LOCUS       NM_001291999            4451 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens NCK adaptor protein 1 (NCK1), transcript variant 3,
            mRNA.
ACCESSION   NM_001291999 XM_005247498
VERSION     NM_001291999.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Deng,S., Zhang,Y., Wang,H., Liang,W., Xie,L., Li,N., Fang,Y.,
            Wang,Y., Liu,J., Chi,H., Sun,Y., Ye,R., Shan,L., Shi,J., Shen,Z.,
            Wang,Y., Wang,S., Brosseau,J.P., Wang,F., Liu,G., Quan,Y. and Xu,J.
  TITLE     ITPRIPL1 binds CD3epsilon to impede T cell activation and enable
            tumor immune evasion
  JOURNAL   Cell 187 (9), 2305-2323 (2024)
   PUBMED   38614099
REFERENCE   3  (bases 1 to 4451)
  AUTHORS   He,P., Liu,Z., Qi,J., Shan,J. and Sheng,J.
  TITLE     Long noncoding RNA LINC00885 upregulates NCK1 to promote cell
            viability and migration of triple-negative breast cancer cells
            through sponging miR-654-3p
  JOURNAL   Cancer Biomark 39 (1), 63-78 (2024)
   PUBMED   37694355
  REMARK    GeneRIF: Long noncoding RNA LINC00885 upregulates NCK1 to promote
            cell viability and migration of triple-negative breast cancer cells
            through sponging miR-654-3p.
REFERENCE   4  (bases 1 to 4451)
  AUTHORS   Nuiyen,A., Rattanasri,A., Wipa,P., Roytrakul,S.,
            Wangteeraprasert,A., Pongcharoen,S. and Ngoenkam,J.
  TITLE     Lack of Nck1 protein and Nck-CD3 interaction caused the increment
            of lipid content in Jurkat T cells
  JOURNAL   BMC Mol Cell Biol 23 (1), 36 (2022)
   PUBMED   35902806
  REMARK    GeneRIF: Lack of Nck1 protein and Nck-CD3 interaction caused the
            increment of lipid content in Jurkat T cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4451)
  AUTHORS   Salokas,K., Liu,X., Ohman,T., Chowdhury,I., Gawriyski,L.,
            Keskitalo,S. and Varjosalo,M.
  TITLE     Physical and functional interactome atlas of human receptor
            tyrosine kinases
  JOURNAL   EMBO Rep 23 (6), e54041 (2022)
   PUBMED   35384245
REFERENCE   6  (bases 1 to 4451)
  AUTHORS   Rivero-Lezcano,O.M., Marcilla,A., Sameshima,J.H. and Robbins,K.C.
  TITLE     Wiskott-Aldrich syndrome protein physically associates with Nck
            through Src homology 3 domains
  JOURNAL   Mol Cell Biol 15 (10), 5725-5731 (1995)
   PUBMED   7565724
REFERENCE   7  (bases 1 to 4451)
  AUTHORS   Rivero-Lezcano,O.M., Sameshima,J.H., Marcilla,A. and Robbins,K.C.
  TITLE     Physical association between Src homology 3 elements and the
            protein product of the c-cbl proto-oncogene
  JOURNAL   J Biol Chem 269 (26), 17363-17366 (1994)
   PUBMED   7517397
REFERENCE   8  (bases 1 to 4451)
  AUTHORS   Meisenhelder,J. and Hunter,T.
  TITLE     The SH2/SH3 domain-containing protein Nck is recognized by certain
            anti-phospholipase C-gamma 1 monoclonal antibodies, and its
            phosphorylation on tyrosine is stimulated by platelet-derived
            growth factor and epidermal growth factor treatment
  JOURNAL   Mol Cell Biol 12 (12), 5843-5856 (1992)
   PUBMED   1448108
REFERENCE   9  (bases 1 to 4451)
  AUTHORS   Park,D. and Rhee,S.G.
  TITLE     Phosphorylation of Nck in response to a variety of receptors,
            phorbol myristate acetate, and cyclic AMP
  JOURNAL   Mol Cell Biol 12 (12), 5816-5823 (1992)
   PUBMED   1333046
REFERENCE   10 (bases 1 to 4451)
  AUTHORS   Lehmann,J.M., Riethmuller,G. and Johnson,J.P.
  TITLE     Nck, a melanoma cDNA encoding a cytoplasmic protein consisting of
            the src homology units SH2 and SH3
  JOURNAL   Nucleic Acids Res 18 (4), 1048 (1990)
   PUBMED   2107526
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA855124.1, BC006403.2 and
            AC011597.27.
            
            On Nov 23, 2018 this sequence version replaced NM_001291999.1.
            
            Summary: The protein encoded by this gene is one of the signaling
            and transforming proteins containing Src homology 2 and 3 (SH2 and
            SH3) domains. It is located in the cytoplasm and is an adaptor
            protein involved in transducing signals from receptor tyrosine
            kinases to downstream signal recipients such as RAS. Alternatively
            spliced transcript variants encoding different isoforms have been
            found. [provided by RefSeq, Jun 2010].
