LOCUS       NM_015909               7278 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens NBAS subunit of NRZ tethering complex (NBAS),
            transcript variant 1, mRNA.
ACCESSION   NM_015909
VERSION     NM_015909.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Granata,V., Pagani,I., Morenghi,E., Schiavone,M.L., Lezzi,A.,
            Ghezzi,S., Vicenzi,E., Poli,G. and Sobacchi,C.
  TITLE     Modulation of NBAS-Related Functions in the Early Response to
            SARS-CoV-2 Infection
  JOURNAL   Int J Mol Sci 24 (3), 2634 (2023)
   PUBMED   36768954
  REMARK    GeneRIF: Modulation of NBAS-Related Functions in the Early Response
            to SARS-CoV-2 Infection.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Bi,X., Zhang,Q., Chen,L., Liu,D., Li,Y., Zhao,X., Zhang,Y.,
            Zhang,L., Liu,J., Wu,C., Li,Z., Zhao,Y., Ma,H., Huang,G., Liu,X.,
            Wang,Q.F. and Zhang,R.
  TITLE     NBAS, a gene involved in cytotoxic degranulation, is recurrently
            mutated in pediatric hemophagocytic lymphohistiocytosis
  JOURNAL   J Hematol Oncol 15 (1), 101 (2022)
   PUBMED   35902954
  REMARK    GeneRIF: NBAS, a gene involved in cytotoxic degranulation, is
            recurrently mutated in pediatric hemophagocytic
            lymphohistiocytosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7278)
  AUTHORS   Lenz,D., Pahl,J., Hauck,F., Alameer,S., Balasubramanian,M.,
            Baric,I., Boy,N., Church,J.A., Crushell,E., Dick,A.,
            Distelmaier,F., Gujar,J., Indolfi,G., Lurz,E., Peters,B.,
            Schwerd,T., Serranti,D., Kolker,S., Klein,C., Hoffmann,G.F.,
            Prokisch,H., Greil,J., Cerwenka,A., Giese,T. and Staufner,C.
  TITLE     NBAS Variants Are Associated with Quantitative and Qualitative NK
            and B Cell Deficiency
  JOURNAL   J Clin Immunol 41 (8), 1781-1793 (2021)
   PUBMED   34386911
  REMARK    GeneRIF: NBAS Variants Are Associated with Quantitative and
            Qualitative NK and B Cell Deficiency.
REFERENCE   4  (bases 1 to 7278)
  AUTHORS   Cotrina-Vinagre,F.J., Rodriguez-Garcia,M.E., Martin-Hernandez,E.,
            Duran-Aparicio,C., Merino-Lopez,A., Medina-Benitez,E. and
            Martinez-Azorin,F.
  TITLE     Characterization of a complex phenotype (fever-dependent recurrent
            acute liver failure and osteogenesis imperfecta) due to NBAS and
            P4HB variants
  JOURNAL   Mol Genet Metab 133 (2), 201-210 (2021)
   PUBMED   33707149
  REMARK    GeneRIF: Characterization of a complex phenotype (fever-dependent
            recurrent acute liver failure and osteogenesis imperfecta) due to
            NBAS and P4HB variants.
REFERENCE   5  (bases 1 to 7278)
  AUTHORS   Krishnan,S., Rughani,A., Tsai,A. and Palle,S.
  TITLE     Novel compound heterozygous variants in the NBAS gene in a child
            with osteogenesis imperfecta and recurrent acute liver failure
  JOURNAL   BMJ Case Rep 14 (2), e234993 (2021)
   PUBMED   33542026
  REMARK    GeneRIF: Novel compound heterozygous variants in the NBAS gene in a
            child with osteogenesis imperfecta and recurrent acute liver
            failure.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7278)
  AUTHORS   Hirose,H., Arasaki,K., Dohmae,N., Takio,K., Hatsuzawa,K.,
            Nagahama,M., Tani,K., Yamamoto,A., Tohyama,M. and Tagaya,M.
