LOCUS       NM_005337               8980 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript
            variant 1, mRNA.
ACCESSION   NM_005337
VERSION     NM_005337.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Wang,X., Dou,J., Liu,M., Zhang,Y., Li,Y. and Tong,Z.
  TITLE     Potential predictive value of immune-related genes FUCA1 and
            NCKAP1L for osteosarcoma metastasis
  JOURNAL   Gene 927, 148645 (2024)
   PUBMED   38844271
  REMARK    GeneRIF: Potential predictive value of immune-related genes FUCA1
            and NCKAP1L for osteosarcoma metastasis.
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Cook,S., Lenardo,M.J. and Freeman,A.F.
  TITLE     HEM1 Actin Immunodysregulatory Disorder: Genotypes, Phenotypes, and
            Future Directions
  JOURNAL   J Clin Immunol 42 (8), 1583-1592 (2022)
   PUBMED   35869404
  REMARK    GeneRIF: HEM1 Actin Immunodysregulatory Disorder: Genotypes,
            Phenotypes, and Future Directions.
            Review article
REFERENCE   3  (bases 1 to 8980)
  AUTHORS   Huang,Y., Ren,L., Li,J. and Zou,H.
  TITLE     Long non-coding RNA PVT1/microRNA miR-3127-5p/NCK-associated
            protein 1-like axis participates in the pathogenesis of abdominal
            aortic aneurysm by regulating vascular smooth muscle cells
  JOURNAL   Bioengineered 12 (2), 12583-12596 (2021)
   PUBMED   34898354
  REMARK    GeneRIF: Long non-coding RNA PVT1/microRNA
            miR-3127-5p/NCK-associated protein 1-like axis participates in the
            pathogenesis of abdominal aortic aneurysm by regulating vascular
            smooth muscle cells.
REFERENCE   4  (bases 1 to 8980)
  AUTHORS   Castro,C.N., Rosenzwajg,M., Carapito,R., Shahrooei,M., Konantz,M.,
            Khan,A., Miao,Z., Gross,M., Tranchant,T., Radosavljevic,M.,
            Paul,N., Stemmelen,T., Pitoiset,F., Hirschler,A., Nespola,B.,
            Molitor,A., Rolli,V., Pichot,A., Faletti,L.E., Rinaldi,B.,
            Friant,S., Mednikov,M., Karauzum,H., Aman,M.J., Carapito,C.,
            Lengerke,C., Ziaee,V., Eyaid,W., Ehl,S., Alroqi,F., Parvaneh,N. and
            Bahram,S.
  TITLE     NCKAP1L defects lead to a novel syndrome combining
            immunodeficiency, lymphoproliferation, and hyperinflammation
  JOURNAL   J Exp Med 217 (12) (2020)
   PUBMED   32766723
  REMARK    GeneRIF: NCKAP1L defects lead to a novel syndrome combining
            immunodeficiency, lymphoproliferation, and hyperinflammation.
REFERENCE   5  (bases 1 to 8980)
  AUTHORS   Hu,M., Xie,J., Hou,H., Liu,M. and Wang,J.
  TITLE     Prognostic Value of DNA Methylation-Driven Genes in Clear Cell
            Renal Cell Carcinoma: A Study Based on Methylation and
            Transcriptome Analyses
  JOURNAL   Dis Markers 2020, 8817652 (2020)
   PUBMED   32733620
  REMARK    GeneRIF: Prognostic Value of DNA Methylation-Driven Genes in Clear
            Cell Renal Cell Carcinoma: A Study Based on Methylation and
            Transcriptome Analyses.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8980)
  AUTHORS   Joshi,A.D., Hegde,G.V., Dickinson,J.D., Mittal,A.K., Lynch,J.C.,
            Eudy,J.D., Armitage,J.O., Bierman,P.J., Bociek,R.G., Devetten,M.P.,
            Vose,J.M. and Joshi,S.S.
