (4), 933-940 (2023)
   PUBMED   36374403
  REMARK    GeneRIF: miR-23a-3p promotes the development of colon cancer by
            inhibiting the expression of NDRG4.
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Yang,Z., Cheng,H., Zhang,Y. and Zhou,Y.
  TITLE     Errate: Identification of NDRG Family Member 4 (NDRG4) and CDC28
            Protein Kinase Regulatory Subunit 2 (CKS2) as Key Prognostic Genes
            in Adrenocortical Carcinoma by Transcriptomic Analysis
  JOURNAL   Med Sci Monit 28, e937786 (2022)
   PUBMED   35899496
  REMARK    GeneRIF: Errate: Identification of NDRG Family Member 4 (NDRG4) and
            CDC28 Protein Kinase Regulatory Subunit 2 (CKS2) as Key Prognostic
            Genes in Adrenocortical Carcinoma by Transcriptomic Analysis.
            Correction to:[Med Sci Monit. 2021 Mar 05;27:e928523. doi:
            10.12659/MSM.928523.. PMID: 33667214]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3208)
  AUTHORS   Ji,X., Sha,J., Qian,H., Zhang,G., He,T. and Dang,Y.
  TITLE     Highly sensitive fecal DNA testing of NDRG4 12b methylation is a
            promising marker for detection of colorectal precancerosis
  JOURNAL   J BUON 26 (4), 1239-1245 (2021)
   PUBMED   34564976
  REMARK    GeneRIF: Highly sensitive fecal DNA testing of NDRG4 12b
            methylation is a promising marker for detection of colorectal
            precancerosis.
REFERENCE   4  (bases 1 to 3208)
  AUTHORS   Vaes,N., Schonkeren,S.L., Rademakers,G., Holland,A.M., Koch,A.,
            Gijbels,M.J., Keulers,T.G., de Wit,M., Moonen,L., Van der
            Meer,J.R.M., van den Boezem,E., Wolfs,T.G.A.M., Threadgill,D.W.,
            Demmers,J., Fijneman,R.J.A., Jimenez,C.R., Vanden Berghe,P.,
            Smits,K.M., Rouschop,K.M.A., Boesmans,W., Hofstra,R.M.W. and
            Melotte,V.
  TITLE     Loss of enteric neuronal Ndrg4 promotes colorectal cancer via
            increased release of Nid1 and Fbln2
  JOURNAL   EMBO Rep 22 (6), e51913 (2021)
   PUBMED   33890711
  REMARK    GeneRIF: Loss of enteric neuronal Ndrg4 promotes colorectal cancer
            via increased release of Nid1 and Fbln2.
REFERENCE   5  (bases 1 to 3208)
  AUTHORS   Yang,Z., Cheng,H., Zhang,Y. and Zhou,Y.
  TITLE     Identification of NDRG Family Member 4 (NDRG4) and CDC28 Protein
            Kinase Regulatory Subunit 2 (CKS2) as Key Prognostic Genes in
            Adrenocortical Carcinoma by Transcriptomic Analysis
  JOURNAL   Med Sci Monit 27, e928523 (2021)
   PUBMED   33667214
  REMARK    GeneRIF: Identification of NDRG Family Member 4 (NDRG4) and CDC28
            Protein Kinase Regulatory Subunit 2 (CKS2) as Key Prognostic Genes
            in Adrenocortical Carcinoma by Transcriptomic Analysis.
            Erratum:[Med Sci Monit. 2022 Jul 28;28:e937786. doi:
            10.12659/MSM.937786.. PMID: 35899496]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3208)
  AUTHORS   Hongo,S., Watanabe,T., Takahashi,K. and Miyazaki,A.
  TITLE     Ndrg4 enhances NGF-induced ERK activation uncoupled with Elk-1
            activation
  JOURNAL   J Cell Biochem 98 (1), 185-193 (2006)
   PUBMED   16408304
  REMARK    GeneRIF: NDRG4 overexpression enhances ERK activation
REFERENCE   7  (bases 1 to 3208)
  AUTHORS   Nishimoto,S., Tawara,J., Toyoda,H., Kitamura,K. and Komurasaki,T.
  TITLE     A novel homocysteine-responsive gene, smap8, modulates mitogenesis
            in rat vascular smooth muscle cells
  JOURNAL   Eur J Biochem 270 (11), 2521-2531 (2003)
   PUBMED   12755708
  REMARK    GeneRIF: smap8 is involved in the regulation of mitogenic
            signalling in vascular smooth muscle cells, possibly in response to
            a homocysteine-induced injury [SMAP8]
REFERENCE   8  (bases 1 to 3208)
  AUTHORS   Ohki,T., Hongo,S., Nakada,N., Maeda,A. and Takeda,M.
  TITLE     Inhibition of neurite outgrowth by reduced level of NDRG4 protein
            in antisense transfected PC12 cells
  JOURNAL   Brain Res Dev Brain Res 135 (1-2), 55-63 (2002)
   PUBMED   11978393
REFERENCE   9  (bases 1 to 3208)
  AUTHORS   Qu,X., Zhai,Y., Wei,H., Zhang,C., Xing,G., Yu,Y. and He,F.