  TITLE     Implication of ZW10 in membrane trafficking between the endoplasmic
            reticulum and Golgi
  JOURNAL   EMBO J 23 (6), 1267-1278 (2004)
   PUBMED   15029241
REFERENCE   7  (bases 1 to 7278)
  AUTHORS   Scott,D.K., Board,J.R., Lu,X., Pearson,A.D., Kenyon,R.M. and
            Lunec,J.
  TITLE     The neuroblastoma amplified gene, NAG: genomic structure and
            characterisation of the 7.3 kb transcript predominantly expressed
            in neuroblastoma
  JOURNAL   Gene 307, 1-11 (2003)
   PUBMED   12706883
REFERENCE   8  (bases 1 to 7278)
  AUTHORS   Kondoh,S., Sugawara,H., Harada,N., Matsumoto,N., Ohashi,H.,
            Sato,M., Kantaputra,P.N., Ogino,T., Tomita,H., Ohta,T., Kishino,T.,
            Fukushima,Y., Niikawa,N. and Yoshiura,K.
  TITLE     A novel gene is disrupted at a 14q13 breakpoint of t(2;14) in a
            patient with mirror-image polydactyly of hands and feet
  JOURNAL   J Hum Genet 47 (3), 136-139 (2002)
   PUBMED   11954550
REFERENCE   9  (bases 1 to 7278)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
REFERENCE   10 (bases 1 to 7278)
  AUTHORS   Wimmer,K., Zhu,X.X., Lamb,B.J., Kuick,R., Ambros,P.F., Kovar,H.,
            Thoraval,D., Motyka,S., Alberts,J.R. and Hanash,S.M.
  TITLE     Co-amplification of a novel gene, NAG, with the N-myc gene in
            neuroblastoma
  JOURNAL   Oncogene 18 (1), 233-238 (1999)
   PUBMED   9926938
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB621813.1, BC108693.1,
            AL832774.1, BC131735.1 and AF388385.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015909.3.
            
            Summary: This gene encodes a protein with two leucine zipper
            domains, a ribosomal protein S14 signature domain and a Sec39 like
            domain. The protein is thought to be involved in Golgi-to-ER
            transport. Mutations in this gene are associated with short
            stature, optic nerve atrophy, and Pelger-Huet anomaly. [provided by
            RefSeq, Oct 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and is protein-coding.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF388385.1, BC131735.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281513.10/ ENSP00000281513.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-756               AB621813.1         15-770
            757-1271            BC108693.1         746-1260
            1272-2253           AL832774.1         1229-2210
            2254-7212           BC131735.1         2250-7208
            7213-7278           AF388385.1         7209-7274
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p24.3"
     gene            1..7278
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /note="NBAS subunit of NRZ tethering complex"
                     /db_xref="GeneID:51594"
                     /db_xref="HGNC:HGNC:15625"
                     /db_xref="MIM:608025"
     exon            1..147
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..7146
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /note="NAG/BC035112 fusion; NAG/FAM49A fusion;
                     neuroblastoma-amplified gene protein; neuroblastoma
                     amplified sequence"
                     /codon_start=1
                     /product="NBAS subunit of NRZ tethering complex"