  TITLE     ATM, CTLA4, MNDA, and HEM1 in high versus low CD38 expressing
            B-cell chronic lymphocytic leukemia
  JOURNAL   Clin Cancer Res 13 (18 Pt 1), 5295-5304 (2007)
   PUBMED   17875758
REFERENCE   7  (bases 1 to 8980)
  AUTHORS   Weiner,O.D., Marganski,W.A., Wu,L.F., Altschuler,S.J. and
            Kirschner,M.W.
  TITLE     An actin-based wave generator organizes cell motility
  JOURNAL   PLoS Biol 5 (9), e221 (2007)
   PUBMED   17696648
  REMARK    GeneRIF: the Hem-1/Nap1 component of the Scar/WAVE complex
            localizes to propagating waves that appear to organize the leading
            edge of a motile neutrophil
REFERENCE   8  (bases 1 to 8980)
  AUTHORS   Weiner,O.D., Rentel,M.C., Ott,A., Brown,G.E., Jedrychowski,M.,
            Yaffe,M.B., Gygi,S.P., Cantley,L.C., Bourne,H.R. and Kirschner,M.W.
  TITLE     Hem-1 complexes are essential for Rac activation, actin
            polymerization, and myosin regulation during neutrophil chemotaxis
  JOURNAL   PLoS Biol 4 (2), e38 (2006)
   PUBMED   16417406
  REMARK    GeneRIF: versatile Hem-1-containing complexes coordinate diverse
            regulatory signals at the leading edge of polarized neutrophils,
            including but not confined to those involving Verprolin-homologous
            protein-dependent actin polymerization
REFERENCE   9  (bases 1 to 8980)
  AUTHORS   Baumgartner,S., Martin,D., Chiquet-Ehrismann,R., Sutton,J.,
            Desai,A., Huang,I., Kato,K. and Hromas,R.
  TITLE     The HEM proteins: a novel family of tissue-specific transmembrane
            proteins expressed from invertebrates through mammals with an
            essential function in oogenesis
  JOURNAL   J Mol Biol 251 (1), 41-49 (1995)
   PUBMED   7643388
REFERENCE   10 (bases 1 to 8980)
  AUTHORS   Hromas,R., Collins,S., Raskind,W., Deaven,L. and Kaushansky,K.
  TITLE     Hem-1, a potential membrane protein, with expression restricted to
            blood cells
  JOURNAL   Biochim Biophys Acta 1090 (2), 241-244 (1991)
   PUBMED   1932118
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA820115.1, BC093769.2,
            BC093771.1, M58285.1, AW575485.1 and AC068789.16.
            
            On Nov 23, 2018 this sequence version replaced NM_005337.4.
            
            Summary: This gene encodes a member of the HEM family of
            tissue-specific transmembrane proteins which are highly conserved
            from invertebrates through mammals. This gene is only expressed in
            hematopoietic cells. The encoded protein is a part of the Scar/WAVE
            complex which plays an important role in regulating cell shape in
            both metazoans and plants. Alternatively spliced transcript
            variants encoding different isoforms have been found.[provided by
            RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.269017.1,
                                           SRR1803617.36686.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000293373.11/ ENSP00000293373.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DA820115.1         42-47
            7-2544              BC093769.2         1-2538
            2545-3510           BC093771.1         2539-3504
            3511-3833           M58285.1           3513-3835
            3834-3852           AW575485.1         17-35               c
            3853-8980           AC068789.16        15063-20190
