LOCUS       NM_001405679            6884 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 14, mRNA.
ACCESSION   NM_001405679
VERSION     NM_001405679.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6884)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6884)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6884)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6884)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6884)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6884)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6884)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6884)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405679.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-582             AC277909.1         203684-203766
            583-652             AC277909.1         204036-204105
            653-965             AC277909.1         204942-205254
            966-1120            AC277909.1         206382-206536
            1121-1330           AC277909.1         206639-206848
            1331-1433           AC277909.1         208694-208796
            1434-1648           AC254635.1         130645-130859
            1649-1721           AC254635.1         131910-131982
            1722-1933           AC254635.1         132446-132657
            1934-2143           AC254635.1         134007-134216
            2144-2246           AC254635.1         135990-136092
            2247-2461           AC254635.1         136928-137142
            2462-2534           AC254635.1         138180-138252
            2535-2740           AC254635.1         138717-138922
            2741-2792           AC254635.1         140214-140265
            2793-2956           AC254635.1         144172-144335
            2957-3008           AC254635.1         145378-145429
            3009-3181           AC254635.1         146069-146241
            3182-3233           AC254635.1         146961-147012
            3234-3406           AC254635.1         147615-147787
            3407-3458           AC254635.1         148497-148548
            3459-3631           AC254635.1         149171-149343
            3632-3743           AC254635.1         150058-150169
            3744-3916           AC254635.1         150776-150948
            3917-3968           AC254635.1         151668-151719
            3969-4048           AC254635.1         152322-152401
            4049-4100           AC254635.1         153204-153255
            4101-4273           AC254635.1         153872-154044
            4274-4385           AC254635.1         154759-154870
            4386-4558           AC254635.1         155477-155649
            4559-4610           AC254635.1         156369-156420
            4611-4783           AC254635.1         157023-157195
            4784-4835           AC254635.1         157905-157956
            4836-5008           AC254635.1         158579-158751
            5009-5120           AC254635.1         159466-159577
            5121-6884           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6884
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..582
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            583..652
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            653..965
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1120
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1330
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             1156..5364
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 5 is encoded by transcript variant 14;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 5"
                     /protein_id="NP_001392608.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTHLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPC
                     DSNQPHKNINITFEEDKVNSTLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLSSEKAEMNILEINEKLHPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASR
                     SLNQHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVA
                     EKVQESSAPREMPKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVD
                     STLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYS
                     TLSIPPEMLASYQSYSGTFHSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELL
                     DEKGPEVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQE
                     VEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEE
                     QYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLE
                     QPDSCLPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEED
                     QNPPCPRLSRELLDEKGPEVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVG
                     WALDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGIKKDQEE
                     EEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEK
                     HVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSRELLDEKGP
                     EVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQ
                     DPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGL
                     ALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSC
                     LPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPC
                     PRLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDV
                     DNRFLTLMGTSLHLVFQMGVIFPQ"
     exon            1331..1433
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1648
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1721
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1933
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2143
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2246
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2461
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2534
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2740
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2792
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2956
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3008
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3181
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3182..3233
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3234..3406
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3407..3458
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3631
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3632..3743
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3744..3916
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3917..3968
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3969..4048
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4049..4100
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..4273
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4274..4385
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4386..4558
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4559..4610
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4611..4783
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4784..4835
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4836..5008
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5009..5120
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5121..6884
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6860..6865
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      6880
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacagg aagatggcca atctttccta agctgctcac cttacaagaa
      541 aacgaatcgt actgctaaga attcaaactt cagcagtcat ggggagcctt ggaaggagcc
      601 cgaatcactg atggaattgg acagtgcatg gagatggttc agcaggacaa ggtgctgaaa
      661 actcatcccc gtgtgacctc aagtaagcca tgtaactctg tgaacctgca gttttatcat
      721 ttttaaaata aagaaacatg acagattttc attatgacac agaatgtcag gtctcccaga
      781 tgccagaaaa tacatttact taaagccgtt gatacgtctt aaagcggttt ccttacagtg
      841 tcattggagg acagtgtgga gtgcagagag acatgctttg aaatgggatt gatccagtcc
      901 tccttccttc actaccacat gaatgctggg cagcccaggg tcaacccacc gcaccctcaa
      961 ctcagacggt tacctggcac gctggccaca gtctacctca ctcttatcag agtctgagct
     1021 actggcagtg ctttcagctc tgagttcagg cacctcgaac cttgtttttg tggtgaagga
     1081 tcctaaagtg ctgtggggag tgatcacatt tttcacaaca tccctggctc cacctcttct
     1141 gccacaaacg tcagcatggt ggtatcagct ggcccttggt ccagcgagaa ggcagagacg
     1201 aacattttag aaatcaacga gaaattgcgc ccccagctgg cagagaagaa acagcagttc
     1261 agaaacctca aagagaaatg ttttgtaact caactggccg gcttcctggc caaccgacag
     1321 aagaaataca agtatgaaga gtgtaaagac ctcataaaat ttatgctgag gaatgagcga
     1381 cagttcaagg aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat
     1441 aaagtcctgg ttcactctca ggaacgagag ctgacccact taagggagaa gttacgggaa
     1501 gggagagatg cctcccgctc attgaatcag catctccagg ccctcctcac tccggataag
     1561 ccagacaagt cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactggca
     1621 cagcaacttt tccagaagct cagcccagaa aatgacgaag atgaggatga agatgttcaa
     1681 gttgaggagg ctgagaaagt actggaatca tctgccccca gggaggtgca gaaggctgaa
     1741 gaaagcaaag tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccac
     1801 agcccttgtg actccaacca gcctcacaag aacatcaaca tcacctttga ggaagacaaa
     1861 gtcaactcaa ctctggttgt agacagagaa tcctctcatg atgaatgtca ggatgctgta
     1921 aacattctcc cagtccctgg ccccacctct tctgccacaa acgtcagcat ggtggtatca
     1981 gccggcccct tgtccagcga gaaggcagag atgaacattc tagaaatcaa cgagaaattg
     2041 catccccagc tggcagagaa gaaacagcag ttcagaaacc tcaaagagaa atgttttgta
     2101 actcaactgg cctgcttcct ggccaaccag cagaacaaat acaaatatga agagtgcaaa
     2161 gacctcataa aatctatgct gaggaatgag cgacagttca aggaggagaa gcttgcagag
     2221 cagctcaagc aagctgagga gctcaggcaa tataaagtcc tggttcactc tcaggaacgg
     2281 gagctgaccc agttaaggga gaagttacgg gaagggagag atgcctcccg ctcattgaat
     2341 cagcatctcc aggccctcct cactccggat gagccagaca agtcccaggg gcaggacctc
     2401 caagaacagc tggctgaggg gtgtagactg gcacagcacc ttgtccaaaa gctcagccca
     2461 gaaaatgaca atgatgacga tgaagatgtt caagttgagg tggctgagaa agtgcaggaa
     2521 tcgtctgccc ccagggagat gccgaaggct gaagaaaagg aagtccctga ggactcactg
     2581 gaggaatgtg ccatcacttg ttcaaatagc catggccctt atgactccaa ccagccacat
     2641 aggaaaacca aaatcacatt tgaggaagac aaagtcgact caactctcat tggctcatcc
     2701 tctcatgttg aatgggagga tgctgtacac attatcccag aaaatgaaag tgatgatgag