                     /protein_id="NP_056993.2"
                     /db_xref="CCDS:CCDS1685.1"
                     /db_xref="GeneID:51594"
                     /db_xref="HGNC:HGNC:15625"
                     /db_xref="MIM:608025"
                     /translation="MAAPESGPALSPGTAEGEEETILYDLLVNTEWPPETEVQPRGNQ
                     KHGASFIITKAIRDRLLFLRQYIWYSPAPFLLPDGLVRLVNKQINWHLVLASNGKLLA
                     AVQDQCVEIRSAKDDFTSIIGKCQVPKDPKPQWRRVAWSYDCTLLAYAESTGTVRVFD
                     LMGSELFVISPASSFIGDLSYAIAGLIFLEYKASAQWSAELLVINYRGELRSYLVSVG
                     TNQSYQESHCFSFSSHYPHGINTAIYHPGHRLLLVGGCETAEVGMSKASSCGLSAWRV
                     LSGSPYYKQVTNGGDGVTAVPKTLGLLRMLSVKFYSRQGQEQDGIFKMSLSPDGMLLA
                     AIHFSGKLSIWAIPSLKQQGEWGQNEQPGYDDLNPDWRLSTEKRKKIKDKESFYPLID
                     VNWWADSAVTLARCSGALTVSSVKTLKNLLGKSCEWFEPSPQVTATHDGGFLSLECEI
                     KLAPKRSRLETRAGEEDEGEEDSDSDYEISAKARYFGYIKQGLYLVTEMERFAPPRKR
                     PRTITKNYRLVSLRSTTPEELYQRKIESEEYEEALSLAHTYGLDTDLVYQRQWRKSAV
                     NVASIQNYLSKIKKRSWVLHECLERVPENVDAAKELLQYGLKGTDLEALLAIGKGADD
                     GRFTLPGEIDIDSISYEELSPPDEEPAKNKKEKELKKRQELLKLVNFSKLTLEQKELC
                     RCRRKLLTYLDRLATYEEILGVPHASEQRYDAEFFKKFRNQNIVLSARTYAQESNVQA
                     LEILFTYHGSDLLPHRLAILSNFPETTSPHEYSVLLPEACFNGDSLMIIPWHEHKHRA
                     KDWCEELACRMVVEPNLQDESEFLYAAQPELLRFRMTQLTVEKVMDWYQTRAEEIEHY
                     ARQVDCALSLIRLGMERNIPGLLVLCDNLVTLETLVYEARCDVTLTLKELQQMKDIEK
                     LRLLMNSCSEDKYVTSAYQWMVPFLHRCEKQSPGVANELLKEYLVTLAKGDLKFPLKI
                     FQHSKPDLQQKIIPDQDQLMAIALECIYTCERNDQLCLCYDLLECLPERGYGDKTEAT
                     TKLHDMVDQLEQILSVSELLEKHGLEKPISFVKNTQSSSEEARKLMVRLTRHTGRKQP
                     PVSESHWRTLLQDMLTMQQNVYTCLDSDACYEIFTESLLCSSRLENIHLAGQMMHCSA
                     CSENPPAGIAHKGKPHYRVSYEKSIDLVLAASREYFNSSTNLTDSCMDLARCCLQLIT
                     DRPPAIQEELDLIQAVGCLEEFGVKILPLQVRLCPDRISLIKECISQSPTCYKQSTKL
                     LGLAELLRVAGENPEERRGQVLILLVEQALRFHDYKAASMHCQELMATGYPKSWDVCS
                     QLGQSEGYQDLATRQELMAFALTHCPPSSIELLLAASSSLQTEILYQRVNFQIHHEGG
                     ENISASPLTSKAVQEDEVGVPGSNSADLLRWTTATTMKVLSNTTTTTKAVLQAVSDGQ
                     WWKKSLTYLRPLQGQKCGGAYQIGTTANEDLEKQGCHPFYESVISNPFVAESEGTYDT
                     YQHVPVESFAEVLLRTGKLAEAKNKGEVFPTTEVLLQLASEALPNDMTLALAYLLALP
                     QVLDANRCFEKQSPSALSLQLAAYYYSLQIYARLAPCFRDKCHPLYRADPKELIKMVT
                     RHVTRHEHEAWPEDLISLTKQLHCYNERLLDFTQAQILQGLRKGVDVQRFTADDQYKR
                     ETILGLAETLEESVYSIAISLAQRYSVSRWEVFMTHLEFLFTDSGLSTLEIENRAQDL
                     HLFETLKTDPEAFHQHMVKYIYPTIGGFDHERLQYYFTLLENCGCADLGNCAIKPETH
                     IRLLKKFKVVASGLNYKKLTDENMSPLEALEPVLSSQNILSISKLVPKIPEKDGQMLS
                     PSSLYTIWLQKLFWTGDPHLIKQVPGSSPEWLHAYDVCMKYFDRLHPGDLITVVDAVT
                     FSPKAVTKLSVEARKEMTRKAIKTVKHFIEKPRKRNSEDEAQEAKDSKVTYADTLNHL
                     EKSLAHLETLSHSFILSLKNSEQETLQKYSHLYDLSRSEKEKLHDEAVAICLDGQPLA
                     MIQQLLEVAVGPLDISPKDIVQSAIMKIISALSGGSADLGGPRDPLKVLEGVVAAVHA
                     SVDKGEELVSPEDLLEWLRPFCADDAWPVRPRIHVLQILGQSFHLTEEDSKLLVFFRT
                     EAILKASWPQRQVDIADIENEENRYCLFMELLESSHHEAEFQHLVLLLQAWPPMKSEY
