LOCUS       NM_005381               3924 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens nucleolin (NCL), mRNA.
ACCESSION   NM_005381 XM_002342275
VERSION     NM_005381.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Jiang,C., Huang,Y., Gui,H., Liu,X., Li,H., Han,M. and Huang,S.
  TITLE     TLR4 TIR domain and nucleolin GAR domain synergistically mediate
            RSV infection and induce neuronal inflammatory damage in SH-SY5Y
            cells
  JOURNAL   J Med Virol 96 (4), e29570 (2024)
   PUBMED   38558098
  REMARK    GeneRIF: TLR4 TIR domain and nucleolin GAR domain synergistically
            mediate RSV infection and induce neuronal inflammatory damage in
            SH-SY5Y cells.
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Sengar,A.S., Kumar,M., Rai,C., Chakraborti,S., Kumar,D., Kumar,P.,
            Mukherjee,S., Mondal,K., Stewart,A. and Maity,B.
  TITLE     RGS6 drives cardiomyocyte death following nucleolar stress by
            suppressing Nucleolin/miRNA-21
  JOURNAL   J Transl Med 22 (1), 204 (2024)
   PUBMED   38409136
  REMARK    GeneRIF: RGS6 drives cardiomyocyte death following nucleolar stress
            by suppressing Nucleolin/miRNA-21.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3924)
  AUTHORS   Peggion,C., Massimino,M.L., Pereira,D., Granuzzo,S., Righetto,F.,
            Bortolotto,R., Agostini,J., Sartori,G., Bertoli,A. and Lopreiato,R.
  TITLE     Structural Integrity of Nucleolin Is Required to Suppress
            TDP-43-Mediated Cytotoxicity in Yeast and Human Cell Models
  JOURNAL   Int J Mol Sci 24 (24), 17466 (2023)
   PUBMED   38139294
  REMARK    GeneRIF: Structural Integrity of Nucleolin Is Required to Suppress
            TDP-43-Mediated Cytotoxicity in Yeast and Human Cell Models.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3924)
  AUTHORS   Ezzatifar,F., Rafiei,A. and Jeddi-Tehrani,M.
  TITLE     Nucleolin; A tumor associated antigen as a potential lung cancer
            biomarker
  JOURNAL   Pathol Res Pract 240, 154160 (2022)
   PUBMED   36335647
  REMARK    GeneRIF: Nucleolin; A tumor associated antigen as a potential lung
            cancer biomarker.
            Review article
REFERENCE   5  (bases 1 to 3924)
  AUTHORS   Cangelosi,D., Brignole,C., Bensa,V., Tamma,R., Malaguti,F.,
            Carlini,B., Giusto,E., Calarco,E., Perri,P., Ribatti,D.,
            Fonseca,N.A., Moreira,J.N., Eva,A., Amoroso,L., Conte,M.,
            Garaventa,A., Sementa,A.R., Corrias,M.V., Ponzoni,M. and
            Pastorino,F.
  TITLE     Nucleolin expression has prognostic value in neuroblastoma patients
  JOURNAL   EBioMedicine 85, 104300 (2022)
   PUBMED   36209521
  REMARK    GeneRIF: Nucleolin expression has prognostic value in neuroblastoma
            patients.
REFERENCE   6  (bases 1 to 3924)
  AUTHORS   Tuteja,N., Huang,N.W., Skopac,D., Tuteja,R., Hrvatic,S., Zhang,J.,
            Pongor,S., Joseph,G., Faucher,C., Amalric,F. et al.
  TITLE     Human DNA helicase IV is nucleolin, an RNA helicase modulated by
            phosphorylation
  JOURNAL   Gene 160 (2), 143-148 (1995)
   PUBMED   7642087
REFERENCE   7  (bases 1 to 3924)
  AUTHORS   Pasternack,M.S., Bleier,K.J. and McInerney,T.N.
  TITLE     Granzyme A binding to target cell proteins. Granzyme A binds to and
            cleaves nucleolin in vitro
  JOURNAL   J Biol Chem 266 (22), 14703-14708 (1991)
   PUBMED   1860869
REFERENCE   8  (bases 1 to 3924)
  AUTHORS   Srivastava,M., McBride,O.W., Fleming,P.J., Pollard,H.B. and
            Burns,A.L.
  TITLE     Genomic organization and chromosomal localization of the human
            nucleolin gene
  JOURNAL   J Biol Chem 265 (25), 14922-14931 (1990)
   PUBMED   2394707
REFERENCE   9  (bases 1 to 3924)
  AUTHORS   Srivastava,M., Fleming,P.J., Pollard,H.B. and Burns,A.L.
  TITLE     Cloning and sequencing of the human nucleolin cDNA
  JOURNAL   FEBS Lett 250 (1), 99-105 (1989)
   PUBMED   2737305
REFERENCE   10 (bases 1 to 3924)
  AUTHORS   Erard,M.S., Belenguer,P., Caizergues-Ferrer,M., Pantaloni,A. and
            Amalric,F.
