LOCUS       NM_001037806            4882 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens NCK associated protein 5 like (NCKAP5L), transcript
            variant 2, mRNA.
ACCESSION   NM_001037806 NM_020941 XM_374976
VERSION     NM_001037806.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Shintomi,M., Shiratori,M., Negishi,L. and Terada,Y.
  TITLE     Identification of Cep169-interacting proteins and the in vivo
            modification sites of Cep169 via proteomic analysis
  JOURNAL   Biochem Biophys Res Commun 495 (3), 2275-2281 (2018)
   PUBMED   29269292
  REMARK    GeneRIF: Our findings suggest that Cep169 regulates cell growth by
            interacting with multiple proteins.
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Mori,Y., Inoue,Y., Taniyama,Y., Tanaka,S. and Terada,Y.
  TITLE     Phosphorylation of the centrosomal protein, Cep169, by Cdk1
            promotes its dissociation from centrosomes in mitosis
  JOURNAL   Biochem Biophys Res Commun 468 (4), 642-646 (2015)
   PUBMED   26549230
  REMARK    GeneRIF: These data suggest that the dissociation of Cep169 from
            centrosomes is controlled by Cdk1/Cyclin B during mitosis, and that
            Cep169 might regulate MT dynamics of mitotic spindle.
REFERENCE   3  (bases 1 to 4882)
  AUTHORS   Mori,Y., Inoue,Y., Tanaka,S., Doda,S., Yamanaka,S., Fukuchi,H. and
            Terada,Y.
  TITLE     Cep169, a Novel Microtubule Plus-End-Tracking Centrosomal Protein,
            Binds to CDK5RAP2 and Regulates Microtubule Stability
  JOURNAL   PLoS One 10 (10), e0140968 (2015)
   PUBMED   26485573
  REMARK    GeneRIF: These results show that Cep169 targets microtubule tips
            and regulates stability of microtubules with CDK5RAP2.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CN359946.1, CN359941.1, AC131157.4, BC033253.1, BX327000.2 and
            BU617503.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001037806.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by same species and orthologous transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335999.7/ ENSP00000337998.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-645               CN359946.1         13-657
            646-1185            CN359941.1         171-710
            1186-1472           AC131157.4         38051-38337         c
            1473-4384           BC033253.1         1-2912
            4385-4836           BX327000.2         137-588
            4837-4882           BU617503.1         25-70               c
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.12"
     gene            1..4882
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /note="NCK associated protein 5 like"
                     /db_xref="GeneID:57701"
                     /db_xref="HGNC:HGNC:29321"
                     /db_xref="MIM:615104"
     exon            1..92
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            93..154
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            155..313
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..4195
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /note="NCKAP5-like; centrosomal protein of 169 kDa"
