LOCUS       NM_001405680            6218 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 15, mRNA.
ACCESSION   NM_001405680
VERSION     NM_001405680.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6218)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6218)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6218)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6218)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6218)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6218)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6218)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6218)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405680.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-582             AC277909.1         203684-203766
            583-652             AC277909.1         204036-204105
            653-779             AC277909.1         205128-205254
            780-934             AC277909.1         206382-206536
            935-1144            AC277909.1         206639-206848
            1145-1247           AC277909.1         208694-208796
            1248-1462           AC277909.1         209632-209846
            1463-1535           AC277909.1         210898-210970
            1536-1747           AC277909.1         211434-211645
            1748-1957           AC277909.1         212992-213201
            1958-2060           AC254635.1         129707-129809
            2061-2275           AC254635.1         130645-130859
            2276-2348           AC254635.1         131910-131982
            2349-2560           AC254635.1         132446-132657
            2561-2770           AC254635.1         134007-134216
            2771-2873           AC254635.1         135990-136092
            2874-3088           AC254635.1         136928-137142
            3089-3161           AC254635.1         138180-138252
            3162-3367           AC254635.1         138717-138922
            3368-3419           AC254635.1         140214-140265
            3420-3503           AC254635.1         142237-142320
            3504-3667           AC254635.1         144172-144335
            3668-3719           AC254635.1         145378-145429
            3720-3892           AC254635.1         146069-146241
            3893-3944           AC254635.1         146961-147012
            3945-4117           AC254635.1         147615-147787
            4118-4169           AC254635.1         148497-148548
            4170-4342           AC254635.1         149171-149343
            4343-4454           AC254635.1         150058-150169
            4455-6218           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6218
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..582
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            583..652
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            653..779
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            780..934
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1144
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             970..4698
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 6 is encoded by transcript variant 15;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 6"
                     /protein_id="NP_001392609.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPC
                     DSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLREKLREGRDASR
                     SLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEA
                     EKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVN
                     STLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKL
                     HPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKL
                     AEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDEPDKSQ
                     GQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAPREMPKAEEKE
                     VPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHII
                     PENESDDEEEEEKGPVSPRGMDEAGNHHSQQTIARTKSQVPHVLTHRNLQESEEEEVP
                     QESWDEGYSTLSIPPEMLASYQSYSGTFHSLEEQQVCMAVDIGGHRWDQVKKEDQEAT
                     GPRLSRELLDEKGPEVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALD
                     MDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPY
                     RSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRC
                     YSTPSSCLEQPDSCLPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRR
                     RGRKEGEEDQNPPCPRLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVF
                     YSFEEQHISFALDVDNRFLTLMGTSLHLVFQMGVIFPQ"
     exon            1145..1247
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1462
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1535
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1747
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1957
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2060
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2275
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2348
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2349..2560
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2770
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2771..2873
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2874..3088
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3089..3161
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3162..3367
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3368..3419
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3503
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..3667
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3668..3719
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3720..3892
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3893..3944
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3945..4117
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4118..4169
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4170..4342
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4343..4454
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4455..6218
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6194..6199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      6214
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacagg aagatggcca atctttccta agctgctcac cttacaagaa
      541 aacgaatcgt actgctaaga attcaaactt cagcagtcat ggggagcctt ggaaggagcc
      601 cgaatcactg atggaattgg acagtgcatg gagatggttc agcaggacaa ggtgtcattg
      661 gaggacagtg tggagtgcag agagacatgc tttgaaatgg gattgatcca gtcctccttc
      721 cttcactacc acatgaatgc tgggcagccc agggtcaacc caccgcaccc tcaactcaga
      781 cggttacctg gcacgctggc cacagtctac ctcactctta tcagagtctg agctactggc
      841 agtgctttca gctctgagtt caggcacctc gaaccttgtt tttgtggtga aggatcctaa
      901 agtgctgtgg ggagtgatca catttttcac aacatccctg gctccacctc ttctgccaca
      961 aacgtcagca tggtggtatc agctggccct tggtccagcg agaaggcaga gacgaacatt
     1021 ttagaaatca acgagaaatt gcgcccccag ctggcagaga agaaacagca gttcagaaac
     1081 ctcaaagaga aatgttttgt aactcaactg gccggcttcc tggccaaccg acagaagaaa
     1141 tacaagtatg aagagtgtaa agacctcata aaatttatgc tgaggaatga gcgacagttc
     1201 aaggaggaga agcttgcaga gcagctcaag caagctgagg agctcaggca atataaagtc
     1261 ctggttcact ctcaggaacg ggagctgacc cagttaaggg agaagttacg ggaagggaga
     1321 gatgcctccc gctcattgaa tcagcatctc caggccctcc tcactccgga taagccagac
     1381 aagtcccagg ggcaggacct ccaagaacag ctggctgagg ggtgtagact ggcacagcaa
     1441 cttttccaaa agctcagccc agaaaatgac gaagatgagg atgaagatgt tcaagttgag
     1501 gaggctgaga aagtactgga atcatctgcc cccagggagg tgcagaaggc tgaagaaagc
     1561 aaagtccctg aggactcact ggaggaatgt gccatcactt gttcaaatag ccacggccct
     1621 tgtgactcca accagcctca caagaacatc aacatcacct ttgaggaaga caaagtcaac
     1681 tcagctctgg ttgtagacag agaatcctct catgatgaat gtcaggatgc tgtaaacatt
     1741 ctcccagtcc ctggccccac ctcttctgcc acaaacgtca gcatggtggt atcagccggc
     1801 cctttgttca gcgagaaggc agagatgaac attctagaaa tcaacgagaa attgcatccc
     1861 cagctggcag agaagaatca gcagttcaga aacctcaaag agaaatgttt tgtaactcaa
     1921 ctggcctgct tcctggccaa ccagcagaac aaatacaaat atgaagagtg caaagacctc
     1981 ataaaatcta tgctgaggaa agagcgacag ttcaaggagg agaagcttgc agagcagctc
     2041 aagcaagctg aggagctcag gcaatataaa gtcctggttc actctcagga acgagagctg
     2101 acccacttaa gggagaagtt acgggaaggg agagatgcct cccgctcatt gaatcagcat
     2161 ctccaggccc tcctcactcc ggataagcca gacaagtccc aggggcagga cctccaagaa
     2221 cagctggctg aggggtgtag actggcacag caacttttcc agaagctcag cccagaaaat
     2281 gacgaagatg aggatgaaga tgttcaagtt gaggaggctg agaaagtact ggaatcatct
     2341 gcccccaggg aggtgcagaa ggctgaagaa agcaaagtcc ctgaggactc actggaggaa
     2401 tgtgccatca cttgttcaaa tagccacagc ccttgtgact ccaaccagcc tcacaagaac
     2461 atcaacatca cctttgagga agacaaagtc aactcaactc tggttgtaga cagagaatcc
     2521 tctcatgatg aatgtcagga tgctgtaaac attctcccag tccctggccc cacctcttct
     2581 gccacaaacg tcagcatggt ggtatcagcc ggccccttgt ccagcgagaa ggcagagatg
