LOCUS       NM_181351               5819 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript
            variant 2, mRNA.
ACCESSION   NM_181351
VERSION     NM_181351.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Martinez,A.L., Shannon,M.J., Sloan,T. and Mace,E.M.
  TITLE     CD56/NCAM mediates cell migration of human NK cells by promoting
            integrin-mediated adhesion turnover
  JOURNAL   Mol Biol Cell 35 (5), ar64 (2024)
   PUBMED   38507235
  REMARK    GeneRIF: CD56/NCAM mediates cell migration of human NK cells by
            promoting integrin-mediated adhesion turnover.
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Li,S., Zheng,S., Huang,X., Zhang,W., Liu,F. and Cao,Q.
  TITLE     Expression and Clinical Significance of CD30 and CD56 in
            Lymphoblastic Lymphoma: A Retrospective Analysis on
            Paraffin-Embedded Tissues by Immunohistochemistry
  JOURNAL   Fetal Pediatr Pathol 43 (2), 111-122 (2024)
   PUBMED   38213180
  REMARK    GeneRIF: Expression and Clinical Significance of CD30 and CD56 in
            Lymphoblastic Lymphoma: A Retrospective Analysis on
            Paraffin-Embedded Tissues by Immunohistochemistry.
REFERENCE   3  (bases 1 to 5819)
  AUTHORS   Yirmibes,S. and Balaban Adim,S.
  TITLE     A Comparative Study of CD56 and Smooth Muscle Actin Expression in
            Basal and Squamous Cell Carcinomas
  JOURNAL   Am J Dermatopathol 45 (12), 816-819 (2023)
   PUBMED   37982465
  REMARK    GeneRIF: A Comparative Study of CD56 and Smooth Muscle Actin
            Expression in Basal and Squamous Cell Carcinomas.
REFERENCE   4  (bases 1 to 5819)
  AUTHORS   Matejova,J., Fecskeova,L.K., Slovinska,L., Harvanova,D., Spakova,T.
            and Bzdilova,J.
  TITLE     Plasma-derived extracellular vesicle surface markers CD45, CD326
            and CD56 correlate with the stage of osteoarthritis: a primary
            study of a novel and promising diagnostic tool of the disease
  JOURNAL   Sci Rep 13 (1), 20071 (2023)
   PUBMED   37973964
  REMARK    GeneRIF: Plasma-derived extracellular vesicle surface markers CD45,
            CD326 and CD56 correlate with the stage of osteoarthritis: a
            primary study of a novel and promising diagnostic tool of the
            disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5819)
  AUTHORS   Darka Aslan,I., Sel,G., Barut,F., Baser Acikgoz,R., Balci,S.,
            Ozmen,U., Barut,A., Harma,M. and Harma,M.I.
  TITLE     Investigation of CD56, ADAM17 and FGF21 Expressions in the
            Placentas of Preeclampsia Cases
  JOURNAL   Medicina (Kaunas) 59 (6), 1145 (2023)
   PUBMED   37374349
  REMARK    GeneRIF: Investigation of CD56, ADAM17 and FGF21 Expressions in the
            Placentas of Preeclampsia Cases.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5819)
  AUTHORS   Rossell,R.J., Malik,A., Helfrich,W., Kemshead,J.T. and Patel,K.
  TITLE     A discordance between the human muscle NCAM sequence and those seen
            in other NCAM cDNA clones
  JOURNAL   Biochim Biophys Acta 1130 (1), 95-96 (1992)
   PUBMED   1543753
REFERENCE   7  (bases 1 to 5819)
  AUTHORS   van Duijnhoven,H.L., Helfrich,W., de Leij,L., Roebroek,A.J., van de
            Ven,W.J., Healey,K., Culverwell,A., Rossell,R.J., Kemshead,J.T. and
            Patel,K.
  TITLE     Splicing of the VASE exon of neural cell adhesion molecule (NCAM)
            in human small-cell lung carcinoma (SCLC)
  JOURNAL   Int J Cancer 50 (1), 118-123 (1992)
   PUBMED   1339414
REFERENCE   8  (bases 1 to 5819)
  AUTHORS   Lanier,L.L., Chang,C., Azuma,M., Ruitenberg,J.J., Hemperly,J.J. and
            Phillips,J.H.