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13-q13.2"
     gene            1..8980
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="NCK associated protein 1 like"
                     /db_xref="GeneID:3071"
                     /db_xref="HGNC:HGNC:4862"
                     /db_xref="MIM:141180"
     exon            1..140
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..3422
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="isoform 1 is encoded by transcript variant 1;
                     membrane-associated protein hem-1; hematopoietic protein
                     1; nck-associated protein 1-like"
                     /codon_start=1
                     /product="nck-associated protein 1-like isoform 1"
                     /protein_id="NP_005328.2"
                     /db_xref="CCDS:CCDS31813.1"
                     /db_xref="GeneID:3071"
                     /db_xref="HGNC:HGNC:4862"
                     /db_xref="MIM:141180"
                     /translation="MSLTSAYQHKLAEKLTILNDRGQGVLIRMYNIKKTCSDPKSKPP
                     FLLEKSMEPSLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFR
                     DHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNC
                     AHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQG
                     AEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQ
                     CQKLWKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSG
                     QFHCQRRQFLRMAVKELETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTK
                     TKTPEDYADSSIAELLFLLEGIRSLVRRHIKVIQQYHLQYLARFDALVLSDIIQNLSV
                     CPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWFRLQAYTSVAKAPLHLHEN
                     PDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESAMLRYAI
                     AFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLS
                     EQLLPKHCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLN
                     LTELALTMNHVYSFSVFEHTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSEL
                     LAGVKAYIGFIQSLAQFLGADASRVIRNALLQQTQPLDSCGEQTITTLYTNWYLESLL
                     RQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRALAELLGPYGMKFLSENL
                     MWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTIIGVI
                     LSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDI
                     DPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNI
                     HCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLL
                     MRLVVEESSFLTLDMLESCFPYVLLRNAYREVSRAFHLN"
     exon            141..251
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            252..344
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            345..401
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            402..544
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            545..635
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            636..773
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            774..822
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            823..979
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1036
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1133
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1243
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1376