  TITLE     Characterization and expression of three novel
            differentiation-related genes belong to the human NDRG gene family
  JOURNAL   Mol Cell Biochem 229 (1-2), 35-44 (2002)
   PUBMED   11936845
  REMARK    GeneRIF: Cloning and expression of the gene; specifically expressed
            in brain and heart
REFERENCE   10 (bases 1 to 3208)
  AUTHORS   Zhou,R.H., Kokame,K., Tsukamoto,Y., Yutani,C., Kato,H. and
            Miyata,T.
  TITLE     Characterization of the human NDRG gene family: a newly identified
            member, NDRG4, is specifically expressed in brain and heart
  JOURNAL   Genomics 73 (1), 86-97 (2001)
   PUBMED   11352569
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB044945.1 and BC011795.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 14, 2020 this sequence version replaced NM_001242835.1.
            
            Summary: This gene is a member of the N-myc downregulated gene
            family which belongs to the alpha/beta hydrolase superfamily. The
            protein encoded by this gene is a cytoplasmic protein that is
            required for cell cycle progression and survival in primary
            astrocytes and may be involved in the regulation of mitogenic
            signalling in vascular smooth muscles cells. Alternative splicing
            results in multiple transcripts encoding different
            isoforms.[provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (6) has multiple differences at
            the 5' end which result in the use of a distinct translation
            initiation codon and an additional exon in the 3' coding region,
            compared to variant 2. The encoded protein (isoform 6; also known
            as NDRG4-Bvar) has a shorter and distinct N-terminus and longer
            C-terminus, compared to isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB044945.1, AB033006.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000570248.6/ ENSP00000457659.1
            RefSeq Select criteria :: based on manual assertion
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2506              AB044945.1         1-2506
            2507-2786           BC011795.2         2456-2735
            2787-3208           BC011795.2         2740-3161
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q21"
     gene            1..3208
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /note="NDRG family member 4"
                     /db_xref="GeneID:65009"
                     /db_xref="HGNC:HGNC:14466"
                     /db_xref="MIM:614463"
     exon            1..127
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1165
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /note="isoform 6 is encoded by transcript variant 6;
                     smooth muscle-associated protein 8; protein NDRG4; brain
                     development-related molecule 1; vascular smooth muscle
                     cell-associated protein 8; N-myc downstream-regulated gene
                     4 protein"
                     /codon_start=1
                     /product="protein NDRG4 isoform 6"
                     /protein_id="NP_001229764.1"
                     /db_xref="CCDS:CCDS58466.1"
                     /db_xref="GeneID:65009"
                     /db_xref="HGNC:HGNC:14466"
                     /db_xref="MIM:614463"
                     /translation="MPECWDGEHDIETPYGLLHVVIRGSPKGNRPAILTYHDVGLNHK
                     LCFNTFFNFEDMQEITKHFVVCHVDAPGQQVGASQFPQGYQFPSMEQLAAMLPSVVQH
                     FGFKYVIGIGVGAGAYVLAKFALIFPDLVEGLVLVNIDPNGKGWIDWAATKLSGLTST
                     LPDTVLSHLFSQEELVNNTELVQSYRQQIGNVVNQANLQLFWNMYNSRRDLDINRPGT
                     VPNAKTLRCPVMLVVGDNAPAEDGVVECNSKLDPTTTTFLKMADSGGLPQVTQPGKLT
                     EAFKYFLQGMGYIAYLKDRRLSGGAVPSASMTRLARSRTASLTSASSVDGSRPQACTH
                     SESSEGLGQVNHTMEVSC"
     exon            128..233
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            234..354
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            355..417
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            418..478
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            479..565
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            566..622
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            623..726
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            727..783
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            784..835
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            836..883
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            884..919
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            920..971
