LOCUS       NM_001405681            6404 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 16, mRNA.
ACCESSION   NM_001405681
VERSION     NM_001405681.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6404)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6404)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6404)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6404)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6404)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6404)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6404)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6404)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405681.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-582             AC277909.1         203684-203766
            583-652             AC277909.1         204036-204105
            653-965             AC277909.1         204942-205254
            966-1120            AC277909.1         206382-206536
            1121-1330           AC277909.1         206639-206848
            1331-1433           AC277909.1         208694-208796
            1434-1648           AC277909.1         209632-209846
            1649-1721           AC277909.1         210898-210970
            1722-1933           AC277909.1         211434-211645
            1934-2143           AC277909.1         212992-213201
            2144-2246           AC254635.1         129707-129809
            2247-2461           AC254635.1         130645-130859
            2462-2534           AC254635.1         131910-131982
            2535-2746           AC254635.1         132446-132657
            2747-2956           AC254635.1         134007-134216
            2957-3059           AC254635.1         135990-136092
            3060-3274           AC254635.1         136928-137142
            3275-3347           AC254635.1         138180-138252
            3348-3553           AC254635.1         138717-138922
            3554-3605           AC254635.1         140214-140265
            3606-3689           AC254635.1         142237-142320
            3690-3853           AC254635.1         144172-144335
            3854-3905           AC254635.1         145378-145429
            3906-4078           AC254635.1         146069-146241
            4079-4130           AC254635.1         146961-147012
            4131-4303           AC254635.1         147615-147787
            4304-4355           AC254635.1         148497-148548
            4356-4528           AC254635.1         149171-149343
            4529-4640           AC254635.1         150058-150169
            4641-6404           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6404
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..582
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            583..652
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            653..965
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1120
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1330
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             1156..4884
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 6 is encoded by transcript variant 16;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 6"
                     /protein_id="NP_001392610.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPC
                     DSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLREKLREGRDASR
                     SLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEA
                     EKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVN
                     STLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKL
                     HPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKL
                     AEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDEPDKSQ
                     GQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAPREMPKAEEKE
                     VPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHII
                     PENESDDEEEEEKGPVSPRGMDEAGNHHSQQTIARTKSQVPHVLTHRNLQESEEEEVP
                     QESWDEGYSTLSIPPEMLASYQSYSGTFHSLEEQQVCMAVDIGGHRWDQVKKEDQEAT
                     GPRLSRELLDEKGPEVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALD
                     MDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPY
                     RSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRC
                     YSTPSSCLEQPDSCLPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRR
                     RGRKEGEEDQNPPCPRLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVF
                     YSFEEQHISFALDVDNRFLTLMGTSLHLVFQMGVIFPQ"
     exon            1331..1433
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1648
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1721
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1933
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2143
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2246
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2461
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2534
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2746
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2956
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3059
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3060..3274
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3347
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3348..3553
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3554..3605
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3606..3689
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3690..3853
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3854..3905
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3906..4078
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..4130
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4131..4303
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4304..4355
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4528
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4529..4640
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4641..6404
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6380..6385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      6400
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacagg aagatggcca atctttccta agctgctcac cttacaagaa
      541 aacgaatcgt actgctaaga attcaaactt cagcagtcat ggggagcctt ggaaggagcc
      601 cgaatcactg atggaattgg acagtgcatg gagatggttc agcaggacaa ggtgctgaaa
      661 actcatcccc gtgtgacctc aagtaagcca tgtaactctg tgaacctgca gttttatcat
      721 ttttaaaata aagaaacatg acagattttc attatgacac agaatgtcag gtctcccaga
      781 tgccagaaaa tacatttact taaagccgtt gatacgtctt aaagcggttt ccttacagtg
      841 tcattggagg acagtgtgga gtgcagagag acatgctttg aaatgggatt gatccagtcc
      901 tccttccttc actaccacat gaatgctggg cagcccaggg tcaacccacc gcaccctcaa
      961 ctcagacggt tacctggcac gctggccaca gtctacctca ctcttatcag agtctgagct
     1021 actggcagtg ctttcagctc tgagttcagg cacctcgaac cttgtttttg tggtgaagga
     1081 tcctaaagtg ctgtggggag tgatcacatt tttcacaaca tccctggctc cacctcttct
     1141 gccacaaacg tcagcatggt ggtatcagct ggcccttggt ccagcgagaa ggcagagacg
     1201 aacattttag aaatcaacga gaaattgcgc ccccagctgg cagagaagaa acagcagttc
     1261 agaaacctca aagagaaatg ttttgtaact caactggccg gcttcctggc caaccgacag
     1321 aagaaataca agtatgaaga gtgtaaagac ctcataaaat ttatgctgag gaatgagcga
     1381 cagttcaagg aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat
     1441 aaagtcctgg ttcactctca ggaacgggag ctgacccagt taagggagaa gttacgggaa
     1501 gggagagatg cctcccgctc attgaatcag catctccagg ccctcctcac tccggataag
     1561 ccagacaagt cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactggca
     1621 cagcaacttt tccaaaagct cagcccagaa aatgacgaag atgaggatga agatgttcaa
     1681 gttgaggagg ctgagaaagt actggaatca tctgccccca gggaggtgca gaaggctgaa
     1741 gaaagcaaag tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccac
     1801 ggcccttgtg actccaacca gcctcacaag aacatcaaca tcacctttga ggaagacaaa
     1861 gtcaactcag ctctggttgt agacagagaa tcctctcatg atgaatgtca ggatgctgta
     1921 aacattctcc cagtccctgg ccccacctct tctgccacaa acgtcagcat ggtggtatca
     1981 gccggccctt tgttcagcga gaaggcagag atgaacattc tagaaatcaa cgagaaattg
     2041 catccccagc tggcagagaa gaatcagcag ttcagaaacc tcaaagagaa atgttttgta
     2101 actcaactgg cctgcttcct ggccaaccag cagaacaaat acaaatatga agagtgcaaa
     2161 gacctcataa aatctatgct gaggaaagag cgacagttca aggaggagaa gcttgcagag
     2221 cagctcaagc aagctgagga gctcaggcaa tataaagtcc tggttcactc tcaggaacga
     2281 gagctgaccc acttaaggga gaagttacgg gaagggagag atgcctcccg ctcattgaat
     2341 cagcatctcc aggccctcct cactccggat aagccagaca agtcccaggg gcaggacctc
     2401 caagaacagc tggctgaggg gtgtagactg gcacagcaac ttttccagaa gctcagccca
     2461 gaaaatgacg aagatgagga tgaagatgtt caagttgagg aggctgagaa agtactggaa
     2521 tcatctgccc ccagggaggt gcagaaggct gaagaaagca aagtccctga ggactcactg
     2581 gaggaatgtg ccatcacttg ttcaaatagc cacagccctt gtgactccaa ccagcctcac
     2641 aagaacatca acatcacctt tgaggaagac aaagtcaact caactctggt tgtagacaga
     2701 gaatcctctc atgatgaatg tcaggatgct gtaaacattc tcccagtccc tggccccacc
