LOCUS       NM_001405670            6895 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens NBPF member 1 (NBPF1), transcript variant 5, mRNA.
ACCESSION   NM_001405670
VERSION     NM_001405670.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Li,L., Chen,S., Tang,Y., Wu,J., He,Y. and Qiu,L.
  TITLE     Oncogene or tumor suppressor gene: An integrated pan-cancer
            analysis of NBPF1
  JOURNAL   Front Endocrinol (Lausanne) 13, 950326 (2022)
   PUBMED   36060966
  REMARK    GeneRIF: Oncogene or tumor suppressor gene: An integrated
            pan-cancer analysis of NBPF1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Zhang,J., Zhao,Y., Wang,J., Sneh,T., Yu,Q., Zhou,X. and Gong,C.
  TITLE     NBPF1 independently determine the risk stratification and prognosis
            of patients with neuroblastoma
  JOURNAL   Genomics 112 (6), 3951-3957 (2020)
   PUBMED   32619576
  REMARK    GeneRIF: NBPF1 independently determine the risk stratification and
            prognosis of patients with neuroblastoma.
REFERENCE   3  (bases 1 to 6895)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6895)
  AUTHORS   Gao,Y., Zhu,H. and Mao,Q.
  TITLE     Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on
            growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma
            cells
  JOURNAL   Neoplasma 66 (4), 584-592 (2019)
   PUBMED   31058534
  REMARK    GeneRIF: Upregulation of NBPF1 might promote apoptosis of A431
            squamous carcinoma cells and block cell cycle via inhibiting the
            activation of Akt-p53-Cyclin signaling pathway
REFERENCE   5  (bases 1 to 6895)
  AUTHORS   Qin,Y., Tang,X. and Liu,M.
  TITLE     Tumor-Suppressor Gene NBPF1 Inhibits Invasion and PI3K/mTOR
            Signaling in Cervical Cancer Cells
  JOURNAL   Oncol Res 23 (1-2), 13-20 (2016)
   PUBMED   26802646
  REMARK    GeneRIF: NBPF1 overexpression may be a suppressor for cervical
            cancer via affecting cell invasion and apoptosis through regulating
            PI3K/mTOR signaling pathway.
REFERENCE   6  (bases 1 to 6895)
  AUTHORS   Andries,V., Vandepoele,K., Staes,K., Berx,G., Bogaert,P., Van
            Isterdael,G., Ginneberge,D., Parthoens,E., Vandenbussche,J.,
            Gevaert,K. and van Roy,F.
  TITLE     NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth
            inhibitory effects by inducing a G1 cell cycle arrest
  JOURNAL   BMC Cancer 15, 391 (2015)
   PUBMED   25958384
  REMARK    GeneRIF: We demonstrated that NBPF1 exerts different tumor
            suppressive effects, depending on the cell line analyzed, and
            provide new clues into the molecular mechanism of the enigmatic
            NBPF proteins.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6895)
  AUTHORS   O'Bleness,M.S., Dickens,C.M., Dumas,L.J., Kehrer-Sawatzki,H.,
            Wyckoff,G.J. and Sikela,J.M.
  TITLE     Evolutionary history and genome organization of DUF1220 protein
            domains
  JOURNAL   G3 (Bethesda) 2 (9), 977-986 (2012)
   PUBMED   22973535
REFERENCE   8  (bases 1 to 6895)
  AUTHORS   Vandepoele,K., Staes,K., Andries,V. and van Roy,F.
  TITLE     Chibby interacts with NBPF1 and clusterin, two candidate tumor
            suppressors linked to neuroblastoma
  JOURNAL   Exp Cell Res 316 (7), 1225-1233 (2010)
   PUBMED   20096688
  REMARK    GeneRIF: Chibby and clusterin were co-immunoprecipitated with
            NBPF1.
REFERENCE   9  (bases 1 to 6895)
  AUTHORS   Vandepoele,K., Van Roy,N., Staes,K., Speleman,F. and van Roy,F.
  TITLE     A novel gene family NBPF: intricate structure generated by gene
            duplications during primate evolution
  JOURNAL   Mol Biol Evol 22 (11), 2265-2274 (2005)
   PUBMED   16079250
REFERENCE   10 (bases 1 to 6895)
  AUTHORS   Laureys,G., Speleman,F., Versteeg,R., van der Drift,P., Chan,A.,
            Leroy,J., Francke,U., Opdenakker,G. and Van Roy,N.
  TITLE     Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in
            a neuroblastoma patient. Establishment of somatic cell hybrids and
            identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on
            chromosome 17 as breakpoint flanking single copy markers
  JOURNAL   Oncogene 10 (6), 1087-1093 (1995)
   PUBMED   7700633
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC277909.1 and AC254635.1.
