LOCUS       NM_016453               2977 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NCK interacting protein with SH3 domain (NCKIPSD),
            transcript variant 1, mRNA.
ACCESSION   NM_016453
VERSION     NM_016453.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2977)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2977)
  AUTHORS   Kim,H., Oh,H., Oh,Y.S., Bae,J., Hong,N.H., Park,S.J., Ahn,S.,
            Lee,M., Rhee,S., Lee,S.H., Jun,Y., Kim,S.H., Huh,Y.H. and Song,W.K.
  TITLE     SPIN90, an adaptor protein, alters the proximity between Rab5 and
            Gapex5 and facilitates Rab5 activation during EGF endocytosis
  JOURNAL   Exp Mol Med 51 (7), 1-14 (2019)
   PUBMED   31358736
  REMARK    GeneRIF: The findings suggest that SPIN90, as an adaptor protein,
            simultaneously binds inactive Rab5 and Gapex5, thereby altering
            their spatial proximity and facilitating Rab5 activation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2977)
  AUTHORS   Luan,Q., Liu,S.L., Helgeson,L.A. and Nolen,B.J.
  TITLE     Structure of the nucleation-promoting factor SPIN90 bound to the
            actin filament nucleator Arp2/3 complex
  JOURNAL   EMBO J 37 (22) (2018)
   PUBMED   30322896
  REMARK    GeneRIF: Structure of the nucleation-promoting factor SPIN90 bound
            to the actin filament nucleator Arp2/3 complex.
REFERENCE   6  (bases 1 to 2977)
  AUTHORS   Lim,C.S., Park,E.S., Kim,D.J., Song,Y.H., Eom,S.H., Chun,J.S.,
            Kim,J.H., Kim,J.K., Park,D. and Song,W.K.
  TITLE     SPIN90 (SH3 protein interacting with Nck, 90 kDa), an adaptor
            protein that is developmentally regulated during cardiac myocyte
            differentiation
  JOURNAL   J Biol Chem 276 (16), 12871-12878 (2001)
   PUBMED   11278500
REFERENCE   7  (bases 1 to 2977)
  AUTHORS   Hayakawa,A., Matsuda,Y., Daibata,M., Nakamura,H. and Sano,K.
  TITLE     Genomic organization, tissue expression, and cellular localization
            of AF3p21, a fusion partner of MLL in therapy-related leukemia
  JOURNAL   Genes Chromosomes Cancer 30 (4), 364-374 (2001)
   PUBMED   11241789
REFERENCE   8  (bases 1 to 2977)
  AUTHORS   Fukuoka,M., Suetsugu,S., Miki,H., Fukami,K., Endo,T. and
            Takenawa,T.
  TITLE     A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding
            protein, WISH, induces Arp2/3 complex activation independent of
            Cdc42
  JOURNAL   J Cell Biol 152 (3), 471-482 (2001)
   PUBMED   11157975
REFERENCE   9  (bases 1 to 2977)
  AUTHORS   Sano,K., Hayakawa,A., Piao,J.H., Kosaka,Y. and Nakamura,H.
  TITLE     Novel SH3 protein encoded by the AF3p21 gene is fused to the mixed
            lineage leukemia protein in a therapy-related leukemia with t(3;11)
            (p21;q23)
  JOURNAL   Blood 95 (3), 1066-1068 (2000)
   PUBMED   10648423
REFERENCE   10 (bases 1 to 2977)
  AUTHORS   de Bernard,M., Moschioni,M., Napolitani,G., Rappuoli,R. and
            Montecucco,C.
  TITLE     The VacA toxin of Helicobacter pylori identifies a new intermediate
            filament-interacting protein
  JOURNAL   EMBO J 19 (1), 48-56 (2000)
   PUBMED   10619843
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY010884.1, HY050918.1 and
            AF303581.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016453.3.
            
            Summary: The protein encoded by this gene contains a nuclear
            localization signal. It plays a role in signal transduction, and
            may function in the maintenance of sarcomeres and in the assembly
            of myofibrils into sarcomeres. It also plays an important role in
            stress fiber formation This protein is involved in the formation
            and maintenance of dendritic spines, and modulates synaptic
            activity in neurons. The gene is involved in therapy-related
            leukemia by a chromosomal translocation t(3;11)(p21;q23) that
            involves this gene and the myeloid/lymphoid leukemia gene.