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants 1 and 3 encode the same protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.254690.1,
                                           SRR1660807.77761.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000481752.6/ ENSP00000417273.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-595               DA855124.1         1-595
            596-1971            BC006403.2         539-1914
            1972-4451           AC011597.27        75634-78113         c
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.3"
     gene            1..4451
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /note="NCK adaptor protein 1"
                     /db_xref="GeneID:4690"
                     /db_xref="HGNC:HGNC:7664"
                     /db_xref="MIM:600508"
     exon            1..146
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            147..390
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..1298
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /note="isoform 1 is encoded by transcript variant 3; NCK
                     tyrosine kinase; non-catalytic region of tyrosine kinase;
                     SH2/SH3 adaptor protein NCK-alpha; melanoma NCK protein;
                     cytoplasmic protein NCK1; NCK adapter protein 1; SH2/SH3
                     adapter protein NCK-alpha; SH2/SH3 adapter protein NCK1"
                     /codon_start=1
                     /product="SH2/SH3 adapter protein NCK1 isoform 1"
                     /protein_id="NP_001278928.1"
                     /db_xref="CCDS:CCDS3092.1"
                     /db_xref="GeneID:4690"
                     /db_xref="HGNC:HGNC:7664"
                     /db_xref="MIM:600508"
                     /translation="MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNS
                     MNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGE
                     RLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYV
                     TEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMD
                     VIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGK
                     FAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQL
                     KETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS"
     exon            391..1103
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..4451
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1946..1951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /note="hexamer: AATAAA"
     polyA_site      1971
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /note="major polyA site"
     regulatory      3011..3016
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /note="hexamer: AGTAAA"
     polyA_site      3038
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
     regulatory      4423..4428
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
                     /note="hexamer: AATAAA"
     polyA_site      4451
                     /gene="NCK1"
                     /gene_synonym="NCK; nck-1; NCKalpha"
ORIGIN      
        1 accgcgcgtg ccccgcctct ctcccaagag ctacgcggcg gcggcggagc gcaggcctcg
       61 tgccgttacg gccatcacgg cggccgcagt ggcgtcctgg agccctcctc aggtgagctg
      121 gagctgcccg gctcggcagc gggaagtgct gaagctgctg aaagatggca gaagaagtgg
      181 tggtagtagc caaatttgat tatgtggccc aacaagaaca agagttggac atcaagaaga
      241 atgagagatt atggcttctg gatgattcta agtcctggtg gcgagttcga aattccatga
      301 ataaaacagg ttttgtgcct tctaactatg tggaaaggaa aaacagtgct cggaaagcat
      361 ctattgtgaa aaacctaaag gataccttag gcattggaaa agtgaaaaga aaacctagtg
      421 tgccagattc tgcatctcct gctgatgata gttttgttga cccaggggaa cgtctctatg
      481 acctcaacat gcccgcttat gtgaaattta actacatggc tgagagagag gatgaattat
      541 cattgataaa ggggacaaag gtgatcgtca tggagaaatg cagtgatggg tggtggcgtg
      601 gtagctacaa tggacaagtt ggatggttcc cttcaaacta tgtaactgaa gaaggtgaca
      661 gtcctttggg tgaccatgtg ggttctctgt cagagaaatt agcagcagtc gtcaataacc
      721 taaatactgg gcaagtgttg catgtggtac aggctcttta cccattcagc tcatctaatg
      781 atgaagaact taatttcgag aaaggagatg taatggatgt tattgaaaaa cctgaaaatg
      841 acccagagtg gtggaaatgc aggaagatca atggtatggt tggtctagta ccaaaaaact
      901 atgttaccgt tatgcagaat aatccattaa cttcaggttt ggaaccatca cctccacagt
      961 gtgattacat taggccttca ctcactggaa agtttgctgg caatccttgg tattatggca
