LOCUS       NM_001405692            6037 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 24, mRNA.
ACCESSION   NM_001405692
VERSION     NM_001405692.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6037)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6037)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6037)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6037)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6037)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6037)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6037)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6037)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405692.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-582             AC277909.1         203684-203766
            583-652             AC277909.1         204036-204105
            653-682             AC277909.1         204942-204971
            683-837             AC277909.1         206382-206536
            838-1047            AC277909.1         206639-206848
            1048-1150           AC277909.1         208694-208796
            1151-1365           AC277909.1         209632-209846
            1366-1438           AC277909.1         210898-210970
            1439-1650           AC277909.1         211434-211645
            1651-1860           AC277909.1         212992-213201
            1861-1963           AC254635.1         129707-129809
            1964-2178           AC254635.1         130645-130859
            2179-2251           AC254635.1         131910-131982
            2252-2463           AC254635.1         132446-132657
            2464-2673           AC254635.1         134007-134216
            2674-2776           AC254635.1         135990-136092
            2777-2991           AC254635.1         136928-137142
            2992-3064           AC254635.1         138180-138252
            3065-3270           AC254635.1         138717-138922
            3271-3322           AC254635.1         140214-140265
            3323-3486           AC254635.1         144172-144335
            3487-3538           AC254635.1         145378-145429
            3539-3711           AC254635.1         146069-146241
            3712-3763           AC254635.1         146961-147012
            3764-3936           AC254635.1         147615-147787
            3937-3988           AC254635.1         148497-148548
            3989-4161           AC254635.1         149171-149343
            4162-4273           AC254635.1         150058-150169
            4274-6037           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6037
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..582
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            583..652
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            653..682
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            683..837
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1047
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             873..4517
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 7 is encoded by transcript variant 24;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 7"
                     /protein_id="NP_001392621.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPC
                     DSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLREKLREGRDASR
                     SLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEA
                     EKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVN
                     STLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKL
                     HPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKL
                     AEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDEPDKSQ
                     GQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAPREMPKAEEKE
                     VPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHII
                     PENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYQSYSGTF
                     HSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTP
                     SGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDE
                     KEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEE
                     EDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKH
                     VGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSGVLMEVEEPE
                     VLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVDNRFLTLMGTSLHL
                     VFQMGVIFPQ"
     exon            1048..1150
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1365
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1438
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1650
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1860
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..1963
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2178
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2251
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2252..2463
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2673
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2776
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2991
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2992..3064
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3270
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3322
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3323..3486
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..3538
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3711
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3712..3763
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3764..3936
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..3988
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3989..4161
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4162..4273
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4274..6037
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6013..6018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      6033
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacagg aagatggcca atctttccta agctgctcac cttacaagaa
      541 aacgaatcgt actgctaaga attcaaactt cagcagtcat ggggagcctt ggaaggagcc
      601 cgaatcactg atggaattgg acagtgcatg gagatggttc agcaggacaa ggtgctgaaa
      661 actcatcccc gtgtgacctc aaacggttac ctggcacgct ggccacagtc tacctcactc
      721 ttatcagagt ctgagctact ggcagtgctt tcagctctga gttcaggcac ctcgaacctt
      781 gtttttgtgg tgaaggatcc taaagtgctg tggggagtga tcacattttt cacaacatcc
      841 ctggctccac ctcttctgcc acaaacgtca gcatggtggt atcagctggc ccttggtcca
      901 gcgagaaggc agagacgaac attttagaaa tcaacgagaa attgcgcccc cagctggcag
      961 agaagaaaca gcagttcaga aacctcaaag agaaatgttt tgtaactcaa ctggccggct
     1021 tcctggccaa ccgacagaag aaatacaagt atgaagagtg taaagacctc ataaaattta
     1081 tgctgaggaa tgagcgacag ttcaaggagg agaagcttgc agagcagctc aagcaagctg
     1141 aggagctcag gcaatataaa gtcctggttc actctcagga acgggagctg acccagttaa
     1201 gggagaagtt acgggaaggg agagatgcct cccgctcatt gaatcagcat ctccaggccc
     1261 tcctcactcc ggataagcca gacaagtccc aggggcagga cctccaagaa cagctggctg
     1321 aggggtgtag actggcacag caacttttcc aaaagctcag cccagaaaat gacgaagatg
     1381 aggatgaaga tgttcaagtt gaggaggctg agaaagtact ggaatcatct gcccccaggg
     1441 aggtgcagaa ggctgaagaa agcaaagtcc ctgaggactc actggaggaa tgtgccatca
     1501 cttgttcaaa tagccacggc ccttgtgact ccaaccagcc tcacaagaac atcaacatca
     1561 cctttgagga agacaaagtc aactcagctc tggttgtaga cagagaatcc tctcatgatg
     1621 aatgtcagga tgctgtaaac attctcccag tccctggccc cacctcttct gccacaaacg
     1681 tcagcatggt ggtatcagcc ggccctttgt tcagcgagaa ggcagagatg aacattctag
     1741 aaatcaacga gaaattgcat ccccagctgg cagagaagaa tcagcagttc agaaacctca
     1801 aagagaaatg ttttgtaact caactggcct gcttcctggc caaccagcag aacaaataca
     1861 aatatgaaga gtgcaaagac ctcataaaat ctatgctgag gaaagagcga cagttcaagg
     1921 aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat aaagtcctgg
     1981 ttcactctca ggaacgagag ctgacccact taagggagaa gttacgggaa gggagagatg
     2041 cctcccgctc attgaatcag catctccagg ccctcctcac tccggataag ccagacaagt
     2101 cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactggca cagcaacttt
     2161 tccagaagct cagcccagaa aatgacgaag atgaggatga agatgttcaa gttgaggagg
     2221 ctgagaaagt actggaatca tctgccccca gggaggtgca gaaggctgaa gaaagcaaag
     2281 tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccac agcccttgtg
     2341 actccaacca gcctcacaag aacatcaaca tcacctttga ggaagacaaa gtcaactcaa
     2401 ctctggttgt agacagagaa tcctctcatg atgaatgtca ggatgctgta aacattctcc
     2461 cagtccctgg ccccacctct tctgccacaa acgtcagcat ggtggtatca gccggcccct
     2521 tgtccagcga gaaggcagag atgaacattc tagaaatcaa cgagaaattg catccccagc
