LOCUS       NM_001405686            6316 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 21, mRNA.
ACCESSION   NM_001405686
VERSION     NM_001405686.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6316)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6316)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6316)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6316)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6316)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6316)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6316)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6316)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405686.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-578             AC277909.1         203688-203766
            579-648             AC277909.1         204036-204105
            649-961             AC277909.1         204942-205254
            962-1116            AC277909.1         206382-206536
            1117-1326           AC277909.1         206639-206848
            1327-1429           AC277909.1         208694-208796
            1430-1644           AC277909.1         209632-209846
            1645-1717           AC277909.1         210898-210970
            1718-1929           AC277909.1         211434-211645
            1930-2139           AC277909.1         212992-213201
            2140-2242           AC254635.1         129707-129809
            2243-2457           AC254635.1         130645-130859
            2458-2530           AC254635.1         131910-131982
            2531-2742           AC254635.1         132446-132657
            2743-2952           AC254635.1         134007-134216
            2953-3055           AC254635.1         135990-136092
            3056-3270           AC254635.1         136928-137142
            3271-3343           AC254635.1         138180-138252
            3344-3549           AC254635.1         138717-138922
            3550-3601           AC254635.1         140214-140265
            3602-3765           AC254635.1         144172-144335
            3766-3817           AC254635.1         145378-145429
            3818-3990           AC254635.1         146069-146241
            3991-4042           AC254635.1         146961-147012
            4043-4215           AC254635.1         147615-147787
            4216-4267           AC254635.1         148497-148548
            4268-4440           AC254635.1         149171-149343
            4441-4552           AC254635.1         150058-150169
            4553-6316           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6316
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..578
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            579..648
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            649..961
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1116
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1326
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             1152..4796
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 7 is encoded by transcript variant 21;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 7"
                     /protein_id="NP_001392615.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPC
                     DSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLREKLREGRDASR
                     SLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEA
                     EKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVN
                     STLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKL
                     HPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKL
                     AEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDEPDKSQ
                     GQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAPREMPKAEEKE
                     VPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHII
                     PENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYQSYSGTF
                     HSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTP
                     SGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDE
                     KEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEE
                     EDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKH
                     VGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSGVLMEVEEPE
                     VLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVDNRFLTLMGTSLHL