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1458
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1517
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1663
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1796
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1916
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2062
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2194
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2413
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2544
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2642
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2736
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..2819
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2900
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2901..2994
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3111
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            3112..3221
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            3222..3311
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     exon            3312..8980
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3679..3684
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="hexamer: AATGAA"
     polyA_site      3705
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="major polyA site"
     regulatory      3817..3822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="hexamer: ATTAAA"
     polyA_site      3852
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="major polyA site"
     regulatory      8172..8177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="hexamer: ATTAAA"
     regulatory      8176..8181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="hexamer: AATGAA"
     polyA_site      8196
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
     polyA_site      8221
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
     polyA_site      8224
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
     regulatory      8955..8960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
                     /note="hexamer: ATTAAA"
     polyA_site      8980
                     /gene="NCKAP1L"
                     /gene_synonym="HEM1; IMD72"
ORIGIN      
        1 agacattgct gtctggtgct cctctctcag tggccatcat gtctttgaca tctgcttacc
       61 agcataaatt agcagagaag ctcactatcc tgaatgatcg cggtcagggg gttctcatcc
      121 gtatgtataa catcaagaag acttgttcag accccaaatc taagccacct ttcttactgg
      181 aaaagtccat ggaaccatct ctcaagtata tcaacaagaa atttcccaac atagatgtcc
      241 gaaacagcac gcaacattta ggaccagtac atcgtgaaaa agccgagata attagattcc
      301 tcaccaacta ctaccagtca tttgtggatg tcatggaatt tcgggatcat gtatatgaac
      361 ttctcaacac cattgatgcc tgccagtgcc attttgatat caatctcaac tttgatttca
      421 ctcggagtta cctggacttg attgtaactt acacctcagt cattttactt ctgtcacgga
      481 ttgaagatcg gcggatactc attggcatgt acaattgtgc ccatgagatg ctgcatgggc
      541 atggtgaccc cagttttgcc cgtctgggtc agatggtctt ggagtatgac caccctctga
      601 agaagctgac agaagagttt gggcctcaca caaaggctgt gagtggagcc ctcctctctt
      661 tgcatttcct ctttgtccga agaaaccagg gggctgagca gtggcgcagt gcccaacttc
      721 taagcctcat cagcaacccc ccagccatga ttaaccctgc taattcagat acaatggcct
      781 gtgagtatct gtctgtggaa gtaatggagc gctggattat cattgggttt cttctttgtc
      841 atgggtgcct caactccaat agccagtgcc agaagctgtg gaagctgtgt ctgcagggct