                     /codon_start=1
                     /product="nck-associated protein 5-like"
                     /protein_id="NP_001032895.2"
                     /db_xref="CCDS:CCDS41781.2"
                     /db_xref="GeneID:57701"
                     /db_xref="HGNC:HGNC:29321"
                     /db_xref="MIM:615104"
                     /translation="MSEAMDQPAGGPGNPRPGEGDDGSMEPGTCQELLHRLRELEAEN
                     SALAQANENQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNQMLSA
                     LFQQKLQLTTGSLPQIPLTPLQPPSEPPASPSLSSTEGPAAPLPLGHCAGQREVCWEQ
                     QLRPGGPGPPAAPPPALDALSPFLRKKAQILEVLRALEETDPLLLCSPATPWRPPGQG
                     PGSPEPINGELCGPPQPEPSPWAPCLLLGPGNLGGLLHWERLLGGLGGEEDTGRPWGP
                     SRGPPQAQGTSSGPNCAPGSSSSSSSDEAGDPNEAPSPDTLLGALARRQLNLGQLLED
                     TESYLQAFLAGAAGPLNGDHPGPGQSSSPDQAPPQLSKSKGLPKSAWGGGTPEAHRPG
                     FGATSEGQGPLPFLSMFMGAGDAPLGSRPGHPHSSSQVKSKLQIGPPSPGEAQGPLLP
                     SPARGLKFLKLPPTSEKSPSPGGPQLSPQLPRNSRIPCRNSGSDGSPSPLLARRGLGG
                     GELSPEGAQGLPTSPSPCYTTPDSTQLRPPQSALSTTLSPGPVVSPCYENILDLSRST
                     FRGPSPEPPPSPLQVPTYPQLTLEVPQAPEVLRSPGVPPSPCLPESYPYGSPQEKSLD
                     KAGSESPHPGRRTPGNSSKKPSQGSGRRPGDPGSTPLRDRLAALGKLKTGPEGALGSE
                     KNGVPARPGTEKTRGPGKSGESAGDMVPSIHRPLEQLEAKGGIRGAVALGTNSLKQQE
                     PGLMGDPGARVYSSHSMGARVDLEPVSPRSCLTKVELAKSRLAGALCPQVPRTPAKVP
                     TSAPSLGKPNKSPHSSPTKLPSKSPTKVVPRPGAPLVTKESPKPDKGKGPPWADCGST
                     TAQSTPLVPGPTDPSQGPEGLAPHSAIEEKVMKGIEENVLRLQGQERAPGAEVKHRNT
                     SSIASWFGLKKSKLPALNRRTEATKNKEGAGGGSPLRREVKMEARKLEAESLNISKLM
                     AKAEDLRRALEEEKAYLSSRARPRPGGPAPGPNTGLGQVQGQLAGMYQGADTFMQQLL
                     NRVDGKELPSKSWREPKPEYGDFQPVSSDPKSPWPACGPRNGLVGPLQGCGKPPGKPS
                     SEPGRREEMPSEDSLAEPVPTSHFTACGSLTRTLDSGIGTFPPPDHGSSGTPSKNLPK
                     TKPPRLDPPPGVPPARPPPLTKVPRRAHTLEREVPGIEELLVSGRHPSMPAFPALLPA
                     APGHRGHETCPDDPCEDPGPTPPVQLAKNWTFPNTRAAGSSSDPLMCPPRQLEGLPRT
                     PMALPVDRKRSQEPSRPSPTPQGPPFGGSRTPSTSDMAEEGRVASGGPPGLETSESLS
                     DSLYDSLSSCGSQG"
     exon            314..382
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            383..421
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            422..541
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            542..655
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            656..3285
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            3286..3448
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            3449..3530
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            3531..3839
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            3840..3982
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     exon            3983..4882
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4863..4868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /note="hexamer: AATAAA"
     polyA_site      4882
                     /gene="NCKAP5L"
