LOCUS       NM_006101               2126 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens NDC80 kinetochore complex component (NDC80), mRNA.
ACCESSION   NM_006101
VERSION     NM_006101.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Wang,Y., Wang,B., Zhou,F., Lv,K., Xu,X. and Cao,W.
  TITLE     CircNDC80 promotes glioblastoma multiforme tumorigenesis via the
            miR-139-5p/ECE1 pathway
  JOURNAL   J Transl Med 21 (1), 22 (2023)
   PUBMED   36635757
  REMARK    GeneRIF: CircNDC80 promotes glioblastoma multiforme tumorigenesis
            via the miR-139-5p/ECE1 pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Li,J., Xu,X. and Peng,X.
  TITLE     NDC80 Enhances Cisplatin-resistance in Triple-negative Breast
            Cancer
  JOURNAL   Arch Med Res 53 (4), 378-387 (2022)
   PUBMED   35346500
  REMARK    GeneRIF: NDC80 Enhances Cisplatin-resistance in Triple-negative
            Breast Cancer.
            Erratum:[Arch Med Res. 2023 Jul;54(5):102833. doi:
            10.1016/j.arcmed.2023.05.001.. PMID: 37193619]
REFERENCE   3  (bases 1 to 2126)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 2126)
  AUTHORS   Iemura,K., Natsume,T., Maehara,K., Kanemaki,M.T. and Tanaka,K.
  TITLE     Chromosome oscillation promotes Aurora A-dependent Hec1
            phosphorylation and mitotic fidelity
  JOURNAL   J Cell Biol 220 (7) (2021)
   PUBMED   33988677
  REMARK    GeneRIF: Chromosome oscillation promotes Aurora A-dependent Hec1
            phosphorylation and mitotic fidelity.
REFERENCE   5  (bases 1 to 2126)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2126)
  AUTHORS   Zheng,L., Chen,Y., Riley,D.J., Chen,P.L. and Lee,W.H.
  TITLE     Retinoblastoma protein enhances the fidelity of chromosome
            segregation mediated by hsHec1p
  JOURNAL   Mol Cell Biol 20 (10), 3529-3537 (2000)
   PUBMED   10779342
REFERENCE   7  (bases 1 to 2126)
  AUTHORS   Zheng,L., Chen,Y. and Lee,W.H.
  TITLE     Hec1p, an evolutionarily conserved coiled-coil protein, modulates
            chromosome segregation through interaction with SMC proteins
  JOURNAL   Mol Cell Biol 19 (8), 5417-5428 (1999)
   PUBMED   10409732
REFERENCE   8  (bases 1 to 2126)
  AUTHORS   Chen,Y., Riley,D.J., Chen,P.L. and Lee,W.H.
  TITLE     HEC, a novel nuclear protein rich in leucine heptad repeats
            specifically involved in mitosis
  JOURNAL   Mol Cell Biol 17 (10), 6049-6056 (1997)
   PUBMED   9315664
REFERENCE   9  (bases 1 to 2126)
  AUTHORS   Chen,Y., Sharp,Z.D. and Lee,W.H.
  TITLE     HEC binds to the seventh regulatory subunit of the 26 S proteasome
            and modulates the proteolysis of mitotic cyclins
  JOURNAL   J Biol Chem 272 (38), 24081-24087 (1997)
   PUBMED   9295362
REFERENCE   10 (bases 1 to 2126)
  AUTHORS   Durfee,T., Becherer,K., Chen,P.L., Yeh,S.H., Yang,Y., Kilburn,A.E.,
            Lee,W.H. and Elledge,S.J.
  TITLE     The retinoblastoma protein associates with the protein phosphatase
            type 1 catalytic subunit
  JOURNAL   Genes Dev 7 (4), 555-569 (1993)
   PUBMED   8384581
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP005136.2 and BC035617.1.
            
            On Nov 23, 2018 this sequence version replaced NM_006101.2.