  TITLE     Molecular and functional analysis of human natural killer
            cell-associated neural cell adhesion molecule (N-CAM/CD56)
  JOURNAL   J Immunol 146 (12), 4421-4426 (1991)
   PUBMED   1710251
REFERENCE   9  (bases 1 to 5819)
  AUTHORS   Bello,M.J., Salagnon,N., Rey,J.A., Guichaoua,M.R.,
            Berge-Lefranc,J.L., Jordan,B.R. and Luciani,J.M.
  TITLE     Precise in situ localization of NCAM, ETS1, and D11S29 on human
            meiotic chromosomes
  JOURNAL   Cytogenet Cell Genet 52 (1-2), 7-10 (1989)
   PUBMED   2612216
REFERENCE   10 (bases 1 to 5819)
  AUTHORS   Dickson,G., Gower,H.J., Barton,C.H., Prentice,H.M., Elsom,V.L.,
            Moore,S.E., Cox,R.D., Quinn,C., Putt,W. and Walsh,F.S.
  TITLE     Human muscle neural cell adhesion molecule (N-CAM): identification
            of a muscle-specific sequence in the extracellular domain
  JOURNAL   Cell 50 (7), 1119-1130 (1987)
   PUBMED   2887295
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA337649.1, BC047244.1,
            S71824.1, BC014205.2, AK054570.1 and AW131780.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_181351.4.
            
            Summary: This gene encodes a cell adhesion protein which is a
            member of the immunoglobulin superfamily. The encoded protein is
            involved in cell-to-cell interactions as well as cell-matrix
            interactions during development and differentiation. The encoded
            protein plays a role in the development of the nervous system by
            regulating neurogenesis, neurite outgrowth, and cell migration.
            This protein is also involved in the expansion of T lymphocytes, B
            lymphocytes and natural killer (NK) cells which play an important
            role in immune surveillance. This protein plays a role in signal
            transduction by interacting with fibroblast growth factor
            receptors, N-cadherin and other components of the extracellular
            matrix and by triggering signalling cascades involving FYN-focal
            adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and
            phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this
            gene, cell surface molecule CD56, plays a role in several
            myeloproliferative disorders such as acute myeloid leukemia and
            differential expression of this gene is associated with
            differential disease progression. For example, increased expression
            of CD56 is correlated with lower survival in acute myeloid leukemia
            patients whereas increased severity of COVID-19 is correlated with
            decreased abundance of CD56-expressing NK cells in peripheral
            blood. Alternative splicing results in multiple transcript variants
            encoding distinct protein isoforms. [provided by RefSeq, Aug 2020].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region compared to variant 5. The resulting
            protein (isoform 2) is shorter compared to isoform 5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC047244.1, AK292453.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000316851.12/ ENSP00000318472.8
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-245               DA337649.1         148-392
            246-1842            BC047244.1         231-1827
            1843-2860           S71824.1           1821-2838
            2861-3982           BC047244.1         2846-3967
            3983-5253           BC014205.2         3020-4290
            5254-5805           AK054570.1         844-1395
            5806-5819           AW131780.1         16-29               c
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.2"