     1021 aagtcaccag gcatcaagca gaaatggcat taaatgaaag aggacatgaa ggggatttcc
     1081 tcattcgtga tagtgaatct tcgccaaatg atttctcagt atcactaaaa gcacaaggga
     1141 aaaacaagca ttttaaagtc caactaaaag agactgtcta ctgcattggg cagcgtaaat
     1201 tcagcaccat ggaagaactt gtagaacatt acaaaaaggc accaattttt acaagtgaac
     1261 aaggagaaaa attatatctt gtcaagcatt tatcatgata ctgctgacca gaagtgactg
     1321 ctgtgtagct gtaatttgtc atgtaattga agactgagaa aatgttgggt ccagtcgtgc
     1381 ttgattggaa attgttgttt ctaaatctat atgagaattg acaataagta tttttattat
     1441 aactcagccc atacatatat actatgtatg cagtgcatct gcatagaaca gttccttatc
     1501 cttggccttc tgttttattg tttttttctt tgctgttttc cctttgcttc taatattaca
     1561 gttttgtatt ttgtaaacaa aaatcaaata atgcatatca gaatctttat atggaagaaa
     1621 tcctttattg cctttccttt gtttccttgt aaaggcaccc tgttctgtta tggtttttca
     1681 ttatataaaa ttattatatc tatatatgac atatgctaaa atttcttgga gagtgttaat
     1741 cttttctgtg actaaatagc aataataagt ggaaaattag aaattatttc caggtattat
     1801 atttgtcaca ggccattgta aataccaagt atattgtgtc tgccataatt tttaaaaata
     1861 cattcattgt cttcagtcat acagcaagac acatgagaca tagattagaa aacatgttgt
     1921 acaattttaa tttacaactg ttggaaataa aaatcactta atttttttcc agtgcttctc
     1981 cctcatctgg ttattcaaga aaacaaaaca gtctctgaga tatttagctt ttcaaactgt
     2041 aaatagatgc tctagtgtta tttatttttt taatcccact tgtattattt tacctctaga
     2101 gcatcttgta ttaggacatg ttatatttat gccagtggga aataagttat ggccaagttt
     2161 tgcaaaaaca ggaagcagtg agatacttgt ttttttctcc tcactaaata tcagtaattg
     2221 tcaggaatgg tattacctat tttcatttcc tcttttcagc tttaagtttt gttgattggg
     2281 acactaaaac tgatgtatac ctgaggaaaa aatagaatgt gctcatggtt agggaaaatt
     2341 atattatttt taatttttta atttgttttt taaccaagta ggaatttggg tgtatggata
     2401 agaggccaat ctgcttctgt aggctataga agaaaaccag ttgtatttta tggtcataat
     2461 ttgtccccct attattctgg agatttaaat ttaagtaaag tagccatcag tattttaatt
     2521 gaaaaatact aaagccacat aactgagtag gatgtcgtat gatactttca acaaattccc
     2581 taatgctgcc taagcatatg gatgttttaa ttctttgctg ttataaatag atatctaata
     2641 cgccagaatt actgatggta aaattgaagt tgtgtaactt gtgaattttt tttatcaatg
     2701 ttttaaatat tatttaaatc ttaaaatcat ttaaagactt atgttgccac tgggtggcag
     2761 ccatggcttc tcccactaag caacagaagt gaccaaatat aattaagaca aattttcatc
     2821 tctattacat ctgcttttaa aacattttag ttcatattct attatttttc taactcagta
     2881 ctcctttgtt gagggtaatt attagtagtt aagtttttaa tggaaataaa aatcatgtat
     2941 gcttatcttt gctgtaaaat caaatgaata taaattgagg aaaactttaa attgttgtag
     3001 gatgactagt agtaaacatc atcttaattt ttaacatata tttgatgatg ctttcctttt
     3061 ctctgtatgt ttctgggttt tcccccagtc ttccttatga aaaaaatgta tgtttgtaaa
     3121 aagagaacaa ttaatgcttt gcatcacaga cataaactat tcatagtaaa ctgcactgag
     3181 ctgatattaa tagagcagac ttttgagaaa gtaggagtgt agtagcactg gaaacagaat
     3241 gcaaatttct gttccataat taggtttatt gtagctatag cgctcccaga taacccatgt
     3301 gtgctagtaa tatctattgt gtgctcttac tatggtataa aaacctcgct ggaagctact
     3361 tattttgtac ttaaatattt taccaatttt taaaaagtct taatccaatg cttataccag
     3421 tgactgtttt tataatactt gaagtaactt taagaactga aacaaccctg gaaaactcta
     3481 catagctaga tagtaacaat acttgtataa gaaaccaagt ttccttattc ccagcctggt
     3541 gtctgtatcg tgacacacta ctctgtgtat gcttggtttt agactaggag gagagtcctg
     3601 ggaagggcaa tattacagaa gttttcttcc acatgaggag aatgcttagt ttcaaattta
     3661 aatacatgtg tcagatggaa ccctgcctaa ctcacttcta caaataactt ccccaaaatc
     3721 ttagaaggtt ttttagaaca tgatataagc tttgtgacaa aaacaaatgc cattgtgtcc
     3781 cttatttagt cataaaacct taggattcag ctgaatatat attcacacat gtatattaca
     3841 catatatcac tatacagtta acacatgcac cttgcactat tagtgacaaa taatactgct
     3901 aaacaaaata ggcttcatgc aatctattgc cattgctaga agaataaata ggcctagcag
     3961 atgggcaact tgggttgtta gaataaggtc attcttgcag aatatggcac ttaaattatc
     4021 tccagctgta atgtcctacc atataatcca gaggttgcat agaagcagag ttgtcacagt
     4081 ctctgtagca cgtttgtgag aattctctag gcaggcccag cccttctcta gaagtttgaa
     4141 tttcactccc accttgttgc tggggtacct tcttcagggt acatctaaac acagtggcct
     4201 tatacagaaa tgttgttcaa cccacacagt gctttaaaaa attaattgcc aacatttaaa
     4261 aatcagggag tttcacataa aattcagact tcttgtttcc ctttaaaaat gagaagctaa
     4321 taacatgagt tgaattccca caagtcaaca atgagctgga atctaatatg gatggtcctc
     4381 ttatctttac cagtgcacat ggtgaaaaaa ttcaaacagt ataataaagt cttctttcaa
     4441 gtcaaaatat a
//