            Alternative splicing results in multiple transcript variants of
            this gene. [provided by RefSeq, Aug 2019].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF178432.1, SRR1803615.191540.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000294129.7/ ENSP00000294129.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                HY010884.1         20-31
            13-47               HY050918.1         1-35
            48-2977             AF303581.1         5-2934
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..2977
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /note="NCK interacting protein with SH3 domain"
                     /db_xref="GeneID:51517"
                     /db_xref="HGNC:HGNC:15486"
                     /db_xref="MIM:606671"
     exon            1..279
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..2277
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /note="isoform 1 is encoded by transcript variant 1; SH3
                     protein interacting with Nck, 90 kDa; dia interacting
                     protein; diaphanous protein interacting protein;
                     dia-interacting protein 1; SH3 adapter protein SPIN90; 54
                     kDa VacA-interacting protein; 90 kDa SH3 protein
                     interacting with Nck; 54 kDa vimentin-interacting protein;
                     WASP-interacting SH3-domain protein; wiskott-Aldrich
                     syndrome protein-interacting protein"
                     /codon_start=1
                     /product="NCK-interacting protein with SH3 domain isoform
                     1"
                     /protein_id="NP_057537.1"
                     /db_xref="CCDS:CCDS2776.1"
                     /db_xref="GeneID:51517"
                     /db_xref="HGNC:HGNC:15486"
                     /db_xref="MIM:606671"
                     /translation="MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGE
                     TGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKE
                     TLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGL
                     YQIPLPSSQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSV
                     SSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGTHTTVSQVQPPPSKASAPEPPAEEEV
                     ATGTTSASDDLEALGTLSLGTTEEKAAAEAAVPRTIGAELMELVRRNTGLSHELCRVA
                     IGIIVGHIQASVPASSPVMEQVLLSLVEGKDLSMALPSGQVCHDQQRLEVIFADLARR
                     KDDAQQRSWALYEDEGVIRCYLEELLHILTDADPEVCKKMCKRNEFESVLALVAYYQM
                     EHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALIL
                     AMVFSMGEAVPYAHYEHLGTPFAQFLLNIVEDGLPLDTTEQLPDLCVNLLLALNLHLP
                     AADQNVIMAALSKHANVKIFSEKLLLLLNRGDDPVRIFKHEPQPPHSVLKFLQDVFGS
                     PATAAIFYHTDMMALIDITVRHIADLSPGDKLRMEYLSLMHAIVRTTPYLQHRHRLPD
                     LQAILRRILNEEETSPQCQMDRMIVREMCKEFLVLGEAPS"
     exon            280..389
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            390..594
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            595..706
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            707..1200
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1371
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1458
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1597
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1678
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1807
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1900
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2073
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2977
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2955..2960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /note="hexamer: AATAAA"
     polyA_site      2977
                     /gene="NCKIPSD"
                     /gene_synonym="AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54;
