LOCUS       NM_006540               8430 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens nuclear receptor coactivator 2 (NCOA2), transcript
            variant 2, mRNA.
ACCESSION   NM_006540
VERSION     NM_006540.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Yu,X., Wang,X., Ma,K., Gao,D., Deng,Y., Zhou,D., Ding,W., Zhao,Y.,
            Liu,Q. and Zhou,Z.
  TITLE     Tai/NCOA2 suppresses the Hedgehog pathway by directly targeting the
            transcription factor Ci/GLI
  JOURNAL   Proc Natl Acad Sci U S A 121 (47), e2409380121 (2024)
   PUBMED   39531503
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Warmke,L.M., Mustafa,A., Zou,Y.S., Davis,J.L., Ulbright,T.M. and
            Segura,S.E.
  TITLE     Myxoid epithelioid smooth muscle tumor of the vulva: A distinct
            entity with MEF2D::NCOA2 gene fusion
  JOURNAL   Genes Chromosomes Cancer 63 (1), e23209 (2024)
   PUBMED   37870842
  REMARK    GeneRIF: Myxoid epithelioid smooth muscle tumor of the vulva: A
            distinct entity with MEF2D::NCOA2 gene fusion.
REFERENCE   3  (bases 1 to 8430)
  AUTHORS   Watson,S., LaVigne,C.A., Xu,L., Surdez,D., Cyrta,J., Calderon,D.,
            Cannon,M.V., Kent,M.R., Silvius,K.M., Kucinski,J.P., Harrison,E.N.,
            Murchison,W., Rakheja,D., Tirode,F., Delattre,O., Amatruda,J.F. and
            Kendall,G.C.
  TITLE     VGLL2-NCOA2 leverages developmental programs for pediatric
            sarcomagenesis
  JOURNAL   Cell Rep 42 (1), 112013 (2023)
   PUBMED   36656711
  REMARK    GeneRIF: VGLL2-NCOA2 leverages developmental programs for pediatric
            sarcomagenesis.
REFERENCE   4  (bases 1 to 8430)
  AUTHORS   Wang,L., Motoi,T., Khanin,R., Olshen,A., Mertens,F., Bridge,J., Dal
            Cin,P., Antonescu,C.R., Singer,S., Hameed,M., Bovee,J.V.,
            Hogendoorn,P.C., Socci,N. and Ladanyi,M.
  TITLE     Identification of a novel, recurrent HEY1-NCOA2 fusion in
            mesenchymal chondrosarcoma based on a genome-wide screen of
            exon-level expression data
  JOURNAL   Genes Chromosomes Cancer 51 (2), 127-139 (2012)
   PUBMED   22034177
  REMARK    GeneRIF: The novel HEY1-NCOA2 fusion appears to be the defining and
            diagnostic gene fusion in mesenchymal chondrosarcomas
REFERENCE   5  (bases 1 to 8430)
  AUTHORS   Strehl,S., Nebral,K., Konig,M., Harbott,J., Strobl,H., Ratei,R.,
            Struski,S., Bielorai,B., Lessard,M., Zimmermann,M., Haas,O.A. and
            Izraeli,S.
  TITLE     ETV6-NCOA2: a novel fusion gene in acute leukemia associated with
            coexpression of T-lymphoid and myeloid markers and frequent NOTCH1
            mutations
  JOURNAL   Clin Cancer Res 14 (4), 977-983 (2008)
   PUBMED   18281529
  REMARK    GeneRIF: ETV6-NCOA2 fusion may define a novel subgroup of acute
            leukemia with T-lymphoid and myeloid features, which is associated
            with a high prevalence of NOTCH1 mutations.
REFERENCE   6  (bases 1 to 8430)
  AUTHORS   Fryer,C.J. and Archer,T.K.
  TITLE     Chromatin remodelling by the glucocorticoid receptor requires the
            BRG1 complex
  JOURNAL   Nature 393 (6680), 88-91 (1998)
   PUBMED   9590696
REFERENCE   7  (bases 1 to 8430)
  AUTHORS   Carapeti,M., Aguiar,R.C., Goldman,J.M. and Cross,N.C.