            
            On May 3, 2022 this sequence version replaced NM_001405670.1.
            
            Summary: This gene is a member of the neuroblastoma breakpoint
            family (NBPF) which consists of dozens of recently duplicated genes
            primarily located in segmental duplications on human chromosome 1.
            This gene family has experienced its greatest expansion within the
            human lineage and has expanded, to a lesser extent, among primates
            in general. Members of this gene family are characterized by
            tandemly repeated copies of DUF1220 protein domains. Gene copy
            number variations in the human chromosomal region 1q21.1, where
            most DUF1220 domains are located, have been implicated in a number
            of developmental and neurogenetic diseases such as microcephaly,
            macrocephaly, autism, schizophrenia, cognitive disability,
            congenital heart disease, neuroblastoma, and congenital kidney and
            urinary tract anomalies. Altered expression of some gene family
            members is associated with several types of cancer. This gene
            family contains numerous pseudogenes. [provided by RefSeq, Apr
            2013].
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC277909.1         185116-185342
            228-499             AC277909.1         189897-190168
            500-578             AC277909.1         203688-203766
            579-648             AC277909.1         204036-204105
            649-678             AC277909.1         204942-204971
            679-805             AC277909.1         205128-205254
            806-960             AC277909.1         206382-206536
            961-1170            AC277909.1         206639-206848
            1171-1273           AC277909.1         208694-208796
            1274-1488           AC277909.1         209632-209846
            1489-1561           AC277909.1         210898-210970
            1562-1773           AC277909.1         211434-211645
            1774-1983           AC277909.1         212992-213201
            1984-2086           AC254635.1         129707-129809
            2087-2301           AC254635.1         130645-130859
            2302-2374           AC254635.1         131910-131982
            2375-2586           AC254635.1         132446-132657
            2587-2796           AC254635.1         134007-134216
            2797-2899           AC254635.1         135990-136092
            2900-3114           AC254635.1         136928-137142
            3115-3187           AC254635.1         138180-138252
            3188-3393           AC254635.1         138717-138922
            3394-3445           AC254635.1         140214-140265
            3446-3609           AC254635.1         144172-144335
            3610-3661           AC254635.1         145378-145429
            3662-3834           AC254635.1         146069-146241
            3835-3886           AC254635.1         146961-147012
            3887-4059           AC254635.1         147615-147787
            4060-4111           AC254635.1         148497-148548
            4112-4284           AC254635.1         149171-149343
            4285-4396           AC254635.1         150058-150169
            4397-4569           AC254635.1         150776-150948
            4570-4621           AC254635.1         151668-151719
            4622-4794           AC254635.1         152322-152494
            4795-4846           AC254635.1         153204-153255
            4847-5019           AC254635.1         153872-154044
            5020-5131           AC254635.1         154759-154870
            5132-6895           AC254635.1         160198-161961
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.13"
     gene            1..6895
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="NBPF member 1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
     exon            1..227
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            228..499
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            500..578
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            579..648
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            649..678
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            679..805
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            806..960
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1170
