LOCUS       NM_014286               5164 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens neuronal calcium sensor 1 (NCS1), transcript variant
            1, mRNA.
ACCESSION   NM_014286
VERSION     NM_014286.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Sanchez,J.C., Aleman,A., Henao,J.F., Olaya,J.C. and Ehrlich,B.E.
  TITLE     NCS-1 protein regulates TRPA1 channel through the PI3K pathway in
            breast cancer and neuronal cells
  JOURNAL   J Physiol Biochem 80 (2), 451-463 (2024)
   PUBMED   38564162
  REMARK    GeneRIF: NCS-1 protein regulates TRPA1 channel through the PI3K
            pathway in breast cancer and neuronal cells.
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Baksheeva,V.E., Zamyatnin,A.A. Jr. and Zernii,E.Y.
  TITLE     [Neuronal Calcium Sensor-1: A Zinc/Redox-Dependent Protein of
            Nervous System Signaling Pathways]
  JOURNAL   Mol Biol (Mosk) 57 (6), 1098-1129 (2023)
   PUBMED   38062964
  REMARK    GeneRIF: [Neuronal Calcium Sensor-1: A Zinc/Redox-Dependent Protein
            of Nervous System Signaling Pathways].
            Review article
REFERENCE   3  (bases 1 to 5164)
  AUTHORS   Baksheeva,V.E., Baldin,A.V., Zalevsky,A.O., Nazipova,A.A.,
            Kazakov,A.S., Vladimirov,V.I., Gorokhovets,N.V., Devred,F.,
            Philippov,P.P., Bazhin,A.V., Golovin,A.V., Zamyatnin,A.A. Jr.,
            Zinchenko,D.V., Tsvetkov,P.O., Permyakov,S.E. and Zernii,E.Y.
  TITLE     Disulfide Dimerization of Neuronal Calcium Sensor-1: Implications
            for Zinc and Redox Signaling
  JOURNAL   Int J Mol Sci 22 (22), 12602 (2021)
   PUBMED   34830487
  REMARK    GeneRIF: Disulfide Dimerization of Neuronal Calcium Sensor-1:
            Implications for Zinc and Redox Signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5164)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5164)
  AUTHORS   Staby,L., Kemplen,K.R., Stein,A., Ploug,M., Clarke,J., Skriver,K.,
            Heidarsson,P.O. and Kragelund,B.B.
  TITLE     Disorder in a two-domain neuronal Ca2+-binding protein regulates
            domain stability and dynamics using ligand mimicry
  JOURNAL   Cell Mol Life Sci 78 (5), 2263-2278 (2021)
   PUBMED   32936312
  REMARK    GeneRIF: Disorder in a two-domain neuronal Ca(2+)-binding protein
            regulates domain stability and dynamics using ligand mimicry.
REFERENCE   6  (bases 1 to 5164)
  AUTHORS   Chen,C., Yu,L., Zhang,P., Jiang,J., Zhang,Y., Chen,X., Wu,Q., Wu,Q.
            and Zhao,S.
  TITLE     Human neuronal calcium sensor-1 shows the highest expression level
            in cerebral cortex
  JOURNAL   Neurosci Lett 319 (2), 67-70 (2002)
   PUBMED   11825672
REFERENCE   7  (bases 1 to 5164)
  AUTHORS   Zhao,X., Varnai,P., Tuymetova,G., Balla,A., Toth,Z.E.,
            Oker-Blom,C., Roder,J., Jeromin,A. and Balla,T.
  TITLE     Interaction of neuronal calcium sensor-1 (NCS-1) with
            phosphatidylinositol 4-kinase beta stimulates lipid kinase activity
            and affects membrane trafficking in COS-7 cells
  JOURNAL   J Biol Chem 276 (43), 40183-40189 (2001)
   PUBMED   11526106
REFERENCE   8  (bases 1 to 5164)
  AUTHORS   Nakamura,T.Y., Pountney,D.J., Ozaita,A., Nandi,S., Ueda,S., Rudy,B.
            and Coetzee,W.A.
  TITLE     A role for frequenin, a Ca2+-binding protein, as a regulator of Kv4
            K+-currents
  JOURNAL   Proc Natl Acad Sci U S A 98 (22), 12808-12813 (2001)
   PUBMED   11606724
REFERENCE   9  (bases 1 to 5164)
  AUTHORS   Bourne,Y., Dannenberg,J., Pollmann,V., Marchot,P. and Pongs,O.
