LOCUS       NM_032041               3476 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens neurocalcin delta (NCALD), transcript variant 8, mRNA.
ACCESSION   NM_032041
VERSION     NM_032041.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Dong,C., Yin,F., Zhu,D., Cai,X., Chen,C. and Liu,X.
  TITLE     NCALD affects drug resistance and prognosis by acting as a ceRNA of
            CX3CL1 in ovarian cancer
  JOURNAL   J Cell Biochem 121 (11), 4470-4483 (2020)
   PUBMED   32030795
  REMARK    GeneRIF: NCALD affects drug resistance and prognosis by acting as a
            ceRNA of CX3CL1 in ovarian cancer.
REFERENCE   3  (bases 1 to 3476)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3476)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3476)
  AUTHORS   Feng,L.Y. and Li,L.
  TITLE     Low expression of NCALD is associated with chemotherapy resistance
            and poor prognosis in epithelial ovarian cancer
  JOURNAL   J Ovarian Res 13 (1), 35 (2020)
   PUBMED   32228639
  REMARK    GeneRIF: Low expression of NCALD is associated with chemotherapy
            resistance and poor prognosis in epithelial ovarian cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3476)
  AUTHORS   O'Callaghan,D.W., Ivings,L., Weiss,J.L., Ashby,M.C., Tepikin,A.V.
            and Burgoyne,R.D.
  TITLE     Differential use of myristoyl groups on neuronal calcium sensor
            proteins as a determinant of spatio-temporal aspects of Ca2+ signal
            transduction
  JOURNAL   J Biol Chem 277 (16), 14227-14237 (2002)
   PUBMED   11836243
REFERENCE   7  (bases 1 to 3476)
  AUTHORS   Wang,W., Zhou,Z., Zhao,W., Huang,Y., Tang,R., Ying,K., Xie,Y. and
            Mao,Y.
  TITLE     Molecular cloning, mapping and characterization of the human
            neurocalcin delta gene (NCALD)
  JOURNAL   Biochim Biophys Acta 1518 (1-2), 162-167 (2001)
   PUBMED   11267673
REFERENCE   8  (bases 1 to 3476)
  AUTHORS   Burgoyne,R.D. and Weiss,J.L.
  TITLE     The neuronal calcium sensor family of Ca2+-binding proteins
  JOURNAL   Biochem J 353 (Pt 1), 1-12 (2001)
   PUBMED   11115393
  REMARK    Review article
            Erratum:[Biochem J 2001 Mar 15;354(Pt 3):727]
REFERENCE   9  (bases 1 to 3476)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 3476)
  AUTHORS   Ladant,D.
  TITLE     Calcium and membrane binding properties of bovine neurocalcin delta
            expressed in Escherichia coli
  JOURNAL   J Biol Chem 270 (7), 3179-3185 (1995)
   PUBMED   7852401
  REMARK    GeneRIF: The author characterizes the biochemical properties of
            recombinant bovine neurocalcin delta, whose protein sequence is
            identical to the human ortholog
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA211806.1, AL136612.1,
            AP000426.3, BC063428.1 and BM681731.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032041.2.
            
            Summary: This gene encodes a member of the neuronal calcium sensor
            (NCS) family of calcium-binding proteins. The protein contains an
            N-terminal myristoylation signal and four EF-hand calcium binding
            loops. The protein is cytosolic at resting calcium levels; however,
            elevated intracellular calcium levels induce a conformational
            change that exposes the myristoyl group, resulting in protein
            association with membranes and partial co-localization with the
            perinuclear trans-golgi network. The protein is thought to be a
            regulator of G protein-coupled receptor signal transduction.