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             996..5375
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="isoform 3 is encoded by transcript variant 5;
                     neuroblastoma breakpoint family member 1; NBPF family
                     member NBPF1"
                     /codon_start=1
                     /product="NBPF family member NBPF1 isoform 3"
                     /protein_id="NP_001392599.1"
                     /db_xref="GeneID:55672"
                     /db_xref="HGNC:HGNC:26088"
                     /db_xref="MIM:610501"
                     /translation="MVVSAGPWSSEKAETNILEINEKLRPQLAEKKQQFRNLKEKCFV
                     TQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQ
                     ERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQ
                     KLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPC
                     DSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSA
                     GPLFSEKAEMNILEINEKLHPQLAEKNQQFRNLKEKCFVTQLACFLANQQNKYKYEEC
                     KDLIKSMLRKERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTHLREKLREGRDASR
                     SLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEA
                     EKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVN
                     STLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKL
                     HPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKL
                     AEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDEPDKSQ
                     GQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQESSAPREMPKAEEKE
                     VPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHII
                     PENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYQSYSGTF
                     HSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTP
                     SGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDE
                     KEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEE
                     EDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKH
                     VGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSRELLDEKGPE
                     VLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQD
                     PSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLA
                     LDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCL
                     PYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCP
                     RLSGVLMEVEEPEVLQDSLDRCYSTPSMFFELPDSFQHYRSVFYSFEEQHISFALDVD
                     NRFLTLMGTSLHLVFQMGVIFPQ"
     exon            1171..1273
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1488
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1561
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1773
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1983
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2086
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2301
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2374
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2586
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2796
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2899
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            2900..3114
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3115..3187
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3188..3393
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3394..3445
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3446..3609
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3610..3661
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3662..3834
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3835..3886
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            3887..4059
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4060..4111
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4112..4284
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4396
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4397..4569
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4570..4621
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4622..4794
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4795..4846
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            4847..5019
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5020..5131
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     exon            5132..6895
