LOCUS       NM_013416               1623 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript
            variant 2, mRNA.
ACCESSION   NM_013416
VERSION     NM_013416.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Li,L., Mao,R., Yuan,S., Xie,Q., Meng,J., Gu,Y., Tan,S., Xu,X.,
            Gao,C., Liu,H., Ma,C., Man,S.M., Meng,X., Xu,T. and Qi,X.
  TITLE     NCF4 attenuates colorectal cancer progression by modulating
            inflammasome activation and immune surveillance
  JOURNAL   Nat Commun 15 (1), 5170 (2024)
   PUBMED   38886341
  REMARK    GeneRIF: NCF4 attenuates colorectal cancer progression by
            modulating inflammasome activation and immune surveillance.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Liu,X., Shi,Y., Liu,R., Song,K. and Chen,L.
  TITLE     Structure of human phagocyte NADPH oxidase in the activated state
  JOURNAL   Nature 627 (8002), 189-195 (2024)
   PUBMED   38355798
REFERENCE   3  (bases 1 to 1623)
  AUTHORS   Azarova,Y.E., Klyosova,E.Y., Ivakin,V.E., Churilin,M.I.,
            Kolomoets,I.I., Sunyaykina,O.A., Ragulina,V.A. and Polonikov,A.V.
  TITLE     Polymorphisms of the NCF4 Gene Increase the Risk of Chronic Heart
            Failure in Patients with Type 2 Diabetes Mellitus
  JOURNAL   Bull Exp Biol Med 176 (1), 77-81 (2023)
   PUBMED   38085396
  REMARK    GeneRIF: Polymorphisms of the NCF4 Gene Increase the Risk of
            Chronic Heart Failure in Patients with Type 2 Diabetes Mellitus.
REFERENCE   4  (bases 1 to 1623)
  AUTHORS   Chen,Y., He,F., Wang,R., Yao,M., Li,Y., Guo,D. and He,S.
  TITLE     NCF1/2/4 Are Prognostic Biomarkers Related to the Immune
            Infiltration of Kidney Renal Clear Cell Carcinoma
  JOURNAL   Biomed Res Int 2021, 5954036 (2021)
   PUBMED   34708124
  REMARK    GeneRIF: NCF1/2/4 Are Prognostic Biomarkers Related to the Immune
            Infiltration of Kidney Renal Clear Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1623)
  AUTHORS   Zhang,T.P., Li,R., Huang,Q., Pan,H.F., Ye,D.Q. and Li,X.M.
  TITLE     Association of NCF2, NCF4, and CYBA Gene Polymorphisms with
            Rheumatoid Arthritis in a Chinese Population
  JOURNAL   J Immunol Res 2020, 8528976 (2020)
   PUBMED   33145364
  REMARK    GeneRIF: Association of NCF2, NCF4, and CYBA Gene Polymorphisms
            with Rheumatoid Arthritis in a Chinese Population.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1623)
  AUTHORS   Leto,T.L., Adams,A.G. and de Mendez,I.
  TITLE     Assembly of the phagocyte NADPH oxidase: binding of Src homology 3
            domains to proline-rich targets
  JOURNAL   Proc Natl Acad Sci U S A 91 (22), 10650-10654 (1994)
   PUBMED   7938008
REFERENCE   7  (bases 1 to 1623)
  AUTHORS   Tsunawaki,S., Mizunari,H., Nagata,M., Tatsuzawa,O. and Kuratsuji,T.
  TITLE     A novel cytosolic component, p40phox, of respiratory burst oxidase
            associates with p67phox and is absent in patients with chronic
            granulomatous disease who lack p67phox
  JOURNAL   Biochem Biophys Res Commun 199 (3), 1378-1387 (1994)
   PUBMED   8147882
REFERENCE   8  (bases 1 to 1623)
  AUTHORS   Wientjes,F.B., Hsuan,J.J., Totty,N.F. and Segal,A.W.
  TITLE     p40phox, a third cytosolic component of the activation complex of
            the NADPH oxidase to contain src homology 3 domains
  JOURNAL   Biochem J 296 (Pt 3) (Pt 3), 557-561 (1993)
   PUBMED   8280052
REFERENCE   9  (bases 1 to 1623)
  AUTHORS   Leiding,J.W. and Holland,S.M.
  TITLE     Chronic Granulomatous Disease
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   22876374
REFERENCE   10 (bases 1 to 1623)
  AUTHORS   Jones,J.H.
  TITLE     The essence of operating room nursing. Paramedical personnel
  JOURNAL   Australas Nurses J 7 (1), 44-45 (1977)
   PUBMED   243433
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC002798.1 and AK290924.1.
            
            On May 21, 2020 this sequence version replaced NM_013416.3.