                     VFQMGVIFPQ"
     exon            1327..1429
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1644
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1717
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1929
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2139
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2242
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2457
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2458..2530
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2742
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2952
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2953..3055
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3270
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3343
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3549
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3550..3601
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3602..3765
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3766..3817
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3818..3990
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3991..4042
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4043..4215
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4216..4267
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4268..4440
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4441..4552
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4553..6316
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6292..6297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      6312
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacaga tggccaatct ttcctaagct gctcacctta caagaaaacg
      541 aatcgtactg ctaagaattc aaacttcagc agtcatgggg agccttggaa ggagcccgaa
      601 tcactgatgg aattggacag tgcatggaga tggttcagca ggacaaggtg ctgaaaactc
      661 atccccgtgt gacctcaagt aagccatgta actctgtgaa cctgcagttt tatcattttt
      721 aaaataaaga aacatgacag attttcatta tgacacagaa tgtcaggtct cccagatgcc
      781 agaaaataca tttacttaaa gccgttgata cgtcttaaag cggtttcctt acagtgtcat
      841 tggaggacag tgtggagtgc agagagacat gctttgaaat gggattgatc cagtcctcct
      901 tccttcacta ccacatgaat gctgggcagc ccagggtcaa cccaccgcac cctcaactca
      961 gacggttacc tggcacgctg gccacagtct acctcactct tatcagagtc tgagctactg
     1021 gcagtgcttt cagctctgag ttcaggcacc tcgaaccttg tttttgtggt gaaggatcct
     1081 aaagtgctgt ggggagtgat cacatttttc acaacatccc tggctccacc tcttctgcca
     1141 caaacgtcag catggtggta tcagctggcc cttggtccag cgagaaggca gagacgaaca
     1201 ttttagaaat caacgagaaa ttgcgccccc agctggcaga gaagaaacag cagttcagaa
     1261 acctcaaaga gaaatgtttt gtaactcaac tggccggctt cctggccaac cgacagaaga
     1321 aatacaagta tgaagagtgt aaagacctca taaaatttat gctgaggaat gagcgacagt
     1381 tcaaggagga gaagcttgca gagcagctca agcaagctga ggagctcagg caatataaag
     1441 tcctggttca ctctcaggaa cgggagctga cccagttaag ggagaagtta cgggaaggga
     1501 gagatgcctc ccgctcattg aatcagcatc tccaggccct cctcactccg gataagccag
     1561 acaagtccca ggggcaggac ctccaagaac agctggctga ggggtgtaga ctggcacagc
     1621 aacttttcca aaagctcagc ccagaaaatg acgaagatga ggatgaagat gttcaagttg
     1681 aggaggctga gaaagtactg gaatcatctg cccccaggga ggtgcagaag gctgaagaaa
     1741 gcaaagtccc tgaggactca ctggaggaat gtgccatcac ttgttcaaat agccacggcc
     1801 cttgtgactc caaccagcct cacaagaaca tcaacatcac ctttgaggaa gacaaagtca
     1861 actcagctct ggttgtagac agagaatcct ctcatgatga atgtcaggat gctgtaaaca
     1921 ttctcccagt ccctggcccc acctcttctg ccacaaacgt cagcatggtg gtatcagccg
     1981 gccctttgtt cagcgagaag gcagagatga acattctaga aatcaacgag aaattgcatc
     2041 cccagctggc agagaagaat cagcagttca gaaacctcaa agagaaatgt tttgtaactc
     2101 aactggcctg cttcctggcc aaccagcaga acaaatacaa atatgaagag tgcaaagacc
     2161 tcataaaatc tatgctgagg aaagagcgac agttcaagga ggagaagctt gcagagcagc
     2221 tcaagcaagc tgaggagctc aggcaatata aagtcctggt tcactctcag gaacgagagc
     2281 tgacccactt aagggagaag ttacgggaag ggagagatgc ctcccgctca ttgaatcagc
     2341 atctccaggc cctcctcact ccggataagc cagacaagtc ccaggggcag gacctccaag
     2401 aacagctggc tgaggggtgt agactggcac agcaactttt ccagaagctc agcccagaaa
     2461 atgacgaaga tgaggatgaa gatgttcaag ttgaggaggc tgagaaagta ctggaatcat
     2521 ctgcccccag ggaggtgcag aaggctgaag aaagcaaagt ccctgaggac tcactggagg
     2581 aatgtgccat cacttgttca aatagccaca gcccttgtga ctccaaccag cctcacaaga
     2641 acatcaacat cacctttgag gaagacaaag tcaactcaac tctggttgta gacagagaat
     2701 cctctcatga tgaatgtcag gatgctgtaa acattctccc agtccctggc cccacctctt