  TITLE     A novel fusion between MOZ and the nuclear receptor coactivator
            TIF2 in acute myeloid leukemia
  JOURNAL   Blood 91 (9), 3127-3133 (1998)
   PUBMED   9558366
REFERENCE   8  (bases 1 to 8430)
  AUTHORS   Voegel,J.J., Heine,M.J., Tini,M., Vivat,V., Chambon,P. and
            Gronemeyer,H.
  TITLE     The coactivator TIF2 contains three nuclear receptor-binding motifs
            and mediates transactivation through CBP binding-dependent and
            -independent pathways
  JOURNAL   EMBO J 17 (2), 507-519 (1998)
   PUBMED   9430642
REFERENCE   9  (bases 1 to 8430)
  AUTHORS   Hong,H., Kohli,K., Garabedian,M.J. and Stallcup,M.R.
  TITLE     GRIP1, a transcriptional coactivator for the AF-2 transactivation
            domain of steroid, thyroid, retinoid, and vitamin D receptors
  JOURNAL   Mol Cell Biol 17 (5), 2735-2744 (1997)
   PUBMED   9111344
REFERENCE   10 (bases 1 to 8430)
  AUTHORS   Voegel,J.J., Heine,M.J., Zechel,C., Chambon,P. and Gronemeyer,H.
  TITLE     TIF2, a 160 kDa transcriptional mediator for the ligand-dependent
            activation function AF-2 of nuclear receptors
  JOURNAL   EMBO J 15 (14), 3667-3675 (1996)
   PUBMED   8670870
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL712418.1, X97674.1,
            AW504723.1, DA691975.1, CN291204.1, CB270668.1, CK903771.1 and
            BC047111.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006540.3.
            
            Summary: The protein encoded by this gene functions as a
            transcriptional coactivator for nuclear hormone receptors,
            including steroid, thyroid, retinoid, and vitamin D receptors. The
            encoded protein acts as an intermediary factor for the
            ligand-dependent activity of these nuclear receptors, which
            regulate their target genes upon binding of cognate response
            elements. This gene has been found to be involved in translocations
            that result in fusions with other genes in various cancers,
            including the lysine acetyltransferase 6A (KAT6A) gene in acute
            myeloid leukemia, the ETS variant 6 (ETV6) gene in acute
            lymphoblastic leukemia, and the hes related family bHLH
            transcription factor with YRPW motif 1 (HEY1) gene in mesenchymal
            chondrosarcoma. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 all encode isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X97674.1, SRR11853566.26632.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000452400.7/ ENSP00000399968.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AL712418.1         33-55
            24-4813             X97674.1           1-4790
            4814-5223           AW504723.1         31-440
            5224-5688           X97674.1           5201-5665
            5689-6248           DA691975.1         13-572
            6249-6865           CN291204.1         34-650
            6866-7184           CB270668.1         173-491
            7185-7556           CK903771.1         208-579
            7557-8430           BC047111.1         930-1803
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q13.3"
     gene            1..8430
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /note="nuclear receptor coactivator 2"
                     /db_xref="GeneID:10499"
                     /db_xref="HGNC:HGNC:7669"
                     /db_xref="MIM:601993"
     exon            1..109
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            110..166
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            167..271
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..4580
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /note="isoform a is encoded by transcript variant 2;