            
            Summary: The protein encoded by this gene is a cytosolic regulatory
            component of the superoxide-producing phagocyte NADPH-oxidase, a
            multicomponent enzyme system important for host defense. This
            protein is preferentially expressed in cells of myeloid lineage. It
            interacts primarily with neutrophil cytosolic factor 2
            (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor
            1 (NCF1/p47-phox), which further interacts with the small G protein
            RAC1 and translocates to the membrane upon cell stimulation. This
            complex then activates flavocytochrome b, the membrane-integrated
            catalytic core of the enzyme system. The PX domain of this protein
            can bind phospholipid products of the PI(3) kinase, which suggests
            its role in PI(3) kinase-mediated signaling events. The
            phosphorylation of this protein was found to negatively regulate
            the enzyme activity. Alternatively spliced transcript variants
            encoding distinct isoforms have been observed. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2). This isoform (2)
            lacks the PC (Phox and Cdc24p) motif, which may be important for
            the interaction with NCF2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002798.1, AK290924.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               BC002798.1         19-147
            130-1564            AK290924.1         70-1504
            1565-1623           BC002798.1         1583-1641
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.3"
     gene            1..1623
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /note="neutrophil cytosolic factor 4"
                     /db_xref="GeneID:4689"
                     /db_xref="HGNC:HGNC:7662"
                     /db_xref="MIM:601488"
     exon            1..198
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1213
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     neutrophil NADPH oxidase factor 4; neutrophil cytosol
                     factor 4; NCF-4; p40-phox; SH3 and PX domain-containing
                     protein 4; neutrophil cytosolic factor 4, 40kDa"
                     /codon_start=1
                     /product="neutrophil cytosol factor 4 isoform 2"
                     /protein_id="NP_038202.2"
                     /db_xref="CCDS:CCDS13935.1"
                     /db_xref="GeneID:4689"
                     /db_xref="HGNC:HGNC:7662"
                     /db_xref="MIM:601488"
                     /translation="MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIE
                     VKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIA
                     EMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSV
                     SPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGI
                     FPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKSVAWEGGACPAFLPSLRPLPLTS
                     PSHGSLSHSKAPSGSQMSHNAVTSHQRPGWPGQPHSPFPHPTPHFQPDASLLQPVTPL
                     GTSRWRKISAALPY"
     exon            199..283
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            284..437
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            438..508
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            509..636
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            637..694
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            695..793
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            794..1235
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1623
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1599..1604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /note="hexamer: AATAAA"
     polyA_site      1623
                     /gene="NCF4"
                     /gene_synonym="CGD3; NCF; P40PHOX; SH3PXD4"
                     /note="major polyA site"
ORIGIN      
        1 agactggaga gaagcaggcc tgagcctccc caaaggcagc tcctggggac tcccaggacc
       61 acaggctgag acgagacgca gggtggctgg aggaagtgag aggtgaactc agcctgggac
      121 tggctgggcg agactctcca cctgctccct gggaccatcg cccaccatgg ctgtggccca
      181 gcagctgcgg gccgagagtg actttgaaca gcttccggat gatgttgcca tctcggccaa
      241 cattgctgac atcgaggaga agagaggctt caccagccac tttgttttcg tcatcgaggt
      301 gaagacaaaa ggaggatcca agtacctcat ctaccgccgc taccgccagt tccatgcttt
      361 gcagagcaag ctggaggagc gcttcgggcc agacagcaag agcagtgccc tggcctgtac
      421 cctgcccaca ctcccagcca aagtctacgt gggtgtgaaa caggagatcg ccgagatgcg
      481 gatacctgcc ctcaacgcct acatgaagag cctgctcagc ctgccggtct gggtgctgat
      541 ggatgaggac gtccggatct tcttttacca gtcgccctat gactcagagc aggtgcccca
      601 ggcactccgc cggctccgcc cgcgcacccg gaaagtcaag agcgtgtccc cacagggcaa
      661 cagcgttgac cgcatggcag ctccgagagc agaggctcta tttgacttca ctggaaacag
      721 caaactggag ctgaatttca aagctggaga tgtgatcttc ctcctcagtc ggatcaacaa
      781 agactggctg gagggcactg tccggggagc cacgggcatc ttccctctct ccttcgtgaa
      841 gatcctcaaa gacttccctg aggaggacga ccccaccaac tggctgcgtt gctactacta
      901 cgaagacacc atcagcacca tcaagtctgt ggcctgggag ggaggggcct gtccagcctt
      961 cctgccatcc ctacgaccac tgcccctcac atcaccttct catgggtccc tctcccactc
     1021 caaagccccc agtggctccc agatgagcca caatgctgta acaagccatc aacgtccagg
     1081 gtggcctggc cagcctcatt cccctttccc ccaccccaca ccccacttcc agcctgatgc
     1141 ctccttactc cagcctgtca cccccttagg gacatcgcgg tggaggaaga tctcagcagc
     1201 actcccctat tgaaagacct gctggagctc acaaggcggg agttccagag agaggacata
     1261 gctctgaatt accgggacgc tgagggggat ctggttcggc tgctgtcgga tgaggacgta
     1321 gcgctcatgg tgcggcaggc tcgtggcctc ccctcccaga agcgcctctt cccctggaag
     1381 ctgcacatca cgcagaagga caactacagg gtctacaaca cgatgccatg agctgacggt
     1441 gtccctggag cagtgagggg acaccagcaa aaaccttcag ctctcagagg agattgggac
     1501 caggaaaacc tgggaggatg ggcagacttc ctgtctttga ggctaatgga cccgtggggc
     1561 ttgtaatctg tctctttcta ctatttacat ctgatttaaa taaaccattc catctgaaag
     1621 ggg
//