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1010
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..3208
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2985..2990
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /note="hexamer: AATAAA"
     polyA_site      3004
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /note="major polyA site"
     regulatory      3188..3193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
                     /note="hexamer: ATTAAA"
     polyA_site      3208
                     /gene="NDRG4"
                     /gene_synonym="BDM1; SMAP-8; SMAP8"
ORIGIN      
        1 acagagccga ccatctccca ctcgagctgc ccccgccctc tggacccgag tgactcaggc
       61 ctttgtttgt ccttcctggt agaggcgggt tccctccctc ggcaagatgc cggagtgctg
      121 ggatggggaa catgacatcg agacacccta cggccttctg catgtagtga tccggggctc
      181 ccccaagggg aaccgcccag ccatcctcac ctaccatgat gtgggcctca accacaaact
      241 atgcttcaac accttcttca acttcgagga catgcaggag atcaccaagc actttgtggt
      301 gtgtcacgtg gatgcccctg gacaacaggt gggggcgtcg cagtttcctc aggggtacca
      361 gttcccctcc atggagcagc tggctgccat gctccccagc gtggtgcagc atttcgggtt
      421 caagtatgtg attggcatcg gagtgggcgc cggagcctat gtgctggcca agtttgcact
      481 catcttcccc gacctggtgg aggggctggt gctggtgaac atcgacccca atggcaaagg
      541 ctggatagac tgggctgcca ccaagctctc cggcctaact agcactttac ccgacacggt
      601 gctctcccac ctcttcagcc aggaggagct ggtgaacaac acagagttgg tgcagagcta
      661 ccggcagcag attgggaacg tggtgaacca ggccaacctg cagctcttct ggaacatgta
      721 caacagccgc agagacctgg acattaaccg gcctggaacg gtgcccaatg ccaagacgct
      781 ccgctgcccc gtgatgctgg tggttgggga taatgcaccc gctgaggacg gggtggtgga
      841 gtgcaactcc aaactggacc cgaccactac gaccttcctg aagatggcag actctggagg
      901 gctgccccag gtcacacagc cagggaagct gactgaagcc ttcaaatact tcctgcaagg
      961 catgggctac attgcgtact tgaaggaccg aaggctgagt ggaggagcag tgccctcagc
     1021 cagcatgacc cgcctggcac gctcccgcac tgcatccctc accagtgcca gctcggtgga
     1081 tggcagccgc ccacaggcct gcacccactc agagagcagc gaggggctgg gccaggtcaa
     1141 ccacaccatg gaggtgtcct gttgaagccc ttgatcccgc tgacgacgcc cacgtcgagg
     1201 ccccaccgcc atccttgcgc cggctcatgt tccctttagt ttatttttgt gagggcaaag
     1261 gggaggaaat ggggttctgt ttgaaaaaaa tgaggggatc ttagatgctg cagcagaaca
     1321 gtctccaggt gttttaaggg gctcagtcct cctcatccca tctcactctc cgtggtaact
     1381 tagccaactt gacccctctc atcccactcc cggcggccca ggcacagaag ggcagggcca
     1441 tagggaggga gattcgctac ggatccaggc cattcctggg tgagcccttg ggcaggcatg
     1501 tttggagatg agagaggctt cgagagggtg ggtgctgggc cacaggggtg cggggccagc
     1561 tcaggcactg gcgtgggagc cctgggagac cccttccccc accctccacc aagcacacct
     1621 gtttctgtct catagcacat gtgacaatca tctggacaac agccacaagg gggcgctcgg
     1681 accaggcagc cactttcctg gtgctctctg ggcccagctg gtgctgtagg gccacgcagg
     1741 caggggcgtc aaggggtttc tctgcccaag gaagacagaa catggagaac cgtcagggca
     1801 ggaaccccac agactgtccc ttccagccca cactctgcca cctcctggcc ctgtcccaat
     1861 tctgagccaa ggcctccccg aggcagaagt tgcctggtcc tctgtcccca cagtgacctg
     1921 actgggggtg agggagaagg aggagagagc ccatgtgtgg tgtgtgtgcc cctgagaact
     1981 tcgtggtgac tgcctttggg agcccgcagg tggccagagg caggggtagc tgagttcctg
     2041 gagacccctt ttttgccccc aggttcccca gagggcaacg ccatcagtag cagtgtggtg
     2101 tttcaggcag agctctggcc aggctgtgcc agtgtgtccc ggacgcatca ctaaggaaga
     2161 gagagtttat ttagtcaact ggcccaaggc agcgaggctt ctacagtccc acaccccata
     2221 gccgcctggg ctggggctta ctgggggctg aaggttctgg acatgaacaa gggtcaggta
     2281 gaagagaaag gcttccccta caccccagcc tcctgctgtc ccctgaagcc caggactgcg
     2341 ttgtatgctt tccatccact caccttaccc catagcatct tgcggcccag aaaccagagc
     2401 catttgtctc agaccctaaa tcaataatca caaaccccaa aacgggagag agcagtgaaa
     2461 acatgcaggg ctgtggacgg gggaagggtt gtggcgggtg ttctgaggct gagaggacac
     2521 ctatatgcgt atttcctcta cacacatcac cccccttcta taatcttaag ccatgactag
     2581 cctggtggcg tgttagtttc tgcccagttc taccccctca tgtgcttctt ctgaatactg
     2641 aatgtgactg tttgaaagct ggtagaattc atccctctta ctgtagataa cactgcaaat
     2701 cttggaattt tgttttttgc tgtttccaga tgtatctata aatatctata cattatatgt
     2761 gtgtgtgtgt gtgtgtgtgt gtgtgtacat cgggtcctcc catgtgtggt gttcttctgg
     2821 aggttgtctc tttggtcaag gtgaactttt aatgtttatt attttcttct ccgcacaaag
     2881 taaagagcct aattttgtgt attctggtgg ctgctgtcat gagatgataa aatgtaaaac
     2941 aaaactctag tcaacgtaga aagagttaac tgtgctgaaa aactaataaa gaacctaaga
     3001 agaattccag tgtggtgatg ccatgcccat catgggaggc ttttggagaa acagaatgtt
     3061 tgggcagggg ctgctggtgc tgcttgggtt ttgggttgag ggtgctagga gaggatggtc
     3121 tccacccatc tttctatttc cagtacacgt cacattattt taccggtgag atgagaatgt
     3181 cacaaacatt aaaagcctta tgtgctca
//