     2761 ctgccacaaa cgtcagcatg gtggtatcag ccggcccctt gtccagcgag aaggcagaga
     2821 tgaacattct agaaatcaac gagaaattgc atccccagct ggcagagaag aaacagcagt
     2881 tcagaaacct caaagagaaa tgttttgtaa ctcaactggc ctgcttcctg gccaaccagc
     2941 agaacaaata caaatatgaa gagtgcaaag acctcataaa atctatgctg aggaatgagc
     3001 gacagttcaa ggaggagaag cttgcagagc agctcaagca agctgaggag ctcaggcaat
     3061 ataaagtcct ggttcactct caggaacggg agctgaccca gttaagggag aagttacggg
     3121 aagggagaga tgcctcccgc tcattgaatc agcatctcca ggccctcctc actccggatg
     3181 agccagacaa gtcccagggg caggacctcc aagaacagct ggctgagggg tgtagactgg
     3241 cacagcacct tgtccaaaag ctcagcccag aaaatgacaa tgatgacgat gaagatgttc
     3301 aagttgaggt ggctgagaaa gtgcaggaat cgtctgcccc cagggagatg ccgaaggctg
     3361 aagaaaagga agtccctgag gactcactgg aggaatgtgc catcacttgt tcaaatagcc
     3421 atggccctta tgactccaac cagccacata ggaaaaccaa aatcacattt gaggaagaca
     3481 aagtcgactc aactctcatt ggctcatcct ctcatgttga atgggaggat gctgtacaca
     3541 ttatcccaga aaatgaaagt gatgatgagg aagaggaaga aaaagggcca gtgtctccca
     3601 ggaatctgca ggagtctgaa gaggaggaag tcccccagga gtcctgggat gaaggttatt
     3661 cgactctctc aattcctcct gaaatgttgg cctcgtacca gtcttacagc ggcacatttc
     3721 actcattaga ggaacagcaa gtctgcatgg ctgttgacat aggcggacat cggtgggatc
     3781 aagtgaaaaa ggaggaccaa gaggcaacag gtcccaggct cagcagggag ctgctggatg
     3841 agaaagggcc tgaagtcttg caggactcac tggatagatg ttattcaact ccttcaggtt
     3901 atcttgaact gactgactca tgccagccct acagaagtgc cttttacata ttggagcaac
     3961 agcgtgttgg ctgggctctt gacatggatg aaattgaaaa gtaccaagaa gtggaagaag
     4021 accaagaccc atcatgcccc aggctcagca gggagctgct ggatgagaaa gagcctgaag
     4081 tcttgcagga ctcactggat agatgttatt cgactccttc aggttatctt gaactgcctg
     4141 acttaggcca gccctacaga agtgctgttt actcattgga ggaacagtac cttggcttgg
     4201 ctcttgacgt ggacagaatt aaaaaggacc aggaagagga agaagaccaa ggcccaccat
     4261 gccccaggct cagcagggag ctgctggagg cagtagagcc tgaagtcttg caggactcac
     4321 tggatagatg ttattcaact ccttccagtt gtcttgaaca gcctgactcc tgcctgccct
     4381 atggaagttc cttttatgca ttggaggaaa aacatgttgg cttttctctt gacgtgggag
     4441 aaattgaaaa gaaggggaag gggaagaaaa gaaggggaag aagatcaacg aagaaaagaa
     4501 ggagaagggg aagaaaagaa ggggaagaag atcaaaaccc accatgcccc aggctcagcg
     4561 gtgtgctgat ggaagtggaa gagcctgaag tcttacagga ctcactggat agatgttatt
     4621 cgactccgtc aatgttcttt gaactacctg actcattcca gcactacaga agtgtgtttt
     4681 actcatttga ggaacagcac atcagcttcg cccttgacgt ggacaatagg tttcttactt
     4741 tgatgggaac aagtctccac ctggtcttcc agatgggagt catattccca cagtaagcag
     4801 cccttactaa gccgagagat gtcattcctg caggcaggac ctataggcac gtgaagattt
     4861 gaatgaaact atagttccat ttggaagccc agacatagga tgggtcagtg ggcatggctc
     4921 tattcctatt ctcagaccat gccagtggca acctgtgctc agtctgaaga caatggaccc
     4981 aagttaggtg tgacacgttc acataactgt gcagcacatg ccgggagtga tcagtcagac
     5041 attttaattt gaaccacgta tctctgggta gctacaaagt tcctcaggga tttcattttg
     5101 caggcatgtc tctgagcttc tatacctgct caaggtcagt gtcatctttg tgtttagctc
     5161 atccaaaggt gttaccctgg tttcaatgaa cctaacctca ttctttgtat cttcagtgtt
     5221 gaattgtttt agctgatcca tctttaacgc aggagggatc cttggctgag gattgtattt
     5281 cagaaccacc aactgctctt gacaattgtt aacccgctag gctcctttgg ttagagaagc
     5341 cacagtcctt cagcctccaa ttggtgtcag tacttaggaa gaccacagct agatggacaa
     5401 acagcattgg gaggccttag ccctgctcct ctcgattcca tcctgtagag aacaggagtc
     5461 aggagccgct ggcaggagac agcatgtcac ccaggactct gccggtgcag aatatgaaca
     5521 acgccatgtt cttgcagaaa acgcttagcc tgagtttcat aggaggtaat caccagacaa
     5581 ctgcagaatg tagaacactg agcaggacaa ctgacctgtc tccttcacat agtccatatc
     5641 accacaaatc acacaacaaa aaggagaaga gatattttgg gttcaaaaaa agtaaaaaga
     5701 taatatagct gcatttcttt agttattttg aaccccaaat atttcctcat ctttttgttg
     5761 ttgtcattga tggtggtgac atggacttgt ttatagagga caggtcagct gtctggctca
     5821 atgatctaca ttctgaagtt gtctgaaaat gtcttcatga ttaaattcag cctaaacgtt
     5881 ttgccgggaa cactgcagag acaatgctgt gagtttccaa ccttagccca tctgcgggca
     5941 gagaaggtct agtttgtcca tcagcattat catgatatca ggactggtta cttggttaag
     6001 gaggggtcta ggagatctgt cccttttaga gacaccttac ttataatgaa gtatttggga
     6061 gggtggtttt caaaagtaga aatgtcctgt attccgatga tcatcctgta aacattttat
     6121 catttattaa tcatccctgc ctgtgtctat tattatattc atatctctac gctggaaact
     6181 ttctgcctca atatttactg tgcctttgtt tttgctagtt tgtgttgttg aaaaaaaaaa
     6241 cattctctgc ctgagtttta atttttgtcc aaagttattt taatctatac aattaaaagc
     6301 ttttgcctat cactct
//