     2761 tcttctgcca caaacgtcag catggtggta tcagccggcc ccttgtccag cgagaaggca
     2821 gagatgaaca ttctagaaat caacgagaaa ttgcatcccc agctggcaga gaagaaacag
     2881 cagttcagaa acctcaaaga gaaatgtttt gtaactcaac tggcctgctt cctggccaac
     2941 cagcagaaca aatacaaata tgaagagtgc aaagacctca taaaatctat gctgaggaat
     3001 gagcgacagt tcaaggagga gaagcttgca gagcagctca agcaagctga ggagctcagg
     3061 caatataaag tcctggttca ctctcaggaa cgggagctga cccagttaag ggagaagtta
     3121 cgggaaggga gagatgcctc ccgctcattg aatcagcatc tccaggccct cctcactccg
     3181 gatgagccag acaagtccca ggggcaggac ctccaagaac agctggctga ggggtgtaga
     3241 ctggcacagc accttgtcca aaagctcagc ccagaaaatg acaatgatga cgatgaagat
     3301 gttcaagttg aggtggctga gaaagtgcag gaatcgtctg cccccaggga gatgccgaag
     3361 gctgaagaaa aggaagtccc tgaggactca ctggaggaat gtgccatcac ttgttcaaat
     3421 agccatggcc cttatgactc caaccagcca cataggaaaa ccaaaatcac atttgaggaa
     3481 gacaaagtcg actcaactct cattggctca tcctctcatg ttgaatggga ggatgctgta
     3541 cacattatcc cagaaaatga aagtgatgat gaggaagagg aagaaaaagg gccagtgtct
     3601 cccaggggca tggatgaagc tggaaaccat cattctcagc aaactatcgc aaggacaaaa
     3661 agccaagtac cgcatgttct tactcacagg aatctgcagg agtctgaaga ggaggaagtc
     3721 ccccaggagt cctgggatga aggttattcg actctctcaa ttcctcctga aatgttggcc
     3781 tcgtaccagt cttacagcgg cacatttcac tcattagagg aacagcaagt ctgcatggct
     3841 gttgacatag gcggacatcg gtgggatcaa gtgaaaaagg aggaccaaga ggcaacaggt
     3901 cccaggctca gcagggagct gctggatgag aaagggcctg aagtcttgca ggactcactg
     3961 gatagatgtt attcaactcc ttcaggttat cttgaactga ctgactcatg ccagccctac
     4021 agaagtgcct tttacatatt ggagcaacag cgtgttggct gggctcttga catggatgaa
     4081 attgaaaagt accaagaagt ggaagaagac caagacccat catgccccag gctcagcagg
     4141 gagctgctgg atgagaaaga gcctgaagtc ttgcaggact cactggatag atgttattcg
     4201 actccttcag gttatcttga actgcctgac ttaggccagc cctacagaag tgctgtttac
     4261 tcattggagg aacagtacct tggcttggct cttgacgtgg acagaattaa aaaggaccag
     4321 gaagaggaag aagaccaagg cccaccatgc cccaggctca gcagggagct gctggaggca
     4381 gtagagcctg aagtcttgca ggactcactg gatagatgtt attcaactcc ttccagttgt
     4441 cttgaacagc ctgactcctg cctgccctat ggaagttcct tttatgcatt ggaggaaaaa
     4501 catgttggct tttctcttga cgtgggagaa attgaaaaga aggggaaggg gaagaaaaga
     4561 aggggaagaa gatcaacgaa gaaaagaagg agaaggggaa gaaaagaagg ggaagaagat
     4621 caaaacccac catgccccag gctcagcggt gtgctgatgg aagtggaaga gcctgaagtc
     4681 ttacaggact cactggatag atgttattcg actccgtcaa tgttctttga actacctgac
     4741 tcattccagc actacagaag tgtgttttac tcatttgagg aacagcacat cagcttcgcc
     4801 cttgacgtgg acaataggtt tcttactttg atgggaacaa gtctccacct ggtcttccag
     4861 atgggagtca tattcccaca gtaagcagcc cttactaagc cgagagatgt cattcctgca
     4921 ggcaggacct ataggcacgt gaagatttga atgaaactat agttccattt ggaagcccag
     4981 acataggatg ggtcagtggg catggctcta ttcctattct cagaccatgc cagtggcaac
     5041 ctgtgctcag tctgaagaca atggacccaa gttaggtgtg acacgttcac ataactgtgc
     5101 agcacatgcc gggagtgatc agtcagacat tttaatttga accacgtatc tctgggtagc
     5161 tacaaagttc ctcagggatt tcattttgca ggcatgtctc tgagcttcta tacctgctca
     5221 aggtcagtgt catctttgtg tttagctcat ccaaaggtgt taccctggtt tcaatgaacc
     5281 taacctcatt ctttgtatct tcagtgttga attgttttag ctgatccatc tttaacgcag
     5341 gagggatcct tggctgagga ttgtatttca gaaccaccaa ctgctcttga caattgttaa
     5401 cccgctaggc tcctttggtt agagaagcca cagtccttca gcctccaatt ggtgtcagta
     5461 cttaggaaga ccacagctag atggacaaac agcattggga ggccttagcc ctgctcctct
     5521 cgattccatc ctgtagagaa caggagtcag gagccgctgg caggagacag catgtcaccc
     5581 aggactctgc cggtgcagaa tatgaacaac gccatgttct tgcagaaaac gcttagcctg
     5641 agtttcatag gaggtaatca ccagacaact gcagaatgta gaacactgag caggacaact
     5701 gacctgtctc cttcacatag tccatatcac cacaaatcac acaacaaaaa ggagaagaga
     5761 tattttgggt tcaaaaaaag taaaaagata atatagctgc atttctttag ttattttgaa
     5821 ccccaaatat ttcctcatct ttttgttgtt gtcattgatg gtggtgacat ggacttgttt
     5881 atagaggaca ggtcagctgt ctggctcaat gatctacatt ctgaagttgt ctgaaaatgt
     5941 cttcatgatt aaattcagcc taaacgtttt gccgggaaca ctgcagagac aatgctgtga
     6001 gtttccaacc ttagcccatc tgcgggcaga gaaggtctag tttgtccatc agcattatca
     6061 tgatatcagg actggttact tggttaagga ggggtctagg agatctgtcc cttttagaga
     6121 caccttactt ataatgaagt atttgggagg gtggttttca aaagtagaaa tgtcctgtat
     6181 tccgatgatc atcctgtaaa cattttatca tttattaatc atccctgcct gtgtctatta
     6241 ttatattcat atctctacgc tggaaacttt ctgcctcaat atttactgtg cctttgtttt
     6301 tgctagtttg tgttgttgaa aaaaaaaaca ttctctgcct gagttttaat ttttgtccaa
     6361 agttatttta atctatacaa ttaaaagctt ttgcctatca ctct
//