     gene            1..5819
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /note="neural cell adhesion molecule 1"
                     /db_xref="GeneID:4684"
                     /db_xref="HGNC:HGNC:7656"
                     /db_xref="MIM:116930"
     exon            1..245
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..2770
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; antigen recognized by monoclonal antibody
                     5.1H11; neural cell adhesion molecule, NCAM"
                     /codon_start=1
                     /product="neural cell adhesion molecule 1 isoform 2
                     precursor"
                     /protein_id="NP_851996.2"
                     /db_xref="CCDS:CCDS73385.1"
                     /db_xref="GeneID:4684"
                     /db_xref="HGNC:HGNC:7656"
                     /db_xref="MIM:116930"
                     /translation="MLQTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVA
                     GDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGED
                     GSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVIL
                     KKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQ
                     NIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKK
                     VDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGD
                     PIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGE
                     YICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFR
                     DGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQAD
                     TPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEAS
                     MEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVQGEPSAPKLEGQMGED
                     GNSIKVNLIKQDDGGSPIRHYLVRYRALSSEWKPEIRLPSGSDHVMLKSLDWNAEYEV
                     YVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTSGLSTGAIVGILIVIFVLLLVVVD
                     ITCYFLNKCGLFMCIAVNLCGKAGPGAKGKDMEEGKAAFSKDESKEPIVEVRTEEERT
                     PNHDGGKHTEPNETTPLTEPEKGPVEAKPECQETETKPAPAEVKTVPNDATQTKENES
                     KA"
     exon            246..320
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            321..539
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            540..683
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            684..821
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            822..939
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1109
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1252
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1282
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1433
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1618
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1715
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1886
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..2018
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2021
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2146
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2324
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2532
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2649
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     exon            2650..5819
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3963..3968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /note="hexamer: AATAAA"
     polyA_site      3982
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /note="major polyA site"
     regulatory      5795..5800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /note="hexamer: AATAAA"
     polyA_site      5819
                     /gene="NCAM1"