     2761 gaagaggaag aaaaagggcc agtgtctccc aggaatctgc aggagtctga agaggaggaa
     2821 gtcccccagg agtcctggga tgaaggttat tcgactctct caattcctcc tgaaatgttg
     2881 gcctcgtacc agtcttacag cggcacattt cactcattag aggaacagca agtctgcatg
     2941 gctgttgaca taggcggaca tcggtgggat caagtgaaaa aggaggacca agaggcaaca
     3001 ggtcccaggc tcagcaggga gctgctggat gagaaagggc ctgaagtctt gcaggactca
     3061 ctggatagat gttattcaac tccttcaggt tatcttgaac tgactgactc atgccagccc
     3121 tacagaagtg ccttttacat attggagcaa cagcgtgttg gctgggctct tgacatggat
     3181 gaaattgaaa agtaccaaga agtggaagaa gaccaagacc catcatgccc caggctcagc
     3241 agggagctgc tggatgagaa agagcctgaa gtcttgcagg actcactgga tagatgttat
     3301 tcgactcctt caggttatct tgaactgcct gacttaggcc agccctacag aagtgctgtt
     3361 tactcattgg aggaacagta ccttggcttg gctcttgacg tggacagaat taaaaaggac
     3421 caggaagagg aagaagacca aggcccacca tgccccaggc tcagcaggga gctgctggag
     3481 gcagtagagc ctgaagtctt gcaggactca ctggatagat gttattcaac tccttccagt
     3541 tgtcttgaac agcctgactc ctgcctgccc tatggaagtt ccttttatgc attggaggaa
     3601 aaacatgttg gcttttctct tgacgtggga gaaattgaaa agaaggggaa ggggaagaaa
     3661 agaaggggaa gaagatcaac gaagaaaaga aggagaaggg gaagaaaaga aggggaagaa
     3721 gatcaaaacc caccatgccc caggctcagc agggagctgc tggatgagaa agggcctgaa
     3781 gtcttgcagg actcactgga tagatgttat tcaactcctt caggttatct tgaactgact
     3841 gactcatgcc agccctacag aagtgccttt tacatattgg agcaacagcg tgttggctgg
     3901 gctcttgaca tggatgaaat tgaaaagtac caagaagtgg aagaagacca agacccatca
     3961 tgccccaggc tcagcaggga gctgctggat gagaaagagc ctgaagtctt gcaggactca
     4021 ctggatagat gttattcgac tccttcagga attaaaaagg accaggaaga ggaagaagac
     4081 caaggcccac catgccccag gctcagcagg gagctgctgg aggcagtaga gcctgaagtc
     4141 ttgcaggact cactggatag atgttattca actccttcca gttgtcttga acagcctgac
     4201 tcctgcctgc cctatggaag ttccttttat gcattggagg aaaaacatgt tggcttttct
     4261 cttgacgtgg gagaaattga aaagaagggg aaggggaaga aaagaagggg aagaagatca
     4321 acgaagaaaa gaaggagaag gggaagaaaa gaaggggaag aagatcaaaa cccaccatgc
     4381 cccaggctca gcagggagct gctggatgag aaagggcctg aagtcttgca ggactcactg
     4441 gatagatgtt attcaactcc ttcaggttat cttgaactga ctgactcatg ccagccctac
     4501 agaagtgcct tttacatatt ggagcaacag cgtgttggct gggctcttga catggatgaa
     4561 attgaaaagt accaagaagt ggaagaagac caagacccat catgccccag gctcagcagg
     4621 gagctgctgg atgagaaaga gcctgaagtc ttgcaggact cactggatag atgttattcg
     4681 actccttcag gttatcttga actgcctgac ttaggccagc cctacagaag tgctgtttac
     4741 tcattggagg aacagtacct tggcttggct cttgacgtgg acagaattaa aaaggaccag
     4801 gaagaggaag aagaccaagg cccaccatgc cccaggctca gcagggagct gctggaggca
     4861 gtagagcctg aagtcttgca ggactcactg gatagatgtt attcaactcc ttccagttgt
     4921 cttgaacagc ctgactcctg cctgccctat ggaagttcct tttatgcatt ggaggaaaaa
     4981 catgttggct tttctcttga cgtgggagaa attgaaaaga aggggaaggg gaagaaaaga
     5041 aggggaagaa gatcaacgaa gaaaagaagg agaaggggaa gaaaagaagg ggaagaagat
     5101 caaaacccac catgccccag gctcagcggt gtgctgatgg aagtggaaga gcctgaagtc
     5161 ttacaggact cactggatag atgttattcg actccgtcaa tgttctttga actacctgac
     5221 tcattccagc actacagaag tgtgttttac tcatttgagg aacagcacat cagcttcgcc
     5281 cttgacgtgg acaataggtt tcttactttg atgggaacaa gtctccacct ggtcttccag
     5341 atgggagtca tattcccaca gtaagcagcc cttactaagc cgagagatgt cattcctgca
     5401 ggcaggacct ataggcacgt gaagatttga atgaaactat agttccattt ggaagcccag
     5461 acataggatg ggtcagtggg catggctcta ttcctattct cagaccatgc cagtggcaac
     5521 ctgtgctcag tctgaagaca atggacccaa gttaggtgtg acacgttcac ataactgtgc
     5581 agcacatgcc gggagtgatc agtcagacat tttaatttga accacgtatc tctgggtagc
     5641 tacaaagttc ctcagggatt tcattttgca ggcatgtctc tgagcttcta tacctgctca
     5701 aggtcagtgt catctttgtg tttagctcat ccaaaggtgt taccctggtt tcaatgaacc
     5761 taacctcatt ctttgtatct tcagtgttga attgttttag ctgatccatc tttaacgcag
     5821 gagggatcct tggctgagga ttgtatttca gaaccaccaa ctgctcttga caattgttaa
     5881 cccgctaggc tcctttggtt agagaagcca cagtccttca gcctccaatt ggtgtcagta
     5941 cttaggaaga ccacagctag atggacaaac agcattggga ggccttagcc ctgctcctct
     6001 cgattccatc ctgtagagaa caggagtcag gagccgctgg caggagacag catgtcaccc
     6061 aggactctgc cggtgcagaa tatgaacaac gccatgttct tgcagaaaac gcttagcctg
     6121 agtttcatag gaggtaatca ccagacaact gcagaatgta gaacactgag caggacaact
     6181 gacctgtctc cttcacatag tccatatcac cacaaatcac acaacaaaaa ggagaagaga
     6241 tattttgggt tcaaaaaaag taaaaagata atatagctgc atttctttag ttattttgaa
     6301 ccccaaatat ttcctcatct ttttgttgtt gtcattgatg gtggtgacat ggacttgttt
     6361 atagaggaca ggtcagctgt ctggctcaat gatctacatt ctgaagttgt ctgaaaatgt
     6421 cttcatgatt aaattcagcc taaacgtttt gccgggaaca ctgcagagac aatgctgtga
     6481 gtttccaacc ttagcccatc tgcgggcaga gaaggtctag tttgtccatc agcattatca
     6541 tgatatcagg actggttact tggttaagga ggggtctagg agatctgtcc cttttagaga
     6601 caccttactt ataatgaagt atttgggagg gtggttttca aaagtagaaa tgtcctgtat
     6661 tccgatgatc atcctgtaaa cattttatca tttattaatc atccctgcct gtgtctatta
     6721 ttatattcat atctctacgc tggaaacttt ctgcctcaat atttactgtg cctttgtttt
     6781 tgctagtttg tgttgttgaa aaaaaaaaca ttctctgcct gagttttaat ttttgtccaa
     6841 agttatttta atctatacaa ttaaaagctt ttgcctatca ctct
//