  TITLE     A major nucleolar protein, nucleolin, induces chromatin
            decondensation by binding to histone H1
  JOURNAL   Eur J Biochem 175 (3), 525-530 (1988)
   PUBMED   3409881
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM761058.1, AK001314.1,
            AK000221.1, BQ001029.1 and AC017104.8.
            
            On Nov 23, 2018 this sequence version replaced NM_005381.2.
            
            Summary: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is
            involved in the synthesis and maturation of ribosomes. It is
            located mainly in dense fibrillar regions of the nucleolus. Human
            NCL gene consists of 14 exons with 13 introns and spans
            approximately 11kb. The intron 11 of the NCL gene encodes a small
            nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.71306.1,
                                           SRR1803614.53299.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03267761, SAMN03267766
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000322723.9/ ENSP00000318195.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-682               BM761058.1         11-692
            683-1282            AK001314.1         85-684
            1283-1605           AK000221.1         1251-1573
            1606-2484           AK001314.1         1008-1886
            2485-2707           BQ001029.1         16-238              c
            2708-3924           AC017104.8         62058-63274         c
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.1"
     gene            1..3924
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /note="nucleolin"
                     /db_xref="GeneID:4691"
                     /db_xref="HGNC:HGNC:7667"
                     /db_xref="MIM:164035"
     exon            1..149
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..2264
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /codon_start=1
                     /product="nucleolin"
                     /protein_id="NP_005372.2"
                     /db_xref="CCDS:CCDS33397.1"
                     /db_xref="GeneID:4691"
                     /db_xref="HGNC:HGNC:7667"
                     /db_xref="MIM:164035"
                     /translation="MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGE
                     EVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAK
                     AVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDD
                     EDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKG
                     KKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEA
                     PGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKN
                     DLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDA
                     RTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQ
                     GTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEK
                     ATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNA
                     RSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAK
                     AAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRG
                     GFGGRGGFRGGRGGGGDHKPQGKKTKFE"
     exon            150..266
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            267..744
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            745..942
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1029
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1171
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1296
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1420
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1578
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1702
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1836
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1963
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2187
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..3924
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2683..2688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /note="hexamer: ATTAAA"
     polyA_site      2707
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /note="major polyA site"
     regulatory      3898..3903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
                     /note="hexamer: ATTAAA"
     polyA_site      3924
                     /gene="NCL"
                     /gene_synonym="C23; Nsr1"
ORIGIN      
        1 agtctcgagc tctcgctggc cttcgggtgt acgtgctccg ggatcttcag cacccgcggc
       61 cgccatcgcc gtcgcttggc ttcttctgga ctcatctgcg ccacttgtcc gcttcacact
      121 ccgccgccat catggtgaag ctcgcgaagg caggtaaaaa tcaaggtgac cccaagaaaa