                     glucocorticoid receptor-interacting protein-1;
                     transcriptional intermediary factor 2; class E basic
                     helix-loop-helix protein 75; p160 steroid receptor
                     coactivator 2"
                     /codon_start=1
                     /product="nuclear receptor coactivator 2 isoform a"
                     /protein_id="NP_006531.1"
                     /db_xref="CCDS:CCDS47872.1"
                     /db_xref="GeneID:10499"
                     /db_xref="HGNC:HGNC:7669"
                     /db_xref="MIM:601993"
                     /translation="MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENK
                     YIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDV
                     SSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVSENVTQYLRYNQEELMNKSVY
                     SILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDSEEEGHDNQ
                     EAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDL
                     QGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFS
                     QIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMG
                     KPLNPISSNSPAHQALCSGNPGQDMTLSSNINFPINGPKEQMGMPMGRFGGSGGMNHV
                     SGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRHRMSPGVAGSPRIPPSQFS
                     PAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMGNLQNSP
                     VNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSE
                     RADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTS
                     LKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKK
                     ENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQ
                     PGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAIINDLMQLTAENSPVTPV
                     GAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVI
                     PQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAM
                     NRPVQGGMIRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPP
                     NQTAPWPESILPIDQASFASQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALR
                     NFDGLEEIDRALGIPELVSQSQAVDPEQFSSQDSNIMLEQKAPVFPQQYASQAQMAQG
                     SYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLRLQLQHRLQAQQNRQ
                     PLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQRT
                     LMMRGQGLNMTPSMVAPSGMPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATT
                     PQSPLMSPRMAHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQ
                     ANTSMYSNNMNINVSMATNTGGMSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPA
                     LRGGNLFPNQLPGMDMIKQEGDTTRKYC"
     exon            272..444
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            445..548
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            549..726
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            727..915
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1017
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1161
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1309
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..2579
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2790
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2997
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3213
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3214..3343
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3509
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3510..3788
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3789..3866
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3867..4101