                     /gene_synonym="CD56; MSK39; NCAM"
                     /note="major polyA site"
ORIGIN      
        1 actcattctc cgatcagcgc gtgaacgcag ctcggctgcc gctggcagga aacaattctg
       61 caaaaataat catactcagc ctggcaattg tctgccccta ggtctgtcgc tcagccgccg
      121 tccacactcg ctgcaggggg gggggcacag aatttaccgc ggcaagaaca tccctcccag
      181 ccagcagatt acaatgctgc aaactaagga tctcatctgg actttgtttt tcctgggaac
      241 tgcagtttct ctgcaggtgg atattgttcc cagccagggg gagatcagcg ttggagagtc
      301 caaattcttc ttatgccaag tggcaggaga tgccaaagat aaagacatct cctggttctc
      361 ccccaatgga gaaaagctca ccccaaacca gcagcggatc tcagtggtgt ggaatgatga
      421 ttcctcctcc accctcacca tctataacgc caacatcgac gacgccggca tttacaagtg
      481 tgtggttaca ggcgaggatg gcagtgagtc agaggccacc gtcaacgtga agatctttca
      541 gaagctcatg ttcaagaatg cgccaacccc acaggagttc cgggaggggg aagatgccgt
      601 gattgtgtgt gatgtggtca gctccctccc accaaccatc atctggaaac acaaaggccg
      661 agatgtcatc ctgaaaaaag atgtccgatt catagtcctg tccaacaact acctgcagat
      721 ccggggcatc aagaaaacag atgagggcac ttatcgctgt gagggcagaa tcctggcacg
      781 gggggagatc aacttcaagg acattcaggt cattgtgaat gtgccaccta ccatccaggc
      841 caggcagaat attgtgaatg ccaccgccaa cctcggccag tccgtcaccc tggtgtgcga
      901 tgccgaaggc ttcccagagc ccaccatgag ctggacaaag gatggggaac agatagagca
      961 agaggaagac gatgagaagt acatcttcag cgacgatagt tcccagctga ccatcaaaaa
     1021 ggtggataag aacgacgagg ctgagtacat ctgcattgct gagaacaagg ctggcgagca
     1081 ggatgcgacc atccacctca aagtctttgc aaaacccaaa atcacatatg tagagaacca
     1141 gactgccatg gaattagagg agcaggtcac tcttacctgt gaagcctccg gagaccccat
     1201 tccctccatc acctggagga cttctacccg gaacatcagc agcgaagaaa aggcttcgtg
     1261 gactcgacca gagaagcaag agactctgga tgggcacatg gtggtgcgta gccatgcccg
     1321 tgtgtcgtcg ctgaccctga agagcatcca gtacactgat gccggagagt acatctgcac
     1381 cgccagcaac accatcggcc aggactccca gtccatgtac cttgaagtgc aatatgcccc
     1441 aaagctacag ggccctgtgg ctgtgtacac ttgggagggg aaccaggtga acatcacctg
     1501 cgaggtattt gcctatccca gtgccacgat ctcatggttt cgggatggcc agctgctgcc
     1561 aagctccaat tacagcaata tcaagatcta caacaccccc tctgccagct atctggaggt
     1621 gaccccagac tctgagaatg attttgggaa ctacaactgt actgcagtga accgcattgg
     1681 gcaggagtcc ttggaattca tccttgttca agcagacacc ccctcttcac catccatcga
     1741 ccaggtggag ccatactcca gcacagccca ggtgcagttt gatgaaccag aggccacagg
     1801 tggggtgccc atcctcaaat acaaagctga gtggagagca gttggtgaag aagtatggca
     1861 ttccaagtgg tatgatgcca aggaagccag catggagggc atcgtcacca tcgtgggcct
     1921 gaagcccgaa acaacgtacg ccgtaaggct ggcggcgctc aatggcaaag ggctgggtga
     1981 gatcagcgcg gcctccgagt tcaagacgca gccagtccaa ggggaaccca gtgcacctaa
     2041 gctcgaaggg cagatgggag aggatggaaa ctctattaaa gtgaacctga tcaagcagga
     2101 tgacggcggc tcccccatca gacactatct ggtcaggtac cgagcgctct cctccgagtg
     2161 gaaaccagag atcaggctcc cgtctggcag tgaccacgtc atgctgaagt ccctggactg
     2221 gaatgctgag tatgaggtct acgtggtggc tgagaaccag caaggaaaat ccaaggcggc
     2281 tcattttgtg ttcaggacct cggcccagcc cacagccatc ccagccaacg gcagccccac
     2341 ctcaggcctg agcaccgggg ccatcgtggg catcctcatc gtcatcttcg tcctgctcct
     2401 ggtggttgtg gacatcacct gctacttcct gaacaagtgt ggcctgttca tgtgcattgc
     2461 ggtcaacctg tgtggaaaag ccgggcccgg ggccaagggc aaggacatgg aggagggcaa
     2521 ggccgccttc tcgaaagatg agtccaagga gcccatcgtg gaggttcgaa cggaggagga
     2581 gaggacccca aaccatgatg gagggaaaca cacagagccc aacgagacca cgccactgac
     2641 ggagcccgag aagggccccg tagaagcaaa gccagagtgc caggagacag aaacgaagcc
     2701 agcgccagcc gaagtcaaga cggtccccaa tgacgccaca cagacaaagg agaacgagag
     2761 caaagcatga tgggtgaaga gaaccgagca aagatcaaaa taaaaagtga cacagcagct
     2821 tcaccagagc atttccaaca ccacagacac acacacgcac gcacacacac aaacacacat