      901 ccctctacat cacccttatc cgtgaggatg tgctgcaggt gcacaaagtc accgaggacc
      961 tgtttagcag tttgaaaggg tatggcaaga gagtggcaga cataaaggag agcaaggaac
     1021 atgtaattgc aaacagtggc cagtttcatt gtcaacggcg gcaatttctg cggatggcag
     1081 tgaaggagct ggagactgtg ttggctgatg aaccgggact actgggtcct aaggctcttt
     1141 ttgctttcat ggccctgtcc ttcattcgtg atgaggtcac ctggctggtt cgccacacag
     1201 agaatgtcac caagacaaag acacctgagg actatgctga ctcgagcatt gcagagctac
     1261 ttttcttgtt ggaggggatt aggtctctgg tccgaagaca catcaaagtg atacagcaat
     1321 accaccttca gtacttggca agatttgatg ctcttgtgct cagtgacatc attcagaact
     1381 tgtctgtgtg tccagaggag gagtccatca tcatgtcctc attcgtcagt atcctctcct
     1441 ctctgaatct caaacaagtt gataatggag aaaaatttga attctcagga ttgaggctgg
     1501 actggttccg cctacaggca tacactagcg tggctaaggc ccctctgcac ctgcatgaga
     1561 accctgactt agccaaggtg atgaacctca ttgtcttcca ctcccgaatg ctggactccg
     1621 tagaaaaatt gctggtggaa acttctgatc tgtctacttt ctgctttcat cttcgtatct
     1681 ttgagaagat gtttgccatg accttggagg aatctgccat gttgcgttat gccattgctt
     1741 tccccctgat ttgtgctcac tttgtccact gcactcatga gatgtgccca gaggagtacc
     1801 cccacctcaa gaaccatggt cttcaccact gcaactcctt cctggaagag ttggccaagc
     1861 agaccagcaa ttgcgtcctg gagatctgtg ctgagcagcg aaacctgagc gagcagcttc
     1921 tacctaagca ctgtgccact acaatcagca aagccaagaa caagaaaacc aggaagcaga
     1981 ggcagactcc cagaaaagga gagcccgaga gggacaagcc aggagctgag agtcaccgga
     2041 agaaccgcag cattgtcacc aacatggaca agctacacct aaacttgaca gaactggcac
     2101 tgacaatgaa tcatgtatac agtttctccg tgtttgaaca tactatcttc ccttctgagt
     2161 acctcagcag ccacctggag gccagactca acagagccat tgtgtggctg gctggctaca
     2221 atgccacgac ccaggagatc gtacggcctt ctgagctgtt ggcaggagtc aaagcataca
     2281 ttggtttcat acagtcactg gcccagtttt tgggtgcaga tgcttccaga gtcatccgca
     2341 acgccctcct gcagcagaca caaccactgg attcctgtgg ggaacagaca atcaccacac
     2401 tctacacaaa ctggtacctg gaaagtctgc ttagacaggc aagcagtggg accatcatcc
     2461 tctccccagc catgcaggcc ttcgtcagcc tgcccagaga aggggagcag aacttcagtg
     2521 cagaggagtt ctctgacatc tctgagatgc gggccttggc agaactcctg ggcccctatg
     2581 gcatgaagtt cctgagtgaa aacctgatgt ggcatgtgac ctctcagatt gtggagctga
     2641 agaagctggt ggtggaaaac atggacatac ttgttcagat cagatccaac tttagcaagc
     2701 cggacttgat ggcttccctg ctgccccagc tgacaggggc tgaaaatgtg ctaaagcgca
     2761 tgaccatcat tggggttatc ctcagtttca gggccatggc ccaagaggga cttcgggagg
     2821 ttttctcctc ccactgccca tttcttatgg gtcccattga gtgcttgaag gagtttgtca
     2881 ctccagacac agacatcaag gtgaccttga gtatctttga gctggcatct gctgcaggtg
     2941 tgggctgtga cattgaccca gccttggtgg ctgccattgc taatctgaaa gctgatactt
     3001 catctcctga ggaggaatat aaggtggcct gcctgctctt gatctttctg gcagtttccc
     3061 tcccactcct tgccactgac ccttcttcct tttatagcat tgagaaggat ggttacaaca
     3121 acaatattca ttgcttgacc aaagccatca tccaggtgtc tgctgccctc ttcacgctct
     3181 acaacaagaa cattgaaact cacctcaagg aatttctggt ggtggcctct gtcagcctct
     3241 tgcagctggg ccaggagact gacaagctta aaaccagaaa tcgagaatcc atttctctgc
     3301 tcatgcgctt ggtggtggag gagtcatcct tcctgaccct ggacatgctg gagtcctgtt
     3361 tcccttatgt cctgcttcga aatgcctatc gggaggtgtc tcgggccttc cacctaaact
     3421 gaatgcctgc cagtacccac tgaagagccc tttggacctt cctaaaccct tgccatagtg
     3481 gaagctgtgg tcactttcgc agggggtggg aatggggtgg ggtcactaag gagagagggt
     3541 caggagccag agttgatgag cagatctgtg gaagaacaat ccagggctga gaaatcgtag