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4102..4279
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4280..4478
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4479..4568
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4569..8430
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8404..8409
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /note="hexamer: AATAAA"
     polyA_site      8430
                     /gene="NCOA2"
                     /gene_synonym="bHLHe75; GRIP1; KAT13C; NCoA-2; SRC2; TIF2"
                     /note="major polyA site"
ORIGIN      
        1 agcagttatt aacacgggaa catggcggcc gcagcctcgg ctacagcttc ggcggcgaag
       61 gtcagcgccg acggcagccg gcacctgacg gcgtgaccga cccgagccga tttctcttgg
      121 atttggctac acacttatag atcttctgca ctgtttacag gcacagttgc tgatatgtgt
      181 tcaagatgag tgggatggga gaaaatacct ctgacccctc cagggcagag acaagaaagc
      241 gcaaggaatg tcctgaccaa cttggaccca gccccaaaag gaacactgaa aaacgtaatc
      301 gtgaacagga aaataaatat atagaagaac ttgcagagtt gatttttgca aattttaatg
      361 atatagacaa ctttaacttc aaacctgaca aatgtgcaat cttaaaagaa actgtgaagc
      421 aaattcgtca gatcaaagaa caagagaaag cagcagctgc caacatagat gaagtgcaga
      481 agtcagatgt atcctctaca gggcagggtg tcatcgacaa ggatgcgctg gggcctatga
      541 tgcttgaggc ccttgatggg ttcttctttg tagtgaacct ggaaggcaac gttgtgtttg
      601 tgtcagagaa tgtgacacag tatctaaggt ataaccaaga agagctgatg aacaaaagtg
      661 tatatagcat cttgcatgtt ggggaccaca cggaatttgt caaaaacctg ctgccaaagt
      721 ctatagtaaa tgggggatct tggtctggcg aacctccgag gcggaacagc cataccttca
      781 attgtcggat gctggtaaaa cctttacctg attcagaaga ggagggtcat gataaccagg
      841 aagctcatca gaaatatgaa actatgcagt gcttcgctgt ctctcaacca aagtccatca
      901 aagaagaagg agaagatttg cagtcctgct tgatttgcgt ggcaagaaga gttcccatga
      961 aggaaagacc agttcttccc tcatcagaaa gttttactac tcgccaggat ctccaaggca
     1021 agatcacgtc tctggatacc agcaccatga gagcagccat gaaaccaggc tgggaggacc
     1081 tggtaagaag gtgtattcag aagttccatg cgcagcatga aggagaatct gtgtcctatg
     1141 ctaagaggca tcatcatgaa gtactgagac aaggattggc attcagtcaa atctatcgtt
     1201 tttccttgtc tgatggcact cttgttgctg cacaaacgaa gagcaaactc atccgttctc
     1261 agactactaa tgaacctcaa cttgtaatat ctttacatat gcttcacaga gagcagaatg
     1321 tgtgtgtgat gaatccggat ctgactggac aaacgatggg gaagccactg aatccaatta
     1381 gctctaacag ccctgcccat caggccctgt gcagtgggaa cccaggtcag gacatgaccc
     1441 tcagtagcaa tataaatttt cccataaatg gcccaaagga acaaatgggc atgcccatgg
     1501 gcaggtttgg tggttctggg ggaatgaacc atgtgtcagg catgcaagca accactcctc
     1561 agggtagtaa ctatgcactc aaaatgaaca gcccctcaca aagcagccct ggcatgaatc
     1621 caggacagcc cacctccatg ctttcaccaa ggcatcgcat gagccctgga gtggctggca
     1681 gccctcgaat cccacccagt cagttttccc ctgcaggaag cttgcattcc cctgtgggag
     1741 tttgcagcag cacaggaaat agccatagtt ataccaacag ctccctcaat gcacttcagg
     1801 ccctcagcga ggggcacggg gtctcattag ggtcatcgtt ggcttcacca gacctaaaaa
     1861 tgggcaattt gcaaaactcc ccagttaata tgaatcctcc cccactcagc aagatgggaa
     1921 gcttggactc aaaagactgt tttggactat atggggagcc ctctgaaggt acaactggac
     1981 aagcagagag cagctgccat cctggagagc aaaaggaaac aaatgacccc aacctgcccc
     2041 cggccgtgag cagtgagaga gctgacgggc agagcagact gcatgacagc aaagggcaga
     2101 ccaaactcct gcagctgctg accaccaaat ctgatcagat ggagccctcg cccttagcca
     2161 gctctttgtc ggatacaaac aaagactcca caggtagctt gcctggttct gggtctacac
     2221 atggaacctc gctcaaggag aagcataaaa ttttgcacag actcttgcag gacagcagtt
     2281 cccctgtgga cttggccaag ttaacagcag aagccacagg caaagacctg agccaggagt
     2341 ccagcagcac agctcctgga tcagaagtga ctattaaaca agagccggtg agccccaaga
     2401 agaaagagaa tgcactactt cgctatttgc tagataaaga tgatactaaa gatattggtt