  TITLE     Immunocytochemical localization and crystal structure of human
            frequenin (neuronal calcium sensor 1)
  JOURNAL   J Biol Chem 276 (15), 11949-11955 (2001)
   PUBMED   11092894
REFERENCE   10 (bases 1 to 5164)
  AUTHORS   Burgoyne,R.D. and Weiss,J.L.
  TITLE     The neuronal calcium sensor family of Ca2+-binding proteins
  JOURNAL   Biochem J 353 (Pt 1), 1-12 (2001)
   PUBMED   11115393
  REMARK    Review article
            Erratum:[Biochem J 2001 Mar 15;354(Pt 3):727]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL360004.22, BC004856.2,
            AF134479.1, AL161981.1, BM011362.1, BU150740.1, AI564128.1,
            AI521726.1, CF453730.1, AI768193.1 and CK301145.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014286.3.
            
            Summary: This gene is a member of the neuronal calcium sensor gene
            family, which encode calcium-binding proteins expressed
            predominantly in neurons. The protein encoded by this gene
            regulates G protein-coupled receptor phosphorylation in a
            calcium-dependent manner and can substitute for calmodulin. The
            protein is associated with secretory granules and modulates
            synaptic transmission and synaptic plasticity. Multiple transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF134479.1, SRR1660807.72178.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372398.6/ ENSP00000361475.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-174               AL360004.22        43834-44007
            175-775             BC004856.2         1-601
            776-1274            AF134479.1         583-1081
            1275-2038           AL161981.1         394-1157
            2039-3356           AF134479.1         1846-3163
            3357-3783           BM011362.1         300-726
            3784-4138           BU150740.1         300-654
            4139-4425           AI564128.1         62-348              c
            4426-4488           AI521726.1         4-66                c
            4489-4991           CF453730.1         275-777
            4992-5149           AI768193.1         12-169              c
            5150-5164           CK301145.1         20-34               c
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..5164
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /note="neuronal calcium sensor 1"
                     /db_xref="GeneID:23413"
                     /db_xref="HGNC:HGNC:3953"
                     /db_xref="MIM:603315"
     exon            1..324
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..833
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /note="isoform 1 is encoded by transcript variant 1;
                     frequenin-like ubiquitous protein; frequenin-like protein;
                     frequenin homolog"
                     /codon_start=1
                     /product="neuronal calcium sensor 1 isoform 1"
                     /protein_id="NP_055101.2"
                     /db_xref="CCDS:CCDS6932.1"
                     /db_xref="GeneID:23413"
                     /db_xref="HGNC:HGNC:3953"
                     /db_xref="MIM:603315"
                     /translation="MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLD
                     AAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLR
                     WAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNA
                     DGKLTLQEFQEGSKADPSIVQALSLYDGLV"
     exon            325..349
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     exon            350..488
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     exon            489..567
