LOCUS       NM_001405699            6002 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 31, mRNA.
ACCESSION   NM_001405699
VERSION     NM_001405699.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6002)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6002)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6002)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6002)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6002)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6002)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6002)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6002)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405699.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-582             AC277909.1         203684-203766
            583-652             AC277909.1         204036-204105
            653-965             AC277909.1         204942-205254
            966-1120            AC277909.1         206382-206536
            1121-1330           AC277909.1         206639-206848
            1331-1403           AC277909.1         210898-210970
            1404-1615           AC277909.1         211434-211645
            1616-1825           AC277909.1         212992-213201
            1826-1928           AC254635.1         129707-129809
            1929-2143           AC254635.1         130645-130859
            2144-2216           AC254635.1         131910-131982
            2217-2428           AC254635.1         132446-132657
            2429-2638           AC254635.1         134007-134216
            2639-2741           AC254635.1         135990-136092
            2742-2956           AC254635.1         136928-137142
            2957-3029           AC254635.1         138180-138252
            3030-3235           AC254635.1         138717-138922
            3236-3287           AC254635.1         140214-140265
            3288-3451           AC254635.1         144172-144335
            3452-3503           AC254635.1         145378-145429
            3504-3676           AC254635.1         146069-146241
            3677-3728           AC254635.1         146961-147012
            3729-3901           AC254635.1         147615-147787
            3902-3953           AC254635.1         148497-148548
            3954-4126           AC254635.1         149171-149343
            4127-4238           AC254635.1         150058-150169
            4239-6002           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6002
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..582
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            583..652
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            653..965
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1120
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1330
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             1156..4482
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 10 is encoded by transcript variant 31;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 10"
                     /protein_id="NP_001392628.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKNDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECA
                     ITCSNSHGPCDSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSS
                     ATNVSMVVSAGPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLAN
                     QQNKYKYEECKDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLRE
                     KLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDED
                     EDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNI
                     NITFEEDKVNSTLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAE
                     MNILEINEKLHPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLR
                     NERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQAL
                     LTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAP
                     REMPKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSH
                     VEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEML
                     ASYQSYSGTFHSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQ
                     DSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSC
                     PRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDV
                     DRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYG
                     SSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLS
                     GVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVDNRF
                     LTLMGTSLHLVFQMGVIFPQ"
     exon            1331..1403
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1615
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1825
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1928
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2143
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2216
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2428
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2638
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2639..2741
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2742..2956
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3029
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3235
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3236..3287
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3288..3451
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3452..3503
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..3676
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3677..3728
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3729..3901
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..3953
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4126
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4127..4238
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4239..6002
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5978..5983
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      5998
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacagg aagatggcca atctttccta agctgctcac cttacaagaa
      541 aacgaatcgt actgctaaga attcaaactt cagcagtcat ggggagcctt ggaaggagcc
      601 cgaatcactg atggaattgg acagtgcatg gagatggttc agcaggacaa ggtgctgaaa
      661 actcatcccc gtgtgacctc aagtaagcca tgtaactctg tgaacctgca gttttatcat
      721 ttttaaaata aagaaacatg acagattttc attatgacac agaatgtcag gtctcccaga
      781 tgccagaaaa tacatttact taaagccgtt gatacgtctt aaagcggttt ccttacagtg
      841 tcattggagg acagtgtgga gtgcagagag acatgctttg aaatgggatt gatccagtcc
      901 tccttccttc actaccacat gaatgctggg cagcccaggg tcaacccacc gcaccctcaa
      961 ctcagacggt tacctggcac gctggccaca gtctacctca ctcttatcag agtctgagct
     1021 actggcagtg ctttcagctc tgagttcagg cacctcgaac cttgtttttg tggtgaagga
     1081 tcctaaagtg ctgtggggag tgatcacatt tttcacaaca tccctggctc cacctcttct
     1141 gccacaaacg tcagcatggt ggtatcagct ggcccttggt ccagcgagaa ggcagagacg
     1201 aacattttag aaatcaacga gaaattgcgc ccccagctgg cagagaagaa acagcagttc
     1261 agaaacctca aagagaaatg ttttgtaact caactggccg gcttcctggc caaccgacag
     1321 aagaaataca aaaatgacga agatgaggat gaagatgttc aagttgagga ggctgagaaa
     1381 gtactggaat catctgcccc cagggaggtg cagaaggctg aagaaagcaa agtccctgag
     1441 gactcactgg aggaatgtgc catcacttgt tcaaatagcc acggcccttg tgactccaac
     1501 cagcctcaca agaacatcaa catcaccttt gaggaagaca aagtcaactc agctctggtt
     1561 gtagacagag aatcctctca tgatgaatgt caggatgctg taaacattct cccagtccct
     1621 ggccccacct cttctgccac aaacgtcagc atggtggtat cagccggccc tttgttcagc
     1681 gagaaggcag agatgaacat tctagaaatc aacgagaaat tgcatcccca gctggcagag
     1741 aagaatcagc agttcagaaa cctcaaagag aaatgttttg taactcaact ggcctgcttc
     1801 ctggccaacc agcagaacaa atacaaatat gaagagtgca aagacctcat aaaatctatg
     1861 ctgaggaaag agcgacagtt caaggaggag aagcttgcag agcagctcaa gcaagctgag
     1921 gagctcaggc aatataaagt cctggttcac tctcaggaac gagagctgac ccacttaagg
     1981 gagaagttac gggaagggag agatgcctcc cgctcattga atcagcatct ccaggccctc
     2041 ctcactccgg ataagccaga caagtcccag gggcaggacc tccaagaaca gctggctgag
     2101 gggtgtagac tggcacagca acttttccag aagctcagcc cagaaaatga cgaagatgag
     2161 gatgaagatg ttcaagttga ggaggctgag aaagtactgg aatcatctgc ccccagggag
     2221 gtgcagaagg ctgaagaaag caaagtccct gaggactcac tggaggaatg tgccatcact
     2281 tgttcaaata gccacagccc ttgtgactcc aaccagcctc acaagaacat caacatcacc
     2341 tttgaggaag acaaagtcaa ctcaactctg gttgtagaca gagaatcctc tcatgatgaa
     2401 tgtcaggatg ctgtaaacat tctcccagtc cctggcccca cctcttctgc cacaaacgtc
     2461 agcatggtgg tatcagccgg ccccttgtcc agcgagaagg cagagatgaa cattctagaa
     2521 atcaacgaga aattgcatcc ccagctggca gagaagaaac agcagttcag aaacctcaaa
