LOCUS       NM_003581               2666 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NCK adaptor protein 2 (NCK2), transcript variant 1,
            mRNA.
ACCESSION   NM_003581
VERSION     NM_003581.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Song,X., Xiao,B., Lu,J., Zhang,W., Li,J., Zhu,X., Sun,Z. and Li,L.
  TITLE     [CBL inhibits proliferation and invasion of breast cancer cells by
            ubiquitylation-mediated degradation of NCK2]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 42 (11), 1594-1603 (2022)
   PUBMED   36504051
  REMARK    GeneRIF: [CBL inhibits proliferation and invasion of breast cancer
            cells by ubiquitylation-mediated degradation of NCK2].
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2666)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2666)
  AUTHORS   Hartl,F.A., Ngoenkam,J., Beck-Garcia,E., Cerqueira,L., Wipa,P.,
            Paensuwan,P., Suriyaphol,P., Mishra,P., Schraven,B., Gunther,S.,
            Pongcharoen,S., Schamel,W.W.A. and Minguet,S.
  TITLE     Cooperative Interaction of Nck and Lck Orchestrates Optimal TCR
            Signaling
  JOURNAL   Cells 10 (4), 834 (2021)
   PUBMED   33917227
  REMARK    GeneRIF: Cooperative Interaction of Nck and Lck Orchestrates
            Optimal TCR Signaling.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2666)
  AUTHORS   Park,S., Takeuchi,K. and Wagner,G.
  TITLE     Solution structure of the first SRC homology 3 domain of human Nck2
  JOURNAL   J Biomol NMR 34 (3), 203-208 (2006)
   PUBMED   16604428
  REMARK    GeneRIF: Molecular model of the first SRC homology 3 domain of
            Nck2.
REFERENCE   6  (bases 1 to 2666)
  AUTHORS   Tu,Y., Li,F., Goicoechea,S. and Wu,C.
  TITLE     The LIM-only protein PINCH directly interacts with integrin-linked
            kinase and is recruited to integrin-rich sites in spreading cells
  JOURNAL   Mol Cell Biol 19 (3), 2425-2434 (1999)
   PUBMED   10022929
REFERENCE   7  (bases 1 to 2666)
  AUTHORS   Braverman,L.E. and Quilliam,L.A.
  TITLE     Identification of Grb4/Nckbeta, a src homology 2 and 3
            domain-containing adapter protein having similar binding and
            biological properties to Nck
  JOURNAL   J Biol Chem 274 (9), 5542-5549 (1999)
   PUBMED   10026169
REFERENCE   8  (bases 1 to 2666)
  AUTHORS   Tu,Y., Li,F. and Wu,C.
  TITLE     Nck-2, a novel Src homology2/3-containing adaptor protein that
            interacts with the LIM-only protein PINCH and components of growth
            factor receptor kinase-signaling pathways
  JOURNAL   Mol Biol Cell 9 (12), 3367-3382 (1998)
   PUBMED   9843575
REFERENCE   9  (bases 1 to 2666)
  AUTHORS   Chen,M., She,H., Davis,E.M., Spicer,C.M., Kim,L., Ren,R., Le
            Beau,M.M. and Li,W.
  TITLE     Identification of Nck family genes, chromosomal localization,
            expression, and signaling specificity
  JOURNAL   J Biol Chem 273 (39), 25171-25178 (1998)
   PUBMED   9737977
REFERENCE   10 (bases 1 to 2666)
  AUTHORS   Li,W., Hu,P., Skolnik,E.Y., Ullrich,A. and Schlessinger,J.
  TITLE     The SH2 and SH3 domain-containing Nck protein is oncogenic and a
            common target for phosphorylation by different surface receptors
  JOURNAL   Mol Cell Biol 12 (12), 5824-5833 (1992)
   PUBMED   1333047
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010978.8, BC007195.1 and
            AC009505.3.
            
            On Nov 22, 2018 this sequence version replaced NM_003581.4.
