LOCUS       NM_001405677            7939 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 12, mRNA.
ACCESSION   NM_001405677
VERSION     NM_001405677.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 7939)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 7939)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 7939)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 7939)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7939)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 7939)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 7939)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 7939)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405677.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-1463            AC277909.1         190985-191948
            1464-1546           AC277909.1         203684-203766
            1547-1616           AC277909.1         204036-204105
            1617-1646           AC277909.1         204942-204971
            1647-1849           AC277909.1         205052-205254
            1850-2004           AC277909.1         206382-206536
            2005-2214           AC277909.1         206639-206848
            2215-2317           AC277909.1         208694-208796
            2318-2532           AC277909.1         209632-209846
            2533-2605           AC277909.1         210898-210970
            2606-2817           AC277909.1         211434-211645
            2818-3027           AC277909.1         212992-213201
            3028-3130           AC254635.1         129707-129809
            3131-3345           AC254635.1         130645-130859
            3346-3418           AC254635.1         131910-131982
            3419-3630           AC254635.1         132446-132657
            3631-3840           AC254635.1         134007-134216
            3841-3943           AC254635.1         135990-136092
            3944-4158           AC254635.1         136928-137142
            4159-4231           AC254635.1         138180-138252
            4232-4437           AC254635.1         138717-138922
            4438-4489           AC254635.1         140214-140265
            4490-4653           AC254635.1         144172-144335
            4654-4705           AC254635.1         145378-145429
            4706-4878           AC254635.1         146069-146241
            4879-4930           AC254635.1         146961-147012
            4931-5103           AC254635.1         147615-147787
            5104-5155           AC254635.1         148497-148548
            5156-5328           AC254635.1         149171-149343
            5329-5440           AC254635.1         150058-150169
            5441-5613           AC254635.1         150776-150948
            5614-5665           AC254635.1         151668-151719
            5666-5838           AC254635.1         152322-152494
            5839-5890           AC254635.1         153204-153255
            5891-6063           AC254635.1         153872-154044
            6064-6175           AC254635.1         154759-154870
            6176-7939           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..7939
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..1463
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1546
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1616
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1646
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1849
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..2004
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..2214
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             2040..6419
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 3 is encoded by transcript variant 12;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 3"
                     /protein_id="NP_001392606.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPC
                     DSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLREKLREGRDASR
                     SLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEA
                     EKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVN
                     STLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKL
                     HPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKL
                     AEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDEPDKSQ
                     GQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAPREMPKAEEKE
                     VPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHII
                     PENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYQSYSGTF
                     HSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTP
                     SGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDE
                     KEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEE
                     EDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKH
                     VGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSRELLDEKGPE
                     VLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQD
                     PSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLA
                     LDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCL
                     PYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCP
                     RLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVD
                     NRFLTLMGTSLHLVFQMGVIFPQ"
     exon            2215..2317
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2532
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2605
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2606..2817
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..3027
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3130
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3345
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3418
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..3630
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3631..3840
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3841..3943
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3944..4158
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4231
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4232..4437
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4489
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4490..4653
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4705
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4878
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4879..4930
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4931..5103
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5104..5155
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5156..5328
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5329..5440
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5441..5613
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5614..5665
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5666..5838
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5839..5890
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5891..6063
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            6064..6175
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            6176..7939
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7915..7920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      7935
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacagg tcctgggaga gtaggagcca gtgcaaggag agaagtccat
      541 tgagaaaggc aaatggatga catcagaccc aggggctgag gtccccaact gcagctgggt
      601 agcttctgga gtggacaagg agcaacaggg aagttcgtgg cctggtgttc tgggatccac
      661 tgtctcatct cattcttgtg ggcaccagaa cgtatccaaa gacaagactc agtgtctctg
      721 gcaacagtga gccagagata gaatgtgttt cagaggagga ggaaggggtt gttgtacccc
      781 atggaaacag tatattgttt tacagtagcg tgtctttctc taataactac ttagcgtgtt
      841 cctgttaatg gaaaatattg gtggtgtaag tttccccact gttctcatct tcatgtaaat
      901 ttgttcattt ccttccttcc ttccttcctt ccttccctac ttccctccaa ctctctttct
      961 ctctcttttt attctttccc tccttcccac ccgccctcca tccctccctt ccttcctcct
     1021 tccctccttc cctcccttct ttcctttctt cctttcttct tttcttcttc tttctctctc
     1081 atgctctctc tttttctttc cttttcattc tctctacttt tttgaagaga tcacactgta
     1141 ctgaaaccta cattattttc caaaatctct ggatctgctt ctgtcttgca ggcagagagc
     1201 tcatccagta gcccttagct cttcccagcc ccctcctttg atttgtgggt gccactgcgg