                     /gene_synonym="Cep169; FP1193; KIAA1602"
                     /note="major polyA site"
ORIGIN      
        1 ggcacccccc tttctctgcc ccccgccccc aagccgcggc catggacctg gcggatccgg
       61 cctgccctgg cccagctcaa cacctgattt cggcaaggtg accgttctgg atgctagagt
      121 gagtgtgccc acctgcgtaa gggggcagag acagcctgcc ggggcttctc ttccttgttc
      181 ccagggccag atgtcagagg ccatggacca gccagctggg ggtcctggaa acccaaggcc
      241 aggagagggt gatgatggca gcatggagcc aggcacctgc caggagcttc tgcaccgact
      301 gcgggagctg gaggcagaga actcggcact tgcccaggcc aacgaaaacc agcgggagac
      361 ttatgagcgc tgtctggacg aggttgccaa ccatgtggta caggcgttgc tgaaccagaa
      421 ggacctgcga gaggagtgca tcaagctgaa gaagagagtg tttgacctgg aacggcagaa
      481 ccagatgctg agtgccctgt ttcagcagaa actccagctc acgacaggct cgctccctca
      541 gatcccactc actccactcc agccaccatc agagccacca gcctccccct ccctgagctc
      601 cactgaggga ccggctgccc cgctgcctct ggggcactgt gctgggcaga gagaggtatg
      661 ttgggagcag cagctgaggc caggaggccc aggcccccca gccgccccac ccccagcgct
      721 ggatgcccta tccccgttcc ttcggaagaa ggcccagatt ctggaggtgc tgagagccct
      781 ggaagagact gaccccttgc ttctctgctc acctgccacc ccctggcggc ctccaggcca
      841 ggggcctggc tccccagagc ccatcaacgg cgagctgtgt ggcccgcctc agcctgaacc
      901 ctcaccctgg gcgccctgcc tgctgctagg ccctggcaac ctgggaggcc tgctgcactg
      961 ggagcgcctc ttggggggtc tgggagggga agaggacact gggcggccct ggggtcctag
     1021 caggggacct cctcaggccc agggcaccag ctctggccca aactgtgccc caggcagcag
     1081 ctcctcctcc tcttctgatg aggcaggtga ccccaatgag gcacccagcc ccgacaccct
     1141 gctcggtgcc ctggcccgca gacagttgaa cctgggccag ctccttgagg acacagagtc
     1201 ttacctacag gccttcctgg ccggggctgc aggcccactc aatggggacc acccaggtcc
     1261 tgggcagtca tcctccccag accaggcgcc cccacagctg tctaagtcca aaggcctccc
     1321 caagtcagct tggggtgggg gtaccccaga ggcccacagg ccaggcttcg gtgctacctc
     1381 agagggccag gggcccctcc ccttccttag catgttcatg ggtgctgggg atgccccact
     1441 gggctctcgg cctggccacc cccattcctc atctcaggtg aaaagcaagc tccaaattgg
     1501 ccccccttct cctggggaag ctcagggacc ccttctgccc tctccagcta ggggtctcaa
     1561 gtttctaaag ctgcctccaa cctcggagaa gagccccagc ccaggaggcc cgcagctcag
     1621 tccccagctc ccccggaact cgcgaatccc ctgtcggaac agtggctcag acggcagccc
     1681 ctccccactg ttggcccgaa ggggtctggg tggaggagag ctgtccccag agggggcgca
     1741 aggcctgccc accagccctt caccctgcta cacaacccca gactccacac agctcagacc
     1801 cccgcagtca gccttgtcca ccacgctgtc cccaggccca gtggtgtctc cctgctatga
     1861 gaacattctg gacctttctc ggagcacctt tagggggcct tccccagagc cacctccatc
     1921 cccactgcag gtgcccacct acccacagct aactctggag gtaccacagg cccctgaggt
     1981 cctcagaagc cctggagtac cccccagtcc ttgcctccca gaatcgtacc cctatgggag
     2041 cccccaagag aagagtttgg acaaggcagg ctcggagtct ccccatcccg gccgcaggac
     2101 cccaggcaac tcatccaaga agcccagcca ggggtcagga cggcgacctg gggatcctgg
     2161 cagcacacct ctgcgggaca gactggcggc cctggggaag ctgaagacag gccccgaggg
     2221 ggccctgggc tcagagaaga atggggtgcc agccaggcct ggcaccgaaa agacccgggg
     2281 acctgggaag tcaggggaga gtgctggaga catggtgccc tccatccaca ggccactgga
     2341 gcagctagaa gccaaggggg ggatacgggg ggcagtggcc ttgggcacaa acagcctgaa