     2581 gagaaatgtt ttgtaactca actggcctgc ttcctggcca accagcagaa caaatacaaa
     2641 tatgaagagt gcaaagacct cataaaatct atgctgagga atgagcgaca gttcaaggag
     2701 gagaagcttg cagagcagct caagcaagct gaggagctca ggcaatataa agtcctggtt
     2761 cactctcagg aacgggagct gacccagtta agggagaagt tacgggaagg gagagatgcc
     2821 tcccgctcat tgaatcagca tctccaggcc ctcctcactc cggatgagcc agacaagtcc
     2881 caggggcagg acctccaaga acagctggct gaggggtgta gactggcaca gcaccttgtc
     2941 caaaagctca gcccagaaaa tgacaatgat gacgatgaag atgttcaagt tgaggtggct
     3001 gagaaagtgc aggaatcgtc tgcccccagg gagatgccga aggctgaaga aaaggaagtc
     3061 cctgaggact cactggagga atgtgccatc acttgttcaa atagccatgg cccttatgac
     3121 tccaaccagc cacataggaa aaccaaaatc acatttgagg aagacaaagt cgactcaact
     3181 ctcattggct catcctctca tgttgaatgg gaggatgctg tacacattat cccagaaaat
     3241 gaaagtgatg atgaggaaga ggaagaaaaa gggccagtgt ctcccaggaa tctgcaggag
     3301 tctgaagagg aggaagtccc ccaggagtcc tgggatgaag gttattcgac tctctcaatt
     3361 cctcctgaaa tgttggcctc gtaccagtct tacagcggca catttcactc attagaggaa
     3421 cagcaagtct gcatggctgt tgacataggc ggacatcggt gggatcaagt gaaaaaggag
     3481 gaccaagagg caacaggtcc caggctcagc agggagctgc tggatgagaa agggcctgaa
     3541 gtcttgcagg actcactgga tagatgttat tcaactcctt caggttatct tgaactgact
     3601 gactcatgcc agccctacag aagtgccttt tacatattgg agcaacagcg tgttggctgg
     3661 gctcttgaca tggatgaaat tgaaaagtac caagaagtgg aagaagacca agacccatca
     3721 tgccccaggc tcagcaggga gctgctggat gagaaagagc ctgaagtctt gcaggactca
     3781 ctggatagat gttattcgac tccttcaggt tatcttgaac tgcctgactt aggccagccc
     3841 tacagaagtg ctgtttactc attggaggaa cagtaccttg gcttggctct tgacgtggac
     3901 agaattaaaa aggaccagga agaggaagaa gaccaaggcc caccatgccc caggctcagc
     3961 agggagctgc tggaggcagt agagcctgaa gtcttgcagg actcactgga tagatgttat
     4021 tcaactcctt ccagttgtct tgaacagcct gactcctgcc tgccctatgg aagttccttt
     4081 tatgcattgg aggaaaaaca tgttggcttt tctcttgacg tgggagaaat tgaaaagaag
     4141 gggaagggga agaaaagaag gggaagaaga tcaacgaaga aaagaaggag aaggggaaga
     4201 aaagaagggg aagaagatca aaacccacca tgccccaggc tcagcggtgt gctgatggaa
     4261 gtggaagagc ctgaagtctt acaggactca ctggatagat gttattcgac tccgtcaatg
     4321 ttctttgaac tacctgactc attccagcac tacagaagtg tgttttactc atttgaggaa
     4381 cagcacatca gcttcgccct tgacgtggac aataggtttc ttactttgat gggaacaagt
     4441 ctccacctgg tcttccagat gggagtcata ttcccacagt aagcagccct tactaagccg
     4501 agagatgtca ttcctgcagg caggacctat aggcacgtga agatttgaat gaaactatag
     4561 ttccatttgg aagcccagac ataggatggg tcagtgggca tggctctatt cctattctca
     4621 gaccatgcca gtggcaacct gtgctcagtc tgaagacaat ggacccaagt taggtgtgac
     4681 acgttcacat aactgtgcag cacatgccgg gagtgatcag tcagacattt taatttgaac
     4741 cacgtatctc tgggtagcta caaagttcct cagggatttc attttgcagg catgtctctg
     4801 agcttctata cctgctcaag gtcagtgtca tctttgtgtt tagctcatcc aaaggtgtta
     4861 ccctggtttc aatgaaccta acctcattct ttgtatcttc agtgttgaat tgttttagct
     4921 gatccatctt taacgcagga gggatccttg gctgaggatt gtatttcaga accaccaact
     4981 gctcttgaca attgttaacc cgctaggctc ctttggttag agaagccaca gtccttcagc
     5041 ctccaattgg tgtcagtact taggaagacc acagctagat ggacaaacag cattgggagg
     5101 ccttagccct gctcctctcg attccatcct gtagagaaca ggagtcagga gccgctggca
     5161 ggagacagca tgtcacccag gactctgccg gtgcagaata tgaacaacgc catgttcttg
     5221 cagaaaacgc ttagcctgag tttcatagga ggtaatcacc agacaactgc agaatgtaga
     5281 acactgagca ggacaactga cctgtctcct tcacatagtc catatcacca caaatcacac
     5341 aacaaaaagg agaagagata ttttgggttc aaaaaaagta aaaagataat atagctgcat
     5401 ttctttagtt attttgaacc ccaaatattt cctcatcttt ttgttgttgt cattgatggt
     5461 ggtgacatgg acttgtttat agaggacagg tcagctgtct ggctcaatga tctacattct
     5521 gaagttgtct gaaaatgtct tcatgattaa attcagccta aacgttttgc cgggaacact
     5581 gcagagacaa tgctgtgagt ttccaacctt agcccatctg cgggcagaga aggtctagtt
     5641 tgtccatcag cattatcatg atatcaggac tggttacttg gttaaggagg ggtctaggag
     5701 atctgtccct tttagagaca ccttacttat aatgaagtat ttgggagggt ggttttcaaa
     5761 agtagaaatg tcctgtattc cgatgatcat cctgtaaaca ttttatcatt tattaatcat
     5821 ccctgcctgt gtctattatt atattcatat ctctacgctg gaaactttct gcctcaatat
     5881 ttactgtgcc tttgtttttg ctagtttgtg ttgttgaaaa aaaaaacatt ctctgcctga
     5941 gttttaattt ttgtccaaag ttattttaat ctatacaatt aaaagctttt gcctatcact
     6001 ct
//