                     VITNNPWVRLATVMLTRCTMENKEGLGNEVLKMCRSLYNTKQMLPAEGVKELCLLLLN
                     QSLLLPSLKLLLESRDEHLHEMALEQITAVTTVNDSNCDQELLSLLLDAKLLVKCVST
                     PFYPRIVDHLLASLQQGRWDAEELGRHLREAGHEAEAGSLLLAVRGTHQAFRTFSTAL
                     RAAQHWV"
     exon            148..202
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            203..239
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            240..317
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            318..365
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            366..409
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            410..543
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            544..677
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            678..776
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            777..915
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            916..984
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1113
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1177
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1371
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1629
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1755
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1907
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2048
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2127
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2232
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2369
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2453
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2607
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2793
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2967
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            2968..3101
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3102..3164
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3287
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3288..3390
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3391..3620
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3621..3733
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3734..3847
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3848..3961
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            3962..4119
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4120..4209
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4210..4377
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4378..4491
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4492..4612
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4613..4689
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4690..4827
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            4828..5057
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            5058..5168
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            5169..5419
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            5420..5754
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            5755..5973
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            5974..6176
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            6177..6266
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            6267..6462
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            6463..6602
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            6603..6741
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            6742..6870
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     exon            6871..7278
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7258..7263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /note="hexamer: AATAAA"
     polyA_site      7278
                     /gene="NBAS"
                     /gene_synonym="ILFS2; NAG; SOPH"
                     /note="major polyA site"
ORIGIN      
        1 actcctccgc tgcctgagtc ctcggcgaac atggcggccc ccgagtcagg gccggctttg
       61 agtccaggca ctgcagaggg tgaggaggag acgattctct atgacttgtt ggtcaacacc
      121 gagtggccac cggagactga agtacagcct agaggcaacc aaaaacatgg tgcatccttt
      181 atcatcacga aagcaattcg agatcgttta ttatttttac gccaatacat ctggtacagc
      241 ccggcacctt ttttgctccc tgatggactg gttcgcttgg ttaataaaca gataaactgg
      301 catttggtac ttgcaagcaa tggaaagctt ttggctgctg ttcaagatca gtgtgtggaa
      361 atcaggtctg caaaagatga ttttacatcc attattggga aatgtcaagt tccgaaagac
      421 ccgaaacccc agtggagacg ggtagcatgg agttacgatt gtaccctact ggcctatgcc
      481 gaaagcacag gaactgtgag ggtgtttgat ctcatgggaa gtgaactctt tgtcatttcc
      541 ccggcatcta gttttatagg tgacttaagc tatgccattg ctgggttgat atttttagaa
      601 tataaagcaa gtgcacagtg gtctgcagaa ctcctggtca tcaattaccg aggagaactt
      661 agaagttacc ttgtaagtgt tggaacaaat cagagctacc aagaaagtca ctgtttcagc
      721 ttcagtagtc attatcctca tggaatcaac acagctattt accaccctgg tcacagactt
      781 ttacttgttg gtggatgtga aactgctgaa gtaggcatgt caaaagcttc tagctgtggc
      841 ctttctgcct ggagagttct ttcaggatca ccgtattata agcaggttac taatggtgga
      901 gacggggtta ctgcagtacc gaagacactg ggattattaa ggatgttaag tgtcaagttt
      961 tacagtcgcc agggacaaga acaggatgga atttttaaga tgagcctttc tccagatggg
     1021 atgctcctgg cagccattca cttctcaggg aaactgagca tctgggcgat tccatctctg
     1081 aagcaacaag gggaatgggg tcaaaatgag cagccaggct atgatgacct taatcctgat
     1141 tggaggctct ctactgagaa gagaaaaaaa atcaaagata aagagtcctt ttacccactg
     1201 atagatgtca attggtgggc agacagtgca gtgactttag ctcgatgctc tggtgcttta
     1261 actgtttcat ctgtgaaaac tttgaagaat ttactgggaa aatcctgtga atggtttgaa
     1321 ccatcacctc aagtcactgc tacccatgat gggggatttt taagtttgga gtgtgagatt
     1381 aaacttgccc ccaaacgatc tcgtttggag actagagctg gagaagaaga tgaaggagaa
     1441 gaggattctg attctgatta tgaaatatct gccaaggctc gctactttgg ttatataaaa
     1501 cagggccttt acttggtgac tgaaatggag cgatttgcac caccacggaa acgcccacga
     1561 accattacta aaaactaccg ccttgtgagt ttgcgctcca cgacaccaga ggaactttat
     1621 cagaggaaga ttgaaagtga agagtatgag gaagccttgt ccttggctca tacctacggc
     1681 ctggatactg accttgtata tcagaggcag tggaggaagt cagcggtcaa cgttgcttca
     1741 attcagaatt atttgagtaa aataaagaag cgatcctggg ttctccatga gtgtttggaa
     1801 agagttcctg aaaatgtgga tgctgcaaaa gaactgcttc agtatggatt aaaaggcaca
     1861 gacctggagg ctcttttagc aatagggaaa ggagcagatg atggcagatt tacattacct
     1921 ggtgaaatag acattgacag tatctcctat gaagagcttt caccacctga tgaagagcct
     1981 gccaagaata aaaaggaaaa ggagctcaag aagagacaag aactactgaa attagtgaac
     2041 ttttccaagt tgacactgga acaaaaggaa ctttgccgtt gtagacggaa gttattaacc