            Several alternatively spliced variants of this gene have been
            determined, all of which encode the same protein; additional
            variants may exist but their biological validity has not been
            determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (8) differs in the 5' UTR compared
            to variant 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.118176.1,
                                           SRR1803615.97054.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220931.11/ ENSP00000220931.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-332               DA211806.1         243-574
            333-494             AL136612.1         326-487
            495-495             AP000426.3         58058-58058
            496-859             AL136612.1         489-852
            860-1108            BC063428.1         1026-1274
            1109-3038           AP000426.3         88411-90340
            3039-3476           BM681731.1         18-455              c
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q22.3"
     gene            1..3476
                     /gene="NCALD"
                     /note="neurocalcin delta"
                     /db_xref="GeneID:83988"
                     /db_xref="HGNC:HGNC:7655"
                     /db_xref="MIM:606722"
     exon            1..108
                     /gene="NCALD"
                     /inference="alignment:Splign:2.1.0"
     exon            109..505
                     /gene="NCALD"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..709
                     /gene="NCALD"
                     /codon_start=1
                     /product="neurocalcin-delta"
                     /protein_id="NP_114430.2"
                     /db_xref="CCDS:CCDS6292.1"
                     /db_xref="GeneID:83988"
                     /db_xref="HGNC:HGNC:7655"
                     /db_xref="MIM:606722"
                     /translation="MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLS
                     MEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLK
                     WAFSMYDLDGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNR
                     DGKLSLEEFIRGAKSDPSIVRLLQCDPSSAGQF"
     exon            506..611
                     /gene="NCALD"
                     /inference="alignment:Splign:2.1.0"
     exon            612..3476
                     /gene="NCALD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3203..3208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCALD"
                     /note="hexamer: AATAAA"
     polyA_site      3230
                     /gene="NCALD"
     regulatory      3257..3262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCALD"
                     /note="hexamer: AATAAA"
     polyA_site      3279
                     /gene="NCALD"
                     /note="major polyA site"
     regulatory      3449..3454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NCALD"
                     /note="hexamer: ATTAAA"
     polyA_site      3476
                     /gene="NCALD"
ORIGIN      
        1 aaaggcttgt gagaatctgg tggatgctgg accttgctgc tgctgctact gctgtttcca
       61 ggggctgcag agcatggact gttaaatctt gcacttcttc tgagtgagct gaattcttgc
      121 cgccaggatg gggaaacaga acagcaagct gcgcccggag gtcatgcagg acttgctgga
      181 aagcacagac tttacagagc atgagatcca ggaatggtat aaaggcttct tgagagactg
      241 ccccagtgga catttgtcaa tggaagagtt taagaaaata tatgggaact ttttccctta
      301 tggggatgct tccaaatttg cagagcatgt cttccgcacc ttcgatgcaa atggagatgg
      361 gacaatagac tttagagaat tcatcatcgc cttgagtgta acttcgaggg ggaagctgga
      421 gcagaagctg aaatgggcct tcagcatgta cgacctggac ggaaatggct atatcagcaa
      481 ggcagagatg ctagagatcg tgcaggcaat ctataagatg gtttcctctg taatgaaaat