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6871..6876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="hexamer: ATTAAA"
     polyA_site      6891
                     /gene="NBPF1"
                     /gene_synonym="AB13; AB14; AB23; AD2; NBG; NBPF"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcag gcgcaggcga ggggctgggt ggcggttgag acagcggcgg tactgggagg
       61 cgtaggtgag ggtcgcgagg ctgcccgagc ttctgagcga gcgcggtgct tttgggaacg
      121 cgggacgggc gatctgcggc gccaggagct gggccgaggc gcggcggcgc ggctgccggc
      181 tgccctgtga atgggaagtt acgcgaagtc cacccagcgt ttctgaggca atctgaaggc
      241 aaatcctgtt tagacccagg cgaaggttcc cggtgacccg ggctctcacc agccaattgt
      301 cccttgccgt cctcctgagg gtgcctggag cttaagcact gtgtgctctt ggcctccaca
      361 ctggggatgc cgctgactcc cactgtccag ggcttccagt ggattctccg aggccctgat
      421 gtagaaactt ccccattggg tgcaccaaga gcagcctcac atggtgtggg ctgacatcaa
      481 gagctgccag atccaacaga tggccaatct ttcctaagct gctcacctta caagaaaacg
      541 aatcgtactg ctaagaattc aaacttcagc agtcatgggg agccttggaa ggagcccgaa
      601 tcactgatgg aattggacag tgcatggaga tggttcagca ggacaaggtg ctgaaaactc
      661 atccccgtgt gacctcaatg tcattggagg acagtgtgga gtgcagagag acatgctttg
      721 aaatgggatt gatccagtcc tccttccttc actaccacat gaatgctggg cagcccaggg
      781 tcaacccacc gcaccctcaa ctcagacggt tacctggcac gctggccaca gtctacctca
      841 ctcttatcag agtctgagct actggcagtg ctttcagctc tgagttcagg cacctcgaac
      901 cttgtttttg tggtgaagga tcctaaagtg ctgtggggag tgatcacatt tttcacaaca
      961 tccctggctc cacctcttct gccacaaacg tcagcatggt ggtatcagct ggcccttggt
     1021 ccagcgagaa ggcagagacg aacattttag aaatcaacga gaaattgcgc ccccagctgg
     1081 cagagaagaa acagcagttc agaaacctca aagagaaatg ttttgtaact caactggccg
     1141 gcttcctggc caaccgacag aagaaataca agtatgaaga gtgtaaagac ctcataaaat
     1201 ttatgctgag gaatgagcga cagttcaagg aggagaagct tgcagagcag ctcaagcaag
     1261 ctgaggagct caggcaatat aaagtcctgg ttcactctca ggaacgggag ctgacccagt
     1321 taagggagaa gttacgggaa gggagagatg cctcccgctc attgaatcag catctccagg
     1381 ccctcctcac tccggataag ccagacaagt cccaggggca ggacctccaa gaacagctgg
     1441 ctgaggggtg tagactggca cagcaacttt tccaaaagct cagcccagaa aatgacgaag
     1501 atgaggatga agatgttcaa gttgaggagg ctgagaaagt actggaatca tctgccccca
     1561 gggaggtgca gaaggctgaa gaaagcaaag tccctgagga ctcactggag gaatgtgcca
     1621 tcacttgttc aaatagccac ggcccttgtg actccaacca gcctcacaag aacatcaaca
     1681 tcacctttga ggaagacaaa gtcaactcag ctctggttgt agacagagaa tcctctcatg
     1741 atgaatgtca ggatgctgta aacattctcc cagtccctgg ccccacctct tctgccacaa
     1801 acgtcagcat ggtggtatca gccggccctt tgttcagcga gaaggcagag atgaacattc
     1861 tagaaatcaa cgagaaattg catccccagc tggcagagaa gaatcagcag ttcagaaacc
     1921 tcaaagagaa atgttttgta actcaactgg cctgcttcct ggccaaccag cagaacaaat
     1981 acaaatatga agagtgcaaa gacctcataa aatctatgct gaggaaagag cgacagttca
     2041 aggaggagaa gcttgcagag cagctcaagc aagctgagga gctcaggcaa tataaagtcc
     2101 tggttcactc tcaggaacga gagctgaccc acttaaggga gaagttacgg gaagggagag
     2161 atgcctcccg ctcattgaat cagcatctcc aggccctcct cactccggat aagccagaca
     2221 agtcccaggg gcaggacctc caagaacagc tggctgaggg gtgtagactg gcacagcaac
     2281 ttttccagaa gctcagccca gaaaatgacg aagatgagga tgaagatgtt caagttgagg
     2341 aggctgagaa agtactggaa tcatctgccc ccagggaggt gcagaaggct gaagaaagca
     2401 aagtccctga ggactcactg gaggaatgtg ccatcacttg ttcaaatagc cacagccctt
     2461 gtgactccaa ccagcctcac aagaacatca acatcacctt tgaggaagac aaagtcaact
     2521 caactctggt tgtagacaga gaatcctctc atgatgaatg tcaggatgct gtaaacattc
     2581 tcccagtccc tggccccacc tcttctgcca caaacgtcag catggtggta tcagccggcc
     2641 ccttgtccag cgagaaggca gagatgaaca ttctagaaat caacgagaaa ttgcatcccc
     2701 agctggcaga gaagaaacag cagttcagaa acctcaaaga gaaatgtttt gtaactcaac
     2761 tggcctgctt cctggccaac cagcagaaca aatacaaata tgaagagtgc aaagacctca
     2821 taaaatctat gctgaggaat gagcgacagt tcaaggagga gaagcttgca gagcagctca
     2881 agcaagctga ggagctcagg caatataaag tcctggttca ctctcaggaa cgggagctga
     2941 cccagttaag ggagaagtta cgggaaggga gagatgcctc ccgctcattg aatcagcatc
     3001 tccaggccct cctcactccg gatgagccag acaagtccca ggggcaggac ctccaagaac
     3061 agctggctga ggggtgtaga ctggcacagc accttgtcca aaagctcagc ccagaaaatg
     3121 acaatgatga cgatgaagat gttcaagttg aggtggctga gaaagtgcag gaatcgtctg
     3181 cccccaggga gatgccgaag gctgaagaaa aggaagtccc tgaggactca ctggaggaat
     3241 gtgccatcac ttgttcaaat agccatggcc cttatgactc caaccagcca cataggaaaa
     3301 ccaaaatcac atttgaggaa gacaaagtcg actcaactct cattggctca tcctctcatg