     2101 tacttagatc gacttgcaac atatgaggaa atcctaggag tgcctcatgc atctgaacag
     2161 agatatgatg ctgaattctt taagaaattc agaaatcaga atattgttct ctcagcaaga
     2221 acttatgctc aggaaagtaa tgtacaagcc ctggaaattc tgtttactta ccatggttcc
     2281 gacctgcttc ctcatcgcct tgcaattctg tccaactttc cagagaccac ttctccacat
     2341 gaatattctg ttttgctgcc cgaagcttgt tttaacggtg actccctgat gatcattcct
     2401 tggcatgaac ataaacaccg agctaaagat tggtgcgagg agttggcttg cagaatggtt
     2461 gttgagccga atctccaaga tgaaagtgaa ttcttgtatg ctgcacagcc tgagttacta
     2521 aggttcagga tgacccagct tacggtggag aaggttatgg actggtatca gaccagagca
     2581 gaggaaatag agcattatgc tcggcaggtg gactgtgcat tgtcacttat tcgacttggg
     2641 atggagcgga atattcctgg tttgctggtt ctctgtgaca atttggttac tctggaaaca
     2701 ttggtttatg aagccaggtg tgatgtaact ctaaccctga aagaactcca gcagatgaaa
     2761 gacattgaaa aactaagatt actgatgaat agttgttctg aggataaata tgtgacaagt
     2821 gcctaccagt ggatggttcc ctttcttcat cgttgtgaga aacagtcgcc tggtgtggct
     2881 aatgagctat taaaagaata tttagtaact ttagctaaag gggacttaaa atttcccctg
     2941 aagatatttc agcattccaa accagatctg cagcaaaaaa ttattcctga tcaggaccaa
     3001 ctgatggcaa tagcactaga gtgcatctat acctgtgaac gaaatgatca actctgtctt
     3061 tgctatgacc tactagaatg tctgccagaa agaggatatg gtgataagac agaggcaacc
     3121 acaaagcttc atgacatggt agaccaactg gaacaaattc tcagtgtgtc agagcttttg
     3181 gaaaaacatg gactcgagaa accaatttca tttgttaaaa acactcaatc tagctcagaa
     3241 gaggcacgca agctgatggt tagattgacg aggcacactg gccggaagca gcctcctgtc
     3301 agtgagtctc attggagaac gttgctgcaa gacatgttaa ctatgcagca gaatgtatac
     3361 acatgtctag attctgatgc ctgctatgag atatttacag aaagccttct gtgctctagt
     3421 cgccttgaaa acatccacct ggctggacag atgatgcact gcagtgcttg ttcagaaaat
     3481 cctccagctg gtatagccca taaagggaaa ccccactaca gggtcagcta cgaaaagagt
     3541 attgacttgg ttttggctgc cagcagagag tacttcaatt cttctaccaa cctcactgat
     3601 agctgcatgg atctagccag gtgctgctta caactgataa cagacagacc ccctgccatt
     3661 caagaggagc tagatcttat ccaagccgtt ggatgtcttg aagaatttgg ggtaaagatc
     3721 ctgcctttgc aagtgcgatt gtgccctgat cggatcagtc tcatcaagga gtgtatttcc
     3781 cagtccccca catgctataa acaatccacc aagcttctgg gccttgctga gctgctgagg
     3841 gttgcaggtg agaacccaga agaaaggcgg ggacaggttc taatcctttt agtggagcag
     3901 gcacttcgct tccatgacta caaagcagcc agtatgcatt gtcaggagct gatggccaca
     3961 ggttatccta aaagttggga tgtttgtagc cagttaggac aatcagaagg ttaccaggac
     4021 ttggccactc gtcaagagct catggctttt gctttgacac attgccctcc tagcagcatt
     4081 gaacttcttt tggcagctag cagctctctg cagacagaaa ttctttatca aagagtgaat
     4141 ttccagatcc atcatgaagg aggggaaaat atcagtgctt caccattaac tagtaaagca
     4201 gtacaagagg atgaagtagg tgttccaggt agcaattcag ctgacctatt gcgctggacc
     4261 actgctacca ccatgaaagt cctttccaac accacaacca ccaccaaagc ggtgctgcag
     4321 gccgtcagtg atgggcagtg gtggaagaag tctttaactt accttcgacc ccttcagggg
     4381 caaaaatgtg gtggtgcata tcaaatcgga actacagcca atgaagatct agagaaacaa
     4441 gggtgtcatc ctttttatga atctgtcatc tcaaatcctt ttgtcgctga gtctgaaggg
     4501 acctatgaca cctatcagca tgttccagtg gaaagctttg cagaagtatt gctgagaact
     4561 ggaaaattgg cagaggctaa aaataaagga gaagtatttc caacaactga agttctcttg
     4621 caactagcaa gtgaagcctt gccaaatgac atgaccttgg ctcttgctta ccttcttgcc