      541 gcctgaagat gagtcaaccc cagagaaaag aacagaaaag atcttccgcc agatggacac
      601 caatagagac ggaaaactct ccctggaaga gttcatccga ggagccaaaa gcgacccgtc
      661 cattgtgcgc ctcctgcagt gcgacccgag cagtgccggc cagttctgag ccctgcgccc
      721 accaatcgaa ttgtagagct gcttgtgttc ccttttgatt cttcttttta acaatttttt
      781 tttttttttg ccaaacaata tcaatggtga tgccgtcccc tgtgcggtct gatgcgcctt
      841 cctccgtgac gccttcagct tcttttgtcg tggatgcttc gtgggaatgc ccagagcccc
      901 agtgtgcttg tggagagcat ggacagactt cgtggtgttc attgtttgat gatttttaat
      961 cgttactatt attttttttt attctaatgt ctctgttcta aaacgtaaga ctcgggggtt
     1021 ggggcaaaag aagggaaacc catccagtcc tgtgattcta ttgcaagctt caaggggctt
     1081 ttgtttgaaa gacaaaactc cccacctgaa tctgttgtca cacgtgccgt aggggtgatg
     1141 gatggcaccg gatgctggat tccccaagaa caagttaccc tctggggtga ggctattcca
     1201 gcgagctggg acatttcccc atgggggccc actcccctct cttccccagc aggctgtagt
     1261 ttctaagctg tgaacatttc aagataaatt aacagaggag aggaaaaaga tggctcagct
     1321 attttttcac aggtttacac tagttgagct aatatgcgtg tctttggaaa ttaaacacaa
     1381 atggtaacat attccaaaac cagacccatc ttgttgccta ttgtgataaa ataaaaagac
     1441 ggctgtatat aacatattgg gtaatgcaga ccaaattaag tgttttgcct tgtttaaatg
     1501 aaatgcatgt ttagtgagca ctaatacaat cttattccag aagactgttt ttagtagctt
     1561 attgtgaagt aagacaacta taatgaatgt ctgtcttgtt tggaagtcat atctgtcttt
     1621 gcacaaatgt accaatcgac aagtatattt tatatattcc ataaaaatac aaagtaaccc
     1681 tgactagggc ccaactttaa ttttgaatgc atttccagag tggccatgcc tagagggcag
     1741 atgcagagca ggtggtagtg ggacaggaca attggagcac aggaatgtta acatgtatga
     1801 caggggacca gtagggtggt ttccctctca ggcccagcag cccattgaca gcattagact
     1861 ggcggcatgg tgcttttctg agcagatcaa tactctgcag actcgaaaaa acatcacata
     1921 cattcttgga acttcccagt ggtttaatct atgtgcatgg ttagggagcc aggtctggaa
     1981 tattcagttt ccctgcccct gttaaagaat cagaggttgg gcagtcatca aattcatcat
     2041 aaagacatgg gcaagtgtgt ctgtggtttc caaggccccc ctatggagaa tccaaaagta
     2101 ttttccattg ccgtgctctt tgaatgcaga cttctatttc cagaagtgac agcacaagtc
     2161 tgagttgctg tttggtctgg tgacctcaga cacactaatt tgaattgaaa gctaagagta
     2221 aaaatttgct ggttacaggc gagtcatact cttgcaagta gttagcaaag ggaggcccaa
     2281 attctcaagg ttgttgatgg ggaacttgcc actaagagaa ggcagagagg tccctagtgg
     2341 gtatatttgc tgccaagcca cttgccaaag aagaggaacc acagaaagag agacatcatg
     2401 accaggagaa aaatgtgact agacatgcta acctccaggt ttttatatat gacttgagtc
     2461 tgctgtaatt ggcagcagaa atccaaattt gtatggtaga ccaaaaagaa ccaaatccat
     2521 agggtgaaat tttgagacct agactctgta aaaataatcc tagtcttcct ccaggggtca
     2581 gttcctcaca gtggttctgt accaaaactt gccaaattcc tccatggcca agtgttaaaa
     2641 tctgtgtttg gaaaatagcg aattaaccta agacacagaa ggcagactgg gtgaggagac
     2701 ctagcatgcc ctattggcag tgctcaggag ctgcatccca cttttccctg ctctgaatcg
     2761 aagtcctagt tccttccttt gattctcctt tggtaggtgg aatcagttaa tgttttgaga
     2821 aacctgcctg ggctctgccc ttagtcatga catctcgctg agccagaccc actctgttcc
     2881 ttggaaccta gagctggagt gaggagtaga ggtctccggc tattccagaa agaaaagtga
     2941 gccacatgca ggctgatgaa tgccgacact tccagaatgt atagaaatag tccctgtcct
     3001 ggcctgccac tgaccctgtc tgtattttct cggaggttgt ttttctcctt ctccttccca
     3061 ggaaggtctt tgtatgtcga atccagtgca ctcaagtttg gccaagggac tccacagcac
     3121 ccagaagact gcatgcctca aggtttatgt cactcctctg ctgggctgtt cattgtcatt
     3181 gctgtgttca gggacctttg gaaataaaac ctgttctgtc ccaaataaaa ccagcctgtg
     3241 atgttcaagg gactggaata aagtggctta cgacctgaag gattctacag agtgctcaac
     3301 tgttcataca tcattcagag tgggagggag ctgggggtgc tgtcccatcc catctgtatg
     3361 cccacggtgg atttaataat atataattta taaatcatag cctatggagt ggcccgtaaa
     3421 tcagttgact gtgtagctct tgcctggcat taaagcatgt ttttggttta acatga
//