      181 tggctcctcc tccaaaggag gtagaagaag atagtgaaga tgaggaaatg tcagaagatg
      241 aagaagatga tagcagtgga gaagaggtcg tcatacctca gaagaaaggc aagaaggctg
      301 ctgcaacctc agcaaagaag gtggtcgttt ccccaacaaa aaaggttgca gttgccacac
      361 cagccaagaa agcagctgtc actccaggca aaaaggcagc agcaacacct gccaagaaga
      421 cagttacacc agccaaagca gttaccacac ctggcaagaa gggagccaca ccaggcaaag
      481 cattggtagc aactcctggt aagaagggtg ctgccatccc agccaagggg gcaaagaatg
      541 gcaagaatgc caagaaggaa gacagtgatg aagaggagga tgatgacagt gaggaggatg
      601 aggaggatga cgaggacgag gatgaggatg aagatgaaat tgaaccagca gcgatgaaag
      661 cagcagctgc tgcccctgcc tcagaggatg aggacgatga ggatgacgaa gatgatgagg
      721 atgacgatga cgatgaggaa gatgactctg aagaagaagc tatggagact acaccagcca
      781 aaggaaagaa agctgcaaaa gttgttcctg tgaaagccaa gaacgtggct gaggatgaag
      841 atgaagaaga ggatgatgag gacgaggatg acgacgacga cgaagatgat gaagatgatg
      901 atgatgaaga tgatgaggag gaggaagaag aggaggagga agagcctgtc aaagaagcac
      961 ctggaaaacg aaagaaggaa atggccaaac agaaagcagc tcctgaagcc aagaaacaga
     1021 aagtggaagg cacagaaccg actacggctt tcaatctctt tgttggaaac ctaaacttta
     1081 acaaatctgc tcctgaatta aaaactggta tcagcgatgt ttttgctaaa aatgatcttg
     1141 ctgttgtgga tgtcagaatt ggtatgacta ggaaatttgg ttatgtggat tttgaatctg
     1201 ctgaagacct ggagaaagcg ttggaactca ctggtttgaa agtctttggc aatgaaatta
     1261 aactagagaa accaaaagga aaagacagta agaaagagcg agatgcgaga acacttttgg
     1321 ctaaaaatct cccttacaaa gtcactcagg atgaattgaa agaagtgttt gaagatgctg
     1381 cggagatcag attagtcagc aaggatggga aaagtaaagg gattgcttat attgaattta
     1441 agacagaagc tgatgcagag aaaacctttg aagaaaagca gggaacagag atcgatgggc
     1501 gatctatttc cctgtactat actggagaga aaggtcaaaa tcaagactat agaggtggaa
     1561 agaatagcac ttggagtggt gaatcaaaaa ctctggtttt aagcaacctc tcctacagtg
     1621 caacagaaga aactcttcag gaagtatttg agaaagcaac ttttatcaaa gtaccccaga
     1681 accaaaatgg caaatctaaa gggtatgcat ttatagagtt tgcttcattc gaagacgcta
     1741 aagaagcttt aaattcctgt aataaaaggg aaattgaggg cagagcaatc aggctggagt
     1801 tgcaaggacc caggggatca cctaatgcca gaagccagcc atccaaaact ctgtttgtca
     1861 aaggcctgtc tgaggatacc actgaagaga cattaaagga gtcatttgac ggctccgttc
     1921 gggcaaggat agttactgac cgggaaactg ggtcctccaa agggtttggt tttgtagact
     1981 tcaacagtga ggaggatgcc aaagctgcca aggaggccat ggaagacggt gaaattgatg
     2041 gaaataaagt taccttggac tgggccaaac ctaagggtga aggtggcttc gggggtcgtg
     2101 gtggaggcag aggcggcttt ggaggacgag gtggtggtag aggaggccga ggaggatttg
     2161 gtggcagagg ccggggaggc tttggagggc gaggaggctt ccgaggaggc agaggaggag
     2221 gaggtgacca caagccacaa ggaaagaaga cgaagtttga atagcttctg tccctctgct
     2281 ttcccttttc catttgaaag aaaggactct ggggttttta ctgttacctg atcaatgaca
     2341 gagccttctg aggacattcc aagacagtat acagtcctgt ggtctccttg gaaatccgtc
     2401 tagttaacat ttcaagggca ataccgtgtt ggttttgact ggatattcat ataaactttt
     2461 taaagagttg agtgatagag ctaaccctta tctgtaagtt ttgaatttat attgtttcat
     2521 cccatgtaca aaaccatttt ttcctacaaa tagtttgggt tttgttgttg tttctttttt
     2581 ttgttttgtt tttgtttttt ttttttttgc gttcgtgggg ttgtaaaaga aaagaaagca
     2641 gaatgtttta tcatggtttt tgcttcagcg gctttaggac aaattaaaag tcaactctgg
     2701 tgccagacgt gttacttcct aaagagtgtt tcccctggaa tgtcactgga gagcatggca
     2761 aagccagctc tgccacttgc ttcacccatc ccaatggaaa tggcttagtg cgtgtttcca
     2821 gtatcccagc cctaactaac ttggttgaaa tgctggtgag gggacctgct cctgcagccc
     2881 tggtgctgac ttgaaggctg ctgcagcttc tcctactttt agcaggtctg aggattatgt
     2941 cctgaagacc actctggaaa gaggtgcagg aacagattag tcaggtttcc taggacaagg
     3001 aagagcttca gggaagagca gtggctaact cctgtaatcc caacacttgg ggaggccgag
     3061 gcaggcagat caactgaggt caggagttga agaccagcct ggccaacatg gtgaaagccc
     3121 atctctacta aaaatacaaa aattagctgg gcatggtggt gtactcctgt agtcccagct
     3181 actcaggagg ctgaagcggg agagtcacgt gaacccggga agcagagtga gctgagcaca
     3241 cactactata ctccaggctg ggtaacaaag cgagactccc atctcccaaa aagcagttct
     3301 ggaatagaac tcacgctaga tggatagacc agtggacact ttggaacctt ggggctgggg
     3361 aggaaactgc ccatccagta aacccccaaa aagccatttg ttctgcacta cgtatattgc
     3421 ttattctttc tggtcttaag tacttgcctc tcaacctccc tttttagtaa aagacaaggc
     3481 cacgtgagag gcgggactat caacattgtg atgaatttac ttgaaaccca gtgcccaaaa
     3541 tcaatgtagg tagccaagtc caaaaacctg ttctagtcca actagtgaaa tcaaactgtg
     3601 atacttggat aagcttagaa ggaaacgtga agaatacgta gctgctttgg gtttactctg
     3661 gttcagttgg gctgttgaaa tcttaacatc cttgggctta tcacctactg cttgtcagcc
     3721 ctgttccatg tccaggggat gggggtggtg acaatccagt tccaagaccc tcatgctcta
     3781 gagaggaagg tggccagcca gggttgtaac tacgatgaaa aagcagtggg agggtctcct
     3841 atgaggcaag cctaaggaca aaaaggaagg ccttgcagcc tgtattctgg ataaggaatt
     3901 aaaagctcag ttaattgaag ccca
//