     2581 tggcagagaa gaaacagcag ttcagaaacc tcaaagagaa atgttttgta actcaactgg
     2641 cctgcttcct ggccaaccag cagaacaaat acaaatatga agagtgcaaa gacctcataa
     2701 aatctatgct gaggaatgag cgacagttca aggaggagaa gcttgcagag cagctcaagc
     2761 aagctgagga gctcaggcaa tataaagtcc tggttcactc tcaggaacgg gagctgaccc
     2821 agttaaggga gaagttacgg gaagggagag atgcctcccg ctcattgaat cagcatctcc
     2881 aggccctcct cactccggat gagccagaca agtcccaggg gcaggacctc caagaacagc
     2941 tggctgaggg gtgtagactg gcacagcacc ttgtccaaaa gctcagccca gaaaatgaca
     3001 atgatgacga tgaagatgtt caagttgagg tggctgagaa agtgcaggaa tcgtctgccc
     3061 ccagggagat gccgaaggct gaagaaaagg aagtccctga ggactcactg gaggaatgtg
     3121 ccatcacttg ttcaaatagc catggccctt atgactccaa ccagccacat aggaaaacca
     3181 aaatcacatt tgaggaagac aaagtcgact caactctcat tggctcatcc tctcatgttg
     3241 aatgggagga tgctgtacac attatcccag aaaatgaaag tgatgatgag gaagaggaag
     3301 aaaaagggcc agtgtctccc aggaatctgc aggagtctga agaggaggaa gtcccccagg
     3361 agtcctggga tgaaggttat tcgactctct caattcctcc tgaaatgttg gcctcgtacc
     3421 agtcttacag cggcacattt cactcattag aggaacagca agtctgcatg gctgttgaca
     3481 taggcggaca tcggtgggat caagtgaaaa aggaggacca agaggcaaca ggtcccaggc
     3541 tcagcaggga gctgctggat gagaaagggc ctgaagtctt gcaggactca ctggatagat
     3601 gttattcaac tccttcaggt tatcttgaac tgactgactc atgccagccc tacagaagtg
     3661 ccttttacat attggagcaa cagcgtgttg gctgggctct tgacatggat gaaattgaaa
     3721 agtaccaaga agtggaagaa gaccaagacc catcatgccc caggctcagc agggagctgc
     3781 tggatgagaa agagcctgaa gtcttgcagg actcactgga tagatgttat tcgactcctt
     3841 caggttatct tgaactgcct gacttaggcc agccctacag aagtgctgtt tactcattgg
     3901 aggaacagta ccttggcttg gctcttgacg tggacagaat taaaaaggac caggaagagg
     3961 aagaagacca aggcccacca tgccccaggc tcagcaggga gctgctggag gcagtagagc
     4021 ctgaagtctt gcaggactca ctggatagat gttattcaac tccttccagt tgtcttgaac
     4081 agcctgactc ctgcctgccc tatggaagtt ccttttatgc attggaggaa aaacatgttg
     4141 gcttttctct tgacgtggga gaaattgaaa agaaggggaa ggggaagaaa agaaggggaa
     4201 gaagatcaac gaagaaaaga aggagaaggg gaagaaaaga aggggaagaa gatcaaaacc
     4261 caccatgccc caggctcagc ggtgtgctga tggaagtgga agagcctgaa gtcttacagg
     4321 actcactgga tagatgttat tcgactccgt caatgttctt tgaactacct gactcattcc
     4381 agcactacag aagtgtgttt tactcatttg aggaacagca catcagcttc gcccttgacg
     4441 tggacaatag gtttcttact ttgatgggaa caagtctcca cctggtcttc cagatgggag
     4501 tcatattccc acagtaagca gcccttacta agccgagaga tgtcattcct gcaggcagga
     4561 cctataggca cgtgaagatt tgaatgaaac tatagttcca tttggaagcc cagacatagg
     4621 atgggtcagt gggcatggct ctattcctat tctcagacca tgccagtggc aacctgtgct
     4681 cagtctgaag acaatggacc caagttaggt gtgacacgtt cacataactg tgcagcacat
     4741 gccgggagtg atcagtcaga cattttaatt tgaaccacgt atctctgggt agctacaaag
     4801 ttcctcaggg atttcatttt gcaggcatgt ctctgagctt ctatacctgc tcaaggtcag
     4861 tgtcatcttt gtgtttagct catccaaagg tgttaccctg gtttcaatga acctaacctc
     4921 attctttgta tcttcagtgt tgaattgttt tagctgatcc atctttaacg caggagggat
     4981 ccttggctga ggattgtatt tcagaaccac caactgctct tgacaattgt taacccgcta
     5041 ggctcctttg gttagagaag ccacagtcct tcagcctcca attggtgtca gtacttagga
     5101 agaccacagc tagatggaca aacagcattg ggaggcctta gccctgctcc tctcgattcc
     5161 atcctgtaga gaacaggagt caggagccgc tggcaggaga cagcatgtca cccaggactc
     5221 tgccggtgca gaatatgaac aacgccatgt tcttgcagaa aacgcttagc ctgagtttca
     5281 taggaggtaa tcaccagaca actgcagaat gtagaacact gagcaggaca actgacctgt
     5341 ctccttcaca tagtccatat caccacaaat cacacaacaa aaaggagaag agatattttg
     5401 ggttcaaaaa aagtaaaaag ataatatagc tgcatttctt tagttatttt gaaccccaaa
     5461 tatttcctca tctttttgtt gttgtcattg atggtggtga catggacttg tttatagagg
     5521 acaggtcagc tgtctggctc aatgatctac attctgaagt tgtctgaaaa tgtcttcatg
     5581 attaaattca gcctaaacgt tttgccggga acactgcaga gacaatgctg tgagtttcca
     5641 accttagccc atctgcgggc agagaaggtc tagtttgtcc atcagcatta tcatgatatc
     5701 aggactggtt acttggttaa ggaggggtct aggagatctg tcccttttag agacacctta
     5761 cttataatga agtatttggg agggtggttt tcaaaagtag aaatgtcctg tattccgatg
     5821 atcatcctgt aaacatttta tcatttatta atcatccctg cctgtgtcta ttattatatt
     5881 catatctcta cgctggaaac tttctgcctc aatatttact gtgcctttgt ttttgctagt
     5941 ttgtgttgtt gaaaaaaaaa acattctctg cctgagtttt aatttttgtc caaagttatt
     6001 ttaatctata caattaaaag cttttgccta tcactct
//