     3601 agcagtgagg caggctggga gcatggagga cagcttatgg aaaaagttag ggcgtggggc
     3661 cacatgtgtg aattttacaa tgaaaaaagg agtaacgtac aagtatattt tctatcttct
     3721 ggtgacttga gcttgagctc tgacaggcat gggcctctcc gaccttcatc actattctta
     3781 ggataatgct ggcgggcaga gatgatcaat catcatatta aatcataatg agcttataat
     3841 cctcccactg gagctgctat tgtctcctgc acattcatta ggaccattat cttctctctt
     3901 cctttttcaa atgtgttcag caaacattca acctgctcct actgcagagt agcagcaaag
     3961 gagcccctct tttcctttgt ggggctcttc gaggggtcat tgggtctctt cctttgcccc
     4021 cagcttgact gcgtttccta ctggggcaag gaggaagtgc ccttcagaga tgttcactgc
     4081 ccacctactc tactacattg tttgttacaa gttctctctt gctttaaccc ctcctgcttt
     4141 gatatctcca caacccagag cagagggtca gttagtctcc gtcaattcta ggttacatga
     4201 caactgattg gcccagactt aggtgggttt tccctcctgt accatttggg ggtactctgc
     4261 cttttattgg gagggactaa tttaacccca aatccttttg tcagcctggg tatagctgtt
     4321 gctccaggaa gggatttctc ttctggatct taaatttcac agacctcatt aggttgtatg
     4381 gccctgagag tgggtatcct tgagggagta caagctgttt caacttagcc cttttctgcg
     4441 ctaattagaa tttcaagcgt cacagagcct gggggctttc aagatagcac taaactaggt
     4501 ttacttaact ccttggcagg aaatagtggc ccccatgcat cacttctgtg aatccactgc
     4561 cagggagaag tggagaaaca gatcatatat gtcacaggtc tagagtggtt ggaattgaca
     4621 aatataatta gctagttggt taattagggt catgtccata ctttcatgat gaaagggaag
     4681 cagttgtcgg ttttcagcaa aaacgattca gccttcctga agcctatttg atagctgttt
     4741 tggggttttg tacatgtgtt tgctttttat ttcttgagac ccaggcttag tcaatacttt
     4801 cttaacttgg cacccataat caacatacag atttttctta gcaacagttg tctgctcttt
     4861 agggtggaag cgatagagaa tcctgcttcg tgcatcagca aatcctcctt ccttgctatg
     4921 ggaagggctt atgttctaaa gggtgattca gtgtcaggct tatgagactg agagtaaagt
     4981 tgatgaggaa gaggaggctc agaagtatct gaacaactac tctatatgtt tatatttttc
     5041 tgcttgaggg gatagagatc cctcctccat ctgcatctgc atatagaact gcttaagagg
     5101 acagcaagac aagttgctgc agggggatgg agggtagaca gaatgccttt ttttaaaact
     5161 gaaaatttta ttgagatact taatccacct gcacttgtaa gaaatcatac agagaaattc
     5221 tattttcctt tacacagttt ccctgaatgc taacatctag caaaactgta gtacactgcc
     5281 acaaccagga taactgacat tgatataatc tactgatctt gttcagattt ccttccccag
     5341 ttttgcttat gttcatttct ctctgtgtgt gcacgtgtgt gtgttatcta tttctataca
     5401 gctttatcat gtgtaggttt gtgtatctgc cactgcagtc aacatacaga acaggccggg
     5461 cgcagtggct cacgcctgta atcccagcac tttgggaggc caaggcaggt ggatcacctg
     5521 aggtcagggg tttgagagca gcctggccaa tatagtgaaa caccgtctct actaaaaata
     5581 ccaaaactag ccgggcgtgg tggtgcatgc ctgtagtccc actactcagg aggctgaggc
     5641 agcagaataa cttgatcccg ggaggcggag gttgcagtaa gctaagatcc tgccacagca
     5701 ttccaggctg agtgacagag cgagactctg tctcaaaaaa taaataataa tttaaaaaaa
     5761 gatacagaac agttccatca ccacaaggat ccctcgcatt gctcttttat agcaatttta
     5821 tagcaatttt ctcctcccac aacccccttc cccatctcta gctcctggta acacagatat
     5881 attctctatt tctaaaattt tgtcatttga tatatctttt tgtaaacata taattataca
     5941 cacatatttc tctaccactt atactttttc aaagtgcttt cacatcctta tagcaattct
     6001 gcaaggtagc cagggaaggc tttcctattt caggtcagaa aacagattta gaaatattaa
     6061 atgacttaat gaaggtcaca tgtttagcag tagaactaga actgggaccc aagtctgatg
     6121 ttcagtccta gaccttttct acttgcccgg tctgcctctt cacaagggaa gacttgggtt
     6181 ttagaatcca gagaccttag aaggagcaaa ttggagaagg tgaggacatg atggccttta
     6241 ctaccggaag caaaaagtga atgtccagag gtgaagggac agagaagtaa caggacccat