     4681 ttaccacaag tgttagatgc taaccggtgc tttgaaaagc agtccccctc tgcattatct
     4741 ctccagctgg cagcgtatta ctatagcctc cagatctatg cccgattggc cccatgtttc
     4801 agggacaagt gccatcctct ttacagggct gatcccaaag aactaatcaa gatggtcacc
     4861 aggcatgtga ctcgacatga gcacgaagcc tggcctgaag accttatttc actgaccaag
     4921 cagttacact gctacaatga acgtctcctg gatttcactc aggcgcagat ccttcagggc
     4981 cttcggaagg gtgtggacgt gcagcggttt actgcagatg accagtataa aagggaaact
     5041 atccttggtc tggcagaaac tctagaggaa agcgtctaca gcattgctat ttctctggca
     5101 caacgttaca gtgtctcccg ctgggaagtt tttatgaccc atttggagtt cctcttcacg
     5161 gacagtggtt tgtccacact agaaattgaa aatagagccc aagaccttca tctctttgag
     5221 actttgaaga ctgatccaga agcctttcac cagcacatgg tcaagtatat ttaccctact
     5281 attggtggct ttgatcacga aaggctgcag tattatttca ctcttctgga aaactgtggc
     5341 tgtgcagatt tggggaactg tgccattaaa ccagaaaccc acattcgact gctgaagaag
     5401 tttaaggttg ttgcatcagg tcttaattac aaaaagctga cagatgaaaa catgagtcct
     5461 cttgaagcat tggagccagt tctttcaagt caaaatatct tgtctatttc caaacttgtt
     5521 cccaaaatcc ctgaaaagga tggacagatg ctttccccaa gctctctgta caccatctgg
     5581 ttacagaagt tgttctggac tggagaccct catctcatta aacaagtccc aggctcttca
     5641 ccggagtggc ttcatgccta tgatgtctgc atgaagtact ttgatcgtct ccacccaggt
     5701 gacctcatca ctgtggtaga tgcagttaca ttttctccaa aagctgtgac caagctgtct
     5761 gtggaagccc gtaaagagat gactagaaag gctattaaga cagtcaaaca ttttattgag
     5821 aagccaagga aaagaaactc agaagacgaa gctcaagaag ctaaggattc taaagttacc
     5881 tatgcagata ctttgaatca tctggagaaa tcacttgccc acctggaaac cctgagccac
     5941 agcttcatcc tttctctgaa gaatagtgag caggaaacac tgcaaaaata cagtcacctc
     6001 tatgatctgt cccgatcaga aaaagagaaa cttcatgatg aagctgtggc tatttgttta
     6061 gatggtcagc ctctagcaat gattcagcag ctgctagagg tggcagttgg ccctcttgac
     6121 atctcaccca aggatatagt gcagagtgca atcatgaaaa taatttctgc attgagtggt
     6181 ggcagtgctg accttggtgg gccaagggac ccactgaagg tcctggaagg tgttgttgca
     6241 gcagtccacg ccagtgtgga caagggtgag gagctggttt cacctgagga cctgctggag
     6301 tggctgcggc ctttctgtgc tgatgacgcc tggccggtgc ggccccgcat tcacgtgctg
     6361 cagattttgg ggcaatcatt tcacctgact gaggaggaca gcaagctcct cgtgttcttt
     6421 agaactgaag ccattctcaa agcctcctgg ccccagagac aggtagacat agctgacatt
     6481 gagaatgaag agaaccgcta ctgtctattc atggaactcc tggaatctag tcaccacgag
     6541 gctgaatttc agcacttggt tttacttttg caagcttggc cacctatgaa aagtgaatat
     6601 gtcataacca ataatccatg ggtgagacta gctacagtga tgctaaccag atgtacgatg
     6661 gagaacaagg aaggattggg gaatgaagtt ttgaaaatgt gtcgctcttt gtataacacc
     6721 aagcagatgc tgcctgcaga gggtgtgaag gagctgtgtc tgctgctgct taaccagtcc
     6781 ctcctgcttc catctctgaa acttctcctc gagagccgag atgagcatct gcacgagatg
     6841 gcactggagc aaatcacggc agtcactacg gtgaatgatt ccaattgtga ccaagaactt
     6901 ctttccctgc tcctggatgc caagctgctg gtgaagtgtg tctccactcc cttctatcca
     6961 cgtattgttg accacctctt ggctagcctc cagcaagggc gctgggatgc agaggagctg
     7021 ggcagacacc tgcgggaggc cggccatgaa gccgaagccg ggtctctcct tctggccgtg
     7081 agggggactc accaggcctt cagaaccttc agtacagccc tccgcgcagc acagcactgg
     7141 gtgtgagggc cacctgtggc cctgctcctt agcagaaaaa gcatctggag ttgaatgctg
     7201 ttcccagaag caacatgtgt atctgccgat tgttctccat ggttccaaca aattgcaaat
     7261 aaaactgtat ggaaacga
//