            
            Summary: This gene encodes a member of the NCK family of adaptor
            proteins. The protein contains three SH3 domains and one SH2
            domain. The protein has no known catalytic function but has been
            shown to bind and recruit various proteins involved in the
            regulation of receptor protein tyrosine kinases. It is through
            these regulatory activities that this protein is believed to be
            involved in cytoskeletal reorganization. Alternate transcriptional
            splice variants, encoding different isoforms, have been
            characterized. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (A). Variants 1 and 2
            both encode isoform A.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1092049.1,
                                           SRR1803611.114455.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000233154.9/ ENSP00000233154.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC010978.8         16166-16392
            228-370             AC010978.8         87684-87826
            371-2135            BC007195.1         1-1765
            2136-2666           AC009505.3         26412-26942
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q12.2"
     gene            1..2666
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /note="NCK adaptor protein 2"
                     /db_xref="GeneID:8440"
                     /db_xref="HGNC:HGNC:7665"
                     /db_xref="MIM:604930"
     exon            1..227
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            228..411
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            412..653
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             428..1570
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /note="isoform A is encoded by transcript variant 1;
                     SH2/SH3 adaptor protein NCK-beta; noncatalytic region of
                     tyrosine kinase, beta; cytoplasmic protein NCK2; growth
                     factor receptor-bound protein 4"
                     /codon_start=1
                     /product="cytoplasmic protein NCK2 isoform A"
                     /protein_id="NP_003572.2"
                     /db_xref="CCDS:CCDS33266.1"
                     /db_xref="GeneID:8440"
                     /db_xref="HGNC:HGNC:7665"
                     /db_xref="MIM:604930"
                     /translation="MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNA
                     ANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPAN
                     GSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWF
                     PSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEK
                     GETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSS
                     SGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFK
                     VQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ"
     exon            654..1375
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..2666
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2644..2649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /note="hexamer: AATAAA"
     polyA_site      2666
                     /gene="NCK2"
                     /gene_synonym="GRB4; NCKbeta"
                     /note="major polyA site"
ORIGIN      
        1 aggccggagc cgcgcggccg agcgggcggc gggcggaggg gagggcttgg cggccgggag
       61 gctcgcggcg cccgggccgg cagggtccgc ccgggccggc agcgtccgcc cggcggcggg
      121 aggagggagc ggcgcagaca aagagcggcg cctgggcggg cgcagcgcgg ccaccgcccc
      181 gggacccgcg ccgctgccct ccggctccgc gggcggccca cggcgagatt tcatgtgttc
      241 tttgtataca agcgacgtcc cagattataa ttctctgctg agatttgagt tggatttgag
      301 gatttggaga atccctgcag ctttgtaact tcagaggtgt aattagctga aaacatcatc
      361 gttttgaaga gttctgcgtt ttgccagtca cctctcaact gtgtgccaaa gaaggactcc
      421 atgaaagatg acagaagaag ttattgtgat agccaagtgg gactacaccg cccagcagga
      481 ccaggagctg gacatcaaga agaacgagcg gctgtggttg ctggacgact ccaagacgtg
      541 gtggcgggtg aggaacgcgg ccaacaggac gggctatgta ccgtccaact acgtggagcg
      601 gaagaacagc ctgaagaagg gctccctcgt gaagaacctg aaggacacac taggcctcgg
      661 caagacgcgc aggaagacca gcgcgcggga tgcgtccccc acgcccagca cggacgccga
      721 gtaccccgcc aatggcagcg gcgccgaccg catctacgac ctcaacatcc cggccttcgt
      781 caagttcgcc tatgtggccg agcgggagga tgagttgtcc ctggtgaagg ggtcgcgcgt
      841 caccgtcatg gagaagtgca gcgacggttg gtggcggggc agctacaacg ggcagatcgg
      901 ctggttcccc tccaactacg tcttggagga ggtggacgag gcggctgcgg agtccccaag
      961 cttcctgagc ctgcgcaagg gcgcctcgct gagcaatggc cagggctccc gcgtgctgca
     1021 tgtggtccag acgctgtacc ccttcagctc agtcaccgag gaggagctca acttcgagaa
     1081 gggggagacc atggaggtga ttgagaagcc ggagaacgac cccgagtggt ggaaatgcaa
     1141 aaatgcccgg ggccaggtgg gcctcgtccc caaaaactac gtggtggtcc tcagtgacgg
     1201 gcctgccctg caccctgcgc acgccccaca gataagctac accgggccct cgtccagcgg
     1261 gcgcttcgcg ggcagagagt ggtactacgg gaacgtgacg cggcaccagg ccgagtgcgc
     1321 cctcaacgag cggggcgtgg agggcgactt cctcattagg gacagcgagt cctcgcccag
     1381 cgacttctcc gtgtccctta aagcgtcagg gaagaacaaa cacttcaagg tgcagctcgt
     1441 ggacaatgtc tactgcattg ggcagcggcg cttccacacc atggacgagc tggtggaaca
     1501 ctacaaaaag gcgcccatct tcaccagcga gcacggggag aagctctacc tcgtcagggc
     1561 cctgcagtga cggcgccccg gccccacact cgcctcccgg gccccacggt ggagctgccc
     1621 gcccggcctt gtggcagagg ctcctcccgc ggggacggcc ccgacggctt ctctgcgagt
     1681 ctctctttat gttcaggtcg cttggtcggt tcgtctccca tttgccatcc aggcctcaca
     1741 cccacactcg agcccacccg gccggccagc tttagaggag gggaggagca gggcgagttc
     1801 acattattcc ttttccatcg gaagtggcgc tcgtgcattc aactcgttcc cgctcatgga
     1861 acccctcttt aaaaagacgc agggcacctg tgagcgcagg agcgagccta aggccaccca
     1921 gcggcagcgc ccgtgtcctg ggcactcagc gtgctgggca gagcaggtgc gatggcccca
     1981 gtcctagcag ccctcgccca tgtcctgtgc ccttacatgg ctcccggact gtgcagggag
     2041 ccgatacgtt tgctgatagc aatactggaa ccaccgggtg cgatggcagt gaggagactg
     2101 cccagtgcct ttggggctgt gcttgcaata aagaatttcc tggaaaggca gtctgcaaaa
     2161 gagggaaccg gtgactcaga aagacagatg ttttggtaat ttaccccaaa tgtgccatcc
     2221 acatagtgct ttttcctctt gcccttcggc ttgtttgaat ctcacaatta tgtatttaat
     2281 tctcaaagaa atatgtatct gtagccgttt gttgacacta atacagatga ttaaggaaaa
     2341 cagctgatct ttggggaagg gagctaccaa cactttatac acacacacac gtgcacacac
     2401 acacacacac acactatata tatatatatt atttacaggg aaatttttca gggtttacaa
     2461 aagagtatgt gattggtagt aagagacaca cagaatgttt atgaagaaat tgcattttct
     2521 ttttccttta catttgaact tctttatagt ttaaatataa cgtcttgaga tggcacattc
     2581 ctacgattga agaaggggtc ttgagatccc ctaaacttgc atacccagtt ttttggatat
     2641 tgtaataaaa aaaagtatta tgacaa
//