     2461 taccagaaat aacccccaaa cttgagagac tggacagtaa gacagatcct gccagtaaca
     2521 caaaattaat agcaatgaaa actgagaagg aggagatgag ctttgagcct ggtgaccagc
     2581 ctggcagtga gctggacaac ttggaggaga ttttggatga tttgcagaat agtcaattac
     2641 cacagctttt cccagacacg aggccaggcg cccctgctgg atcagttgac aagcaagcca
     2701 tcatcaatga cctcatgcaa ctcacagctg aaaacagccc tgtcacacct gttggagccc
     2761 agaaaacagc actgcgaatt tcacagagca cttttaataa cccacgacca gggcaactgg
     2821 gcaggttatt gccaaaccag aatttaccac ttgacatcac attgcaaagc ccaactggtg
     2881 ctggaccttt cccaccaatc agaaacagta gtccctactc agtgatacct cagccaggaa
     2941 tgatgggtaa tcaagggatg ataggaaacc aaggaaattt agggaacagt agcacaggaa
     3001 tgattggtaa cagtgcttct cggcctacta tgccatctgg agaatgggca ccgcagagtt
     3061 cggctgtgag agtcacctgt gctgctacca ccagtgccat gaaccggcca gtccaaggag
     3121 gtatgattcg gaacccagca gccagcatcc ccatgaggcc cagcagccag cctggccaaa
     3181 gacagacgct tcagtctcag gtcatgaata tagggccatc tgaattagag atgaacatgg
     3241 ggggacctca gtatagccaa caacaagctc ctccaaatca gactgcccca tggcctgaaa
     3301 gcatcctgcc tatagaccag gcgtcttttg ccagccaaaa caggcagcca tttggcagtt
     3361 ctccagatga cttgctatgt ccacatcctg cagctgagtc tccgagtgat gagggagctc
     3421 tcctggacca gctgtatctg gccttgcgga attttgatgg cctggaggag attgatagag
     3481 ccttaggaat acccgaactg gtcagccaga gccaagcagt agatccagaa cagttctcaa
     3541 gtcaggattc caacatcatg ctggagcaga aggcgcccgt tttcccacag cagtatgcat
     3601 ctcaggcaca aatggcccag ggtagctatt ctcccatgca agatccaaac tttcacacca
     3661 tgggacagcg gcctagttat gccacactcc gtatgcagcc cagaccgggc ctcaggccca
     3721 cgggcctagt gcagaaccag ccaaatcaac taagacttca acttcagcat cgcctccaag
     3781 cacagcagaa tcgccagcca cttatgaatc aaatcagcaa tgtttccaat gtgaacttga
     3841 ctctgaggcc tggagtacca acacaggcac ctattaatgc acagatgctg gcccagagac
     3901 agagggaaat cctgaaccag catcttcgac agagacaaat gcatcagcaa cagcaagttc
     3961 agcaacgaac tttgatgatg agaggacaag ggttgaatat gacaccaagc atggtggctc
     4021 ctagtggtat gccagcaact atgagcaacc ctcggattcc ccaggcaaat gcacagcagt
     4081 ttccatttcc tccaaactac ggaataagtc agcaacctga tccaggcttt actggggcta
     4141 cgactcccca gagcccactt atgtcacccc gaatggcaca tacacagagt cccatgatgc
     4201 aacagtctca ggccaaccca gcctatcagg ccccctccga cataaatgga tgggcgcagg
     4261 ggaacatggg cggaaacagc atgttttccc agcagtcccc accacacttt gggcagcaag
     4321 caaacaccag catgtacagt aacaacatga acatcaatgt gtccatggcg accaacacag
     4381 gtggcatgag cagcatgaac cagatgacag gacagatcag catgacctca gtgacctccg
     4441 tgcctacgtc agggctgtcc tccatgggtc ccgagcaggt taatgatcct gctctgaggg
     4501 gaggcaacct gttcccaaac cagctgcctg gaatggatat gattaagcag gagggagaca
     4561 caacacggaa atattgctga cactgctgaa gccagttgct tcttcagctg accgggctca
     4621 cttgctcaaa acacttccag tctggagagc tgtgtctatt tgtttcaacc caactgacct
     4681 gccagccggt tctgctagag cagacaggcc tggccctggt tcccagggtg gcgtccactc
     4741 ggctgtggca ggaggagctg cctcttctct tgacagtctg aagctcgcat ccagacagtc
     4801 gctcagtctg ttcactgcat tcaccttagt gcaacttaga tctctcctgc aaaagtaaat
     4861 gttgacaggc aaatttcata cccatgtcag attgaatgta tttaaatgta tgtatttaag
     4921 gagaaccatg ctcttgttct gttcctgttc ggttccagac actggtttct tgctttgttt
     4981 tccctggcta acagtctagt gcaaaagatt aagattttat ctgggggaaa gaaaagaatt
     5041 ttttaaaaaa ttaaactaaa gatgttttaa gctaaagcct gaatttggga tggaagcagg
     5101 acagacaccg tggacagcgc tgtatttaca gacacaccca gtgcgtgaag accaacaaag
     5161 tcacagtcgt atctctagaa agctctaaag accatgttgg aaagagtctc cagttactga
     5221 acagatgaaa aggagcctgt gagagggctg ttaacattag caaatatttt ttccttgttt
     5281 tttctttgtt aaaaccaaac tggttcacct gaatcatgaa ttgagaagaa ataattttca
     5341 tttctaaatt aagtcccttt tagtttgatc agacagcttg aatcagcatc tcttcttccc
     5401 tgtcagcctg actcttccct tcccctctct cattccccat actccctatt ttcattcctt