     3361 ttgaatggga ggatgctgta cacattatcc cagaaaatga aagtgatgat gaggaagagg
     3421 aagaaaaagg gccagtgtct cccaggaatc tgcaggagtc tgaagaggag gaagtccccc
     3481 aggagtcctg ggatgaaggt tattcgactc tctcaattcc tcctgaaatg ttggcctcgt
     3541 accagtctta cagcggcaca tttcactcat tagaggaaca gcaagtctgc atggctgttg
     3601 acataggcgg acatcggtgg gatcaagtga aaaaggagga ccaagaggca acaggtccca
     3661 ggctcagcag ggagctgctg gatgagaaag ggcctgaagt cttgcaggac tcactggata
     3721 gatgttattc aactccttca ggttatcttg aactgactga ctcatgccag ccctacagaa
     3781 gtgcctttta catattggag caacagcgtg ttggctgggc tcttgacatg gatgaaattg
     3841 aaaagtacca agaagtggaa gaagaccaag acccatcatg ccccaggctc agcagggagc
     3901 tgctggatga gaaagagcct gaagtcttgc aggactcact ggatagatgt tattcgactc
     3961 cttcaggtta tcttgaactg cctgacttag gccagcccta cagaagtgct gtttactcat
     4021 tggaggaaca gtaccttggc ttggctcttg acgtggacag aattaaaaag gaccaggaag
     4081 aggaagaaga ccaaggccca ccatgcccca ggctcagcag ggagctgctg gaggcagtag
     4141 agcctgaagt cttgcaggac tcactggata gatgttattc aactccttcc agttgtcttg
     4201 aacagcctga ctcctgcctg ccctatggaa gttcctttta tgcattggag gaaaaacatg
     4261 ttggcttttc tcttgacgtg ggagaaattg aaaagaaggg gaaggggaag aaaagaaggg
     4321 gaagaagatc aacgaagaaa agaaggagaa ggggaagaaa agaaggggaa gaagatcaaa
     4381 acccaccatg ccccaggctc agcagggagc tgctggatga gaaagggcct gaagtcttgc
     4441 aggactcact ggatagatgt tattcaactc cttcaggtta tcttgaactg actgactcat
     4501 gccagcccta cagaagtgcc ttttacatat tggagcaaca gcgtgttggc tgggctcttg
     4561 acatggatga aattgaaaag taccaagaag tggaagaaga ccaagaccca tcatgcccca
     4621 ggctcagcag ggagctgctg gatgagaaag agcctgaagt cttgcaggac tcactggata
     4681 gatgttattc gactccttca ggttatcttg aactgcctga cttaggccag ccctacagaa
     4741 gtgctgttta ctcattggag gaacagtacc ttggcttggc tcttgacgtg gacagaatta
     4801 aaaaggacca ggaagaggaa gaagaccaag gcccaccatg ccccaggctc agcagggagc
     4861 tgctggaggc agtagagcct gaagtcttgc aggactcact ggatagatgt tattcaactc
     4921 cttccagttg tcttgaacag cctgactcct gcctgcccta tggaagttcc ttttatgcat
     4981 tggaggaaaa acatgttggc ttttctcttg acgtgggaga aattgaaaag aaggggaagg
     5041 ggaagaaaag aaggggaaga agatcaacga agaaaagaag gagaagggga agaaaagaag
     5101 gggaagaaga tcaaaaccca ccatgcccca ggctcagcgg tgtgctgatg gaagtggaag
     5161 agcctgaagt cttacaggac tcactggata gatgttattc gactccgtca atgttctttg
     5221 aactacctga ctcattccag cactacagaa gtgtgtttta ctcatttgag gaacagcaca
     5281 tcagcttcgc ccttgacgtg gacaataggt ttcttacttt gatgggaaca agtctccacc
     5341 tggtcttcca gatgggagtc atattcccac agtaagcagc ccttactaag ccgagagatg
     5401 tcattcctgc aggcaggacc tataggcacg tgaagatttg aatgaaacta tagttccatt
     5461 tggaagccca gacataggat gggtcagtgg gcatggctct attcctattc tcagaccatg
     5521 ccagtggcaa cctgtgctca gtctgaagac aatggaccca agttaggtgt gacacgttca
     5581 cataactgtg cagcacatgc cgggagtgat cagtcagaca ttttaatttg aaccacgtat
     5641 ctctgggtag ctacaaagtt cctcagggat ttcattttgc aggcatgtct ctgagcttct
     5701 atacctgctc aaggtcagtg tcatctttgt gtttagctca tccaaaggtg ttaccctggt
     5761 ttcaatgaac ctaacctcat tctttgtatc ttcagtgttg aattgtttta gctgatccat
     5821 ctttaacgca ggagggatcc ttggctgagg attgtatttc agaaccacca actgctcttg
     5881 acaattgtta acccgctagg ctcctttggt tagagaagcc acagtccttc agcctccaat
     5941 tggtgtcagt acttaggaag accacagcta gatggacaaa cagcattggg aggccttagc
     6001 cctgctcctc tcgattccat cctgtagaga acaggagtca ggagccgctg gcaggagaca
     6061 gcatgtcacc caggactctg ccggtgcaga atatgaacaa cgccatgttc ttgcagaaaa
     6121 cgcttagcct gagtttcata ggaggtaatc accagacaac tgcagaatgt agaacactga
     6181 gcaggacaac tgacctgtct ccttcacata gtccatatca ccacaaatca cacaacaaaa
     6241 aggagaagag atattttggg ttcaaaaaaa gtaaaaagat aatatagctg catttcttta
     6301 gttattttga accccaaata tttcctcatc tttttgttgt tgtcattgat ggtggtgaca
     6361 tggacttgtt tatagaggac aggtcagctg tctggctcaa tgatctacat tctgaagttg
     6421 tctgaaaatg tcttcatgat taaattcagc ctaaacgttt tgccgggaac actgcagaga
     6481 caatgctgtg agtttccaac cttagcccat ctgcgggcag agaaggtcta gtttgtccat
     6541 cagcattatc atgatatcag gactggttac ttggttaagg aggggtctag gagatctgtc
     6601 ccttttagag acaccttact tataatgaag tatttgggag ggtggttttc aaaagtagaa
     6661 atgtcctgta ttccgatgat catcctgtaa acattttatc atttattaat catccctgcc
     6721 tgtgtctatt attatattca tatctctacg ctggaaactt tctgcctcaa tatttactgt
     6781 gcctttgttt ttgctagttt gtgttgttga aaaaaaaaac attctctgcc tgagttttaa
     6841 tttttgtcca aagttatttt aatctataca attaaaagct tttgcctatc actct
//