     2641 aacattctag aaatcaacga gaaattgcat ccccagctgg cagagaagaa acagcagttc
     2701 agaaacctca aagagaaatg ttttgtaact caactggcct gcttcctggc caaccagcag
     2761 aacaaataca aatatgaaga gtgcaaagac ctcataaaat ctatgctgag gaatgagcga
     2821 cagttcaagg aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat
     2881 aaagtcctgg ttcactctca ggaacgggag ctgacccagt taagggagaa gttacgggaa
     2941 gggagagatg cctcccgctc attgaatcag catctccagg ccctcctcac tccggatgag
     3001 ccagacaagt cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactggca
     3061 cagcaccttg tccaaaagct cagcccagaa aatgacaatg atgacgatga agatgttcaa
     3121 gttgaggtgg ctgagaaagt gcaggaatcg tctgccccca gggagatgcc gaaggctgaa
     3181 gaaaaggaag tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccat
     3241 ggcccttatg actccaacca gccacatagg aaaaccaaaa tcacatttga ggaagacaaa
     3301 gtcgactcaa ctctcattgg ctcatcctct catgttgaat gggaggatgc tgtacacatt
     3361 atcccagaaa atgaaagtga tgatgaggaa gaggaagaaa aagggccagt gtctcccagg
     3421 ggcatggatg aagctggaaa ccatcattct cagcaaacta tcgcaaggac aaaaagccaa
     3481 gtaccgcatg ttcttactca caggaatctg caggagtctg aagaggagga agtcccccag
     3541 gagtcctggg atgaaggtta ttcgactctc tcaattcctc ctgaaatgtt ggcctcgtac
     3601 cagtcttaca gcggcacatt tcactcatta gaggaacagc aagtctgcat ggctgttgac
     3661 ataggcggac atcggtggga tcaagtgaaa aaggaggacc aagaggcaac aggtcccagg
     3721 ctcagcaggg agctgctgga tgagaaaggg cctgaagtct tgcaggactc actggataga
     3781 tgttattcaa ctccttcagg ttatcttgaa ctgactgact catgccagcc ctacagaagt
     3841 gccttttaca tattggagca acagcgtgtt ggctgggctc ttgacatgga tgaaattgaa
     3901 aagtaccaag aagtggaaga agaccaagac ccatcatgcc ccaggctcag cagggagctg
     3961 ctggatgaga aagagcctga agtcttgcag gactcactgg atagatgtta ttcgactcct
     4021 tcaggttatc ttgaactgcc tgacttaggc cagccctaca gaagtgctgt ttactcattg
     4081 gaggaacagt accttggctt ggctcttgac gtggacagaa ttaaaaagga ccaggaagag
     4141 gaagaagacc aaggcccacc atgccccagg ctcagcaggg agctgctgga ggcagtagag
     4201 cctgaagtct tgcaggactc actggataga tgttattcaa ctccttccag ttgtcttgaa
     4261 cagcctgact cctgcctgcc ctatggaagt tccttttatg cattggagga aaaacatgtt
     4321 ggcttttctc ttgacgtggg agaaattgaa aagaagggga aggggaagaa aagaagggga
     4381 agaagatcaa cgaagaaaag aaggagaagg ggaagaaaag aaggggaaga agatcaaaac
     4441 ccaccatgcc ccaggctcag cggtgtgctg atggaagtgg aagagcctga agtcttacag
     4501 gactcactgg atagatgtta ttcgactccg tcaatgttct ttgaactacc tgactcattc
     4561 cagcactaca gaagtgtgtt ttactcattt gaggaacagc acatcagctt cgcccttgac
     4621 gtggacaata ggtttcttac tttgatggga acaagtctcc acctggtctt ccagatggga
     4681 gtcatattcc cacagtaagc agcccttact aagccgagag atgtcattcc tgcaggcagg
     4741 acctataggc acgtgaagat ttgaatgaaa ctatagttcc atttggaagc ccagacatag
     4801 gatgggtcag tgggcatggc tctattccta ttctcagacc atgccagtgg caacctgtgc
     4861 tcagtctgaa gacaatggac ccaagttagg tgtgacacgt tcacataact gtgcagcaca
     4921 tgccgggagt gatcagtcag acattttaat ttgaaccacg tatctctggg tagctacaaa
     4981 gttcctcagg gatttcattt tgcaggcatg tctctgagct tctatacctg ctcaaggtca
     5041 gtgtcatctt tgtgtttagc tcatccaaag gtgttaccct ggtttcaatg aacctaacct
     5101 cattctttgt atcttcagtg ttgaattgtt ttagctgatc catctttaac gcaggaggga
     5161 tccttggctg aggattgtat ttcagaacca ccaactgctc ttgacaattg ttaacccgct
     5221 aggctccttt ggttagagaa gccacagtcc ttcagcctcc aattggtgtc agtacttagg
     5281 aagaccacag ctagatggac aaacagcatt gggaggcctt agccctgctc ctctcgattc
     5341 catcctgtag agaacaggag tcaggagccg ctggcaggag acagcatgtc acccaggact
     5401 ctgccggtgc agaatatgaa caacgccatg ttcttgcaga aaacgcttag cctgagtttc
     5461 ataggaggta atcaccagac aactgcagaa tgtagaacac tgagcaggac aactgacctg
     5521 tctccttcac atagtccata tcaccacaaa tcacacaaca aaaaggagaa gagatatttt
     5581 gggttcaaaa aaagtaaaaa gataatatag ctgcatttct ttagttattt tgaaccccaa
     5641 atatttcctc atctttttgt tgttgtcatt gatggtggtg acatggactt gtttatagag
     5701 gacaggtcag ctgtctggct caatgatcta cattctgaag ttgtctgaaa atgtcttcat
     5761 gattaaattc agcctaaacg ttttgccggg aacactgcag agacaatgct gtgagtttcc
     5821 aaccttagcc catctgcggg cagagaaggt ctagtttgtc catcagcatt atcatgatat
     5881 caggactggt tacttggtta aggaggggtc taggagatct gtccctttta gagacacctt
     5941 acttataatg aagtatttgg gagggtggtt ttcaaaagta gaaatgtcct gtattccgat
     6001 gatcatcctg taaacatttt atcatttatt aatcatccct gcctgtgtct attattatat
     6061 tcatatctct acgctggaaa ctttctgcct caatatttac tgtgcctttg tttttgctag
     6121 tttgtgttgt tgaaaaaaaa aacattctct gcctgagttt taatttttgt ccaaagttat
     6181 tttaatctat acaattaaaa gcttttgcct atcactct
//