     5461 ttttaaaaaa taatataagc tacagaaacc aggtaagccc tttatttcct taaatgtttt
     5521 gccagccact taccaattgc taagtattga atttcagaaa aaaaaaatgc atttactggc
     5581 aaggagaaga gcaaagttaa ggcttgatac caatcgagct aaggatacct gctttggaag
     5641 catgtttatt ctgttcccca gcaactctgg cctccaaaat gggagaaaac gccagtgtgt
     5701 ttaaattgat agcagatatc acgacagatt taacctctgc catgtgtttt ttattttgtt
     5761 ttttagcagt gctgactaag ccgaagtttt gtaaggtaca taaaatccaa tttatatgta
     5821 aacaagcaat aatttaagtt gagaacttat gtgttttaat tgtataattt ttgtgaggta
     5881 tacatattgt ggaattgact caaaaatgag gtacttcagt attaaattag atatcttcat
     5941 agcaatgtct cctaaaggtg ttttgtaaag gatatcaatg ccttgattag acctaatttg
     6001 tagacttaag actttttatt ttctaaacct tgtgattctg cttataagtc atttatctaa
     6061 tctatatgat atgcagccgc tgtaggaacc aattcttgat ttttatatgt ttatattctt
     6121 tcttaatgaa ccttagaaag actacatgtt actaagcagg ccacttttat ggttgttttt
     6181 cttgcccact ctggtagggg aaaaatcttt tattaatagg tgtcagtttc agacaactcc
     6241 aataccaaga aactgatgac cttttttgag ctatttctcc ttaaccccta atacctttct
     6301 tacctaaatt tcagaatgat atctttttga tgtctaaaaa gcaaagcatt ttataatatc
     6361 tcattagcca ggctttttag gaacagagaa gtttttcctt gaaatttgat tgtttgttta
     6421 tttgtggcag ggtgaaaaat gtacaaacta cccctattta tacttatatc tctctctata
     6481 tgtacgcatt atggaaatat aggaagagat atagcccaaa gatagatggt aagtagacag
     6541 tctgaaaatg ttgctccata ctaaagaacc tttctggaaa tttgcatttc gctacttttt
     6601 tccacttgag aggggtctca atacagggag gtggccaagg aacaacactt ctcagtggcc
     6661 accagttcat atatcccctc aaaaggctct acttttctcc accaaaccat tgggttgccc
     6721 aactgtgatg gaaaaaacaa gcagatgttg gcccagaact ccaggacctc cccgtgggct
     6781 gaggctcatg tggcatacaa ttcagtgtga ccagttttaa tgttcagaaa tatgtgaatt
     6841 agaatgactt ttaacttcac acagcacaaa agagaaatgg agtttaaact ggatttgttt
     6901 tgttttgagt ttcaagtccc ttttcactac aacaccgtcc aaaactatct acctatctct
     6961 tcttttttat ccatctgccc gctgtatttt aatgtcaatc ctaattcaga caaatccgca
     7021 acttcatgat actggtaatt tataataatt cgtgatcatt gctgttttga aggaaaagag
     7081 atgataaatt ctcttacggt ttcaatcagg taatggattt tgttttacat ctagttagga
     7141 gaagaaaagt aagtgaagga atcctctgat catattgatc actatagaag caaaagccaa
     7201 atcagttccc ttcattatca gtatttctca tgctgtacac aataaatttg ttagactgag
     7261 tacttggttt ttgtttaata gaacaaataa tcagtgtttc acttatatta ctaaatgtga
     7321 aaaacataat gtgaaaagta tacatacatt aacttggttg taaaactcat tttaagtgtt
     7381 catgtaatgt ggtaagttta aaaagtgaag tgagctgttt tactacaatg aattcgtttg
     7441 actcaaagat taaaatgccc ctccccctat gtgtatctct tatggcctgt aataattgaa
     7501 agcaatgttt ttgcagttta tataatgcaa tttttaatct gtgttcaaaa atggagttca
     7561 tatgggctta acacatgaaa tgggtggcac agatccactt gcagaaccca aagatacaca
     7621 atcagttttg aaatgcaact taagccatta attgttggtg tgaatttaaa attttctagt
     7681 gtttgtatgg tggtatgctg cctaagagca aagcatcctc tgcacaaaag aaaattactg
     7741 tagtggcttc gattttaatt agaattttct ccctggtgtt tctttataga ctaaagcaaa
     7801 atcttttaag tttcttacta caggtaaaag ctatgtgcaa gaacctcaaa atgctaaaat
     7861 ctagcataat gccttagatc tgtttttaag tcttgtatat tcctacatag ctgtccgatg
     7921 tattatattt gtatagtgaa ttgtgtacaa tttttgaata agtgcatcat cacttaacct
     7981 ttatgttgtt tgaatagtga tgatgataca tttcttcaga catttaactg tcattttttt
     8041 cctttgtcat ttgtgagttt tatttgtaca gttcctcatg aagttgcatc tgagatgtgt
     8101 gtatatgaaa agattataca agggtaaaat taagcaatat attaactatg gttcttcagg
     8161 agaaagctta cagtgtttca ggttgtgatt tattttcaaa atggagttga ctctgaacat
     8221 cactttgttc acctgcattg atgtttgtat ttctagtgtg agcagtttat gcaaaggtag
     8281 atatattcag agaaatttta aatacattta tgcaagttaa tattgctttg ctgatgctat
     8341 ttgcttattt gatgttaaat aatgctattg taaataaaga attctgttgt tattgcatca
     8401 tttaataaat tttaatgcct tcgggtttta
//