            
            Summary: This gene encodes a component of the NDC80 kinetochore
            complex. The encoded protein consists of an N-terminal microtubule
            binding domain and a C-terminal coiled-coiled domain that interacts
            with other components of the complex. This protein functions to
            organize and stabilize microtubule-kinetochore interactions and is
            required for proper chromosome segregation. [provided by RefSeq,
            Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK289396.1, SRR5189661.140810.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261597.9/ ENSP00000261597.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AP005136.2         147012-147034
            24-2126             BC035617.1         1-2103
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.32"
     gene            1..2126
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /note="NDC80 kinetochore complex component"
                     /db_xref="GeneID:10403"
                     /db_xref="HGNC:HGNC:16909"
                     /db_xref="MIM:607272"
     exon            1..127
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            128..237
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..2065
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /note="highly expressed in cancer, rich in leucine heptad
                     repeats; kinetochore associated 2; NDC80 homolog,
                     kinetochore complex component; NDC80 kinetochore complex
                     component homolog; kinetochore protein Hec1;
                     kinetochore-associated protein 2; highly expressed in
                     cancer protein; retinoblastoma-associated protein HEC"
                     /codon_start=1
                     /product="kinetochore protein NDC80 homolog"
                     /protein_id="NP_006092.1"
                     /db_xref="CCDS:CCDS11827.1"
                     /db_xref="GeneID:10403"
                     /db_xref="HGNC:HGNC:16909"
                     /db_xref="MIM:607272"
                     /translation="MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLS
                     INKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEF
                     LTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGY
                     PFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDG
                     IMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALN
                     EQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEE
                     IARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQ
                     KLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCL
                     VKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQ
                     KLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQL
                     VVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIK
                     EIRDKYEKKATLIKSSEE"
     exon            238..315
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            316..439
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            440..612
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            613..715
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            716..805
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            806..899
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1006
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1151
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1357
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1510
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1600
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1693
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1824
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1927
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2126
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2102..2107
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /note="hexamer: AATAAA"
     polyA_site      2126
                     /gene="NDC80"
                     /gene_synonym="HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3"
                     /note="major polyA site"
ORIGIN      
        1 aaattcgaac ggctttggcg ggccgaggaa ggacctggtg ttttgatgac cgctgtcctg
       61 tctagcagat acttgcacgg tttacagaaa ttcggtccct gggtcgtgtc aggaaactgg
      121 aaaaaaggtc ataagcatga agcgcagttc agtttccagc ggtggtgctg gccgcctctc
      181 catgcaggag ttaagatccc aggatgtaaa taaacaaggc ctctataccc ctcaaaccaa
      241 agagaaacca acctttggaa agttgagtat aaacaaaccg acatctgaaa gaaaagtctc
      301 gctatttggc aaaagaacta gtggacatgg atcccggaat agtcaacttg gtatattttc
      361 cagttctgag aaaatcaagg acccgagacc acttaatgac aaagcattca ttcagcagtg
      421 tattcgacaa ctctgtgagt ttcttacaga aaatggttat gcacataatg tgtccatgaa
      481 atctctacaa gctccctctg ttaaagactt cctgaagatc ttcacatttc tttatggctt
      541 cctgtgcccc tcatacgaac ttcctgacac aaagtttgaa gaagaggttc caagaatctt
      601 taaagacctt gggtatcctt ttgcactatc caaaagctcc atgtacacag tgggggctcc
      661 tcatacatgg cctcacattg tggcagcctt agtttggcta atagactgca tcaagataca
      721 tactgccatg aaagaaagct cacctttatt tgatgatggg cagccttggg gagaagaaac
      781 tgaagatgga attatgcata ataagttgtt tttggactac accataaaat gctatgagag
      841 ttttatgagt ggtgccgaca gctttgatga gatgaatgca gagctgcagt caaaactgaa
      901 ggatttattt aatgtggatg cttttaagct ggaatcatta gaagcaaaaa acagagcatt
      961 gaatgaacag attgcaagat tggaacaaga aagagaaaaa gaaccgaatc gtctagagtc
     1021 gttgagaaaa ctgaaggctt ccttacaagg agatgttcaa aagtatcagg catacatgag
     1081 caatttggag tctcattcag ccattcttga ccagaaatta aatggtctca atgaggaaat
     1141 tgctagagta gaactagaat gtgaaacaat aaaacaggag aacactcgac tacagaatat
     1201 cattgacaac cagaagtact cagttgcaga cattgagcga ataaatcatg aaagaaatga
     1261 attgcagcag actattaata aattaaccaa ggacctggaa gctgaacaac agaagttgtg
     1321 gaatgaggag ttaaaatatg ccagaggcaa agaagcgatt gaaacacaat tagcagagta
     1381 tcacaaattg gctagaaaat taaaacttat tcctaaaggt gctgagaatt ccaaaggtta
     1441 tgactttgaa attaagttta atcccgaggc tggtgccaac tgccttgtca aatacagggc
     1501 tcaagtttat gtacctctta aggaactcct gaatgaaact gaagaagaaa ttaataaagc
     1561 cctaaataaa aaaatgggtt tggaggatac tttagaacaa ttgaatgcaa tgataacaga
     1621 aagcaagaga agtgtgagaa ctctgaaaga agaagttcaa aagctggatg atctttacca
     1681 acaaaaaatt aaggaagcag aggaagagga tgaaaaatgt gccagtgagc ttgagtcctt
     1741 ggagaaacac aagcacctgc tagaaagtac tgttaaccag gggctcagtg aagctatgaa
     1801 tgaattagat gctgttcagc gggaatacca actagttgtg caaaccacga ctgaagaaag
     1861 acgaaaagtg ggaaataact tgcaacgtct gttagagatg gttgctacac atgttgggtc
     1921 tgtagagaaa catcttgagg agcagattgc taaagttgat agagaatatg aagaatgcat
     1981 gtcagaagat ctctcggaaa atattaaaga gattagagat aagtatgaga agaaagctac
     2041 tctaattaag tcttctgaag aatgaagata aaatgttgat catgtatata tatccatagt
     2101 gaataaaatt gtctcagtaa agtgta
//