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     exon            568..656
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     exon            657..734
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     exon            735..850
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     exon            851..5164
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4465..4470
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /note="hexamer: AATAAA"
     polyA_site      4488
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /note="major polyA site"
     regulatory      5135..5140
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
                     /note="hexamer: ATTAAA"
     polyA_site      5164
                     /gene="NCS1"
                     /gene_synonym="FLUP; FREQ"
ORIGIN      
        1 agtaaccagg gacgaccgcg gccacaccgc gccggcgccg gcgcccagcc caggcagccc
       61 cgcgccgcgg cgcccggacc gcccgggcct gcccagcggc cgccccacgc cccgggcgcc
      121 ccggcgccga cagccgcgca gcgcagcgcg ggcgccgcag acaaaggcgc ggccccggcc
      181 cggcccgccc ggcccagccg ctcctgctgg gcgccccaac cgggtccggc ccgggggggc
      241 gggggccgcg gccgccgagg atggggaaat ccaacagcaa gttgaagccc gaagttgtgg
      301 aggagctgac caggaagacc tactttaccg agaaggaggt ccagcagtgg tacaaaggct
      361 tcatcaagga ctgccccagt gggcagctgg atgcggcagg cttccagaag atctacaagc
      421 aattcttccc gttcggagac cccaccaagt ttgccacatt tgttttcaac gtctttgatg
      481 aaaacaagga cgggcgaatt gagttctccg agttcatcca ggcgctgtcg gtgacctcac
      541 ggggaaccct ggatgagaag ctacggtggg ccttcaagct ctacgacttg gacaatgatg
      601 gctacatcac caggaatgag atgctggaca ttgtggatgc catttaccag atggtgggga
      661 ataccgtgga gctcccagag gaggagaaca ctcctgagaa gagggtggac cggatctttg
      721 ccatgatgga taagaatgcc gacgggaagc tgaccctgca ggagttccag gagggttcca
      781 aggcagaccc gtccattgtg caggcgctgt ccctctacga cgggctggta tagtcccagg
      841 ctggagctgg atgcctggga accactcacc tccttctgtg ccatgaggcc acctcagccc
      901 tgacaccaac cccgtgcgtc cacccagcct tcttccgcat ccacacacag ccggctgccc
      961 ttgacccggg aggccccggc tctcctctcc cctgtcctgc acccatcccc cgcctgaagc
     1021 caccggctcc aattgccagc aacctctgct tgtccggaaa acgacaacac gaaatggaaa
     1081 aggctacagc cctctgcata aaccaaggac ttggctgcct cgcaggcagc ctccgttcct
     1141 cccgctctct tgcgcgtgtg cttttgtttt ttattttgaa cagacgtttt aaaagaaaaa
     1201 aaaacaacta ccttctgtcc tagaagacac agactgacag atggggtgaa ggcctgggga
     1261 cctcagagaa ctctgccttg ccctcgtccc tcgtccttcg gcagccggag aggctgtggg
     1321 tgggccgagg gtgtctaggg gttctgcctg gtcaacgtta tttgtcgtcc catcttttgg
     1381 cagcaaaacc acctgcgtgg ctaggatgat taattatgag gatgatgatt ttttttgtga
     1441 taacagtatt gtgctttttg tggggaaagt gaggtttttt tttatataca tatataattg
     1501 atatctttaa tttattggtt gttaactgtt gctgctgcct ggtgtgtcct cagctcccag
     1561 ggctgcgggc ccaccgttta catgtgcacg ccctgaccca cctgcccacg ccgacttggg
     1621 aggatggtgg cctgcagcgg ccaagaagcc aaaaaaaatt tttttttttt cagatactgt
     1681 gcttgatttt tggagagggg agaggtggaa attcctaaat ggctaatgca ctgttccctc
     1741 cagcccgaat gcctcctgcc aaaccccttt tccctgctgc ctctgtcccc gcatccttgt
     1801 tctcccctgg gtccgtaaca ttttttccga ggatgaacag gggacatctt taggtttctc
     1861 aactcttgct ttggtgtttg ccgcagcatg gaaaacaggg cgcctaaggc tgggagctgg
     1921 aagaaggggc attgggtacc caggcagagt caggagaggt ggtctttgaa gtaagttagc
     1981 agaaatcaag gggacccccg cctccttggg ctggggaggg gatttcaaga tagttcatga
     2041 ctctctcccg ctctgccttc cctccttcct atctgctttt tccagtaaac tgcatggtgt
     2101 ccttccctgg ccttctcttg gctcaaaggc tgggagggag ggaaggagag aagagttcca
     2161 ggcaatccca tcaatatagt ccctacacct ggggctgcgg cccacatgtc ttcacggagg
     2221 cttccagcgg tgcctgccac tgaggcaggt gcggccccag gaccatcacc aggaatgcga