     2881 gcacacacac acatctcatt tctctagtgt cttttgcctt taaaaaaaac taaacagata
     2941 aaacatggga atctcctttt tgtaggttta tagaaagggt ccctttgttg cacactcact
     3001 tgtaagaaaa tgagacaaaa aggttaaacc cacagccaaa ctaggacact ccgttccctg
     3061 aaaccgttaa aaaatcaaac aaaaggaccc caaattaaga atctaggaag ctcagaaacg
     3121 aaatctaggt tcaggaagac cacacttggt gttacccgat tggcacagac cagtttcaga
     3181 gaaatacttt caggcactaa gactaatcga atgaacaaag tccacagttt atttttatac
     3241 tttcagtcaa gtttgaactc tgtaaaacct cataaataag ttataatttc tgttcacttt
     3301 gtatttgttc agtatgcaaa gtgtgtcacc ctttctagct gaattcaatt cccacgtaga
     3361 ctcttatttt ataggacgaa tgccaaattg cagcttctgg gggtagatct caatttgcag
     3421 tattcagact tctttttctt tcttttacat tcttttttct ttctttcttt ctgccaactt
     3481 tgttttccag tgtttacaag gtgacaaatg tttgactttg gttgtgttta aatgtccgtg
     3541 taaaatagct gccttttatt ttttaaggta acaaatacca cctagaggta ggtaggatca
     3601 tcccacgctt gctttagcac aggacaactt tacaaaacat gattgtttac agctgctctt
     3661 cccctctttt ctgatctgca gtttttgcct gggtcccact caggtgaaaa tccatctcat
     3721 tctggaatgg ttttgctttt gaatttttgg ttatttttgt gtttctttgg gggttagacc
     3781 actttctgat tagccgccac ctgcctgcat ctgtgaaaag ggatctgctc ccaggcgttc
     3841 tcacccttct tttgaaggac tccttaggct ttgttgaatg aagcagagaa gattgtatag
     3901 ttggggctgg tcttggtgaa cacacattat taccccacac atcccctttg tgtagaaagc
     3961 caaataaaat ctatacatac catttccttt tgagcccaga atctagattt gagcggaaga
     4021 gcatgtgtgc ttcagggaat tagtgtcttt ttttggaaat ctgttgaagt aaagtaacat
     4081 cggccttctg ttcacttagg cagcatttat agaaacaaaa gaagaaagaa acaacctact
     4141 gtctggagtc ataacacaac tttcctggat tggaaaccaa gtgggggaaa aaatacagaa
     4201 actttaaggg ggatgggagg ggggggagaa gggaaaagcc agccctttgt atagaaattt
     4261 tgcttttttt tccctcattc tactttagaa ctgcaagctt gtgcactgtg gatgcgtgaa
     4321 tattttagtg tgaaacgtgt ttttgtcata gtattgaaat aaaacttcaa catagtttgg
     4381 ttgtggaagg tatagcagat agttcagaaa aaatattcag gaaacaaaaa tcactcaaac
     4441 ggaatcgaag ccttttaaca aagaaaatga aatacagatg atgatgatga tgatgaagat
     4501 gatgctaagt aaacagaaat cagtactccg catgcgctcc tctcctaagg tacaaagcag
     4561 caagaggtta gggtggcaag gctgcctctg ggtccattct gtgggccact ctccccaacg
     4621 ttctgacact tctgcagtct gatcagtggc gatgctagat tataatttca aactgtgaag
     4681 aataatggtc ttgtcatttg ctcaatgtgg ggttatgttg cattttctca gctcctgggg
     4741 atggaaatgg aggatcccag aacacacagc cctggcccct ttgattctag ggcctgcaca
     4801 gatctctggt tcaaatgcac aggccctcag aatagaggaa catgaagaga gatcttagag
     4861 cacacagtag aatgtgagag cctgggtgtc tgagaccggg agggcccagc agtgaggggc
     4921 aggctcttct ggtcaccagg ctgttcagtg gactcagttc ttcatcttgt aatgtcgatg
     4981 gctttgccac accaggccaa gcccatgcca taccttgtca agactgtcaa agtggttgtg
     5041 gttaggtcaa actggttttg gttctgatgg ttaggaagaa acaggtcagc cctcagatca
     5101 cctggcccgg gacagctgac cccctagaac cctggctctg ccattagcta ggacctaaga
     5161 ctctgcccac attttggtct gttctctccc attacacata ggtttgtctc agcatgcaag
     5221 agtttttcct ttaaaaaaaa aaaaaaaaaa aaaaaaaaag caatgctttc tctaaaatca
     5281 aagagggagt cattttattc caagatgttt tatcttttat gttaagagat caaagcttat
     5341 aattttcttt tttaattttt gaaggaggga tcaactccag tttccaatgt ctatgtgtct
     5401 atgtgtgtat gtgccataca tatgtattca catgaagacc ggcatggcca agttctgctg
     5461 gaggagcact caagtgtgac gagcagggcc actggaccct gcagggctgt ggtgtatata
     5521 gtgcagcttt ggaggtggaa ctctattttc acacttttct atggagcctt ccgagtccca
     5581 ggttttcact tgaggctgtc tgtctggatg gcggttttca gacctccatt aacatcccta
     5641 cccagcattc tgtacttcgg gggccttctc tcttgttata aaacttttta ccaagtgaaa
     5701 catcgatacc acctttgttt ccattctcac tggtgtaaat actgagtact aactgagaat
     5761 tttgactttg cattctgtcg gaatacttgt gttcaataaa aattgaaaga aaaaagcta
//