                     WASLBP; WISH"
                     /note="major polyA site"
ORIGIN      
        1 gtagtgtgtc acggggcggg acaaccgcgg cgcggctcgg cgctcccggc ctggcgttgt
       61 ggcgccgcag cccttgccac cttccctgca cctgccctgc ccggcctcat gtaccgcgcg
      121 ctgtacgcgt tccgctcggc ggagcccaac gcgctggcgt tcgccgcggg cgagaccttc
      181 ctggtgctag agcgaagcag cgcgcactgg tggctggccg cgcgggcgcg cagtggtgag
      241 acgggctacg tgccgccagc ctacctgcgc cgcctgcagg gcctggagca ggatgtcctc
      301 caggccattg accgggccat cgaggctgta cacaacacag ccatgcggga tggtggcaag
      361 tacagcctgg aacagcgtgg agtcctccag aagctgatcc accaccggaa agagaccctg
      421 tcacgcagag gcccttcagc ctccagtgtt gcagttatga cctcatcaac cagtgaccac
      481 cacttggatg ctgctgcagc caggcagccc aatggggtgt gtcgagctgg gttcgagcgg
      541 cagcacagcc tacccagttc tgagcatctt ggggcagatg gaggcctcta ccagatccca
      601 cttccatctt cccagatccc accacagcct cgccgagcag cacccaccac accgccccca
      661 ccagtgaagc gccgagaccg cgaggccctg atggcctctg ggagtggtgg ccacaacacc
      721 atgccctccg ggggtaactc tgtgtccagc ggctcctcag tcagcagcac ctccctggac
      781 acgctctata ccagctccag cccatctgaa ccaggctcca gctgctcacc cacaccccca
      841 cctgtgcccc gccgaggcac ccacaccacc gtgtcccaag tccagccccc tccctccaag
      901 gcatcagcac ctgaaccccc tgcagaagaa gaagtggcaa ctggtacaac ctcagcctct
      961 gatgacctgg aagccctggg tacactgagc ctggggacca cagaggagaa ggcagcagct
     1021 gaggcggctg tgcccaggac cattggggcc gagctgatgg agctggtgcg gagaaacact
     1081 ggcctgagcc acgaattatg ccgggtggcc atcggcatca tagtgggtca catccaggcc
     1141 tcggtgccgg ccagctcacc agtcatggag caggtcctcc tctcactcgt agagggcaag
     1201 gacctcagca tggccctgcc ctcagggcag gtctgccacg accagcagag gctggaggtg
     1261 atctttgcag acctggctcg ccggaaggac gacgcccagc agcgcagttg ggcactatat
     1321 gaggatgagg gtgtcatccg ctgctaccta gaggagctgc tgcatattct gactgatgca
     1381 gaccctgaag tttgcaagaa aatgtgcaag agaaacgagt tcgagtctgt cctggccttg
     1441 gtggcctatt accaaatgga acaccgagca tcactgcggc tgctgctcct caagtgcttt
     1501 ggcgccatgt gcagcctgga tgcagccatc atctccacgc ttgtgtcatc cgtgctgcct
     1561 gtagagctgg cgagggacat gcagacagac acgcaggacc accagaaact ctgttactct
     1621 gccctcatcc tggccatggt cttctccatg ggagaggcag tgccctatgc acactatgag
     1681 cacctgggca cgcctttcgc ccagttccta ctgaacatcg tcgaggatgg gctgcccttg
     1741 gacaccacag agcagctgcc ggacctctgc gtgaacctgc ttctggctct caacctgcac
     1801 ctgccagctg ctgaccagaa tgtcatcatg gctgccctga gcaaacacgc caatgtcaag
     1861 atcttctccg agaagctgtt gttgctcctg aacagagggg atgaccctgt gcgcatcttc
     1921 aaacatgagc cacagccacc acactctgtc ctcaagttcc tgcaggacgt gtttggcagc
     1981 ccggccacag ctgccatctt ctaccacaca gacatgatgg ctctcattga catcactgtg
     2041 cggcacatcg cagacctgtc accaggagac aagctgcgca tggagtacct ctccctgatg
     2101 catgctatag tccgcaccac accctacctg cagcaccgcc accggctacc cgacctgcag
     2161 gccatactgc gacgcatcct gaatgaggag gagacctcac cccagtgcca gatggaccgc
     2221 atgattgtcc gagagatgtg caaggaattc ctggtgctgg gggaggctcc cagctagcac
     2281 cttgctgtcc tcccttccct gcagctccag tcagtgtgca ggggactcag gggcttggcc
     2341 ctaagaatgt tttgcactga cagtgggagg ggataaggtg gcagaaggaa cctgagcagg
     2401 acccccacct gaagtagaac ttaagagagg ggccaagtgt aggattgggg accatggtct
     2461 gggagctgtt ctggggcagg gggaatatgc aagctaaagc ccctctatac cctggtaggc
     2521 ttcctcttca gcatgccccc ctcttcaccc cacacccatg tgtacactca gagtcctgct
     2581 gccgctccag gctggaccag acccccatcc ccacccctcc tggtctgtat cctgggcctc
     2641 agcaagccat gcccctgggg tgaggggagg catctcccac cagctctatt ctttgcctta
     2701 gctttgcagt gagtgctgct catgtcctcc cctccacccg cacatggggg tcgctgccct
     2761 tcactcctac ccccagaggc cttggccaag ctgctgcttt gaaagcggga tcttggagac
     2821 aggccatcca tcctggagcc tcatggaaca ggatgatggc actgagaaag ccaatgaccg
     2881 aatctctttt ctctgtaaaa atgtagactg aaaagccatg tgtattttcc tatgtgctgt
     2941 agctctcctt tggaaataaa tcacaggcat ctggaaa
//