     2281 ggccaccctg gaccagaggt aggagcccaa ggtccggccc ttgctctttg attgtgggca
     2341 gcctcctgcc ctctctgggt ctcagttgcc ccatctgcag agcgaggagg cccgggctgg
     2401 ttggtcttga aggccctttt ccatgccgac atcatgtcac tctaggcctg gggttcagtt
     2461 tcctgtggct ggtgatgctg tggttaagtt tgcttgaccc cagcagcccg agggactgtc
     2521 tgagtcacag cacagcccct attgcgtggc tgctggtgtg tggggtcagt tccagcagat
     2581 gaatgtgtca tgtggcacac cttgtccctt cccgcagcat ttcctggttc cccccagacc
     2641 cttgagcgct ctttgggacc cagaaggagt ccttgcacag ggaaggcttg aggtgagaag
     2701 ccgcttccca gactgtcagg gccaggcctg ggtctagaat tcttgctgct gctttgcaga
     2761 gtcaacagcc catcagccca tgttttagag gggacacttt ggtcctcggt tcccaccctc
     2821 agcaagcagg cctccagccc gaggaaggcc tctgccgtag tgacgttgcc gtgtggggct
     2881 gcgtggctgt tccccttggc tggagcattc agccaacccc agcgtccccc ctgaggcgtt
     2941 cattggcagc cccctaggac tgcacgctgg ccccacggta accccccctc ccccaccaac
     3001 atcctgcagg gatggggtca gtggttccac cttcacaggc cactttgaag ggtggattct
     3061 ttgaggcccc tgccagtcgg ctccctgctc agctgctggc ccgggcgacc tgggactcag
     3121 caccaacggc tgaagtttct cagctgggct ctgacctggg gtctggggca gggaacgaac
     3181 atggtggctt tgggctgaga ggatgaggga ggtctttccc aggtcaaatt actttccttt
     3241 ggcctctgcc tgaggctcga tttgcctctc tggtccaatg ggactgacac tgttgtacaa
     3301 cctgacctgt ggctgagggt gtctgggctt aagcatgtgg accccttcgg tgtgtccggc
     3361 cttcctccat cgtcctgccc tttggccttt tggtttgaag ccacaggtgt ggcttctggc
     3421 cttagcagat ggtatgcttg cggaccgcag cccagcatgc cggtgggccc acagcccgag
     3481 ccagcccaga gctgccggaa gggccgccct tcccggccct ggcggggtgc tggacactgg
     3541 ccattttcac tagagtttgc ctggcaggga ccgatctctg ccccctcctc tccccaggcc
     3601 tctggctgca gtgatgccgc agaatcctga gccaggtgcc tcctgagcag cccgtgcgcc
     3661 tctccacagc ggcgtttgcc acccaatgcg gctcgcttca gatgctctga tgcagagggc
     3721 acgcccatag tccctctgca gagcctcgca ctggggccag ggcaggcacc agccccaggc
     3781 ggccagtcgg ccacggcctg tcctcttcct cgtagcgtct gctcctcact ttgtgttgat
     3841 ggtgacttag gagaatgttc cgattttcca tgatctaagc aggccacgtt taaaataaca
     3901 tcaaggcaag cgtacgtgtc accctctgta ctgacatctc ttcccctgaa atgcttttca
     3961 gtttgacagc ccgtttccta gacaagtgca cctggggttt caggaacttt gtgttttttc
     4021 ggagggggtt ggtggggagg tcgggatgcc tgggatccct tcctggagag gcaggctgtc
     4081 tctggaaaaa gcctccattg cccacccgcc aggcggaaag tcaccctgtt cccagcgcgg
     4141 tttcagcatt taattttaag ggagctaagg aagcgcggcg cgccccctgg tggtggtaag
     4201 ccgccaacgc acctgggggc tgcaacccca ccggacgggt ggtccggagg gaggctggag
     4261 cggggaggcg aggagggggc tgtgagtcct cagaggccct gggccaccac atttctggca
     4321 gcgtttccca gacacccctc tgctaggcca tccctggata gcaagtgaat taacttaagg
     4381 gcactgtgat gggaagcctt gcccccctct tttttttttt tttttaatat ctgcggaata
     4441 aacccaatgg ttaatttttg aatgaataaa aggcttttgt tgaataaaca gctggtccca
     4501 tcttctgtct tggcatctta gcatccaggc tcaggctctg ccccttctcc aggatggggt
     4561 agccccgagt cgccctcccc agtctgcaca ttccctgttg tccctgttcc tgcagtggct
     4621 cccggcccca gggaggccca cctcactccc agcctgactc ggtggctggc ttccttcagg
     4681 actttgcgca agtcagttct gctcattggg tctcaatttc cccatccctt ggatgggagc
     4741 aagagtctct gctgggcctg cctcgcaggg gcctggtgag acccaaatgt gagtgtcatc
     4801 atcaaagccc ctcacagaag tggaggacgg tgcccaagag tagcggtttg gactgctgct
     4861 gcctcccacc cggagcctgc cacttggggg agaaattggt ataatgcttg caaaaacaaa
     4921 caaacaaaag gcaatgtctt ctggttgtgg ttatttcctt tcctgcttgc ctccccagcc
     4981 ccctttgagt ctctttttgg ggtgccgtcc tgtctgaacc tgccggtgtg tgtctctggg
     5041 gccagggtca gggcgaggcc caggggtgga caggggccgt gtagcatgcc ccagcctccc
     5101 caagctcctg ctgtatgtcg tccatgtcac gccaattaaa cacgcttcct ggacttgtcc
     5161 tcgc
//