     1261 cagctgctga gtctcagtgg tttctagtct tcaccaagtt ctgcccaccc agatggtttt
     1321 tacctgtcct caccagaaac ctgcactgtc tagattgctg aggctgcttc tccttaaccg
     1381 atctgctatc tgtattccag gggcacccca gggttagagg taaatggcac aggccttgaa
     1441 atctccaact gctctgactc caggaagatg gccaatcttt cctaagctgc tcaccttaca
     1501 agaaaacgaa tcgtactgct aagaattcaa acttcagcag tcatggggag ccttggaagg
     1561 agcccgaatc actgatggaa ttggacagtg catggagatg gttcagcagg acaaggtgct
     1621 gaaaactcat ccccgtgtga cctcaaaatg tcaggtctcc cagatgccag aaaatacatt
     1681 tacttaaagc cgttgatacg tcttaaagcg gtttccttac agtgtcattg gaggacagtg
     1741 tggagtgcag agagacatgc tttgaaatgg gattgatcca gtcctccttc cttcactacc
     1801 acatgaatgc tgggcagccc agggtcaacc caccgcaccc tcaactcaga cggttacctg
     1861 gcacgctggc cacagtctac ctcactctta tcagagtctg agctactggc agtgctttca
     1921 gctctgagtt caggcacctc gaaccttgtt tttgtggtga aggatcctaa agtgctgtgg
     1981 ggagtgatca catttttcac aacatccctg gctccacctc ttctgccaca aacgtcagca
     2041 tggtggtatc agctggccct tggtccagcg agaaggcaga gacgaacatt ttagaaatca
     2101 acgagaaatt gcgcccccag ctggcagaga agaaacagca gttcagaaac ctcaaagaga
     2161 aatgttttgt aactcaactg gccggcttcc tggccaaccg acagaagaaa tacaagtatg
     2221 aagagtgtaa agacctcata aaatttatgc tgaggaatga gcgacagttc aaggaggaga
     2281 agcttgcaga gcagctcaag caagctgagg agctcaggca atataaagtc ctggttcact
     2341 ctcaggaacg ggagctgacc cagttaaggg agaagttacg ggaagggaga gatgcctccc
     2401 gctcattgaa tcagcatctc caggccctcc tcactccgga taagccagac aagtcccagg
     2461 ggcaggacct ccaagaacag ctggctgagg ggtgtagact ggcacagcaa cttttccaaa
     2521 agctcagccc agaaaatgac gaagatgagg atgaagatgt tcaagttgag gaggctgaga
     2581 aagtactgga atcatctgcc cccagggagg tgcagaaggc tgaagaaagc aaagtccctg
     2641 aggactcact ggaggaatgt gccatcactt gttcaaatag ccacggccct tgtgactcca
     2701 accagcctca caagaacatc aacatcacct ttgaggaaga caaagtcaac tcagctctgg
     2761 ttgtagacag agaatcctct catgatgaat gtcaggatgc tgtaaacatt ctcccagtcc
     2821 ctggccccac ctcttctgcc acaaacgtca gcatggtggt atcagccggc cctttgttca
     2881 gcgagaaggc agagatgaac attctagaaa tcaacgagaa attgcatccc cagctggcag
     2941 agaagaatca gcagttcaga aacctcaaag agaaatgttt tgtaactcaa ctggcctgct
     3001 tcctggccaa ccagcagaac aaatacaaat atgaagagtg caaagacctc ataaaatcta
     3061 tgctgaggaa agagcgacag ttcaaggagg agaagcttgc agagcagctc aagcaagctg
     3121 aggagctcag gcaatataaa gtcctggttc actctcagga acgagagctg acccacttaa
     3181 gggagaagtt acgggaaggg agagatgcct cccgctcatt gaatcagcat ctccaggccc
     3241 tcctcactcc ggataagcca gacaagtccc aggggcagga cctccaagaa cagctggctg
     3301 aggggtgtag actggcacag caacttttcc agaagctcag cccagaaaat gacgaagatg
     3361 aggatgaaga tgttcaagtt gaggaggctg agaaagtact ggaatcatct gcccccaggg
     3421 aggtgcagaa ggctgaagaa agcaaagtcc ctgaggactc actggaggaa tgtgccatca
     3481 cttgttcaaa tagccacagc ccttgtgact ccaaccagcc tcacaagaac atcaacatca
     3541 cctttgagga agacaaagtc aactcaactc tggttgtaga cagagaatcc tctcatgatg
     3601 aatgtcagga tgctgtaaac attctcccag tccctggccc cacctcttct gccacaaacg
     3661 tcagcatggt ggtatcagcc ggccccttgt ccagcgagaa ggcagagatg aacattctag
     3721 aaatcaacga gaaattgcat ccccagctgg cagagaagaa acagcagttc agaaacctca
     3781 aagagaaatg ttttgtaact caactggcct gcttcctggc caaccagcag aacaaataca
     3841 aatatgaaga gtgcaaagac ctcataaaat ctatgctgag gaatgagcga cagttcaagg
     3901 aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat aaagtcctgg
     3961 ttcactctca ggaacgggag ctgacccagt taagggagaa gttacgggaa gggagagatg
     4021 cctcccgctc attgaatcag catctccagg ccctcctcac tccggatgag ccagacaagt
     4081 cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactggca cagcaccttg
     4141 tccaaaagct cagcccagaa aatgacaatg atgacgatga agatgttcaa gttgaggtgg
     4201 ctgagaaagt gcaggaatcg tctgccccca gggagatgcc gaaggctgaa gaaaaggaag
     4261 tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccat ggcccttatg
     4321 actccaacca gccacatagg aaaaccaaaa tcacatttga ggaagacaaa gtcgactcaa
     4381 ctctcattgg ctcatcctct catgttgaat gggaggatgc tgtacacatt atcccagaaa
     4441 atgaaagtga tgatgaggaa gaggaagaaa aagggccagt gtctcccagg aatctgcagg
     4501 agtctgaaga ggaggaagtc ccccaggagt cctgggatga aggttattcg actctctcaa
     4561 ttcctcctga aatgttggcc tcgtaccagt cttacagcgg cacatttcac tcattagagg