     2401 gcagcaggaa cctggactta tgggggatcc cggggcccga gtctactcct ctcactccat
     2461 gggggcccgg gtggacctgg agcctgtctc accaaggagc tgcctcacca aagtggagct
     2521 ggccaagagc cggctggcag gggccctgtg cccccaggta ccccgtaccc ctgccaaagt
     2581 gccaacctca gccccaagcc tgggcaagcc caataagagc cctcacagca gccccaccaa
     2641 gctcccttcc aagtcaccaa ccaaggtggt gcctcgacct ggggctccgc tagtcaccaa
     2701 ggagtccccc aagcctgaca aagggaaggg ccctccctgg gcagactgtg gtagtaccac
     2761 ggcccagtcc acacccctag tacctggccc cactgaccca agtcagggcc ctgaggggct
     2821 ggccccacac tcagccatcg aggagaaggt gatgaagggc attgaggaga acgtgctgcg
     2881 gctccagggc caggagcgag cccctggcgc cgaggtcaag caccgcaaca ccagcagcat
     2941 cgccagctgg ttcggcctta agaagagcaa gctgccagcg ctgaaccgcc gcacagaggc
     3001 caccaagaac aaggaggggg ctggcggggg ctccccgctc cggagggaag tcaagatgga
     3061 agcccggaag ctggaggccg agagcctcaa catctccaag ctgatggcca aggcggaaga
     3121 cctgcgtcgg gcactggaag aggagaaggc ctacctaagc agccgggccc ggccacggcc
     3181 tggtggccca gccccagggc ccaacacggg gctggggcag gtgcagggcc agctggctgg
     3241 catgtaccaa ggtgcagaca ccttcatgca gcagctgcta aacagggtgg atggcaagga
     3301 gctgccatcc aagagctggc gggagcccaa gcctgagtac ggggatttcc agccggtgtc
     3361 ttctgacccc aagagcccct ggccagcctg tgggccccgg aatggcctgg tgggccctct
     3421 tcagggctgc ggaaaacctc ctggaaagcc gagcagcgag ccagggaggc gggaagagat
     3481 gccctcggag gacagcctgg ccgagccagt gcccacctca cacttcacag cctgtggctc
     3541 cttgactcga accttggaca gtggcattgg gaccttccca cccccagacc atggtagcag
     3601 tgggaccccc agcaagaatc ttcctaagac caagccaccg cggctggatc ccccacctgg
     3661 ggtaccccca gctcggcccc caccccttac caaagtcccc cgccgcgccc acacactgga
     3721 gcgggaggtg ccaggcatag aggagctgct ggtgagtggg cggcacccca gcatgccagc
     3781 cttccctgca ctgctacccg ctgctccggg ccaccggggc catgagacct gtcctgatga
     3841 tccctgtgaa gacccaggcc ccacccctcc tgtccagctg gccaagaact ggaccttccc
     3901 caatactagg gcagccggca gctcctcgga ccctctcatg tgcccacccc gacaactgga
     3961 ggggctgccc aggaccccca tggccctgcc cgtggaccgg aagcgaagcc aggagcccag
     4021 ccgcccgtcc cctacgcccc agggcccacc tttcgggggt agccgcaccc ccagcacttc
     4081 ggacatggcc gaggaaggca gagtggccag cgggggcccc ccagggctgg agacctcgga
     4141 gtctctcagt gactcactct acgactcgct gtcctcttgt gggagtcagg gctgaggggc
     4201 tgcgccacgc cacggccccg ctggagctgg ggaccacaga ctggaccggc tctcttcatg
     4261 cccagccccc ggagacgggg acccctccct gaagggacca aggaggcagg tggataagaa
     4321 ggtgaaaagg gggtccctgg cacaccccac cacccactgc ttcggcggat gagatgaccg
     4381 tgctcagctc agggagagac cccgcccttg gtcccttctc tcacccagag taaggctctt
     4441 cctggaaggg actgggggtt aaaggccact gtgtcgcagc cctccagtcc ctacttcagg
     4501 ctgagccatc ttgtggtgct gggcttcctg cccaccagcc gtgccatctc tgcccaaccc
     4561 ggctgctcct ctgccccgaa gccctcgcga ggccctcctg gaggcccccg tgctggtgga
     4621 gtttgggggc cagggggaca agttgccttc tctctctgcc ctggtcctcc ctgctgtctg
     4681 gatggtgctg ccctcctctg ccccatgcct ttggggtctg ttcgtccgtc ttttttgttg
     4741 ttgtttttat atattgaagc gcctggccca gcccccagcc cccagccccg cactgcggtt
     4801 aatttatgtg ttgtttaaaa tgcggctgct ctgcttcctg cctctgcttc tgccgtatcc
     4861 ctaataaaat gtggaggccc cc
//