     6301 cagcatttca ggcaatttgc ttcctttacc aggagggtct ctgtcctctt acaacctggg
     6361 aaaaaataag ttgtgtccca ggttgtaagt ttgctttact taggcccaaa ggaactaaag
     6421 aatggatatc agaaatctca ccccaattgt tttgtcttac aggcacagaa taggttctag
     6481 cctcagcatt ttcattaatg tagtgttttt attaacatct ccattaactt gcttgcagca
     6541 atgagatgga attatgcctc ccacaaggcc tggggcaaga tacttgcata ggtgccccct
     6601 tttgtttgga cagtagaaac actgcctccc ctgccatggt gttcaatcca gttggcaggt
     6661 gggccagctt caaagtgcag aaggaactgg gtgtggtggc tcacccctgt aatcccagca
     6721 ctttgggagg caggcggatc gcttgaactc aggagtttga gaccactctg ggcaacacag
     6781 tgaaaccctg tctctccaaa aatacaaaaa attagctggg catggtggct gttgtctgta
     6841 atcccaacac tttgggaggc tgaggccagt ggatctcttg agctaaggag gttaagacca
     6901 gcctggtcaa catggcgaac tccgtctcta caaaaaaata cgaaaattag ctgggcgtag
     6961 tagttcctgc ctgtagtcct agctacttgg aaggctgagg tggggaggat cgcttgagtc
     7021 cgggaggcag aggttgcagt gagcgaagat gatgccattg cactccagtc tgggtgacag
     7081 agcaagatcc tgtctcacaa aaaaaaaaaa aaaaaaaaat gcagaaggtc cagggcctgg
     7141 ccaaggggat gatgccctgt agcactttgc tttgcaggaa gggaacaact gtgctgtgaa
     7201 taccaggatc caggagggag ggagggtata tctgtgtccc ccagctaggg acaaggcttc
     7261 cttaacctta ggtcctcatg cttatattag ttcttctcca acaaagtttg actttgagaa
     7321 gaagctggca catggctgaa atgcactcac gcggttgggg gaagagtgtt cttccagtcc
     7381 tcctccccac agggcctcct gcttctccta agtacctgtc atcaaaggag atgaagatga
     7441 atcccccttc agcccccaca cctcaatgag ttgtcaagta ctctccctcc cacttgaata
     7501 ggaagcagga gcagcaggcc aggttgcagg ttgaggaagt cagaagttaa cttgagctga
     7561 gtttgcagct ctggagagat tgaggagcag acttttctct tgtcactttg gaaggagagg
     7621 attgtgaggc tgccccttta aatagcattc ccgtaggagg cagatcacat ccatccgtca
     7681 cctactgcca aagcatcatt tttggtttct atcaaactca aagcttgttg gaaaggattg
     7741 tggggttagg ggagtgagga ggcagagcct cctaggggta gggggcagtt tagaagctga
     7801 aaaggagagg aatgagaagg agcctgggaa aggtggggga tgaggacaag aggaaaagac
     7861 agggagagaa tagttgtctt ttgagcctca gagtggaaca agtataccca attcttatct
     7921 gtttagagac gcagcaaggt agaaatggga ttgtattgca acaagaggaa tgaggttgag
     7981 actaaacacc aggactggtg actaaagaaa gtgaaaggag ccacagagtg aaaaaggctg
     8041 atagacttgg ctctggtttt atcacttact ctctgtgaga ccctataatt tttctgggtt
     8101 agtttagttc tcatttattt ttatttttac tttttccatc aaactgtgtt gtctatgaag
     8161 ttttcccatt tattaaatga agggaattag agtggatgaa ctctaatgtc tctttcacga
     8221 ataactttgt gtgtgtgtgc gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gaattagcct
     8281 taaaatgaat caggagcagg gggtcttggg agagcaggat gtgacactgt gttaacaaga
     8341 aatgttgaga gagccctctg gatgtatgtc ttgcctcttt cccacctatt ctagtttaaa
     8401 gacctgccta aatttggaaa ctccttagct ctaatcctaa tttactaatt agagctcttt
     8461 agcaccatcc taatttgcaa ttcttttgta gtttccagcc catttcattt tcagttgata
     8521 aggaggagat tgctcccaaa ttttcagaaa tggactgtgt aggagtgtga ctagggtctc
     8581 agatgcagcc atttcttggc tgggtctggg gaaaagccaa gggatgtgtg ctaaaggtag
     8641 atggacagag ggtgggaagt cccttattat ttccactttt ttctccatct ctgactctgc
     8701 catctctttc tgcctctgta ctttctggga gctggttctg ctgatacttc tgctgaatct
     8761 aaatgctgac ctaacttggc aattctttta tgttaggagt cttagctgtg acagttcatc
     8821 cgcttcttcg cccttccatt tcacttcttt tctccttctc tgactgggag aggaggagcc
     8881 tccactcagt atttagaaca gagtaaatac cttctcctga tcactcctcc ttccaacctc
     8941 cagaattttt tgaaattaaa attcacattc tgggatcctg
//