     4621 aacagcaagt ctgcatggct gttgacatag gcggacatcg gtgggatcaa gtgaaaaagg
     4681 aggaccaaga ggcaacaggt cccaggctca gcagggagct gctggatgag aaagggcctg
     4741 aagtcttgca ggactcactg gatagatgtt attcaactcc ttcaggttat cttgaactga
     4801 ctgactcatg ccagccctac agaagtgcct tttacatatt ggagcaacag cgtgttggct
     4861 gggctcttga catggatgaa attgaaaagt accaagaagt ggaagaagac caagacccat
     4921 catgccccag gctcagcagg gagctgctgg atgagaaaga gcctgaagtc ttgcaggact
     4981 cactggatag atgttattcg actccttcag gttatcttga actgcctgac ttaggccagc
     5041 cctacagaag tgctgtttac tcattggagg aacagtacct tggcttggct cttgacgtgg
     5101 acagaattaa aaaggaccag gaagaggaag aagaccaagg cccaccatgc cccaggctca
     5161 gcagggagct gctggaggca gtagagcctg aagtcttgca ggactcactg gatagatgtt
     5221 attcaactcc ttccagttgt cttgaacagc ctgactcctg cctgccctat ggaagttcct
     5281 tttatgcatt ggaggaaaaa catgttggct tttctcttga cgtgggagaa attgaaaaga
     5341 aggggaaggg gaagaaaaga aggggaagaa gatcaacgaa gaaaagaagg agaaggggaa
     5401 gaaaagaagg ggaagaagat caaaacccac catgccccag gctcagcagg gagctgctgg
     5461 atgagaaagg gcctgaagtc ttgcaggact cactggatag atgttattca actccttcag
     5521 gttatcttga actgactgac tcatgccagc cctacagaag tgccttttac atattggagc
     5581 aacagcgtgt tggctgggct cttgacatgg atgaaattga aaagtaccaa gaagtggaag
     5641 aagaccaaga cccatcatgc cccaggctca gcagggagct gctggatgag aaagagcctg
     5701 aagtcttgca ggactcactg gatagatgtt attcgactcc ttcaggttat cttgaactgc
     5761 ctgacttagg ccagccctac agaagtgctg tttactcatt ggaggaacag taccttggct
     5821 tggctcttga cgtggacaga attaaaaagg accaggaaga ggaagaagac caaggcccac
     5881 catgccccag gctcagcagg gagctgctgg aggcagtaga gcctgaagtc ttgcaggact
     5941 cactggatag atgttattca actccttcca gttgtcttga acagcctgac tcctgcctgc
     6001 cctatggaag ttccttttat gcattggagg aaaaacatgt tggcttttct cttgacgtgg
     6061 gagaaattga aaagaagggg aaggggaaga aaagaagggg aagaagatca acgaagaaaa
     6121 gaaggagaag gggaagaaaa gaaggggaag aagatcaaaa cccaccatgc cccaggctca
     6181 gcggtgtgct gatggaagtg gaagagcctg aagtcttaca ggactcactg gatagatgtt
     6241 attcgactcc gtcaatgttc tttgaactac ctgactcatt ccagcactac agaagtgtgt
     6301 tttactcatt tgaggaacag cacatcagct tcgcccttga cgtggacaat aggtttctta
     6361 ctttgatggg aacaagtctc cacctggtct tccagatggg agtcatattc ccacagtaag
     6421 cagcccttac taagccgaga gatgtcattc ctgcaggcag gacctatagg cacgtgaaga
     6481 tttgaatgaa actatagttc catttggaag cccagacata ggatgggtca gtgggcatgg
     6541 ctctattcct attctcagac catgccagtg gcaacctgtg ctcagtctga agacaatgga
     6601 cccaagttag gtgtgacacg ttcacataac tgtgcagcac atgccgggag tgatcagtca
     6661 gacattttaa tttgaaccac gtatctctgg gtagctacaa agttcctcag ggatttcatt
     6721 ttgcaggcat gtctctgagc ttctatacct gctcaaggtc agtgtcatct ttgtgtttag
     6781 ctcatccaaa ggtgttaccc tggtttcaat gaacctaacc tcattctttg tatcttcagt
     6841 gttgaattgt tttagctgat ccatctttaa cgcaggaggg atccttggct gaggattgta
     6901 tttcagaacc accaactgct cttgacaatt gttaacccgc taggctcctt tggttagaga
     6961 agccacagtc cttcagcctc caattggtgt cagtacttag gaagaccaca gctagatgga
     7021 caaacagcat tgggaggcct tagccctgct cctctcgatt ccatcctgta gagaacagga
     7081 gtcaggagcc gctggcagga gacagcatgt cacccaggac tctgccggtg cagaatatga
     7141 acaacgccat gttcttgcag aaaacgctta gcctgagttt cataggaggt aatcaccaga
     7201 caactgcaga atgtagaaca ctgagcagga caactgacct gtctccttca catagtccat
     7261 atcaccacaa atcacacaac aaaaaggaga agagatattt tgggttcaaa aaaagtaaaa
     7321 agataatata gctgcatttc tttagttatt ttgaacccca aatatttcct catctttttg
     7381 ttgttgtcat tgatggtggt gacatggact tgtttataga ggacaggtca gctgtctggc
     7441 tcaatgatct acattctgaa gttgtctgaa aatgtcttca tgattaaatt cagcctaaac
     7501 gttttgccgg gaacactgca gagacaatgc tgtgagtttc caaccttagc ccatctgcgg
     7561 gcagagaagg tctagtttgt ccatcagcat tatcatgata tcaggactgg ttacttggtt
     7621 aaggaggggt ctaggagatc tgtccctttt agagacacct tacttataat gaagtatttg
     7681 ggagggtggt tttcaaaagt agaaatgtcc tgtattccga tgatcatcct gtaaacattt
     7741 tatcatttat taatcatccc tgcctgtgtc tattattata ttcatatctc tacgctggaa
     7801 actttctgcc tcaatattta ctgtgccttt gtttttgcta gtttgtgttg ttgaaaaaaa
     7861 aaacattctc tgcctgagtt ttaatttttg tccaaagtta ttttaatcta tacaattaaa
     7921 agcttttgcc tatcactct
//
